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Complete Genome Sequences of Four African Horse Sickness Virus Strains from a Commercial Tetravalent Live Attenuated Vaccine.
Abstract: This is a report of the complete genome sequences of plaque-selected isolates of each of the four virus strains included in a South African commercial tetravalent African horse sickness attenuated live virus vaccine.
Copyright © 2015 Guthrie et al.
Publication Date: 2015-11-25 PubMed ID: 26607890PubMed Central: PMC4661309DOI: 10.1128/genomeA.01375-15Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- African Horse Sickness
- Animal Science
- Animal Studies
- Comparative Study
- Disease control
- Disease Diagnosis
- Disease Management
- Disease Treatment
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Science
- Genetic Sequencing
- Genomics
- Infectious Disease
- Vaccine development
- Veterinary Medicine
- Veterinary Research
- Virology
- Virus
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article presents the full genetic sequences of four strains of African Horse Sickness Virus that are used in a commercial vaccine for the disease in South Africa.
Overview of the Research
The main objective of this study was to sequence the genomes of the four virus strains used in a tetravalent (designed to protect against four different strains) live attenuated (weakened) vaccine for African Horse Sickness. The strains were selected through plaque assay, a method that involves isolating and growing individual viruses in cells.
Methodology of the Study
- The researchers isolated four different strains of the African Horse Sickness Virus, which are included in a South African commercially produced vaccine against the disease.
- These strains were plaque-selected, implying they were singled out in lab culture plates for their ability to infect cells and form visible patches or plaques. This procedure helps in isolating individual strains from a mixture.
- After isolation, the genetic material of the viruses, their genetic blueprint was completely sequenced. This sequencing helps in understanding the genetic composition of these virus strains, including the genes that cause disease and those that evoke immune response.
- The complete genome sequences serve as references for future research, to understand the genetic diversity of the virus and to develop better, possibly more specific, vaccines against each strain.
Significance of the Research
- Providing the complete genome sequence of the four African Horse Sickness Virus strains can aid in understanding the diseases they cause.
- The information is valuable for the development of diagnostic tests and efficient vaccines.
- The sequenced genomes can act as valued references for future epidemiology studies, virus tracking and understanding the genetic diversity of the virus.
This research contributes to the fields of virology, epidemiology and vaccine development by providing a detailed understanding of the genetic composition of the four African Horse Sickness Virus strains. Understanding these genomes helps in tracking the spread of these virus strains, predicting and controlling outbreaks and improving current vaccinations.
Cite This Article
APA
Guthrie AJ, Coetzee P, Martin DP, Lourens CW, Venter EH, Weyer CT, Joone C, le Grange M, Harper CK, Howell PG, MacLachlan NJ.
(2015).
Complete Genome Sequences of Four African Horse Sickness Virus Strains from a Commercial Tetravalent Live Attenuated Vaccine.
Genome Announc, 3(6).
https://doi.org/10.1128/genomeA.01375-15 Publication
Researcher Affiliations
- Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa alan.guthrie@up.ac.za.
- Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Department of Integrative Biomedical Sciences, Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Rosebank, Cape Town, South Africa.
- Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Veterinary Genetics Laboratory, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
- Equine Viral Diseases Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, California, USA Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
References
This article includes 7 references
- Zwart L, Potgieter CA, Clift SJ, Van Staden V. Characterising non-structural protein NS4 of African horse sickness virus. PLoS One 2015 10:e0124281.
- Von Teichman BF, Dungu B, Smit TK. In vivo cross-protection to African horse sickness serotypes 5 and 9 after vaccination with serotypes 8 and 6. Vaccine 2010 28:6505–6517.
- Howell PG, Kümm NA, Botha MJ. The application of improved techniques to the identification of strains of bluetongue virus. Onderstepoort J Vet Res 1970 37:59–66.
- Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, van Dijk AA. Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 2009 90:1423–1432.
- Guthrie AJ, Coetzee P, Martin DP, Lourens CW, Venter EH, Weyer CT, Joone C, le Grange M, Harper CK, Howell PG, MacLachlan NJ. Complete genome sequences of the three African horse sickness virus strains from a commercial trivalent live attenuated vaccine. Genome Announc 2015 3(4):e00814-15.
- Manole V, Laurinmäki P, Van Wyngaardt W, Potgieter CA, Wright IM, Venter GJ, van Dijk AA, Sewell BT, Butcher SJ. Structural insight into African horsesickness virus infection. J Virol 2012 86:7858–7866.
- Martin DP, Murrell B, Golden M, Khoosal A, Muhire B. RDP4: detection and analysis of recombination patterns in virus genomes. Virol Evol 2015 1:vev003.
Citations
This article has been cited 4 times.- Zhang M, Wang XF, Guo SF, Wang L, Fu BF, Wang JW, Song YF, Yang XY, Hao SY, Zhang QY, Zhang B, Yang CH. Identification and Genetic Characterization of a Strain of African Horse Sickness Virus Serotype 1 and Its Safety Evaluation in a Mouse Model. Microorganisms 2025 Oct 6;13(10).
- Ngoveni HG, van Schalkwyk A, Koekemoer JJO. Evidence of Intragenic Recombination in African Horse Sickness Virus. Viruses 2019 Jul 18;11(7).
- Schade-Weskott ML, van Schalkwyk A, Koekemoer JJO. A correlation between capsid protein VP2 and the plaque morphology of African horse sickness virus in cell culture. Virus Genes 2018 Aug;54(4):527-535.
- Weyer CT, Grewar JD, Burger P, Rossouw E, Lourens C, Joone C, le Grange M, Coetzee P, Venter E, Martin DP, MacLachlan NJ, Guthrie AJ. African Horse Sickness Caused by Genome Reassortment and Reversion to Virulence of Live, Attenuated Vaccine Viruses, South Africa, 2004-2014. Emerg Infect Dis 2016 Dec;22(12):2087-2096.
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