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Equine veterinary journal2023; doi: 10.1111/evj.13960

Composition, architecture and biomechanical properties of articular cartilage in differently loaded areas of the equine stifle.

Abstract: Strategies for articular cartilage repair need to take into account topographical differences in tissue composition and architecture to achieve durable functional outcome. These have not yet been investigated in the equine stifle. Objective: To analyse the biochemical composition and architecture of three differently loaded areas of the equine stifle. We hypothesise that site differences correlate with the biomechanical characteristics of the cartilage. Methods: Ex vivo study. Methods: Thirty osteochondral plugs per location were harvested from the lateral trochlear ridge (LTR), the distal intertrochlear groove (DITG) and the medial femoral condyle (MFC). These underwent biochemical, biomechanical and structural analysis. A linear mixed model with location as a fixed factor and horse as a random factor was applied, followed by pair-wise comparisons of estimated means with false discovery rate correction, to test for differences between locations. Correlations between biochemical and biomechanical parameters were tested using Spearman's correlation coefficient. Results: Glycosaminoglycan content was different between all sites (estimated mean [95% confidence interval (CI)] for LTR 75.4 [64.5, 88.2], for intercondylar notch (ICN) 37.3 [31.9, 43.6], for MFC 93.7 [80.1109.6] μg/mg dry weight), as were equilibrium modulus (LTR2.20 [1.96, 2.46], ICN0.48 [0.37, 0.6], MFC1.36 [1.17, 1.56] MPa), dynamic modulus (LTR7.33 [6.54, 8.17], ICN4.38 [3.77, 5.03], MFC5.62 [4.93, 6.36] MPa) and viscosity (LTR7.49 [6.76, 8.26], ICN16.99 [15.88, 18.14], MFC8.7 [7.91,9.5]°). The two weightbearing areas (LTR and MCF) and the non-weightbearing area (ICN) differed in collagen content (LTR 139 [127, 152], ICN176[162, 191], MFC 127[115, 139] μg/mg dry weight), parallelism index and angle of collagen fibres. The strongest correlations were between proteoglycan content and equilibrium modulus (r: 0.642; p: 0.001), dynamic modulus (r: 0.554; p < 0.001) and phase shift (r: -0.675; p < 0.001), and between collagen orientation angle and equilibrium modulus (r: -0.612; p < 0.001), dynamic modulus (r: -0.424; p < 0.001) and phase shift (r: 0.609; p < 0.001). Conclusions: Only a single sample per location was analysed. Conclusions: There were significant differences in cartilage biochemical composition, biomechanics and architecture between the three differently loaded sites. The biochemical and structural composition correlated with the mechanical characteristics. These differences need to be acknowledged by designing cartilage repair strategies. Unassigned: Les stratégies de réparation du cartilage articulaire doivent tenir compte des différences topographiques en ce qui a trait à la composition et l'architecture des tissues, afin d'obtenir un résultat durable et fonctionnel. Celles-ci n'ont pas encore été étudiées chez le grasset équin. Objective: Analyser la composition biochimique et l'architecture de trois régions du grasset portant une quantité de poids différente. Nous émettons l'hypothèse que les différences entre régions seront corrélées aux caractéristiques biomécaniques du cartilage. TYPE D'ÉTUDE: Étude ex vivo. MÉTHODES: Trente échantillons ostéochondraux par site ont été récoltés à partir de la lèvre latérale de la trochlée fémorale (LTR), le sillon intertrochléaire distal (DITG) et le condyle fémoral médial (MFC). Ceux-ci ont été soumis à des tests biochimiques, biomécaniques et une analyse structurelle. Un modèle linéaire mixte avec localisation comme facteur fixe et cheval comme facteur randomisé a été appliqué. Puis, ont suivi des comparaisons par paires de moyennes estimées avec contrôle du taux de fausses découvertes, pour tester les différences entre les divers sites. Les corrélations entre les paramètres biochimiques et biomécaniques ont été testé par le coefficient de corrélation Spearman. RÉSULTATS: Le contenu en glycosaminoglycans était différent à chacun des sites (moyenne estimée [95% CI] pour LTR 75.4 [64.5, 88.2], pour ICN 37.3 [31.9, 43.6], pour MFC 93.7[80.1109.6]μg/mg matière sèche), tout comme le module d'équilibre (LTR2.20 [1.96, 2.46], ICN0.48 [0.37, 0.6], MFC1.36 [1.17, 1.56] MPa), le module dynamique (LTR7.33 [6.54, 8.17], ICN4.38[3.77, 5.03], MFC5.62[4.93, 6.36] MPa) et la viscosité (LTR7.49[6.76, 8.26], ICN16.99 [15.88, 18.14], MFC8.7 [7.91, 9.5]°). Les deux régions portant du poids (LTR et MFC) et la région ne supportant pas de poids (ICN) diffèrent par rapport à leur contenu en collagène (LTR 139 [127152], ICN176 [162191], MFC 127 [115139] μg/mg matière sèche), à l'index de parallélisle et à l'angle des fibres de collagène. Les corrélations les plus fortes étaient entre le contenu en protéoglycans et le module d'équilibre (r: 0.642; p: 0.001), le module dynamique (r: 0.554; p < 0.001) et le changement de phase (r:−0.675; p < 0.001), et entre l'angle d'orientation du collagène et le module d'équilibre (r:−0.612; p < 0.001), le module dynamique (r:−0.424; p < 0.001) et le changement de phase (r: 0.609;p:<0.001). Unassigned: Seulement un échantillon par site a été soumis aux analyses. Conclusions: Il existe des différences significatives dans la composition biochimique, biomécanique et l'architecture du cartilage entre les trois sites échantillonnés. La composition biochimique et structurelle corrèle avec les caractéristiques mécaniques. Ces différences doivent être prises en compte lors de la création de stratégies de réparation du cartilage.
© 2023 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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Publication Date: 2023-06-27 PubMed ID: 37376723DOI: 10.1111/evj.13960Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the biochemical composition and structure of cartilage in different areas of the equine stifle (a joint in the horse’s hind leg), and how these factors correspond with the mechanical characteristics of the cartilage depending on the weight load. Findings reveal significant variations in the cartilage’s makeup, biomechanics, and architecture across the various sites that bear differing weight. These findings are crucial for determining the most effective strategies for cartilage repair.

Methodology

  • The study used an ex vivo approach, studying tissues outside of an organism.
  • Three areas from the horse’s stifle were selected due to their differences in weight loading: the lateral trochlear ridge (LTR), the distal intertrochlear groove (DITG), and the medial femoral condyle (MFC).
  • From each of these locations, 30 osteochondral plugs (samples that contain both bone and cartilage) were collected and subjected to biomechanical, biochemical, and structural analyses.
  • A statistical linear mixed model method was used to test for differences between the sites, looking closely at the location and individual differences between horses.
  • The researchers also used the Spearman correlation coefficient to understand any correlations between the biochemical and biomechanical factors studied.

Key Findings

  • The content of glycosaminoglycan, a vital component of cartilage, varied across the different sites.
  • Equilibrium modulus, dynamic modulus and viscosity, all mechanical properties of the cartilage, varied between the sites as well.
  • The content of collagen, another crucial component of cartilage, as well as the parallelism index and angle of collagen fibers were found to be different in the two weight-bearing areas (LTR and MFC) and in the non-weight-bearing area (ICN).
  • Notably, the strongest correlations observed were between proteoglycan content (a type of glycosaminoglycan) and various mechanical characteristics such as the equilibrium modulus, dynamic modulus, and phase shift.
  • Similarly, the orientation angle of collagen in the cartilage also showed strong correlations with the aforementioned mechanical characteristics.

Conclusions and Implications

  • The study found considerable differences in the biochemistry, biomechanics, and structure of cartilage between the three different sites in the equine stifle.
  • The biochemical and structural properties of the cartilage were closely linked to its biomechanical characteristics.
  • These findings are critical for designing effective cartilage repair strategies, given the need to consider the site-specific properties of the cartilage.

Cite This Article

APA
Fugazzola M, Nissinen MT, Jäntti J, Tuppurainen J, Plomp S, Te Moller N, Mäkelä JTA, van Weeren R. (2023). Composition, architecture and biomechanical properties of articular cartilage in differently loaded areas of the equine stifle. Equine Vet J. https://doi.org/10.1111/evj.13960

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English

Researcher Affiliations

Fugazzola, Maria
  • Department of Equine Sciences, Utrecht University, Utrecht, The Netherlands.
Nissinen, Mikko T
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
Jäntti, Jiri
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
  • Clinical Physiology and Nuclear Medicine, Kuopio University Hospital, Kuopio, Finland.
Tuppurainen, Juuso
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
Plomp, Saskia
  • Department of Equine Sciences, Utrecht University, Utrecht, The Netherlands.
Te Moller, Nikae
  • Department of Equine Sciences, Utrecht University, Utrecht, The Netherlands.
Mäkelä, Janne T A
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
  • Clinical Physiology and Nuclear Medicine, Kuopio University Hospital, Kuopio, Finland.
van Weeren, Rene
  • Department of Equine Sciences, Utrecht University, Utrecht, The Netherlands.

Grant Funding

  • 307932 / Instrumentarium Science Foundation, Academy of Finland
  • 5041795 / Kuopio University Hospital

References

This article includes 43 references
  1. Van Turnhout MC, Schipper H, Engel B, Buist W, Kranenbarg S, Van Leeuwen JL. Postnatal development of collagen structure in ovine articular cartilage.. BMC Dev Biol 2010;10:60.
    doi: 10.1186/1471-213x-10-62google scholar: lookup
  2. Van Turnhout MC, Kranenbarg S, Van Leeuwen JL. Contribution of postnatal collagen reorientation to depth-dependent mechanical properties of articular cartilage.. Biomech Model Mechanobiol 2011;10(2):269-79.
    doi: 10.1007/s10237-010-0233-7google scholar: lookup
  3. Julkunen P, Halmesmäki EP, Iivarinen J, Rieppo L, Närhi T, Marjanen J. Effects of growth and exercise on composition, structural maturation and appearance of osteoarthritis in articular cartilage of hamsters.. J Anat 2010;217(3):262-74.
    doi: 10.1111/j.1469-7580.2010.01270.xgoogle scholar: lookup
  4. Brama PAJ, Tekoppele JM, Bank RA, Barneveld A, van Weeren PR. Development of biochemical heterogeneity of articular cartilage: influences of age and exercise.. Equine Vet J 2002;34(3):265-9.
    doi: 10.2746/042516402776186146google scholar: lookup
  5. Brama PAJ, Tekoppele JM, Bank RA, Karssenberg D, Barneveld A, van Weeren PR. Topographical mapping of biochemical properties of articular cartilage in the equine fetlock joint.. Equine Vet J 2000;32(1):19-26.
    doi: 10.2746/042516400777612062google scholar: lookup
  6. Oinas J, Ronkainen AP, Rieppo L, Finnilä MAJ, Iivarinen JT, van Weeren PR. Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.. Sci Rep 2018;8(1):11357.
    doi: 10.1038/s41598-018-29655-5google scholar: lookup
  7. Hyttinen MM, Holopainen J, van Weeren PR, Firth EC, Helminen HJ, Brama PAJ. Changes in collagen fibril network organization and proteoglycan distribution in equine articular cartilage during maturation and growth.. J Anat 2009;215(5):584-91.
    doi: 10.1111/j.1469-7580.2009.01140.xgoogle scholar: lookup
  8. Julkunen P, Harjula T, Iivarinen J, Marjanen J, Seppänen K, Närhi T. Biomechanical, biochemical and structural correlations in immature and mature rabbit articular cartilage.. Osteoarthr Cartil 2009;17(12):1628-38.
    doi: 10.1016/j.joca.2009.07.002google scholar: lookup
  9. Changoor A, Hurtig MB, Runciman RJ, Quesnel AJ, Dickey JP, Lowerison M. Mapping of donor and recipient site properties for osteochondral graft reconstruction of subchondral cystic lesions in the equine stifle joint.. Equine Vet J 2006;38(4):330-6.
    doi: 10.2746/042516406777749254google scholar: lookup
  10. White JL, Salinas EY, Link JM, Hu JC, Athanasiou KA. Characterization of adult and neonatal articular cartilage from the equine stifle.. J Equine Vet Sci 2021;96:103294.
    doi: 10.1016/j.jevs.2020.103294google scholar: lookup
  11. Mäkelä JTA, Rezaeian ZS, Mikkonen S, Madden R, Han S-K, Jurvelin JS. Site-dependent changes in structure and function of lapine articular cartilage 4 weeks after anterior cruciate ligament transection.. Osteoarthr Cartil 2014;22(6):869-78.
    doi: 10.1016/j.joca.2014.04.010google scholar: lookup
  12. Mäkelä JTA, Huttu MRJ, Korhonen RK. Structure-function relationships in osteoarthritic human hip joint articular cartilage.. Osteoarthr Cartil 2012;20(11):1268-77.
    doi: 10.1016/j.joca.2012.07.016google scholar: lookup
  13. Fugazzola MC, van Weeren PR. Surgical osteochondral defect repair in the horse-a matter of form or function?. Equine Vet J 2020;52(4):489-99.
    doi: 10.1111/evj.13231google scholar: lookup
  14. Barrett M, Frisbie D. Stifle.. 2016. p. 385.
  15. Fowlie J G, Stick J, Nickels F. The stifle.. 2012. p. 1435-6.
  16. Bodó G, Vásárhelyi G, Hangody L, Módis L. Mosaic arthroplasty of the medial femoral condyle in horses - an experimental study.. Acta Vet Hung 2013;62(2):155-68.
    doi: 10.1556/avet.2013.059google scholar: lookup
  17. Martín AR, Patel JM, Zlotnick HM, Carey JL, Mauck RL. Emerging therapies for cartilage regeneration in currently excluded ‘red knee’ populations.. NPJ Reg Med 2019;4(1):1-11.
    doi: 10.1038/s41536-019-0074-7google scholar: lookup
  18. Medvedeva EV, Grebenik EA, Gornostaeva SN, Telpuhov VI, Lychagin AV, Timashev PS. Repair of damaged articular cartilage: current approaches and future directions.. Int J Mol Sci 2018;19(8):2366.
    doi: 10.3390/ijms19082366google scholar: lookup
  19. Ristle R. Sample size Calculator Version 1.0541.. 2021.
  20. Brama PAJ, Holopainen J, van Weeren PR, Firth EC, Helminen HJ, Hyttinen MM. Effect of loading on the organization of the collagen fibril network in juvenile equine articular cartilage.. J Orthop Res 2009;27(9):1226-34.
    doi: 10.1002/jor.20866google scholar: lookup
  21. Halley SE, Bey MJ, Haladik JA, Lavagnino M, Arnoczky SP. Three dimensional, radiosteriometric analysis (RSA) of equine stifle kinematics and articular surface contact: a cadaveric study.. Equine Vet J 2014;46(3):364-9.
    doi: 10.1111/evj.12127google scholar: lookup
  22. Fowlie JG, Arnoczky SP, Stick JA, Pease AP. Meniscal translocation and deformation throughout the range of motion of the equine stifle joint: an in vitro cadaveric study.. Equine Vet J 2011;43(3):259-64.
    doi: 10.1111/j.2042-3306.2010.00291.xgoogle scholar: lookup
  23. Huttu MRJ, Puhakka J, Mäkelä JTA, Takakubo Y, Tiitu V, Saarakkala S. Cell-tissue interactions in osteoarthritic human hip joint articular cartilage.. Connect Tissue Res 2014;55(4):282-91.
    doi: 10.3109/03008207.2014.912645google scholar: lookup
  24. Hayes WC, Keer LM, Herrmann G, Mockros LF. A mathematical analysis for indentation tests of articular cartilage.. J Biomech 1972;5(5):541-51.
    doi: 10.1016/0021-9290(72)90010-3google scholar: lookup
  25. Kiviranta I, Jurvelin J, Säämänen AM, Helminen HJ. Microspectrophotometric quantitation of glycosaminoglycans in articular cartilage sections stained with safranin O.. Histochemistry 1985;82(3):249-55.
    doi: 10.1007/bf00501401google scholar: lookup
  26. Boskey A, Pleshko CN. FT-IR imaging of native and tissue-engineered bone and cartilage.. Biomaterials 2007;28(15):2465-78.
    doi: 10.1016/j.biomaterials.2006.11.043google scholar: lookup
  27. Camacho N, West P, Torzilli P, Mendelsohn R. FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage.. Biopolymers 2001;62:1-8.
    doi: 10.1002/1097-0282(2001)62:1<1::aid-bip10>3.0.co;2-ogoogle scholar: lookup
  28. Rieppo J, Hyttinen MM, Halmesmaki E, Ruotsalainen H, Vasara A, Kiviranta I. Changes in spatial collagen content and collagen network architecture in porcine articular cartilage during growth and maturation.. Osteoarthr Cartil 2009;17(4):448-55.
    doi: 10.1016/j.joca.2008.09.004google scholar: lookup
  29. Mansour JM. Biomechanics of cartilage.. 2013. p. 69-83.
  30. Murray RC, Birch HL, Lakhani K, Goodship AE. Biochemical composition of equine carpal articular cartilage is influenced by short-term exercise in a site-specific manner.. Osteoarthr Cartil 2001;9(7):625-32.
    doi: 10.1053/joca.2001.0462google scholar: lookup
  31. Brama PA, Tekoppele JM, Bank RA, van Weeren PR, Barneveld A. Influence of different exercise levels and age on the biochemical characteristics of immature equine articular cartilage.. Equine Vet J 1999;31(S31):55-61.
    doi: 10.1111/j.2042-3306.1999.tb05314.xgoogle scholar: lookup
  32. van Weeren PR, Firth EC, Brommer H, Hyttinen MM, Helminen AE, Rogers CW. Early exercise advances the maturation of glycosaminoglycans and collagen in the extracellular matrix of articular cartilage in the horse.. Equine Vet J 2008;40(2):128-35.
    doi: 10.2746/042516408x253091google scholar: lookup
  33. Cornelissen BP, van Weeren PR, Ederveen AG, Barneveld A. Influence of exercise on bone mineral density of immature cortical and trabecular bone of the equine metacarpus and proximal sesamoid bone.. Equine Vet J 1999;31(S31):79-85.
    doi: 10.1111/j.2042-3306.1999.tb05318.xgoogle scholar: lookup
  34. Stover SM, Pool RR, Martin RB, Morgan JP. Histological features of the dorsal cortex of the third metacarpal bone mid-diaphysis during postnatal growth in thoroughbred horses.. J Anat 1992;181(Pt 3):455.
  35. Cherdchutham W, Becker C, Smith RK, Barneveld A, van Weeren PR. Age-related changes and effect of exercise on the molecular composition of immature equine superficial digital flexor tendons.. Equine Vet J 1999;31(S31):86-94.
    doi: 10.1111/j.2042-3306.1999.tb05319.xgoogle scholar: lookup
  36. Ebrahimi M, Turunen MJ, Finnilä MA, Joukainen A, Kröger H, Saarakkala S. Structure-function relationships of healthy and osteoarthritic human Tibial cartilage: experimental and numerical investigation.. Ann Biomed Eng 2020;48(12):2887-900.
    doi: 10.1007/s10439-020-02559-0google scholar: lookup
  37. Broom ND. Abnormal softening in articular cartilage. Its relationship to the collagen framework.. Arthritis Rheum 1982;25(10):1209-16.
    doi: 10.1002/art.1780251010google scholar: lookup
  38. Eschweiler J, Horn N, Rath B, Betsch M, Baroncini A, Tingart M. The biomechanics of cartilage-an overview.. Life 2021;11(4):1647-57.
    doi: 10.3390/life11040302google scholar: lookup
  39. Nelson BB, Mäkelä JTA, Lawson TB, Patwa AN, Barrett MF, McIlwraith CW. Evaluation of equine articular cartilage degeneration after mechanical impact injury using cationic contrast-enhanced computed tomography.. Osteoarthr Cartil 2019;27(8):1219-28.
    doi: 10.1016/j.joca.2019.04.015google scholar: lookup
  40. Nelson BB, Makela JTA, Lawson TB, Patwa AN, Snyder BD, McIlwraith CW. Cationic contrast-enhanced computed tomography distinguishes between reparative, degenerative, and healthy equine articular cartilage.. J Orthop Res 2020;39(8):1647-57.
    doi: 10.1002/jor.24894google scholar: lookup
  41. Nakagawa Y, Mukai S, Yabumoto H, Tarumi E, Nakamura T. Serial changes of the cartilage in recipient sites and their Mirror sites on second-look imaging after Mosaicplasty.. Am J Sports Med 2016;44(5):1243-8.
    doi: 10.1177/0363546516634299google scholar: lookup
  42. De Caro F, Bisicchia S, Amendola A, Ding L. Large fresh osteochondral allografts of the knee: a systematic clinical and basic science review of the literature.. Arthroscopy 2015;31(4):757-65.
    doi: 10.1016/j.arthro.2014.11.025google scholar: lookup
  43. Sherman SL, Garrity J, Bauer K, Cook J, Stannard J, Bugbee W. Fresh osteochondral allograft transplantation for the knee: current concepts.. J Am Acad Orthopaed Surg 2014;22(2):121-33.
    doi: 10.5435/jaaos-22-02-121google scholar: lookup

Citations

This article has been cited 5 times.
  1. Jäntti J, Tuppurainen J, Joenathan A, Leskinen HPP, Cagnoni AM, Mertano H, Poimala M, Nippolainen E, Afara IO, Honkanen JTJ, Matikka H, Töyräs J, Snyder BD, Stok KS, Nelson BB, Grinstaff MW, Mäkelä JTA. Ultrasmall Surface-Charge-Modified Tantalum Oxide Nanoparticles for the Assessment of Articular Cartilage Using Contrast-Enhanced Computed Tomography. ACS Nano 2026 Jan 27;20(3):2717-2729.
    doi: 10.1021/acsnano.5c15722pubmed: 41534877google scholar: lookup
  2. Leskinen HPP, Tuppurainen J, Jäntti J, Mäkelä JTA, Nippolainen E, Afara IO, Töyräs J, Nykänen O, Nissi MJ. Orientation-Independent T2 Mapping Enhances MRI-Based Cartilage Characterization. Ann Biomed Eng 2025 Sep;53(9):2120-2130.
    doi: 10.1007/s10439-025-03774-3pubmed: 40528094google scholar: lookup
  3. Paakkari P, Inkinen SI, Jäntti J, Tuppurainen J, Fugazzola MC, Joenathan A, Ylisiurua S, Nieminen MT, Kröger H, Mikkonen S, van Weeren R, Snyder BD, Töyräs J, Honkanen MKM, Matikka H, Grinstaff MW, Honkanen JTJ, Mäkelä JTA. Dual-Contrast Agent with Nanoparticle and Molecular Components in Photon-Counting Computed Tomography: Assessing Articular Cartilage Health. Ann Biomed Eng 2025 Jun;53(6):1423-1438.
    doi: 10.1007/s10439-025-03715-0pubmed: 40155520google scholar: lookup
  4. Tuppurainen J, Paakkari P, Jäntti J, Nissinen MT, Fugazzola MC, van Weeren R, Ylisiurua S, Nieminen MT, Kröger H, Snyder BD, Joenathan A, Grinstaff MW, Matikka H, Korhonen RK, Mäkelä JTA. Revealing Detailed Cartilage Function Through Nanoparticle Diffusion Imaging: A Computed Tomography & Finite Element Study. Ann Biomed Eng 2024 Sep;52(9):2584-2595.
    doi: 10.1007/s10439-024-03552-7pubmed: 39012563google scholar: lookup
  5. Księżarczyk MM, IJsseldijk LL, van Weeren PR, Levato R, Malda J. Age-dependent development and microarchitecture of the osteochondral unit of the humeral head in harbour porpoises (Phocoena phocoena). Sci Rep 2026 Feb 12;16(1).
    doi: 10.1038/s41598-026-39726-7pubmed: 41673214google scholar: lookup
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