Complete genome sequences of toxigenic Clostridioides difficile isolated from Australian feral horses.
Abstract: Recent increases in community-associated Clostridioides difficile infections have highlighted the importance of monitoring toxigenic C. difficile from animal and environmental sources. We provide the complete circularized genomes of two toxigenic C. difficile strains isolated from feral horse faeces. Genome N64 (sequence type 964) consists of a single chromosome of 4,078,791 bp, while genome H251 (sequence type 963) comprises one chromosome (4,304,722 bp) and three plasmids (150,942 bp, 11,534 bp, and 9,074 bp).
Publication Date: 2024-11-22 PubMed ID: 39576097PubMed Central: PMC11636274DOI: 10.1128/mra.01086-24Google Scholar: Lookup
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Summary
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This research article presents the complete genome sequences of two harmful bacterial strains collected from the feces of wild horses in Australia.
Objective of the Research
The research aims to provide comprehensive genomic findings of two toxigenic strains of the Clostridioides difficile bacterium which were isolated from the feces of feral horses in Australia. These findings contribute to the body of knowledge needed to monitor these infections, especially considering the recent increase in people falling ill due to community-associated infections.
Method of the Research
- The researchers isolated two strains of the Clostridioides difficile bacteria from the excreta of wild horses.
- They provided the complete circular genome of these bacterial strains, pointing out that robust genomic information is essential to study pathogens’ genetic features and pathogenic mechanisms.
- Genome sequencing techniques were employed to unravel the genetic makeup of the two bacterial strains, referred to as Genome N64 and Genome H251 in the study.
Findings from the Research
- Genome N64, categorized as sequence type 964, consists of a single chromosome of 4,078,791 base pairs (bp). This numerical representation provides specifics about the total genetic content of this bacterial strain.
- On the other hand, Genome H251, identified as sequence type 963, is composed of one chromosome made up of 4,304,722 base pairs (bp) and three plasmids — these are miniature genetic structures that are separate from the bacterial chromosome. The sizes of these plasmids are 150,942 bp, 11,534 bp, and 9,074 bp.
- Understanding the genomic characteristics of the bacterial strains provides valuable information about their pathogenic capabilities and their overall role in disease transmission within the community.
Cite This Article
APA
Hain-Saunders N, Knight DR, Bruce M, Riley TV.
(2024).
Complete genome sequences of toxigenic Clostridioides difficile isolated from Australian feral horses.
Microbiol Resour Announc, 13(12), e0108624.
https://doi.org/10.1128/mra.01086-24 Publication
Researcher Affiliations
- 1Centre for Biosecurity and One Health Harry Butler Institute, Murdoch University, Murdoch, Western Australia, Australia.
- School of Biomedical Sciences, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia.
- Department of Microbiology, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia.
- 1Centre for Biosecurity and One Health Harry Butler Institute, Murdoch University, Murdoch, Western Australia, Australia.
- School of Biomedical Sciences, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia.
- Department of Microbiology, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia.
- School of Medical and Health Sciences, Edith Cowan University, Joondalup, Western Australia, Australia.
Grant Funding
- Research and Innovation Strategic Scholarship / Australian Government (Federal Government)
Conflict of Interest Statement
The authors declare no conflict of interest.
References
This article includes 9 references
- Australian commission on safety and quality in health care. Clostridioides difficile infection data snapshot report: 2020 and 2021. 2023.
- Feuerstadt P, Theriault N, Tillotson G. The burden of CDI in the United States: a multifactorial challenge. BMC Infect Dis 23:132.
- Harvey AM, Meggiolaro MN, Hall E, Watts ET, Ramp D, Šlapeta J. Wild horse populations in south-east Australia have a high prevalence of Strongylus vulgaris and may act as a reservoir of infection for domestic horses. Int J Parasitol Parasites Wildl 8:156–163.
- Hain-Saunders NMR, Knight DR, Bruce M, Byrne D, Riley TV. Genomic analysis of Clostridioides difficile recovered from horses in Western Australia. Microorganisms 11:1743.
- Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 13:e1005595.
- Wick RR, Schultz MB, Zobel J, Holt KE. Bandage: interactive visualization of de novo genome assemblies. Bioinformatics 31:3350–3352.
- Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, Lomsadze A, Ostell J. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 44:6614–6624.
- Griffiths D, Fawley W, Kachrimanidou M, Bowden R, Crook DW, Fung R, Golubchik T, Harding RM, Jeffery KJM, Jolley KA, Kirton R, Peto TE, Rees G, Stoesser N, Vaughan A, Walker AS, Young BC, Wilcox M, Dingle KE. Multilocus sequence typing of Clostridium difficile. J Clin Microbiol 48:770–778.
- Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y, Wishart DS. PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res 44:W16–21.
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