Detection of a bla(SHV) extended-spectrum {beta}-lactamase in Salmonella enterica serovar Newport MDR-AmpC.
Abstract: Salmonella enterica serovar Newport MDR-AmpC expressing TEM-1b and extended-spectrum beta-lactamase SHV-12 was isolated from affected animals during an outbreak of salmonellosis that led to a 3-month closure of one of the largest equine hospitals in the United States.
Publication Date: 2005-11-08 PubMed ID: 16272522PubMed Central: PMC1287834DOI: 10.1128/JCM.43.11.5792-5793.2005Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research identifies a resistance gene in a Salmonella strain that was responsible for an outbreak at a large equine hospital in the United States. This gene allowed the bacteria to withstand treatment using beta-lactam antibiotics.
Research Background
- This research was conducted after an outbreak of salmonellosis led to a three-month closure of one of the largest equine hospitals in the United States.
- Researchers isolated a strain of Salmonella enterica (serovar Newport MDR-AmpC) from the affected animals.
- Strains of this bacteria are common causes of foodborne diseases and deaths worldwide
Key Findings
- The isolated strain contained two notable genes: TEM-1b and SHV-12.
- TEM-1b is a commonly identified gene in beta-lactam antibiotics-resistant bacteria.
- The researchers also detected the SHV-12 gene, an extended-spectrum beta-lactamase (ESBL).
- ESBLs are enzymes that confer resistance to most beta-lactam antibiotics, including penicillins, cephalosporins, and the monobactam aztreonam.
- The presence of SHV-12 is of concern due to its ability to hydrolyze a broad spectrum of beta-lactams, rendering the antibiotic treatment ineffective.
- This is the first report of a bla(SHV) ESBL in a Salmonella enterica serovar Newport strain.
Implications of the Research
- The findings suggest that the management of antibiotic resistance in Salmonella, and particularly in this strain, is a significant challenge.
- It underscores the need for monitoring the spread of resistant strains and the need for the development of new antibiotics.
- By identifying the presence of the SHV-12 gene in this strain, the study contributes to the understanding of the evolution of antibiotic resistance in Salmonella enterica.
- The knowledge can inform strategies to control the spread of antibiotic-resistant bacteria both in veterinary and human medicine.
Cite This Article
APA
Rankin SC, Whichard JM, Joyce K, Stephens L, O'shea K, Aceto H, Munro DS, Benson CE.
(2005).
Detection of a bla(SHV) extended-spectrum {beta}-lactamase in Salmonella enterica serovar Newport MDR-AmpC.
J Clin Microbiol, 43(11), 5792-5793.
https://doi.org/10.1128/JCM.43.11.5792-5793.2005 Publication
Researcher Affiliations
- Salmonella Reference Center, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348-1692, USA. srankin@vet.upenn.edu
MeSH Terms
- Animals
- Bacterial Proteins / genetics
- Bacterial Proteins / metabolism
- DNA Primers
- Disease Outbreaks
- Gene Expression
- Genes, Bacterial / genetics
- Horses
- Hospitals, Animal
- Polymerase Chain Reaction
- Salmonella Infections, Animal / epidemiology
- Salmonella Infections, Animal / microbiology
- Salmonella enterica / enzymology
- Salmonella enterica / genetics
- Salmonella enterica / isolation & purification
- United States / epidemiology
- beta-Lactamases / genetics
- beta-Lactamases / metabolism
References
This article includes 24 references
- Bradford PA, Urban C, Mariano N, Projan SJ, Rahal JJ, Bush K. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC beta-lactamase, and the foss of an outer membrane protein.. Antimicrob Agents Chemother 1997 Mar;41(3):563-9.
- Bush K. New beta-lactamases in gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy.. Clin Infect Dis 2001 Apr 1;32(7):1085-9.
- Carattoli A, Tosini F, Giles WP, Rupp ME, Hinrichs SH, Angulo FJ, Barrett TJ, Fey PD. Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella strains isolated in the United States between 1996 and 1998.. Antimicrob Agents Chemother 2002 May;46(5):1269-72.
- Dunne EF, Fey PD, Kludt P, Reporter R, Mostashari F, Shillam P, Wicklund J, Miller C, Holland B, Stamey K, Barrett TJ, Rasheed JK, Tenover FC, Ribot EM, Angulo FJ. Emergence of domestically acquired ceftriaxone-resistant Salmonella infections associated with AmpC beta-lactamase.. JAMA 2000 Dec 27;284(24):3151-6.
- Fey PD, Safranek TJ, Rupp ME, Dunne EF, Ribot E, Iwen PC, Bradford PA, Angulo FJ, Hinrichs SH. Ceftriaxone-resistant salmonella infection acquired by a child from cattle.. N Engl J Med 2000 Apr 27;342(17):1242-9.
- Giles WP, Benson AK, Olson ME, Hutkins RW, Whichard JM, Winokur PL, Fey PD. DNA sequence analysis of regions surrounding blaCMY-2 from multiple Salmonella plasmid backbones.. Antimicrob Agents Chemother 2004 Aug;48(8):2845-52.
- Gupta A, Fontana J, Crowe C, Bolstorff B, Stout A, Van Duyne S, Hoekstra MP, Whichard JM, Barrett TJ, Angulo FJ. Emergence of multidrug-resistant Salmonella enterica serotype Newport infections resistant to expanded-spectrum cephalosporins in the United States.. J Infect Dis 2003 Dec 1;188(11):1707-16.
- Hanson ND, Moland ES, Hossain A, Neville SA, Gosbell IB, Thomson KS. Unusual Salmonella enterica serotype Typhimurium isolate producing CMY-7, SHV-9 and OXA-30 beta-lactamases.. J Antimicrob Chemother 2002 Jun;49(6):1011-4.
- Hasman H, Mevius D, Veldman K, Olesen I, Aarestrup FM. beta-Lactamases among extended-spectrum beta-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands.. J Antimicrob Chemother 2005 Jul;56(1):115-21.
- Liebana E, Batchelor M, Torres C, Briñas L, Lagos LA, Abdalhamid B, Hanson ND, Martinez-Urtaza J. Pediatric infection due to multiresistant Salmonella enterica serotype Infantis in Honduras.. J Clin Microbiol 2004 Oct;42(10):4885-8.
- Livermore DM, Brown DF. Detection of beta-lactamase-mediated resistance.. J Antimicrob Chemother 2001 Jul;48 Suppl 1:59-64.
- Miriagou V, Tzouvelekis LS, Rossiter S, Tzelepi E, Angulo FJ, Whichard JM. Imipenem resistance in a Salmonella clinical strain due to plasmid-mediated class A carbapenemase KPC-2.. Antimicrob Agents Chemother 2003 Apr;47(4):1297-300.
- Munday CJ, Whitehead GM, Todd NJ, Campbell M, Hawkey PM. Predominance and genetic diversity of community- and hospital-acquired CTX-M extended-spectrum beta-lactamases in York, UK.. J Antimicrob Chemother 2004 Sep;54(3):628-33.
- National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk susceptibility tests, 8th ed. Approved standard.. 2003.
- National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing, 13th informational supplement.. 2003.
- National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, 2nd ed. Approved standard.. 2002.
- Nüesch-Inderbinen MT, Kayser FH, Hächler H. Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12.. Antimicrob Agents Chemother 1997 May;41(5):943-9.
- Rankin SC, Aceto H, Cassidy J, Holt J, Young S, Love B, Tewari D, Munro DS, Benson CE. Molecular characterization of cephalosporin-resistant Salmonella enterica serotype Newport isolates from animals in Pennsylvania.. J Clin Microbiol 2002 Dec;40(12):4679-84.
- Rasheed JK, Jay C, Metchock B, Berkowitz F, Weigel L, Crellin J, Steward C, Hill B, Medeiros AA, Tenover FC. Evolution of extended-spectrum beta-lactam resistance (SHV-8) in a strain of Escherichia coli during multiple episodes of bacteremia.. Antimicrob Agents Chemother 1997 Mar;41(3):647-53.
- Villa L, Mammina C, Miriagou V, Tzouvelekis LS, Tassios PT, Nastasi A, Carattoli A. Multidrug and broad-spectrum cephalosporin resistance among Salmonella enterica serotype enteritidis clinical isolates in southern Italy.. J Clin Microbiol 2002 Jul;40(7):2662-5.
- Weill FX, Demartin M, Tandé D, Espié E, Rakotoarivony I, Grimont PA. SHV-12-like extended-spectrum-beta-lactamase-producing strains of Salmonella enterica serotypes Babelsberg and Enteritidis isolated in France among infants adopted from Mali.. J Clin Microbiol 2004 Jun;42(6):2432-7.
- Winokur PL, Brueggemann A, DeSalvo DL, Hoffmann L, Apley MD, Uhlenhopp EK, Pfaller MA, Doern GV. Animal and human multidrug-resistant, cephalosporin-resistant salmonella isolates expressing a plasmid-mediated CMY-2 AmpC beta-lactamase.. Antimicrob Agents Chemother 2000 Oct;44(10):2777-83.
- Zansky S, Wallace B, Schoonmaker D, Smith P, Ramsay F, Painter J, Gupta A, Kalleri P, Noviello S. Outbreak of multidrug-resistant Salmonella Newport—United States, January-April 2002.. CDC Surveillance Summaries. Morb. Mortal. Wkly. Rep. 2002;51:545-548.
- Zhao S, Qaiyumi S, Friedman S, Singh R, Foley SL, White DG, McDermott PF, Donkar T, Bolin C, Munro S, Baron EJ, Walker RD. Characterization of Salmonella enterica serotype newport isolated from humans and food animals.. J Clin Microbiol 2003 Dec;41(12):5366-71.
Citations
This article has been cited 9 times.- Dor Z, Shnaiderman-Torban A, Kondratyeva K, Davidovich-Cohen M, Rokney A, Steinman A, Navon-Venezia S. Emergence and Spread of Different ESBL-Producing Salmonella enterica Serovars in Hospitalized Horses Sharing a Highly Transferable IncM2 CTX-M-3-Encoding Plasmid. Front Microbiol 2020;11:616032.
- Elbediwi M, Pan H, Zhou X, Rankin SC, Schifferli DM, Yue M. Detection of mcr-9-harbouring ESBL-producing Salmonella Newport isolated from an outbreak in a large-animal teaching hospital in the USA. J Antimicrob Chemother 2021 Mar 12;76(4):1107-1109.
- Pan H, Paudyal N, Li X, Fang W, Yue M. Multiple Food-Animal-Borne Route in Transmission of Antibiotic-Resistant Salmonella Newport to Humans. Front Microbiol 2018;9:23.
- O'Keefe A, Hutton TA, Schifferli DM, Rankin SC. First detection of CTX-M and SHV extended-spectrum beta-lactamases in Escherichia coli urinary tract isolates from dogs and cats in the United States. Antimicrob Agents Chemother 2010 Aug;54(8):3489-92.
- Zhao S, Blickenstaff K, Glenn A, Ayers SL, Friedman SL, Abbott JW, McDermott PF. beta-Lactam resistance in salmonella strains isolated from retail meats in the United States by the National Antimicrobial Resistance Monitoring System between 2002 and 2006. Appl Environ Microbiol 2009 Dec;75(24):7624-30.
- Lindsey RL, Fedorka-Cray PJ, Frye JG, Meinersmann RJ. Inc A/C plasmids are prevalent in multidrug-resistant Salmonella enterica isolates. Appl Environ Microbiol 2009 Apr;75(7):1908-15.
- Tappe D, Müller A, Langen HJ, Frosch M, Stich A. Isolation of Salmonella enterica serotype newport from a partly ruptured splenic abscess in a traveler returning from Zanzibar. J Clin Microbiol 2007 Sep;45(9):3115-7.
- You Y, Rankin SC, Aceto HW, Benson CE, Toth JD, Dou Z. Survival of Salmonella enterica serovar Newport in manure and manure-amended soils. Appl Environ Microbiol 2006 Sep;72(9):5777-83.
- Sadikalay S, Cavé L, Ducat C, Mauriello G, Berchel M, Boismoreau D, Guyomard S, Nazaret S, Talarmin A, Ferdinand S. Tracking Enterobacteria, microbiomes, and antibiotic resistance genes from waste to soil with repeated compost applications. PLoS One 2025;20(8):e0329200.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
