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Home / Equine Research Bank / Animals (Basel) / Deslorelin Slow-Release Implants Delay Ovulation and Increas...
Animals : an open access journal from MDPI2021; 11(6); doi: 10.3390/ani11061600

Deslorelin Slow-Release Implants Delay Ovulation and Increase Plasma AMH Concentration and Small Antral Follicles in Haflinger Mares.

Abstract: There is an increasing interest in the manipulation of ovarian follicular populations in large domestic animals because this could prove beneficial for assisted reproductive techniques such as ovum pick-up (OPU). The aim of the present study was to evaluate the effects of deslorelin slow-release implants (SRI) on the interovulatory interval, antral follicle count (AFC), number of follicles of different size ranges and plasma anti-Muellerian hormone (AMH) concentration in mares. To synchronize their estrous cycles, Haflinger mares (n = 12) were treated twice with a PGF2α analogue. One day after the second injection (day 0), mares received a 9.4 mg deslorelin SRI (group DES, n = 6) or 1.25 mg deslorelin in a short-acting formulation (CON; n = 6), respectively. Regular transrectal ultrasonography of the genital tract was performed and blood samples were collected for the analysis of progesterone, AMH and gonadotrophins. The interval from implant insertion to the first spontaneous ovulation was 23.8 ± 10.5 days in group DES compared to 17.0 ± 3.9 days in group CON (p < 0.05). For the concentrations of LH, FSH and AMH, interactions between time and treatment were detected (p < 0.05). The AFC and the mean number of follicles with 5 to 10, 10 to 15 and 15 to 20 mm in diameter changed over time (p < 0.05). A time x treatment interaction was demonstrated for follicles of 10 to 15 mm in diameter (p < 0.05). The changes in this follicular subpopulation were reflected by increased plasma AMH concentration in group DES. In conclusion, 9.4 mg deslorelin implants show minor effects with regard to estrus suppression in mares, whereas the changes in the subpopulation of small ovarian follicles could be a promising tool for preparation of mares for OPU.
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Publication Date: 2021-05-28 PubMed ID: 34071625PubMed Central: PMC8229780DOI: 10.3390/ani11061600Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the impact of deslorelin slow-release implants on the reproductive hormone cycle of Haflinger mares, with the intention to support assisted reproductive techniques. It found that these implants can influence ovulation cycle, hormone levels, and the number of follicles at different sizes, indicating potential for preparing mares for ovum pick-up procedures.

Objective of the Study

  • The main aim of the study was to assess the effects of deslorelin slow-release implants on various aspects of the reproductive cycle in mares. These aspects included the interovulatory interval (the time between successive ovulations), antral follicle count (number of small follicles in the ovaries), number of follicles of different sizes, and plasma anti-Muellerian hormone (AMH) concentration.

Research Methodology

  • Twelve Haflinger mares were used in the study and were treated with a PGF analogue to synchronize their estrous cycles.
  • Subsequently, these mares received either a 9.4 mg deslorelin slow-release implant (SRI) or a 1.25 mg deslorelin in a short-acting formulation.
  • Regular transrectal ultrasonography was conducted to monitor the genital tract along with collection of blood samples for analysis of progesterone, AMH, and gonadotrophins (hormones that stimulate the ovaries).

Key Findings

  • The study found that the time from implant insertion to the first spontaneous ovulation was longer in the group that received the deslorelin SRI, compared to the other group.
  • Significant interactions between time and treatment was observed for concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and AMH.
  • The size and number of ovarian follicles in the mares changed over time, with a notable interaction between time and treatment for follicles of 10 to 15 mm.
  • The changes in the subpopulation of small ovarian follicles were mirrored by increased plasma AMH concentration in the group that received the deslorelin SRI.

Conclusion

  • While the deslorelin implants showed minor effects in terms of suppressing estrus in mares, their influence on the subpopulation of small ovarian follicles indicated potential for improving outcomes in assisted reproductive techniques such as ovum pick-up.

Cite This Article

APA
Kaps M, Okada CTC, Gautier CM, Aurich J, Aurich C. (2021). Deslorelin Slow-Release Implants Delay Ovulation and Increase Plasma AMH Concentration and Small Antral Follicles in Haflinger Mares. Animals (Basel), 11(6). https://doi.org/10.3390/ani11061600

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 6

Researcher Affiliations

Kaps, Martim
  • Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
Okada, Carolina T C
  • Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
Gautier, Camille M
  • Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
Aurich, Jörg
  • Obstetrics, Gynaecology and Andrology, Department for Small Animals and Horses, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
Aurich, Christine
  • Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Veterinärplatz 1, 1210 Vienna, Austria.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 39 references
  1. Junaidi A, Williamson P.E, Cummins J.M, Martin G.B, Blackberry M.A, Trigg T.E. Use of a new drug delivery formulation of the gonadotrophin-releasing hormone analogue Deslorelin for reversible long-term contraception in male dogs.. Reprod. Fertil. Dev. 2003;15:317–322.
    doi: 10.1071/RD03039pubmed: 14975229google scholar: lookup
  2. Romagnoli S, Stelletta C, Milani C, Gelli D, Falomo M.E, Mollo A. Clinical use of deslorelin for the control of reproduction in the bitch.. Reprod. Domest. Anim. 2009;44:36–39.
    doi: 10.1111/j.1439-0531.2009.01441.xpubmed: 19754533google scholar: lookup
  3. Munson L, Bauman J.E, Asa C.S, Jöchle W, Trigg T.E. Efficacy of the GnRH analogue deslorelin for suppression of oestrous cycles in cats.. J. Reprod. Fertil. Suppl. 2001;57:269–273.
    pubmed: 11787161
  4. Novotny R, Cizek P, Vitasek R, Bartoskova A, Prinosilova P, Janosovska M. Reversible suppression of sexual activity in tomcats with deslorelin implant.. Theriogenology 2012;78:848–857.
    doi: 10.1016/j.theriogenology.2012.03.035pubmed: 22578617google scholar: lookup
  5. D’Occhio M.J, Aspden W.J, Whyte T.R. Controlled, reversible suppression of estrous cycles in beef heifers and cows using agonists of gonadotropin-releasing hormone.. J. Anim. Sci. 1996;74:218–225.
    doi: 10.2527/1996.741218xpubmed: 8778103google scholar: lookup
  6. Kauffold J, Rohrmann H, Boehm J, Wehrend A. Effects of long-term treatment with the GnRH agonist deslorelin (Suprelorin) on sexual function in boars.. Theriogenology 2010;74:733–740.
    doi: 10.1016/j.theriogenology.2010.03.026pubmed: 20570338google scholar: lookup
  7. Goericke-Pesch S. Long-term effects of GnRH agonists on fertility and behaviour.. Reprod. Domest. Anim. 2017;52(Suppl. 2):336–347.
    doi: 10.1111/rda.12898pubmed: 28025851google scholar: lookup
  8. Montovan S.M, Daels P.P, Rivier J, Hughes J.P, Stabenfeldt G.H, Lasley B.L. The effect of a potent GnRH agonist on gonadal and sexual activity in the horse.. Theriogenology 1990;33:1305–1321.
    doi: 10.1016/0093-691X(90)90049-Ygoogle scholar: lookup
  9. Kaps M, Okada C.T.C, Gautier C, Aurich J, Scarlet D, Kuhl J, Aurich C. Transient suppression of ovulatory ovarian function in pony mares after treatment with slow-release deslorelin implants.. Domest. Anim. Endocrinol. 2021;74:106505.
    doi: 10.1016/j.domaniend.2020.106505pubmed: 32846375google scholar: lookup
  10. Farquhar V.J, McCue P.M, Nett T.M, Squires E.L. Effect of deslorelin acetate on gonadotropin secretion and ovarian follicle development in cycling mares.. J. Am. Vet. Med. Assoc. 2001;218:749–752.
    doi: 10.2460/javma.2001.218.749pubmed: 11280410google scholar: lookup
  11. Johnson C.A, Thompson D.L, Cartmill J.A. Pituitary responsiveness to GnRH in mares following deslorelin acetate implantation to hasten ovulation1.. J. Anim. Sci. 2002;80:2681–2687.
    doi: 10.1093/ansci/80.10.2681pubmed: 12413091google scholar: lookup
  12. McCue P.M, Farquhar V.J, Carnevale E.M, Squires E.L. Removal of deslorelin (Ovuplant™) implant 48 h after administration results in normal interovulatory intervals in mares.. Theriogenology 2002;58:865–870.
    doi: 10.1016/S0093-691X(02)00923-8pubmed: 12212887google scholar: lookup
  13. Farquhar V.J, McCue P.M, Carnevale E.M, Nett T.M, Squires E.L. Deslorelin acetate (Ovuplant) therapy in cycling mares: Effect of implant removal on FSH secretion and ovarian function.. Equine Vet. J. 2002;34:417–420.
    doi: 10.2746/042516402776249227pubmed: 12117117google scholar: lookup
  14. Hinrichs K. Assisted reproductive techniques in mares.. Reprod. Domest. Anim. 2018;53:4–13.
    doi: 10.1111/rda.13259pubmed: 30238661google scholar: lookup
  15. Alm H, Torner H, Kanitz W, Becker F, Hinrichs K. Comparison of different methods for the recovery of horse oocytes.. Equine Vet. J. Suppl. 1997:47–50.
    doi: 10.1111/j.2042-3306.1997.tb05099.xpubmed: 9593527google scholar: lookup
  16. Jacobson C.C, Choi Y.-H, Hayden S.S, Hinrichs K. Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injection.. Theriogenology 2010;73:1116–1126.
    doi: 10.1016/j.theriogenology.2010.01.013pubmed: 20202674google scholar: lookup
  17. Galli C, Duchi R, Colleoni S, Lagutina I, Lazzari G. Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses: From the research laboratory to clinical practice.. Theriogenology 2014;81:138–151.
    doi: 10.1016/j.theriogenology.2013.09.008pubmed: 24274418google scholar: lookup
  18. Claes A, Ball B.A, Scoggin K.E, Esteller-Vico A, Kalmar J.J, Conley A.J, Squires E.L, Troedsson M.H.T. The interrelationship between anti-Müllerian hormone, ovarian follicular populations and age in mares.. Equine Vet. J. 2015;47:537–541.
    doi: 10.1111/evj.12328pubmed: 25124401google scholar: lookup
  19. Henneke D.R, Potter G.D, Kreider J.L, Yeates B.F. Relationship between condition score, physical measurements and body fat percentage in mares.. Equine Vet. J. 1983;15:371–372.
    doi: 10.1111/j.2042-3306.1983.tb01826.xpubmed: 6641685google scholar: lookup
  20. Kuhl J, Nagel C, Ille N, Aurich J.E, Aurich C. The PGF2α agonists luprostiol and d-cloprostenol reliably induce luteolysis in luteal phase mares without evoking clinical side effects or a stress response.. Anim. Reprod. Sci. 2016;168:92–99.
    doi: 10.1016/j.anireprosci.2016.02.031pubmed: 26963045google scholar: lookup
  21. Mateu-Sánchez S, Newcombe J.R, Garcés-Narro C, Cuervo-Arango J. The period of the follicular phase during which the uterus of mares shows estrus-like echotexture influences the subsequent pregnancy rate.. Theriogenology 2016;86:1506–1515.
    doi: 10.1016/j.theriogenology.2016.05.009pubmed: 27298152google scholar: lookup
  22. Scarlet D, Wulf M, Kuhl J, Köhne M, Ille N, Conley A.J, Aurich C. Anti-Müllerian hormone profiling in prepubertal horses and its relationship with gonadal function.. Theriogenology 2018;117:72–77.
    doi: 10.1016/j.theriogenology.2018.05.012pubmed: 29784463google scholar: lookup
  23. Jennings M.W, Boime I, Daphna-Iken D, Jablonka-Shariff A, Conley A.J, Colgin M, Bidstrup L.A, Meyers-Brown G.A, Famula T.R, Roser J.F. The efficacy of recombinant equine follicle stimulating hormone (reFSH) to promote follicular growth in mares using a follicular suppression model.. Anim. Reprod. Sci. 2009;116:291–307.
    doi: 10.1016/j.anireprosci.2009.01.013pubmed: 19237253google scholar: lookup
  24. Matteri R.L, Roser J.F, Baldwin D.M, Lipovetsky V, Papkoff H. Characterization of a monoclonal antibody which detects luteinizing hormone from diverse mammalian species.. Domest. Anim. Endocrinol. 1987;4:157–165.
    doi: 10.1016/0739-7240(87)90011-7pubmed: 3507889google scholar: lookup
  25. Roser J.F, Hughes J.P. Prolonged pulsatile administration of gonadotrophin-releasing hormone (GnRH) to fertile stallions.. J. Reprod. Fertil. Suppl. 1991;44:155–168.
    pubmed: 1665513
  26. Junaidi A, Williamson P.E, Martin G.B, Blackberry M.A, Cummins J.M, Trigg T.E. Dose-response studies for pituitary and testicular function in male dogs treated with the GnRH superagonist, deslorelin.. Reprod. Domest. Anim. 2009;44:725–734.
    doi: 10.1111/j.1439-0531.2008.01060.xpubmed: 19769638google scholar: lookup
  27. Gautier C, Schmidt K, Aurich J, Aurich C. Effect of implants containing the GnRH agonist deslorelin on testosterone release and semen characteristics in Shetland stallions.. Anim. Reprod. Sci. 2018;195:230–241.
    doi: 10.1016/j.anireprosci.2018.05.027pubmed: 29859702google scholar: lookup
  28. Checura C.M, Beg M.A, Gastal E.L, Gastal M.O, Wiltbank M.C, Parrish J.J, Ginther O.J. Effect of suppression of FSH with a GnRH antagonist (acyline) before and during follicle deviation in the mare.. Reprod. Domest. Anim. 2009;44:504–511.
    doi: 10.1111/j.1439-0531.2008.01222.xpubmed: 18954386google scholar: lookup
  29. Donadeu F.X, Pedersen H.G. Follicle development in mares.. Reprod. Domest. Anim. 2008;43:224–231.
    doi: 10.1111/j.1439-0531.2008.01166.xpubmed: 18638128google scholar: lookup
  30. Mc Cue P.M, Magee C, Gee E.K. Comparison of compounded deslorlin and hCG for induction of ovulation in mares.. J. Equine Vet. Sci. 2007;27:58–61.
    doi: 10.1016/j.jevs.2006.12.003google scholar: lookup
  31. Ginther O.J, Gastal E.L, Gastal M.O, Beg M.A. Effect of prostaglandin F2α on ovarian, adrenal, and pituitary hormones and on luteal blood flow in mares.. Domest. Anim. Endocrinol. 2007;32:315–328.
    doi: 10.1016/j.domaniend.2006.04.006pubmed: 16698221google scholar: lookup
  32. Utt M.D, Acosta T.J, Wiltbank M.C, Ginther O.J. Acute effects of prostaglandin F(2α) on systemic oxytocin and progesterone concentrations during the mid- or late-luteal phase in mares.. Anim. Reprod. Sci. 2007;97:63–73.
    doi: 10.1016/j.anireprosci.2006.01.009pubmed: 16500050google scholar: lookup
  33. Ginther O.J, Beg M.A. Dynamics of circulating progesterone concentrations before and during Luteolysis: A comparison between cattle and horses.. Biol. Reprod. 2012;86:170.
    doi: 10.1095/biolreprod.112.099820pubmed: 22460665google scholar: lookup
  34. Ball B.A, Conley A.J, MacLaughlin D.T, Grundy S.A, Sabeur K, Liu I.K.M. Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries.. Theriogenology 2008;70:968–977.
    doi: 10.1016/j.theriogenology.2008.05.059pubmed: 18599114google scholar: lookup
  35. Batista E.O.S, Vieira L.M, Sá Filho M.F, Dias E.A.R, Bayeux B.M, Accorsi M.F, Monteiro F.M, Souza A.H, Baruselli P.S, D’Occhio M.J. Ovarian follicular growth suppression by long-term treatment with a GnRH agonist and impact on small follicle number, oocyte yield, and in vitro embryo production in Zebu beef cows.. Theriogenology 2016;85:1680–1687.
    doi: 10.1016/j.theriogenology.2016.01.023pubmed: 26924682google scholar: lookup
  36. Cuervo-Arango J, Claes A.N, Stout T.A.E. Mare and stallion effects on blastocyst production in a commercial equine ovum pick-up-intracytoplasmic sperm injection program.. Reprod. Fertil. Dev. 2019;31:1894–1903.
    doi: 10.1071/RD19201pubmed: 31634435google scholar: lookup
  37. Shabpareh V, Squires E.L, Seidel G.E, Jasko D.J. Methods for collecting and maturing equine oocytes in vitro.. Theriogenology 1993;40:1161–1175.
    doi: 10.1016/0093-691X(93)90287-Fgoogle scholar: lookup
  38. Roser J.F, Meyers-Brown G. Enhancing fertility in mares: Recombinant equine gonadotropins.. J. Equine Vet. Sci. 2019;76:6–13.
    doi: 10.1016/j.jevs.2019.03.004pubmed: 31084750google scholar: lookup
  39. Abreu F.M, Da Coutinho Silva M.A, Cruppe L.H, Mussard M.L, Bridges G.A, Harstine B.R, Smith G.W, Geary T.W, Day M.L. Role of progesterone concentrations during early follicular development in beef cattle: I. Characteristics of LH secretion and oocyte quality.. Anim. Reprod. Sci. 2018;196:59–68.
    doi: 10.1016/j.anireprosci.2018.06.020pubmed: 30149874google scholar: lookup
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