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Stem cells and development2012; 22(4); 611-621; doi: 10.1089/scd.2012.0052

Derivation and characterization of induced pluripotent stem cells from equine fibroblasts.

Abstract: Pluripotent stem cells offer unprecedented potential not only for human medicine but also for veterinary medicine, particularly in relation to the horse. Induced pluripotent stem cells (iPSCs) are particularly promising, as they are functionally similar to embryonic stem cells and can be generated in vitro in a patient-specific manner. In this study, we report the generation of equine iPSCs from skin fibroblasts obtained from a foal and reprogrammed using viral vectors coding for murine Oct4, Sox2, c-Myc, and Klf4 sequences. The reprogrammed cell lines were morphologically similar to iPSCs reported from other species and could be stably maintained over more than 30 passages. Immunostaining and polymerase chain reaction analyses revealed that these cell lines expressed an array of endogenous markers associated with pluripotency, including OCT4, SOX2, NANOG, REX1, LIN28, SSEA1, SSEA4, and TRA1-60. Furthermore, under the appropriate conditions, the equine iPSCs readily formed embryoid bodies and differentiated in vitro into cells expressing markers of ectoderm, mesoderm, and endoderm, and when injected into immunodeficient mice, gave raise to tumors containing differentiated derivatives of the 3 germ layers. Finally, we also reprogrammed fibroblasts from a 2-year-old horse. The reprogrammed cells were similar to iPSCs derived from neonatal fibroblasts in terms of morphology, expression of pluripotency markers, and differentiation ability. The generation of these novel cell lines constitutes an important step toward the understanding of pluripotency in the horse, and paves the way for iPSC technology to potentially become a powerful research and clinical tool in veterinary biomedicine.
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Publication Date: 2012-09-28 PubMed ID: 22897112PubMed Central: PMC3564467DOI: 10.1089/scd.2012.0052Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study focuses on the development of pluripotent stem cells from horse fibroblasts, which could be a significant step in veterinary biomedicine. The creation of these equine induced pluripotent stem cells (iPSCs) was achieved through reprogramming methods, using specific sequences to help form reprogrammed cell lines. The study showed that these cell lines were able to differentiate into various cell types, thus demonstrating their pluripotency.

Generation of equine induced Pluripotent Stem Cells

  • The researchers generated equine iPSCs from skin fibroblasts taken from a young horse, using viral vectors coded with specific sequences (Oct4, Sox2, c-Myc, and Klf4) found in murine (mice).
  • The cells were reprogrammed to form cell lines, which maintained their stability for more than 30 passages. This high level of stability is a critical factor for clinical or lab use.

Verification of Pluripotency markers

  • The equine iPSCs demonstrated the expression of various pluripotency markers including OCT4, SOX2, NANOG, REX1, LIN28, SSEA1, SSEA4, and TRA1-60, which were confirmed by immunostaining and polymerase chain reaction (PCR) analyses.
  • The expression of these endogenous markers is a good indicator of the pluripotency of these reprogrammed cell lines.

Differentiation of equine iPSCs

  • The research further assessed the differentiation ability of these equine iPSCs by forming embryoid bodies and differentiating into cells expressing markers of ectoderm, mesoderm, and endoderm under suitable conditions.
  • The cells also formed tumors, comprising differentiated derivatives of the three germ layers when injected into immunodeficient mice, further corroborating the pluripotency of these cells.

Reprogramming of fibroblasts from an older horse

  • In addition to fibroblasts from a young horse, the researchers also reprogrammed fibroblasts from a two-year-old horse.
  • The cells obtained were as pluripotent as those from the young horse in terms of morphology, marker expression, and differentiation ability, which again solidifies the robustness of the reprogramming process implemented in this study.

Implications for Veterinary Biomedicine

  • This study signifies a crucial step towards understanding pluripotency in horses and could represent a powerful research tool in the field of veterinary biomedicine.
  • The creation of these equine iPSCs paves the way for this technology to become potentially valuable for treating various equine diseases or for developing new therapies in the field of veterinary medicine.

Cite This Article

APA
Breton A, Sharma R, Diaz AC, Parham AG, Graham A, Neil C, Whitelaw CB, Milne E, Donadeu FX. (2012). Derivation and characterization of induced pluripotent stem cells from equine fibroblasts. Stem Cells Dev, 22(4), 611-621. https://doi.org/10.1089/scd.2012.0052

Publication

Stem cells and development
ISSN: 1557-8534
NlmUniqueID: 101197107
Country: United States
Language: English
Volume: 22
Issue: 4
Pages: 611-621

Researcher Affiliations

Breton, Amandine
  • The Roslin Institute, University of Edinburgh, Midlothian, United Kingdom.
Sharma, Ruchi
    Diaz, Andrea Catalina
      Parham, Alea Gillian
        Graham, Audrey
          Neil, Claire
            Whitelaw, Christopher Bruce
              Milne, Elspeth
                Donadeu, Francesc Xavier

                  MeSH Terms

                  • Animals
                  • Antigens, Differentiation / biosynthesis
                  • Antigens, Differentiation / genetics
                  • Cell Dedifferentiation
                  • Cells, Cultured
                  • Embryoid Bodies / cytology
                  • Embryoid Bodies / metabolism
                  • Fibroblasts / cytology
                  • Fibroblasts / metabolism
                  • Horses
                  • Humans
                  • Induced Pluripotent Stem Cells / cytology
                  • Induced Pluripotent Stem Cells / metabolism
                  • Kruppel-Like Factor 4
                  • Mice
                  • Transcription Factors / biosynthesis
                  • Transcription Factors / genetics

                  Grant Funding

                  • BBS/E/D/05251442 / Biotechnology and Biological Sciences Research Council
                  • BBS/E/D/05251443 / Biotechnology and Biological Sciences Research Council
                  • BBS/E/D/05251444 / Biotechnology and Biological Sciences Research Council
                  • BBS/E/D/05251445 / Biotechnology and Biological Sciences Research Council

                  References

                  This article includes 43 references
                  1. Tecirlioglu RT, Trounson AO. Embryonic stem cells in companion animals (horses, dogs and cats): present status and future prospects.. Reprod Fertil Dev 2007;19(6):740-7.
                    pubmed: 17714628doi: 10.1071/rd07039google scholar: lookup
                  2. Perkins NR, Reid SW, Morris RS. Risk factors for injury to the superficial digital flexor tendon and suspensory apparatus in Thoroughbred racehorses in New Zealand.. N Z Vet J 2005 Jun;53(3):184-92.
                    pubmed: 16012588doi: 10.1080/00480169.2005.36503google scholar: lookup
                  3. Fortier LA, Potter HG, Rickey EJ, Schnabel LV, Foo LF, Chong LR, Stokol T, Cheetham J, Nixon AJ. Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in the equine model.. J Bone Joint Surg Am 2010 Aug 18;92(10):1927-37.
                    pubmed: 20720135doi: 10.2106/JBJS.I.01284google scholar: lookup
                  4. Ribitsch I, Burk J, Delling U, Geißler C, Gittel C, Jülke H, Brehm W. Basic science and clinical application of stem cells in veterinary medicine.. Adv Biochem Eng Biotechnol 2010;123:219-63.
                    pubmed: 20309674doi: 10.1007/10_2010_66google scholar: lookup
                  5. Vidal MA, Kilroy GE, Johnson JR, Lopez MJ, Moore RM, Gimble JM. Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells: adipogenic and osteogenic capacity.. Vet Surg 2006 Oct;35(7):601-10.
                    pubmed: 17026544doi: 10.1111/j.1532-950X.2006.00197.xgoogle scholar: lookup
                  6. Martins-Taylor K, Xu RH. Determinants of pluripotency: from avian, rodents, to primates.. J Cell Biochem 2010 Jan 1;109(1):16-25.
                    pubmed: 19937733doi: 10.1002/jcb.22402google scholar: lookup
                  7. Talbot NC, Blomberg Le Ann. The pursuit of ES cell lines of domesticated ungulates.. Stem Cell Rev 2008 Sep;4(3):235-54.
                    pubmed: 18612851doi: 10.1007/s12015-008-9026-0google scholar: lookup
                  8. Saito S, Ugai H, Sawai K, Yamamoto Y, Minamihashi A, Kurosaka K, Kobayashi Y, Murata T, Obata Y, Yokoyama K. Isolation of embryonic stem-like cells from equine blastocysts and their differentiation in vitro.. FEBS Lett 2002 Nov 20;531(3):389-96.
                    pubmed: 12435581doi: 10.1016/s0014-5793(02)03550-0google scholar: lookup
                  9. Li X, Zhou SG, Imreh MP, Ahrlund-Richter L, Allen WR. Horse embryonic stem cell lines from the proliferation of inner cell mass cells.. Stem Cells Dev 2006 Aug;15(4):523-31.
                    pubmed: 16978056doi: 10.1089/scd.2006.15.523google scholar: lookup
                  10. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.. Cell 2006 Aug 25;126(4):663-76.
                    pubmed: 16904174doi: 10.1016/j.cell.2006.07.024google scholar: lookup
                  11. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.. Cell 2007 Nov 30;131(5):861-72.
                    pubmed: 18035408doi: 10.1016/j.cell.2007.11.019google scholar: lookup
                  12. Liu H, Zhu F, Yong J, Zhang P, Hou P, Li H, Jiang W, Cai J, Liu M, Cui K, Qu X, Xiang T, Lu D, Chi X, Gao G, Ji W, Ding M, Deng H. Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts.. Cell Stem Cell 2008 Dec 4;3(6):587-90.
                    pubmed: 19041774doi: 10.1016/j.stem.2008.10.014google scholar: lookup
                  13. Liao J, Cui C, Chen S, Ren J, Chen J, Gao Y, Li H, Jia N, Cheng L, Xiao H, Xiao L. Generation of induced pluripotent stem cell lines from adult rat cells.. Cell Stem Cell 2009 Jan 9;4(1):11-5.
                    pubmed: 19097959doi: 10.1016/j.stem.2008.11.013google scholar: lookup
                  14. Ezashi T, Telugu BP, Alexenko AP, Sachdev S, Sinha S, Roberts RM. Derivation of induced pluripotent stem cells from pig somatic cells.. Proc Natl Acad Sci U S A 2009 Jul 7;106(27):10993-8.
                    pmc: PMC2698893pubmed: 19541600doi: 10.1073/pnas.0905284106google scholar: lookup
                  15. Esteban MA, Xu J, Yang J, Peng M, Qin D, Li W, Jiang Z, Chen J, Deng K, Zhong M, Cai J, Lai L, Pei D. Generation of induced pluripotent stem cell lines from Tibetan miniature pig.. J Biol Chem 2009 Jun 26;284(26):17634-40.
                    pmc: PMC2719402pubmed: 19376775doi: 10.1074/jbc.M109.008938google scholar: lookup
                  16. Vaags AK, Rosic-Kablar S, Gartley CJ, Zheng YZ, Chesney A, Villagómez DA, Kruth SA, Hough MR. Derivation and characterization of canine embryonic stem cell lines with in vitro and in vivo differentiation potential.. Stem Cells 2009 Feb;27(2):329-40.
                    pubmed: 19038794doi: 10.1634/stemcells.2008-0433google scholar: lookup
                  17. Luo J, Suhr ST, Chang EA, Wang K, Ross PJ, Nelson LL, Venta PJ, Knott JG, Cibelli JB. Generation of leukemia inhibitory factor and basic fibroblast growth factor-dependent induced pluripotent stem cells from canine adult somatic cells.. Stem Cells Dev 2011 Oct;20(10):1669-78.
                    pmc: PMC3210032pubmed: 21495906doi: 10.1089/scd.2011.0127google scholar: lookup
                  18. Honda A, Hirose M, Hatori M, Matoba S, Miyoshi H, Inoue K, Ogura A. Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine.. J Biol Chem 2010 Oct 8;285(41):31362-9.
                    pmc: PMC2951210pubmed: 20670936doi: 10.1074/jbc.M110.150540google scholar: lookup
                  19. Wu Y, Zhang Y, Mishra A, Tardif SD, Hornsby PJ. Generation of induced pluripotent stem cells from newborn marmoset skin fibroblasts.. Stem Cell Res 2010 May;4(3):180-8.
                    pmc: PMC2875323pubmed: 20363201doi: 10.1016/j.scr.2010.02.003google scholar: lookup
                  20. Bao L, He L, Chen J, Wu Z, Liao J, Rao L, Ren J, Li H, Zhu H, Qian L, Gu Y, Dai H, Xu X, Zhou J, Wang W, Cui C, Xiao L. Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors.. Cell Res 2011 Apr;21(4):600-8.
                    pmc: PMC3203662pubmed: 21221129doi: 10.1038/cr.2011.6google scholar: lookup
                  21. Li Y, Cang M, Lee AS, Zhang K, Liu D. Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.. PLoS One 2011 Jan 6;6(1):e15947.
                    pmc: PMC3017083pubmed: 21253598doi: 10.1371/journal.pone.0015947google scholar: lookup
                  22. Sartori C, DiDomenico AI, Thomson AJ, Milne E, Lillico SG, Burdon TG, Whitelaw CB. Ovine-induced pluripotent stem cells can contribute to chimeric lambs.. Cell Reprogram 2012 Feb;14(1):8-19.
                    pubmed: 22217199doi: 10.1089/cell.2011.0050google scholar: lookup
                  23. Nagy K, Sung HK, Zhang P, Laflamme S, Vincent P, Agha-Mohammadi S, Woltjen K, Monetti C, Michael IP, Smith LC, Nagy A. Induced pluripotent stem cell lines derived from equine fibroblasts.. Stem Cell Rev Rep 2011 Sep;7(3):693-702.
                    pmc: PMC3137777pubmed: 21347602doi: 10.1007/s12015-011-9239-5google scholar: lookup
                  24. Sumer H, Liu J, Malaver-Ortega LF, Lim ML, Khodadadi K, Verma PJ. NANOG is a key factor for induction of pluripotency in bovine adult fibroblasts.. J Anim Sci 2011 Sep;89(9):2708-16.
                    pubmed: 21478453doi: 10.2527/jas.2010-3666google scholar: lookup
                  25. Hochedlinger K, Jaenisch R. Nuclear reprogramming and pluripotency.. Nature 2006 Jun 29;441(7097):1061-7.
                    pubmed: 16810240doi: 10.1038/nature04955google scholar: lookup
                  26. Saha K, Jaenisch R. Technical challenges in using human induced pluripotent stem cells to model disease.. Cell Stem Cell 2009 Dec 4;5(6):584-95.
                    pmc: PMC2921621pubmed: 19951687doi: 10.1016/j.stem.2009.11.009google scholar: lookup
                  27. Pan C, Hicks A, Guan X, Chen H, Bishop CE. SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells.. J Genet Genomics 2010 Apr;37(4):241-8.
                    pmc: PMC3768137pubmed: 20439100doi: 10.1016/S1673-8527(09)60042-4google scholar: lookup
                  28. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics.. Exp Hematol 2003 Nov;31(11):1007-14.
                    pubmed: 14585362
                  29. De Miguel MP, Fuentes-Julián S, Alcaina Y. Pluripotent stem cells: origin, maintenance and induction.. Stem Cell Rev Rep 2010 Dec;6(4):633-49.
                    pubmed: 20669057doi: 10.1007/s12015-010-9170-1google scholar: lookup
                  30. Hackett CH, Greve L, Novakofski KD, Fortier LA. Comparison of gene-specific DNA methylation patterns in equine induced pluripotent stem cell lines with cells derived from equine adult and fetal tissues.. Stem Cells Dev 2012 Jul 1;21(10):1803-11.
                    pmc: PMC3376462pubmed: 21988203doi: 10.1089/scd.2011.0055google scholar: lookup
                  31. Guest DJ, Allen WR. Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts.. Stem Cells Dev 2007 Oct;16(5):789-96.
                    pubmed: 17999600doi: 10.1089/scd.2007.0032google scholar: lookup
                  32. Saito S, Sawai K, Minamihashi A, Ugai H, Murata T, Yokoyama KK. Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells.. Methods Mol Biol 2006;329:59-79.
                    pubmed: 16845984doi: 10.1385/1-59745-037-5:59google scholar: lookup
                  33. Lowry WE, Richter L, Yachechko R, Pyle AD, Tchieu J, Sridharan R, Clark AT, Plath K. Generation of human induced pluripotent stem cells from dermal fibroblasts.. Proc Natl Acad Sci U S A 2008 Feb 26;105(8):2883-8.
                    pmc: PMC2268554pubmed: 18287077doi: 10.1073/pnas.0711983105google scholar: lookup
                  34. Amabile G, Meissner A. Induced pluripotent stem cells: current progress and potential for regenerative medicine.. Trends Mol Med 2009 Feb;15(2):59-68.
                    pubmed: 19162546doi: 10.1016/j.molmed.2008.12.003google scholar: lookup
                  35. Chan EM, Ratanasirintrawoot S, Park IH, Manos PD, Loh YH, Huo H, Miller JD, Hartung O, Rho J, Ince TA, Daley GQ, Schlaeger TM. Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells.. Nat Biotechnol 2009 Nov;27(11):1033-7.
                    pubmed: 19826408doi: 10.1038/nbt.1580google scholar: lookup
                  36. Buehr M, Meek S, Blair K, Yang J, Ure J, Silva J, McLay R, Hall J, Ying QL, Smith A. Capture of authentic embryonic stem cells from rat blastocysts.. Cell 2008 Dec 26;135(7):1287-98.
                    pubmed: 19109897doi: 10.1016/j.cell.2008.12.007google scholar: lookup
                  37. Telugu BP, Ezashi T, Roberts RM. The promise of stem cell research in pigs and other ungulate species.. Stem Cell Rev Rep 2010 Mar;6(1):31-41.
                    pubmed: 19949895doi: 10.1007/s12015-009-9101-1google scholar: lookup
                  38. Telugu BP, Ezashi T, Roberts RM. Porcine induced pluripotent stem cells analogous to naïve and primed embryonic stem cells of the mouse.. Int J Dev Biol 2010;54(11-12):1703-11.
                    pubmed: 21305472doi: 10.1387/ijdb.103200btgoogle scholar: lookup
                  39. Li W, Wei W, Zhu S, Zhu J, Shi Y, Lin T, Hao E, Hayek A, Deng H, Ding S. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors.. Cell Stem Cell 2009 Jan 9;4(1):16-9.
                    pubmed: 19097958doi: 10.1016/j.stem.2008.11.014google scholar: lookup
                  40. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC. Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.. Nat Biotechnol 2008 Nov;26(11):1276-84.
                    pubmed: 18931654doi: 10.1038/nbt.1503google scholar: lookup
                  41. Li W, Ding S. Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches.. Methods Mol Biol 2010;636:293-300.
                    pubmed: 20336530doi: 10.1007/978-1-60761-691-7_18google scholar: lookup
                  42. Wu Z, Chen J, Ren J, Bao L, Liao J, Cui C, Rao L, Li H, Gu Y, Dai H, Zhu H, Teng X, Cheng L, Xiao L. Generation of pig induced pluripotent stem cells with a drug-inducible system.. J Mol Cell Biol 2009 Oct;1(1):46-54.
                    pubmed: 19502222doi: 10.1093/jmcb/mjp003google scholar: lookup
                  43. Barrilleaux B, Knoepfler PS. Inducing iPSCs to escape the dish.. Cell Stem Cell 2011 Aug 5;9(2):103-11.
                    pmc: PMC3500137pubmed: 21816362doi: 10.1016/j.stem.2011.07.006google scholar: lookup

                  Citations

                  This article has been cited 31 times.
                  1. Barrachina L, Arshaghi TE, O'Brien A, Ivanovska A, Barry F. Induced pluripotent stem cells in companion animals: how can we move the field forward?. Front Vet Sci 2023;10:1176772.
                    doi: 10.3389/fvets.2023.1176772pubmed: 37180067google scholar: lookup
                  2. Widerspick L, Steffen JF, Tappe D, Muñoz-Fontela C. Animal Model Alternatives in Filovirus and Bornavirus Research.. Viruses 2023 Jan 4;15(1).
                    doi: 10.3390/v15010158pubmed: 36680198google scholar: lookup
                  3. Knežić T, Janjušević L, Djisalov M, Yodmuang S, Gadjanski I. Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives.. Biomolecules 2022 May 13;12(5).
                    doi: 10.3390/biom12050699pubmed: 35625626google scholar: lookup
                  4. Martínez-Falguera D, Iborra-Egea O, Gálvez-Montón C. iPSC Therapy for Myocardial Infarction in Large Animal Models: Land of Hope and Dreams.. Biomedicines 2021 Dec 5;9(12).
                    doi: 10.3390/biomedicines9121836pubmed: 34944652google scholar: lookup
                  5. Pain B, Baquerre C, Coulpier M. Cerebral organoids and their potential for studies of brain diseases in domestic animals.. Vet Res 2021 May 3;52(1):65.
                    doi: 10.1186/s13567-021-00931-zpubmed: 33941270google scholar: lookup
                  6. Pain B. Organoids in domestic animals: with which stem cells?. Vet Res 2021 Mar 4;52(1):38.
                    doi: 10.1186/s13567-021-00911-3pubmed: 33663614google scholar: lookup
                  7. Kumar D, Talluri TR, Selokar NL, Hyder I, Kues WA. Perspectives of pluripotent stem cells in livestock.. World J Stem Cells 2021 Jan 26;13(1):1-29.
                    doi: 10.4252/wjsc.v13.i1.1pubmed: 33584977google scholar: lookup
                  8. Korody ML, Ford SM, Nguyen TD, Pivaroff CG, Valiente-Alandi I, Peterson SE, Ryder OA, Loring JF. Rewinding Extinction in the Northern White Rhinoceros: Genetically Diverse Induced Pluripotent Stem Cell Bank for Genetic Rescue.. Stem Cells Dev 2021 Feb;30(4):177-189.
                    doi: 10.1089/scd.2021.0001pubmed: 33406994google scholar: lookup
                  9. Scarfone RA, Pena SM, Russell KA, Betts DH, Koch TG. The use of induced pluripotent stem cells in domestic animals: a narrative review.. BMC Vet Res 2020 Dec 8;16(1):477.
                    doi: 10.1186/s12917-020-02696-7pubmed: 33292200google scholar: lookup
                  10. Su Y, Zhu J, Salman S, Tang Y. Induced pluripotent stem cells from farm animals.. J Anim Sci 2020 Nov 1;98(11).
                    doi: 10.1093/jas/skaa343pubmed: 33098420google scholar: lookup
                  11. Endo Y, Kamei KI, Inoue-Murayama M. Genetic Signatures of Evolution of the Pluripotency Gene Regulating Network across Mammals.. Genome Biol Evol 2020 Oct 1;12(10):1806-1818.
                    doi: 10.1093/gbe/evaa169pubmed: 32780791google scholar: lookup
                  12. Bressan FF, Bassanezze V, de Figueiredo Pessôa LV, Sacramento CB, Malta TM, Kashima S, Fantinato Neto P, Strefezzi RF, Pieri NCG, Krieger JE, Covas DT, Meirelles FV. Generation of induced pluripotent stem cells from large domestic animals.. Stem Cell Res Ther 2020 Jun 25;11(1):247.
                    doi: 10.1186/s13287-020-01716-5pubmed: 32586372google scholar: lookup
                  13. Pessôa LVF, Bressan FF, Freude KK. Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.. World J Stem Cells 2019 Aug 26;11(8):491-505.
                    doi: 10.4252/wjsc.v11.i8.491pubmed: 31523369google scholar: lookup
                  14. Shojaee A, Parham A. Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges.. Stem Cell Res Ther 2019 Jun 18;10(1):181.
                    doi: 10.1186/s13287-019-1291-0pubmed: 31215490google scholar: lookup
                  15. Pessôa LVF, Pires PRL, Del Collado M, Pieri NCG, Recchia K, Souza AF, Perecin F, da Silveira JC, de Andrade AFC, Ambrosio CE, Bressan FF, Meirelles FV. Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues.. Stem Cells Int 2019;2019:1393791.
                    doi: 10.1155/2019/1393791pubmed: 31191664google scholar: lookup
                  16. Moro LN, Amin G, Furmento V, Waisman A, Garate X, Neiman G, La Greca A, Santín Velazque NL, Luzzani C, Sevlever GE, Vichera G, Miriuka SG. MicroRNA characterization in equine induced pluripotent stem cells.. PLoS One 2018;13(12):e0207074.
                    doi: 10.1371/journal.pone.0207074pubmed: 30507934google scholar: lookup
                  17. Amilon KR, Cortes-Araya Y, Moore B, Lee S, Lillico S, Breton A, Esteves CL, Donadeu FX. Generation of Functional Myocytes from Equine Induced Pluripotent Stem Cells.. Cell Reprogram 2018 Oct;20(5):275-281.
                    doi: 10.1089/cell.2018.0023pubmed: 30207795google scholar: lookup
                  18. Alkhilaiwi F, Wang L, Zhou D, Raudsepp T, Ghosh S, Paul S, Palechor-Ceron N, Brandt S, Luff J, Liu X, Schlegel R, Yuan H. Long-term expansion of primary equine keratinocytes that maintain the ability to differentiate into stratified epidermis.. Stem Cell Res Ther 2018 Jul 4;9(1):181.
                    doi: 10.1186/s13287-018-0918-xpubmed: 29973296google scholar: lookup
                  19. Baird A, Lindsay T, Everett A, Iyemere V, Paterson YZ, McClellan A, Henson FMD, Guest DJ. Osteoblast differentiation of equine induced pluripotent stem cells.. Biol Open 2018 May 10;7(5).
                    doi: 10.1242/bio.033514pubmed: 29685993google scholar: lookup
                  20. Esteves CL, Sheldrake TA, Mesquita SP, Pesántez JJ, Menghini T, Dawson L, Péault B, Donadeu FX. Isolation and characterization of equine native MSC populations.. Stem Cell Res Ther 2017 Apr 18;8(1):80.
                    doi: 10.1186/s13287-017-0525-2pubmed: 28420427google scholar: lookup
                  21. Olivera R, Moro LN, Jordan R, Luzzani C, Miriuka S, Radrizzani M, Donadeu FX, Vichera G. In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors.. PLoS One 2016;11(10):e0164049.
                    doi: 10.1371/journal.pone.0164049pubmed: 27732616google scholar: lookup
                  22. Ogorevc J, Orehek S, Dovč P. Cellular reprogramming in farm animals: an overview of iPSC generation in the mammalian farm animal species.. J Anim Sci Biotechnol 2016;7:10.
                    doi: 10.1186/s40104-016-0070-3pubmed: 26900466google scholar: lookup
                  23. Soto DA, Ross PJ. Pluripotent stem cells and livestock genetic engineering.. Transgenic Res 2016 Jun;25(3):289-306.
                    doi: 10.1007/s11248-016-9929-5pubmed: 26894405google scholar: lookup
                  24. Grigor'eva EV, Shevchenko AI, Medvedev SP, Mazurok NA, Zhelezova AI, Zakian SM. Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal.. Acta Naturae 2015 Oct-Dec;7(4):56-69.
                    pubmed: 26798492
                  25. Quattrocelli M, Giacomazzi G, Broeckx SY, Ceelen L, Bolca S, Spaas JH, Sampaolesi M. Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates.. Stem Cell Reports 2016 Jan 12;6(1):55-63.
                    doi: 10.1016/j.stemcr.2015.12.005pubmed: 26771353google scholar: lookup
                  26. Donadeu FX, Esteves CL. Prospects and Challenges of Induced Pluripotent Stem Cells in Equine Health.. Front Vet Sci 2015;2:59.
                    doi: 10.3389/fvets.2015.00059pubmed: 26664986google scholar: lookup
                  27. Bavin EP, Smith O, Baird AE, Smith LC, Guest DJ. Equine Induced Pluripotent Stem Cells have a Reduced Tendon Differentiation Capacity Compared to Embryonic Stem Cells.. Front Vet Sci 2015;2:55.
                    doi: 10.3389/fvets.2015.00055pubmed: 26664982google scholar: lookup
                  28. Kumar D, Talluri TR, Anand T, Kues WA. Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals.. World J Stem Cells 2015 Mar 26;7(2):315-28.
                    doi: 10.4252/wjsc.v7.i2.315pubmed: 25815117google scholar: lookup
                  29. Liu G, Rustom N, Litteljohn D, Bobyn J, Rudyk C, Anisman H, Hayley S. Use of induced pluripotent stem cell derived neurons engineered to express BDNF for modulation of stressor related pathology.. Front Cell Neurosci 2014;8:316.
                    doi: 10.3389/fncel.2014.00316pubmed: 25352778google scholar: lookup
                  30. Whitworth DJ, Ovchinnikov DA, Sun J, Fortuna PR, Wolvetang EJ. Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts.. Stem Cells Dev 2014 Jul 1;23(13):1515-23.
                    doi: 10.1089/scd.2013.0461pubmed: 24555755google scholar: lookup
                  31. Iqbal K, Chitwood JL, Meyers-Brown GA, Roser JF, Ross PJ. RNA-seq transcriptome profiling of equine inner cell mass and trophectoderm.. Biol Reprod 2014 Mar;90(3):61.
                    doi: 10.1095/biolreprod.113.113928pubmed: 24478389google scholar: lookup
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