Cytogenetic localization of 136 genes in the horse: comparative mapping with the human genome.
Abstract: The aim of this study was to increase the number of type I markers on the horse cytogenetic map and to improve comparison with maps of other species, thus facilitating positional candidate cloning studies. BAC clones from two different sources were FISH mapped: homologous horse BAC clones selected from our newly extended BAC library using consensus primer sequences and heterologous goat BAC clones. We report the localization of 136 genes on the horse cytogenetic map, almost doubling the number of cytogenetically mapped genes with 48 localizations from horse BAC clones and 88 from goat BAC clones. For the first time, genes were mapped to ECA13p, ECA29, and probably ECA30. A total of 284 genes are now FISH mapped on the horse chromosomes. Comparison with the human map defines 113 conserved segments that include new homologous segments not identified by Zoo-FISH on ECA7 and ECA13p.
Publication Date: 2002-10-09 PubMed ID: 12370783DOI: 10.1007/s00335-001-2137-4Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research study sought to enhance type I markers in the horse cytogenetic map, facilitating easier comparative studies with other species. The study significantly increased the number of genes localized on the horse cytogenetic map via BAC clones from horses and goats.
Objective and Methodology of the Study
- The central goal of this study was to amplify the count of type I markers on the horse cytogenetic map. Increasing this marker count aids in a more efficient comparison with the genetic maps of other species, consequently simplifying positional candidate cloning investigations.
- The methodology comprised the usage and mapping of BAC (Bacterial Artificial Chromosomes) clones stemming from two different sources: homologous horse BAC clones and heterologous goat BAC clones.
- BAC clones from these two sources were FISH (Fluorescence In Situ Hybridization) mapped on to the horse’s genetic map.
Findings of the Study
- A substantial increase in localized genes was reported on the horse’s cytogenetic map. The previous number of cytogenetically mapped genes was nearly doubled as a result of the study, with 48 localizations from horse BAC clones and 88 from goat BAC clones.
- The research also marked the first-ever mapping of genes to ECA13p, ECA29, and presumably ECA30 on the horse’s genetic map.
- A total of 284 genes were eventually FISH mapped onto the horse’s chromosomes.
Comparative Analysis and Conclusion
- A comparative analysis with the human genetic map depicted 113 conserved segments. This comparison revealed new homologous segments not earlier identified by Zoo-FISH on ECA7 and ECA13p.
- The findings of this research are instrumental in enhancing comparative cytogenetic studies between the horse and other species, thereby advancing the understanding and potential interventions in genetic disorders.
Cite This Article
APA
Milenkovic D, Oustry-Vaiman A, Lear TL, Billault A, Mariat D, Piumi F, Schibler L, Cribiu E, Guérin G.
(2002).
Cytogenetic localization of 136 genes in the horse: comparative mapping with the human genome.
Mamm Genome, 13(9), 524-534.
https://doi.org/10.1007/s00335-001-2137-4 Publication
Researcher Affiliations
- Institut National de la Recherche Agronomique, Centre de Recherches de Jouy, Laboratoire de Génétique biochimique et de Cytogé, Département de Génétique animale, 78352 Jouy-en-Josas Cedex, France.
MeSH Terms
- Animals
- Base Sequence
- Chromosome Mapping
- Chromosomes, Artificial, Bacterial / genetics
- Cytogenetics
- DNA / genetics
- Genome, Human
- Horses / genetics
- Humans
- In Situ Hybridization, Fluorescence
- Species Specificity
Citations
This article has been cited 15 times.- Janečka JE, Davis BW, Ghosh S, Paria N, Das PJ, Orlando L, Schubert M, Nielsen MK, Stout TAE, Brashear W, Li G, Johnson CD, Metz RP, Zadjali AMA, Love CC, Varner DD, Bellott DW, Murphy WJ, Chowdhary BP, Raudsepp T. Horse Y chromosome assembly displays unique evolutionary features and putative stallion fertility genes.. Nat Commun 2018 Jul 27;9(1):2945.
- Finno CJ, Bannasch DL. Applied equine genetics.. Equine Vet J 2014 Sep;46(5):538-44.
- Dall'Olio S, Davoli R, Costa LN. Analysis of a candidate gene for behavioural trait variability in horses: catechol-O-methyltransferase (COMT).. Vet Res Commun 2009 Sep;33 Suppl 1:277-9.
- Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM, Brinkmeyer-Langford C, Lee EJ, Agarwala R, Stallknecht-Rice E, Schäffer AA, Skow LC, Tozaki T, Yasue H, Penedo MC, Lyons LA, Khazanehdari KA, Binns MM, MacLeod JN, Distl O, Guérin G, Leeb T, Mickelson JR, Chowdhary BP. A 4,103 marker integrated physical and comparative map of the horse genome.. Cytogenet Genome Res 2008;122(1):28-36.
- Trifonov VA, Stanyon R, Nesterenko AI, Fu B, Perelman PL, O'Brien PC, Stone G, Rubtsova NV, Houck ML, Robinson TJ, Ferguson-Smith MA, Dobigny G, Graphodatsky AS, Yang F. Multidirectional cross-species painting illuminates the history of karyotypic evolution in Perissodactyla.. Chromosome Res 2008;16(1):89-107.
- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence.. Chromosome Res 2008;16(1):109-27.
- Dierks C, Löhring K, Lampe V, Wittwer C, Drögemüller C, Distl O. Genome-wide search for markers associated with osteochondrosis in Hanoverian warmblood horses.. Mamm Genome 2007 Oct;18(10):739-47.
- Di Meo GP, Perucatti A, Floriot S, Hayes H, Schibler L, Rullo R, Incarnato D, Ferretti L, Cockett N, Cribiu E, Williams JL, Eggen A, Iannuzzi L. An advanced sheep (Ovis aries, 2n = 54) cytogenetic map and assignment of 88 new autosomal loci by fluorescence in situ hybridization and R-banding.. Anim Genet 2007 Jun;38(3):233-40.
- Antonacci R, Vaccarelli G, Di Meo GP, Piccinni B, Miccoli MC, Cribiu EP, Perucatti A, Iannuzzi L, Ciccarese S. Molecular in situ hybridization analysis of sheep and goat BAC clones identifies the transcriptional orientation of T cell receptor gamma genes on chromosome 4 in bovids.. Vet Res Commun 2007 Nov;31(8):977-83.
- Brinkmeyer-Langford C, Raudsepp T, Lee EJ, Goh G, Schäffer AA, Agarwala R, Wagner ML, Tozaki T, Skow LC, Womack JE, Mickelson JR, Chowdhary BP. A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals.. Mamm Genome 2005 Aug;16(8):631-49.
- Horín P, Smola J, Matiasovic J, Vyskocil M, Lukeszová L, Tomanová K, Králík P, Glasnák V, Schröffelová D, Knoll A, Sedlinská M, Krenková L, Jahn P. Polymorphisms in equine immune response genes and their associations with infections.. Mamm Genome 2004 Oct;15(10):843-50.
- Raudsepp T, Santani A, Wallner B, Kata SR, Ren C, Zhang HB, Womack JE, Skow LC, Chowdhary BP. A detailed physical map of the horse Y chromosome.. Proc Natl Acad Sci U S A 2004 Jun 22;101(25):9321-6.
- Yang F, Fu B, O'Brien PC, Nie W, Ryder OA, Ferguson-Smith MA. Refined genome-wide comparative map of the domestic horse, donkey and human based on cross-species chromosome painting: insight into the occasional fertility of mules.. Chromosome Res 2004;12(1):65-76.
- Raudsepp T, Lee EJ, Kata SR, Brinkmeyer C, Mickelson JR, Skow LC, Womack JE, Chowdhary BP. Exceptional conservation of horse-human gene order on X chromosome revealed by high-resolution radiation hybrid mapping.. Proc Natl Acad Sci U S A 2004 Feb 24;101(8):2386-91.
- Chowdhary BP, Raudsepp T, Kata SR, Goh G, Millon LV, Allan V, Piumi F, Guérin G, Swinburne J, Binns M, Lear TL, Mickelson J, Murray J, Antczak DF, Womack JE, Skow LC. The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes.. Genome Res 2003 Apr;13(4):742-51.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
