Concentrations of substance P and prostaglandin E2 in synovial fluid of normal and abnormal joints of horses.
Abstract: To correlate substance P content of synovial fluid with prostaglandin E2 content, radiographic evidence of joint abnormality, and anatomic location of the joint for normal and osteoarthritic joints of horses. Methods: Synovial fluid from 46 normal joints in 21 horses and 16 osteoarthritic joints in 10 horses. Methods: Normal and osteoarthritic joints were identified by clinical and radiographic examination, by response to nerve blocks, during scintigraphy or surgery, or by clinicopathologic evaluation. Substance P and prostaglandin E2 contents of synovial fluid were determined by radioimmunoassay. Radio-graphs of joints were assigned a numeric score reflecting severity of lesions. Joints were assigned a numeric score reflecting anatomic location. Results: Median concentrations of substance P and prostaglandin E2 were significantly increased in osteoarthritic joints, compared with normal joints. A significant correlation was found between concentrations of substance P and prostaglandin E2 in synovial fluid, but a correlation was not detected between substance P concentration in synovial fluid and anatomic location of the joint or between radiographic scores of osteoarthritic joints and concentrations of substance P or prostaglandin E2. Conclusions: A correlation existed between concentrations of substance P and prostaglandin E2 in synovial fluid obtained from normal and osteoarthritic joints. However, content of substance P in synovial fluid cannot be predicted by the radiographic appearance of the joint or its anatomic location. Substance P and prostaglandin E2 may share an important and related role in the etiopathogenesis of osteoarthritis, lending credence to the importance of neurogenic inflammation in horses.
Publication Date: 2000-06-13 PubMed ID: 10850851DOI: 10.2460/ajvr.2000.61.714Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the relationship between the presence of substance P and prostaglandin E2 in the synovial fluid of horse joints, their role in osteoarthritis development and how their concentration levels relate to joint abnormality and anatomical location.
Research Methodology
- The study involved synovial fluid collected from 46 normal and 16 osteoarthritic horse joints.
- A combination of clinical and radiographic examinations, nerve block responses, scintigraphy or surgery, and clinicopathologic evaluations were used to identify and categorize the normal and osteoarthritic joints.
- To ascertain the content levels of substance P and prostaglandin E2 in the synovial fluid, a radioimmunoassay medical technique was applied.
- Using radiographs, the joints were scored numerically to reflect the severity of the lesions present. Also, the anatomic location of each joint was assigned a numeric score.
Research Findings
- The results showed that osteoarthritic joints had significantly increased median concentrations of substance P and prostaglandin E2 compared to normal joints.
- A strong correlation was established between levels of substance P and prostaglandin E2 in the synovial fluid.
- No correlation, however, was found between the synovial fluid’s substance P concentration and the joint’s anatomic location.
- The study also found that the radiographic scores of osteoarthritic joints had no correlation with the concentrations of substance P or prostaglandin E2.
Conclusion
- The study concluded that there’s a correlation between concentrations of substance P and prostaglandin E2 in the synovial fluid obtained from normal and osteoarthritic joints.
- Importantly, these substances’ presence can’t be predicted by observing the joint’s radiographic appearance or its anatomic location.
- The researchers suggest that substance P and prostaglandin E2 could play a significant and interconnected role in the development of osteoarthritis, emphasizing the importance of neurogenic inflammation in horses.
Cite This Article
APA
Kirker-Head CA, Chandna VK, Agarwal RK, Morris EA, Tidwell A, O'Callaghan MW, Rand W, Kumar MS.
(2000).
Concentrations of substance P and prostaglandin E2 in synovial fluid of normal and abnormal joints of horses.
Am J Vet Res, 61(6), 714-718.
https://doi.org/10.2460/ajvr.2000.61.714 Publication
Researcher Affiliations
- Department of Surgery, School of Veterinary Medicine, Tufts University, North Grafton, MA 01536, USA.
MeSH Terms
- Animals
- Dinoprostone / analysis
- Dinoprostone / biosynthesis
- Female
- Horse Diseases / etiology
- Horse Diseases / pathology
- Horses
- Joint Diseases / diagnostic imaging
- Joint Diseases / etiology
- Joint Diseases / veterinary
- Male
- Osteoarthritis / etiology
- Osteoarthritis / pathology
- Osteoarthritis / veterinary
- Radiography
- Radioimmunoassay / veterinary
- Statistics, Nonparametric
- Substance P / analysis
- Substance P / biosynthesis
- Synovial Fluid / chemistry
- Synovial Fluid / metabolism
Citations
This article has been cited 9 times.- Scholler D, Zablotski Y, May A. Evaluation of Substance P as a New Stress Parameter in Horses in a Stress Model Involving Four Different Stress Levels.. Animals (Basel) 2023 Mar 24;13(7).
- Pezzanite LM, Chow L, Griffenhagen GM, Bass L, Goodrich LR, Impastato R, Dow S. Distinct differences in immunological properties of equine orthobiologics revealed by functional and transcriptomic analysis using an activated macrophage readout system.. Front Vet Sci 2023;10:1109473.
- McParland TJ, Horne CR, Robertson JB, Schnabel LV, Nelson NC. Alterations to the synovial invaginations of the navicular bone are associated with pathology of both the navicular apparatus and distal interphalangeal joint when evaluated using high field MRI.. Vet Radiol Ultrasound 2023 Jan;64(1):9-17.
- Seabaugh KA, Barrett MF, Rao S, McIlwraith CW, Frisbie DD. Examining the Effects of the Oral Supplement Biota orientalis in the Osteochondral Fragment-Exercise Model of Osteoarthritis in the Horse.. Front Vet Sci 2022;9:858391.
- Menarim BC, Gillis KH, Oliver A, Ngo Y, Werre SR, Barrett SH, Rodgerson DH, Dahlgren LA. Macrophage Activation in the Synovium of Healthy and Osteoarthritic Equine Joints.. Front Vet Sci 2020;7:568756.
- Niemelä TM, Tulamo RM, Aaltonen K, Sankari SM, Hielm-Björkman AK. Changes in biomarkers in equine synovial fluid two weeks after intra-articular hyaluronan treatment: a randomised double-blind clinical trial.. BMC Vet Res 2018 Jun 15;14(1):186.
- MacNicol JL, Lindinger MI, Pearson W. A time-course evaluation of inflammatory and oxidative markers following high-intensity exercise in horses: a pilot study.. J Appl Physiol (1985) 2018 Apr 1;124(4):860-865.
- Heikkilä HM, Hielm-Björkman AK, Innes JF, Laitinen-Vapaavuori OM. The effect of intra-articular botulinum toxin A on substance P, prostaglandin E(2), and tumor necrosis factor alpha in the canine osteoarthritic joint.. BMC Vet Res 2017 Mar 21;13(1):74.
- Baccarin RY, Rasera L, Machado TS, Michelacci YM. Relevance of synovial fluid chondroitin sulphate as a biomarker to monitor polo pony joints.. Can J Vet Res 2014 Jan;78(1):50-60.
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