Cryopreservation does not affect the stem characteristics of multipotent cells isolated from equine peripheral blood.
Abstract: Mammalian adult stem cells show, in vitro, extensive differentiative ability and may represent a versatile tool for tissue regenerative purposes, even after long-term storage. Multipotent stem cells isolated from horse blood have been shown to possess the capacity to differentiate into diverse mesenchymal lineages although their full characterization is still at an early stage. The aim of this study was to examine the effects of cryopreservation on stemness characteristics of adult equine mesenchymal stem cells isolated from peripheral blood (ePB-MSC). Each sample of ePB-MSC was analyzed immediately and then after being frozen in liquid nitrogen for 10-12 months. After cryopreservation, cells conserved their morphology, alkaline phosphatase positivity, telomerase activity, karyotype profile, proliferation rate, and CD expression pattern. We characterized ePB-MSC as cells expressing CD44, CD90, CD117, and CD13, but not CD34 and CD45. Finally, freezing and storing ePB-MSC did not change their adipogenic, osteogenic, and myogenic differentiative potential, as analyzed by histochemistry, immunofluorescence, and polymerase chain reaction expression analyses. Overall, our results demonstrate that cryopreservation of ePB-MSC provides a convenient tool for in vitro applications, because cryopreserved cells possess the same stem characteristics as freshly isolated cells. Moreover, the feasibility of maintaining stem cell features of ePB-MSC after long-term storage has important implications for autologous cellular-based therapy in veterinary medicine.
Publication Date: 2009-10-21 PubMed ID: 19839741DOI: 10.1089/ten.TEC.2009.0512Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article presents a study on the impact of cryopreservation on the stem characteristics of multipotent cells isolated from equine peripheral blood. It suggests that cryopreservation does not alter the cellular properties, thus indicating its potential usefulness in both in vitro applications and cellular-based therapy.
Objective of the Study
- The primary objective of this study was to analyze the effects of cryopreservation on stemness characteristics of adult equine mesenchymal stem cells isolated from peripheral blood (ePB-MSC).
Methodology
- The study examined each sample of ePB-MSC both immediately after isolation and after being frozen in liquid nitrogen for a period between 10 and 12 months.
- Several parameters, including morphology, alkaline phosphatase positivity, telomerase activity, karyotype profile, proliferation rate, and CD expression pattern, were analyzed to understand the impact of cryopreservation.
- The cells were also characterized by their expression of CD44, CD90, CD117, and CD13, but the absence of CD34 and CD45.
Findings
- The results indicated that the cells retained all characteristics after the cryopreservation process, including their adipogenic, osteogenic, and myogenic differentiative potential as confirmed through histochemistry, immunofluorescence, and polymerase chain reaction expression analyses.
Implications
- The findings have significant implications for veterinary medicine as they suggest that ePB-MSC can be preserved effectively for long periods without compromising their stem features. This opens up possibilities for autologous cellular-based therapy using these cells.
- The study’s results also imply that cryopreserved equine cells could serve as a valuable resource for in vitro applications, due to their preservation of essential stem characteristics.
Cite This Article
APA
Martinello T, Bronzini I, Maccatrozzo L, Iacopetti I, Sampaolesi M, Mascarello F, Patruno M.
(2009).
Cryopreservation does not affect the stem characteristics of multipotent cells isolated from equine peripheral blood.
Tissue Eng Part C Methods, 16(4), 771-781.
https://doi.org/10.1089/ten.TEC.2009.0512 Publication
Researcher Affiliations
- Department of Experimental Veterinary Sciences, University of Padova, Legnaro, Italy.
MeSH Terms
- Animals
- Biomarkers / metabolism
- Blood Cells / cytology
- Cell Adhesion
- Cell Differentiation
- Cell Membrane / metabolism
- Cell Proliferation
- Cell Separation / methods
- Cell Shape
- Cryopreservation / methods
- Gene Expression Profiling
- Horses / blood
- Immunophenotyping
- Kinetics
- Multipotent Stem Cells / cytology
- Multipotent Stem Cells / metabolism
- Telomerase / metabolism
Citations
This article has been cited 21 times.- Quintero Sierra LA, Biswas R, Conti A, Busato A, Ossanna R, Zingaretti N, Parodi PC, Conti G, Riccio M, Sbarbati A, De Francesco F. Highly Pluripotent Adipose-Derived Stem Cell-Enriched Nanofat: A Novel Translational System in Stem Cell Therapy.. Cell Transplant 2023 Jan-Dec;32:9636897231175968.
- Jammes M, Contentin R, Cassé F, Galéra P. Equine osteoarthritis: Strategies to enhance mesenchymal stromal cell-based acellular therapies.. Front Vet Sci 2023;10:1115774.
- Hagen A, Niebert S, Brandt VP, Holland H, Melzer M, Wehrend A, Burk J. Functional properties of equine adipose-derived mesenchymal stromal cells cultured with equine platelet lysate.. Front Vet Sci 2022;9:890302.
- Taylor SD, Serpa PBS, Santos AP, Hart KA, Vaughn SA, Moore GE, Mukhopadhyay A, Page AE. Effects of intravenous administration of peripheral blood-derived mesenchymal stromal cells after infusion of lipopolysaccharide in horses.. J Vet Intern Med 2022 Jul;36(4):1491-1501.
- Melotti L, Carolo A, Elshazly N, Boesso F, Da Dalt L, Gabai G, Perazzi A, Iacopetti I, Patruno M. Case Report: Repeated Intralesional Injections of Autologous Mesenchymal Stem Cells Combined With Platelet-Rich Plasma for Superficial Digital Flexor Tendon Healing in a Show Jumping Horse.. Front Vet Sci 2022;9:843131.
- Di Bella S, Cannella V, Mira F, Di Marco P, Lastra A, Gucciardi F, Purpari G, Guercio A. The Effect of a 7 Year-Long Cryopreservation on Stemness Features of Canine Adipose-Derived Mesenchymal Stem Cells (cAD-MSC).. Animals (Basel) 2021 Jun 11;11(6).
- Mocchi M, Dotti S, Bue MD, Villa R, Bari E, Perteghella S, Torre ML, Grolli S. Veterinary Regenerative Medicine for Musculoskeletal Disorders: Can Mesenchymal Stem/Stromal Cells and Their Secretome Be the New Frontier?. Cells 2020 Jun 11;9(6).
- Gugjoo MB, Hussain S, Amarpal, Shah RA, Dhama K. Mesenchymal Stem Cell-Mediated Immuno-Modulatory and Anti- Inflammatory Mechanisms in Immune and Allergic Disorders.. Recent Pat Inflamm Allergy Drug Discov 2020;14(1):3-14.
- Kusuma GD, Barabadi M, Tan JL, Morton DAV, Frith JE, Lim R. To Protect and to Preserve: Novel Preservation Strategies for Extracellular Vesicles.. Front Pharmacol 2018;9:1199.
- Martinello T, Gomiero C, Perazzi A, Iacopetti I, Gemignani F, DeBenedictis GM, Ferro S, Zuin M, Martines E, Brun P, Maccatrozzo L, Chiers K, Spaas JH, Patruno M. Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin.. BMC Vet Res 2018 Jun 25;14(1):202.
- Duan W, Lopez MJ, Hicok K. Adult multipotent stromal cell cryopreservation: Pluses and pitfalls.. Vet Surg 2018 Jan;47(1):19-29.
- Patruno M, Gomiero C, Sacchetto R, Topel O, Negro A, Martinello T. Tat-MyoD fused proteins, together with C2c12 conditioned medium, are able to induce equine adult mesenchimal stem cells towards the myogenic fate.. Vet Res Commun 2017 Sep;41(3):211-217.
- Gomiero C, Bertolutti G, Martinello T, Van Bruaene N, Broeckx SY, Patruno M, Spaas JH. Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.. Vet Res Commun 2016 Mar;40(1):39-48.
- Lombana KG, Goodrich LR, Phillips JN, Kisiday JD, Ruple-Czerniak A, McIlwraith CW. An Investigation of Equine Mesenchymal Stem Cell Characteristics from Different Harvest Sites: More Similar Than Not.. Front Vet Sci 2015;2:67.
- Maia L, da Cruz Landim-Alvarenga F, Taffarel MO, de Moraes CN, Machado GF, Melo GD, Amorim RM. Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.. BMC Vet Res 2015 Mar 15;11:63.
- Benedetto CD, Barbaglio A, Martinello T, Alongi V, Fassini D, Cullorà E, Patruno M, Bonasoro F, Barbosa MA, Carnevali MD, Sugni M. Production, characterization and biocompatibility of marine collagen matrices from an alternative and sustainable source: the sea urchin Paracentrotus lividus.. Mar Drugs 2014 Sep 24;12(9):4912-33.
- Broeckx S, Zimmerman M, Crocetti S, Suls M, Mariën T, Ferguson SJ, Chiers K, Duchateau L, Franco-Obregón A, Wuertz K, Spaas JH. Regenerative therapies for equine degenerative joint disease: a preliminary study.. PLoS One 2014;9(1):e85917.
- De Schauwer C, Goossens K, Piepers S, Hoogewijs MK, Govaere JL, Smits K, Meyer E, Van Soom A, Van de Walle GR. Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources.. Stem Cell Res Ther 2014 Jan 13;5(1):6.
- Naaldijk Y, Staude M, Fedorova V, Stolzing A. Effect of different freezing rates during cryopreservation of rat mesenchymal stem cells using combinations of hydroxyethyl starch and dimethylsulfoxide.. BMC Biotechnol 2012 Aug 13;12:49.
- Mensing N, Gasse H, Hambruch N, Haeger JD, Pfarrer C, Staszyk C. Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.. BMC Vet Res 2011 Aug 2;7:42.
- Raabe O, Shell K, Würtz A, Reich CM, Wenisch S, Arnhold S. Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells.. Vet Res Commun 2011 Aug;35(6):355-65.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
