Detection of myo-inositol tris pyrophosphate (ITPP) in equine following an administration of ITPP.
Abstract: Myo-Inositol tris pyrophosphate (ITPP) is a powerful allosteric modulator of haemoglobin that increases oxygen-releasing capacity of red blood cells. It is capable of crossing the red blood cell membrane unlike its open polyphosphate analog myo-inositol hexakisphosphate (IHP). Systemic administration of ITPP enhanced the exercise capacity in mice. There have been rumours of its abuse in the horse racing industry to enhance the performance of racing horses. In this paper, the detection of ITPP in equine plasma and urine after an administration of ITPP is reported. A Standardbred mare was administered 200 mg of ITPP intravenously. Urine and plasma samples were collected up to 120 h post administration and analyzed for ITPP by liquid chromatography-tandem mass spectrometry. ITPP was detected in post administration plasma samples up to 6 hours. The peak concentration was detected at 5 min post administration. In urine, ITPP was detected up to 24 h post administration. The peak concentration was detected at 1.5 h post administration.
Copyright © Her Majesty the Queen in Right of Canada 2013.
Publication Date: 2013-06-04 PubMed ID: 23733541DOI: 10.1002/dta.1473Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Validation Study
Summary
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The research article presents a study on the detection of myo-Inositol tris pyrophosphate (ITPP) in horse plasma and urine after being administered ITPP. Its main focus is on the potential misuse of ITPP in the horse racing industry to boost the performance of racing horses.
Objective and Background of the Study
- The main objective of this study was to investigate the presence and detectability of Myo-Inositol tris pyrophosphate (ITPP) in the body of a horse after it has been administered through an intravenous route.
- ITPP is an allosteric modulator of hemoglobin, capable of enhancing the oxygen-releasing capacity of red blood cells.
- Unlike its open polyphosphate analog, myo-inositol hexakisphosphate (IHP), ITPP can cross the red blood cell membrane.
- The focus on ITPP is due to rumors of its misuse in the horse racing industry, where it is potentially being used as a performance-enhancing substance.
Methodology
- A Standardbred mare was selected for the experiment, administered an intravenous dose of 200 mg of ITPP.
- Urine and plasma samples from the horse were collected up to 120 hours following ITPP administration.
- The collected samples were analyzed for the presence of ITPP using a method known as liquid chromatography-tandem mass spectrometry.
Findings
- ITPP was detected in the plasma samples post-administration for up to 6 hours. The peak concentration was observed at 5 minutes post-administration.
- In terms of urine samples, ITPP was identified up to 24 hours after its administration. The peak concentration, in this case, was noted at 1.5 hours post-administration.
Conclusion
- The results of the study demonstrate the detectability of ITPP in a horse’s body after it has been administered, supporting the rumor about its potential misuse as a performance-enhancing substance in the horse racing industry.
Cite This Article
APA
Lam G, Zhao S, Sandhu J, Yi R, Loganathan D, Morrissey B.
(2013).
Detection of myo-inositol tris pyrophosphate (ITPP) in equine following an administration of ITPP.
Drug Test Anal, 6(3), 268-276.
https://doi.org/10.1002/dta.1473 Publication
Researcher Affiliations
- Maxxam Analytics Inc., 8577 Commerce Court, Burnaby, BC, V5A 4N5, Canada.
MeSH Terms
- Animals
- Chromatography, High Pressure Liquid / methods
- Horses / blood
- Horses / urine
- Inositol Phosphates / administration & dosage
- Inositol Phosphates / blood
- Inositol Phosphates / urine
- Limit of Detection
- Substance Abuse Detection / methods
- Tandem Mass Spectrometry / methods
Citations
This article has been cited 2 times.- Bazzano M, Laghi L, Zhu C, Lotito E, Sgariglia S, Tesei B, Laus F. Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by (1)H-NMR.. Metabolites 2020 Jul 21;10(7).
- Bazzano M, Laghi L, Zhu C, Magi GE, Tesei B, Laus F. Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses.. BMC Vet Res 2020 Jul 8;16(1):233.
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