Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model.
Abstract: Several studies report beneficial effects of autologous and allogeneic stem cells on wound healing. However, no comparison between autologous versus allogeneic epithelial-like stem cells (EpSCs) has been made so far. For this reason, we first hypothesize that both EpSC types enhance wound healing in comparison to vehicle treatment and untreated controls. Second, on the basis of other studies, we hypothesized that there would be no difference between autologous and allogeneic EpSCs. Methods: Twelve full-thickness skin wounds were created in six horses. Each horse was subjected to (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Wound evaluation was performed at day 3, 7 and 14 through wound exudates and at week 1, 2 and 5 through biopsies. Results: Wound circumference and surface were significantly smaller in autologous EpSC-treated wounds. A significantly lower amount of total granulation tissue (overall) and higher vascularization (week 1) was observed after both EpSC treatments. Significantly more major histocompatibility complex II-positive and CD20-positive cells were noticed in EpSC-treated wounds at week 2. In autologous and allogeneic groups, the number of EpSCs in center biopsies was low after 1 week (11.7% and 6.1%), decreased to 7.6% and 1.7%, respectively (week 2), and became undetectable at week 5. Conclusions: These results confirm the first hypothesis and partially support the second hypothesis. Besides macroscopic improvements, both autologous and allogeneic EpSCs had similar effects on granulation tissue formation, vascularization and early cellular immune response.
Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
Publication Date: 2015-07-23 PubMed ID: 26212608DOI: 10.1016/j.jcyt.2015.06.004Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study focuses on comparing the effects of using personal (autologous) versus donated (allogeneic) epithelial-like stem cells (EpSCs) in healing skin wounds on horses. The study found that while both types of EpSCs improved wound healing, wounds treated with autologous EpSCs had a significantly smaller circumference and surface.
Study Design and Methodology
- The study involved the creation of twelve full-thickness skin wounds on six horses. Various treatments were then applied: Autologous EpSCs, Allogeneic EpSCs, vehicle treatment (presumably a placebo), and an untreated control group.
- The evaluation of wound healing occurred at multiple time points. Day 3, 7, and 14 assessments analyzed wound exudates while week 1, 2, and 5 evaluations used biopsies.
Results of the Study
- The wounds treated with autologous EpSCs had significantly smaller circumference and surface, indicating superior healing than both allogeneic treatment and controls.
- Both autologous and allogeneic EpSC treatments resulted in a significantly lower amount of total granulation tissue (important in wound healing) overall and higher blood vessel formation (vascularization) in week 1.
- There were significantly more immune cells, specifically major histocompatibility complex II-positive and CD20-positive cells, in wounds that received EpSC treatments at week 2.
- This study found diminishing presence of autologous and allogeneic EpSCs in the central biopsy over time. Initial values dropped from 11.7% and 6.1% respectively after week 1 to 7.6% and 1.7% respectively at week 2, becoming undetectable by week 5.
Conclusions of the Study
- Results of the study confirmed the first hypothesis: both types of EpSCs improve wound healing relative to vehicle treatment and untreated controls.
- The outcomes partially support the second hypothesis: while there is prevalent similarity between autologous and allogeneic EpSCs in affecting tissue formation, vascularization, and early cellular immune response, autologous EpSCs appear superior in reducing wound size.
Cite This Article
APA
Broeckx SY, Borena BM, Van Hecke L, Chiers K, Maes S, Guest DJ, Meyer E, Duchateau L, Martens A, Spaas JH.
(2015).
Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model.
Cytotherapy, 17(10), 1434-1446.
https://doi.org/10.1016/j.jcyt.2015.06.004 Publication
Researcher Affiliations
- Global Stem Cell Technology, ANACURA Group, Evergem, Belgium; Pell Cell Medicals, ANACURA Group, Evergem, Belgium.
- Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Department of Veterinary Laboratory, College of Agriculture and Veterinary Science, Ambo University, Ethiopia.
- Department of Surgery and Anesthesia of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
- Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
- Vet Med Lab, IDEXX Laboratories, Brussels, Belgium.
- Centre for Preventive Medicine, Animal Health Trust, Newmarket, Suffolk, United Kingdom.
- Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
- Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
- Department of Surgery and Anesthesia of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
- Global Stem Cell Technology, ANACURA Group, Evergem, Belgium; Pell Cell Medicals, ANACURA Group, Evergem, Belgium. Electronic address: jan.spaas@anacura.com.
MeSH Terms
- Animals
- Antigens, CD20 / metabolism
- Epithelial Cells / cytology
- Female
- Histocompatibility Antigens Class II / immunology
- Horses
- Humans
- Neovascularization, Physiologic / physiology
- Skin / blood supply
- Skin / injuries
- Stem Cell Transplantation / methods
- Stem Cells / cytology
- Transplantation, Autologous
- Transplantation, Homologous
- Wound Healing / physiology
Citations
This article has been cited 10 times.- Ribeiro G, Carvalho L, Borges J, Prazeres J. The Best Protocol to Treat Equine Skin Wounds by Second Intention Healing: A Scoping Review of the Literature. Animals (Basel) 2024 May 18;14(10).
- Iacopetti I, Perazzi A, Patruno M, Contiero B, Carolo A, Martinello T, Melotti L. Assessment of the quality of the healing process in experimentally induced skin lesions treated with autologous platelet concentrate associated or unassociated with allogeneic mesenchymal stem cells: preliminary results in a large animal model. Front Vet Sci 2023;10:1219833.
- Di Francesco P, Cajon P, Desterke C, Perron Lepage MF, Lataillade JJ, Kadri T, Lepage OM. Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair. Vet Med Int 2021;2021:5024905.
- Vera L, Muylle S, Van Steenkiste G, Segers P, Decloedt A, Chiers K, van Loon G. Histological and biomechanical properties of systemic arteries in young and old Warmblood horses. PLoS One 2021;16(7):e0253730.
- Enciso N, Avedillo L, Fermín ML, Fragío C, Tejero C. Cutaneous wound healing: canine allogeneic ASC therapy. Stem Cell Res Ther 2020 Jun 29;11(1):261.
- Mund SJK, Kawamura E, Awang-Junaidi AH, Campbell J, Wobeser B, MacPhee DJ, Honaramooz A, Barber S. Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project. Cells 2020 May 8;9(5).
- He B, Chen J, Liu L, Wang H, Wang S, Li P, Zhou J. Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats. Biosci Rep 2019 Aug 30;39(8).
- Broeckx SY, Martens AM, Bertone AL, Van Brantegem L, Duchateau L, Van Hecke L, Dumoulin M, Oosterlinck M, Chiers K, Hussein H, Pille F, Spaas JH. The use of equine chondrogenic-induced mesenchymal stem cells as a treatment for osteoarthritis: A randomised, double-blinded, placebo-controlled proof-of-concept study. Equine Vet J 2019 Nov;51(6):787-794.
- Martinello T, Gomiero C, Perazzi A, Iacopetti I, Gemignani F, DeBenedictis GM, Ferro S, Zuin M, Martines E, Brun P, Maccatrozzo L, Chiers K, Spaas JH, Patruno M. Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin. BMC Vet Res 2018 Jun 25;14(1):202.
- Textor JA, Clark KC, Walker NJ, Aristizobal FA, Kol A, LeJeune SS, Bledsoe A, Davidyan A, Gray SN, Bohannon-Worsley LK, Woolard KD, Borjesson DL. Allogeneic Stem Cells Alter Gene Expression and Improve Healing of Distal Limb Wounds in Horses. Stem Cells Transl Med 2018 Jan;7(1):98-108.
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