Comparison of histomorphometric characteristics of dorsal colon and pelvic flexure biopsy specimens obtained from horses with large colon volvulus that underwent resection.
Abstract: To determine the degree of histomorphometric damage in dorsal colon and pelvic flexure biopsy specimens (DCBSs and PFBSs, respectively) obtained from horses with large colon volvulus (LCV) and assess the accuracy of predicting short-term outcome for those horses on the basis of DCBS or PFBS characteristics. Methods: 18 horses with ≥ 360° LCV that underwent large colon resection. Methods: During surgery, biopsy specimens from the dorsal colon resection site and the pelvic flexure (when available) were collected from each horse. Interstitial-to-crypt (I:C) ratio (ratio of the lamina propria space occupied by the interstitium to that occupied by crypts), hemorrhage within the lamina propria (mucosal hemorrhage score [MHS] from 0 to 4), and percentage losses of glandular and luminal epithelium were determined in paired biopsy specimens and compared to determine optimal cutoff values for calculating the accuracy of DCBS and PFBS characteristics to predict short-term outcome (survival or nonsurvival after recovery from surgery). Results: Paired biopsy specimens were obtained from 17 of the 18 horses. The I:C ratio and percentage glandular epithelial loss differed between DCBSs and PFBSs. For DCBSs, an I:C ratio ≥ 0.9 and MHS ≥ 3 each predicted patient nonsurvival with 77.8% accuracy. For PFBSs, an I:C ratio ≥ I and MHS ≥ 3 predicted patient nonsurvival with 70.6% and 82.4% accuracy, respectively. Conclusions: Although different, histomorphometric measurements for either DCBSs or PFBSs could be used to accurately predict short-term outcome for horses with LCV that underwent large colon resection, and arguably PFBSs are easier to collect.
Publication Date: 2020-10-28 PubMed ID: 33107753PubMed Central: PMC8972794DOI: 10.2460/ajvr.81.11.899Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study examines the histomorphometric differences in biopsy specimens taken from the dorsal colon and pelvic flexure of horses with large colon volvulus (LCV). The results from the biopsy findings were used to determine the accuracy of predicting short-term outcomes after surgical resection.
Research Methodology
- Horses with a 360-degree LCV that went under large colon resection were the subjects of this study. The sample size totaled up to 18 horses.
- During the surgical process, biopsy specimens were collected from the resection site of the dorsal colon and the pelvic flexure, if available.
- A few parameters were analyzed in both the dorsal colon and pelvic flexure biopsy specimens. These included ratio of the interstitial-to-crypt (I:C), hemorrhage in the lamina propria (mucosal hemorrhage score), and the percentage loss of glandular and luminal epithelium.
Research Findings
- The researchers could obtain paired biopsy specimens from 17 of the 18 horses involved in the study.
- Significant differences were seen in the I:C ratio and the percentage of glandular epithelial loss between the dorsal colon biopsy specimens (DCBSs) and the pelvic flexure biopsy specimens (PFBSs).
- For DCBSs, an I:C ratio equal to or higher than 0.9 and a mucosal hemorrhage score equal to or greater than 3 were able to predict patient non-survival with 77.8% accuracy.
- In the case of PFBSs, an I:C ratio of at least 1 and a mucosal hemorrhage score equal to or higher than 3 predicted patient non-survival with 70.6% and 82.4% accuracy, respectively.
Conclusions
- The histomorphometric metrics calculated from both DCBSs and PFBSs could be effectively employed to predict short-term outcomes for horses with LCV. These horses went through large colon resection post-diagnosis.
- Suggestively, collecting PFBSs is arguably easier, enhancing the prospects of using them for prediction purposes.
Cite This Article
APA
Gonzalez LM, Baker WT, Hughes FE, Blikslager AT, Fogle CA.
(2020).
Comparison of histomorphometric characteristics of dorsal colon and pelvic flexure biopsy specimens obtained from horses with large colon volvulus that underwent resection.
Am J Vet Res, 81(11), 899-903.
https://doi.org/10.2460/ajvr.81.11.899 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Biopsy / veterinary
- Colon / surgery
- Horse Diseases / surgery
- Horses
- Intestinal Volvulus / surgery
- Intestinal Volvulus / veterinary
- Pelvis
Grant Funding
- K01 OD019911 / NIH HHS
- P30 DK034987 / NIDDK NIH HHS
Conflict of Interest Statement
The authors declare that there were no conflicts of interest related to this report.
References
This article includes 12 references
- Driscoll N, Baia P, Fischer AT. Large colon resection and anastomosis in horses: 52 cases (1996–2006). Equine Vet J 2008;40:342–347.
- Ellis CM, Lynch TM, Slone DE. Survival and complications after large colon resection and end-to-end anastomosis for strangulating large colon volvulus in seventy-three horses. Vet Surg 2008;37:786–790.
- Suthers JM, Pinchbeck GL, Proudman CJ. Survival of horses following strangulating large colon volvulus. Equine Vet J 2013;45:219–223.
- Hughes FE, Slone DE Jr. Large colon resection. Vet Clin North Am Equine Pract 1997;13:341–350.
- van Hoogmoed L, Snyder JR, Pascoe JR. Evaluation of uniformity of morphological injury of the large colon following severe colonic torsion. Equine Vet J Suppl 2000;32:98–100.
- Levi O, Affolter VK, Benak J. Use of pelvic flexure biopsy scores to predict short-term survival after large colon volvulus. Vet Surg 2012;41:582–588.
- Gonzalez LM, Fogle CA, Baker WT. Operative factors associated with short-term outcome in horses with large colon volvulus: 47 cases from 2006 to 2013. Equine Vet J 2015;47:279–284.
- Hughes FE, Slone DE. A modified technique for extensive large colon resection and anastomosis in horses. Vet Surg 1998;27:127–131.
- Snyder JR, Olander HJ, Pascoe JR. Morphologic alterations observed during experimental ischemia of the equine large colon. Am J Vet Res 1988;49:801–809.
- van Hoogmoed L, Snyder JR, Pascoe JR. Use of pelvic flexure biopsies to predict survival after large colon torsion in horses. Vet Surg 2000;29:572–577.
- Gonzalez LM, Moeser AJ, Blikslager AT. Animal models of ischemia-reperfusion-induced intestinal injury: progress and promise for translational research. Am J Physiol Gastrointest Liver Physiol 2015;308:G63–G75.
- Hackett ES, Embertson RM, Hopper SA. Duration of disease influences survival to discharge of Thoroughbred mares with surgically treated large colon volvulus. Equine Vet J 2015;47:650–654.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
