Detection of fibrin deposits in horse tissues by immunohistochemistry.
Abstract: Histochemical and immunohistochemical techniques have been used to detect fibrin deposits in different tissues in humans and experimental animal models with disseminated intravascular coagulation (DIC). Fibrin deposits also have been observed in horses with severe ischemic and inflammatory disorders by histochemical stainings (phosphotungstic acid hematoxylin [PTAH]). Objective: Immunohistochemical (IHC) methods can be used to accurately detect fibrin deposits in horses at risk of DIC. Methods: Tissue-organ samples collected on postmortem examination from 87 horses with severe inflammatory and ischemic gastrointestinal disorders. In addition, tissue samples from 13 horses with colic and colonic obstructions or displacements and from 13 slaughter horses were used as controls. Methods: Tissue samples (kidney, lung, and liver) were stained with PTAH and IHC for blinded histologic examination and comparison. A fibrin score (grades 0 to 4) was established for each tissue sample and for each horse for both techniques. Results: When the IHC method was used, fibrin deposition was observed in 47.1% of the horses with colic with a poor prognosis, compared with 41.4% with PTAH. An agreement of 70% was achieved when both methods were compared, and the lung was confirmed as the most affected organ. Almost none of the colic and slaughter control horses had fibrin deposits in their tissues. Conclusions: IHC technique for fibrin antigens was very effective in the detection and identification of fibrin deposits in equine tissues and may be a reliable technique for the postmortem diagnosis of DIC.
Publication Date: 2007-10-18 PubMed ID: 17939568DOI: 10.1892/0891-6640(2007)21[1083:dofdih]2.0.co;2Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates how immuno-histochemical (IHC) techniques can accurately identify fibrin deposits in horses suffering from severe ischemic and inflammatory illnesses. Fibrin deposits are indicators of a potentially life-threatening condition known as disseminated intravascular coagulation (DIC). The study demonstrated that IHC was a more reliable method for detecting fibrin deposits compared to traditional histochemical staining methods.
Research Methods
The research methods employed in this study include:
- Collecting tissue-organ samples from postmortem examination of 87 horses with severe inflammatory and ischemic gastrointestinal disorders.
- Samples were also obtained from 13 horses with colic and colonic obstructions or displacements, and 13 slaughter horses were used as control groups.
- The tissue samples, which included kidney, lung, and liver tissues, were stained with phosphotungstic acid hematoxylin (PTAH) and IHC. These stained samples were then subjected to blind histological examination and comparative analysis.
- A fibrin score was established for each tissue sample, as well as for each horse, using both PTAH and IHC staining techniques.
Research Findings
The research findings include:
- The IHC method was able to detect fibrin deposition in 47.1% of the horses with colic which is a sign of poor prognosis, as opposed to the 41.4% detection rate for the conventional PTAH staining method.
- When both methods were compared, a correlation of 70% was achieved reinforcing the reliability of the IHC technique.
- The lung was identified as the most affected organ with a higher frequency of detection of fibrin deposits.
- Almost none of the horses from the control groups (those with colic and the slaughter horses) showed fibrin deposits in their tissues. This suggests that the presence of fibrin deposits was more probable in horses with severe inflammatory and ischemic disorders.
Conclusions
The conclusions drawn from the research include:
- The IHC technique for detecting fibrin antigens proved highly effective for identifying fibrin deposits in horse tissues, indicating it may serve as a reliable technique for diagnosing DIC postmortem in horses.
Cite This Article
APA
Cotovio M, Monreal L, Navarro M, Segura D, Prada J, Alves A.
(2007).
Detection of fibrin deposits in horse tissues by immunohistochemistry.
J Vet Intern Med, 21(5), 1083-1089.
https://doi.org/10.1892/0891-6640(2007)21[1083:dofdih]2.0.co;2 Publication
Researcher Affiliations
- Departamento de Ciências Veterinárias, Universidade de Trás-os- Montes e Alto Douro, Vila Real, Portugal.
MeSH Terms
- Animals
- Coloring Agents / chemistry
- Disseminated Intravascular Coagulation / diagnosis
- Disseminated Intravascular Coagulation / metabolism
- Disseminated Intravascular Coagulation / veterinary
- Fibrin / metabolism
- Hematoxylin / chemistry
- Horse Diseases / diagnosis
- Horse Diseases / metabolism
- Horses
- Immunohistochemistry / veterinary
- Kidney / metabolism
- Liver / metabolism
- Lung / metabolism
- Phosphotungstic Acid / chemistry
- Statistics, Nonparametric
Citations
This article has been cited 2 times.- Aceino A, Jeffery U, Piccione J, Hodo CL. Systemic mastocytosis with subcutaneous hemorrhage and edema in a Greyhound dog: case report and review of diagnostic criteria. J Vet Diagn Invest 2021 Jan;33(1):95-100.
- Cesarini C, Cotovio M, Ríos J, Armengou L, Jose-Cunilleras E. Association Between Necropsy Evidence of Disseminated Intravascular Coagulation and Hemostatic Variables Before Death in Horses With Colic. J Vet Intern Med 2016 Jan-Feb;30(1):269-75.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
