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Journal of dairy science2016; 99(4); 2480-2487; doi: 10.3168/jds.2015-9717

Determination of amino acid profile of mare milk produced in the highlands of the Kyrgyz Republic during the milking season.

Abstract: This study was carried out to determine the influence of milking season on amino acid (AA) profile and chemical composition of milk samples from Kyrgyz native breed mares under traditional pasture conditions. Milk samples were collected monthly from May to August 2014 from mares grazing at 2,200 m above sea level. The AA composition was determined by precolumn derivatization with diethyl ethoxymethylenemalonate in HPLC and 18 AA were determined in mare milk. The analytical parameters of linearity (0.01-4 μg/mL), precision of the method (0.26-4.88% relative standard deviation), derivatization procedure (0.82-2.80% relative standard deviation), and instrument precision (0.08-1.00% relative standard deviation) were determined. The most abundant AA were glutamic acid (0.393-480 g/100g of milk), leucine (0.192-0.230 g/100g of milk), and lysine (0.192-0.230 g/100g of milk). The amount of ornithine was small (0.002-0.015 g/100g of milk). The percentages of essential AA in the protein of mare milk were 46, 46, 51, and 48% for May, June, July, and August, respectively, which demonstrate the high biological value of milk during the whole milking season.
Publication Date: 2016-02-03 PubMed ID: 26851855DOI: 10.3168/jds.2015-9717Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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The research paper investigates the effect of the milking season on the amino acid profile and chemical composition of milk produced by the Kyrgyz native breed mares, raised under traditional pasture conditions in the Kyrgyz Republic’s highlands.

Research Methodology

  • The research collected milk samples monthly from May to August in 2014. The mares, from whose milk was being collected, were grazing at an altitude of 2200 meters above sea level.
  • The procedure to evaluate the composition of amino acids incorporated diethyl ethoxymethylenemalonate in High-Performance Liquid Chromatography (HPLC).
  • Eighteen different amino acids were determined in the mare milk through this method.
  • To validate the analytical process, the researchers also evaluated various parameters such as linearity, precision of the method, the procedure for derivatization, and instrumental precision.

Findings

  • The outcomes suggest that the most abundant amino acids present in mare milk were glutamic acid, leucine, and lysine. Ornithine was found in much smaller quantities.
  • The study observed the percentage of essential amino acids in the mare milk protein for each month of the sampling period.
  • The results found that the percentages for the months of May, June, July, and August were 46%, 46%, 51%, and 48%, respectively.

Conclusions

  • The proportions of essential amino acids consistently remained high throughout the milking season, highlighting the considerable nutritional value of mare milk during this period.
  • The study emphasizes the significance of traditional pasture conditions for maintaining mare milk’s quality and might be beneficial to those investigating adaptable milk production practices.

Cite This Article

APA
Mazhitova AT, Kulmyrzaev AA. (2016). Determination of amino acid profile of mare milk produced in the highlands of the Kyrgyz Republic during the milking season. J Dairy Sci, 99(4), 2480-2487. https://doi.org/10.3168/jds.2015-9717

Publication

ISSN: 1525-3198
NlmUniqueID: 2985126R
Country: United States
Language: English
Volume: 99
Issue: 4
Pages: 2480-2487
PII: S0022-0302(16)00110-7

Researcher Affiliations

Mazhitova, A T
  • Department of Food Engineering, Kyrgyz-Turkish Manas University, Bishkek 720038, Kyrgyzstan. Electronic address: mazhitova.aichurok@mail.ru.
Kulmyrzaev, A A
  • Department of Food Engineering, Kyrgyz-Turkish Manas University, Bishkek 720038, Kyrgyzstan.

MeSH Terms

  • Amino Acids / analysis
  • Animals
  • Female
  • Horses / metabolism
  • Kyrgyzstan
  • Milk / chemistry
  • Milk / metabolism
  • Seasons

Citations

This article has been cited 3 times.
  1. Meng Z, Zhou D, Lv D, Gan Q, Liao Y, Peng Z, Zhou X, Xu S, Chi P, Wang Z, Nüssler AK, Yang X, Liu L, Deng D, Yang W. Human milk extracellular vesicles enhance muscle growth and physical performance of immature mice associating with Akt/mTOR/p70s6k signaling pathway.. J Nanobiotechnology 2023 Aug 29;21(1):304.
    doi: 10.1186/s12951-023-02043-6pubmed: 37644475google scholar: lookup
  2. Barłowska J, Polak G, Janczarek I, Tkaczyk E. The Influence of Selected Factors on the Nutritional Value of the Milk of Cold-Blooded Mares: The Example of the Sokólski Breed.. Animals (Basel) 2023 Mar 24;13(7).
    doi: 10.3390/ani13071152pubmed: 37048410google scholar: lookup
  3. Nie P, Pan B, Ahmad MJ, Zhang X, Chen C, Yao Z, Lv H, Wei K, Yang L. Summer Buffalo Milk Produced in China: A Desirable Diet Enriched in Polyunsaturated Fatty Acids and Amino Acids.. Foods 2022 Nov 2;11(21).
    doi: 10.3390/foods11213475pubmed: 36360088google scholar: lookup