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Comparison of histomorphometric characteristics of dorsal colon and pelvic flexure biopsy specimens obtained from horses with large colon volvulus that underwent resection.
Abstract: To determine the degree of histomorphometric damage in dorsal colon and pelvic flexure biopsy specimens (DCBSs and PFBSs, respectively) obtained from horses with large colon volvulus (LCV) and assess the accuracy of predicting short-term outcome for those horses on the basis of DCBS or PFBS characteristics. Methods: 18 horses with ≥ 360° LCV that underwent large colon resection. Methods: During surgery, biopsy specimens from the dorsal colon resection site and the pelvic flexure (when available) were collected from each horse. Interstitial-to-crypt (I:C) ratio (ratio of the lamina propria space occupied by the interstitium to that occupied by crypts), hemorrhage within the lamina propria (mucosal hemorrhage score [MHS] from 0 to 4), and percentage losses of glandular and luminal epithelium were determined in paired biopsy specimens and compared to determine optimal cutoff values for calculating the accuracy of DCBS and PFBS characteristics to predict short-term outcome (survival or nonsurvival after recovery from surgery). Results: Paired biopsy specimens were obtained from 17 of the 18 horses. The I:C ratio and percentage glandular epithelial loss differed between DCBSs and PFBSs. For DCBSs, an I:C ratio ≥ 0.9 and MHS ≥ 3 each predicted patient nonsurvival with 77.8% accuracy. For PFBSs, an I:C ratio ≥ I and MHS ≥ 3 predicted patient nonsurvival with 70.6% and 82.4% accuracy, respectively. Conclusions: Although different, histomorphometric measurements for either DCBSs or PFBSs could be used to accurately predict short-term outcome for horses with LCV that underwent large colon resection, and arguably PFBSs are easier to collect.
Publication Date: 2020-10-28 PubMed ID: 33107753PubMed Central: PMC8972794DOI: 10.2460/ajvr.81.11.899Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This study examines the histomorphometric differences in biopsy specimens taken from the dorsal colon and pelvic flexure of horses with large colon volvulus (LCV). The results from the biopsy findings were used to determine the accuracy of predicting short-term outcomes after surgical resection.
Research Methodology
- Horses with a 360-degree LCV that went under large colon resection were the subjects of this study. The sample size totaled up to 18 horses.
- During the surgical process, biopsy specimens were collected from the resection site of the dorsal colon and the pelvic flexure, if available.
- A few parameters were analyzed in both the dorsal colon and pelvic flexure biopsy specimens. These included ratio of the interstitial-to-crypt (I:C), hemorrhage in the lamina propria (mucosal hemorrhage score), and the percentage loss of glandular and luminal epithelium.
Research Findings
- The researchers could obtain paired biopsy specimens from 17 of the 18 horses involved in the study.
- Significant differences were seen in the I:C ratio and the percentage of glandular epithelial loss between the dorsal colon biopsy specimens (DCBSs) and the pelvic flexure biopsy specimens (PFBSs).
- For DCBSs, an I:C ratio equal to or higher than 0.9 and a mucosal hemorrhage score equal to or greater than 3 were able to predict patient non-survival with 77.8% accuracy.
- In the case of PFBSs, an I:C ratio of at least 1 and a mucosal hemorrhage score equal to or higher than 3 predicted patient non-survival with 70.6% and 82.4% accuracy, respectively.
Conclusions
- The histomorphometric metrics calculated from both DCBSs and PFBSs could be effectively employed to predict short-term outcomes for horses with LCV. These horses went through large colon resection post-diagnosis.
- Suggestively, collecting PFBSs is arguably easier, enhancing the prospects of using them for prediction purposes.
Cite This Article
APA
Gonzalez LM, Baker WT, Hughes FE, Blikslager AT, Fogle CA.
(2020).
Comparison of histomorphometric characteristics of dorsal colon and pelvic flexure biopsy specimens obtained from horses with large colon volvulus that underwent resection.
Am J Vet Res, 81(11), 899-903.
https://doi.org/10.2460/ajvr.81.11.899 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Biopsy / veterinary
- Colon / surgery
- Horse Diseases / surgery
- Horses
- Intestinal Volvulus / surgery
- Intestinal Volvulus / veterinary
- Pelvis
Grant Funding
- K01 OD019911 / NIH HHS
- P30 DK034987 / NIDDK NIH HHS
Conflict of Interest Statement
The authors declare that there were no conflicts of interest related to this report.
References
This article includes 12 references
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