Comparison of diagnostic tests for the detection of equine infectious anemia antibody.
Abstract: Two diagnostic tests are approved for detecting antibody to equine infectious anemia virus: the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA). A total of 420 sera from National Veterinary Services Laboratories check sets were tested with the AGID and competitive ELISA. A 100% correlation was obtained. The AGID and competitive ELISA were further used to test difficult samples with low levels of equine infectious anemia antibody (weak positives). A third test (Western blot) was also used with these weak positive samples to resolve any discordant results.
Publication Date: 1989-01-01 PubMed ID: 2562211DOI: 10.1177/104063878900100114Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Antibodies
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Health
- Equine Infectious Anemia
- Horses
- Immunology
- Infectious Disease
- Laboratory Methods
- Serology
- Veterinary Care
- Veterinary Medicine
- Veterinary Research
- Virus
- Western Blot
Summary
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The research explores the effectiveness of two approved diagnostic tests, the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA) in detecting antibodies to equine infectious anemia virus. The study finds a 100% correlation in the results from both tests when applied to 420 sera samples.
Objective and Methodology of the Study
- The study primarily aimed to compare the diagnostic efficacy of two tests – the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA) – in detecting an antibody to the equine infectious anemia virus (EIAV).
- To carry out the comparison, the researchers applied both diagnostic tests to 420 sera samples taken from National Veterinary Services Laboratories check sets.
Results and Findings
- The study found that there was a 100% correlation between the results obtained from both the AGID and competitive ELISA tests. This means that both tests consistently identified the same samples as either positive or negative for EIAV antibodies.
- In addition to testing the sera samples, both the AGID and competitive ELISA tests were used on ‘difficult’ samples with low levels of EIAV antibodies, also known as ‘weak positives’.
- To confirm the results of the AGID and ELISA tests on these weak positive samples, a third test – the Western blot test – was also used.
- The study does not provide specific results for these weak positive samples, but the inclusion of the Western blot test suggests that it was used to resolve any discrepancy in results between the AGID and ELISA tests.
Implication of the Results
- The complete agreement in the results from the two tests provides strong evidence that both the AGID and competitive ELISA tests are reliable diagnostic tools for detecting EIAV antibodies.
- The use of a third confirmatory test in the case of weak positive samples suggests the necessity for additional testing when initial results are unclear.
Cite This Article
APA
Matsushita T, Hesterberg LK, Porter JP, Smith BJ, Newman LE.
(1989).
Comparison of diagnostic tests for the detection of equine infectious anemia antibody.
J Vet Diagn Invest, 1(1), 50-52.
https://doi.org/10.1177/104063878900100114 Publication
Researcher Affiliations
- Syngene Products and Research, Fort Collins, CO 80524.
MeSH Terms
- Animals
- Antibodies, Viral / analysis
- Blotting, Western / veterinary
- Enzyme-Linked Immunosorbent Assay / veterinary
- Immunodiffusion / veterinary
- Infectious Anemia Virus, Equine / immunology
- Predictive Value of Tests
Citations
This article has been cited 5 times.- Nardini R, Autorino GL, Issel CJ, Cook RF, Ricci I, Frontoso R, Rosone F, Scicluna MT. Evaluation of six serological ELISA kits available in Italy as screening tests for equine infectious anaemia surveillance.. BMC Vet Res 2017 Apr 14;13(1):105.
- Issel CJ, Scicluna MT, Cook SJ, Cook RF, Caprioli A, Ricci I, Rosone F, Craigo JK, Montelaro RC, Autorino GL. Challenges and proposed solutions for more accurate serological diagnosis of equine infectious anaemia.. Vet Rec 2013 Feb 23;172(8):210.
- Paré J, Simard C. Comparison of commercial enzyme-linked immunosorbent assays and agar gel immunodiffusion tests for the serodiagnosis of equine infectious anemia.. Can J Vet Res 2004 Oct;68(4):254-8.
- Langemeier JL, Cook SJ, Cook RF, Rushlow KE, Montelaro RC, Issel CJ. Detection of equine infectious anemia viral RNA in plasma samples from recently infected and long-term inapparent carrier animals by PCR.. J Clin Microbiol 1996 Jun;34(6):1481-7.
- Sellon DC. Equine infectious anemia.. Vet Clin North Am Equine Pract 1993 Aug;9(2):321-36.
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