Development and validation of a method for Cd, Pb and As analysis in bovine, equine and poultry liver by inductively coupled plasma mass spectrometry.
Abstract: A method for the determination of As, Cd and Pb in bovine, equine and poultry liver by ICP-MS was developed and validated. Samples were digested in a microwave oven using a 10% HNO(3) solution. A set of experiments was made according to a central composite design (CCD) for optimisation of the plasma argon flow, nebuliser argon flow and radiofrequency power applied to the plasma. During the validation, Rh and Ru were evaluated as internal standards and, after validation, the best was Rh for Pb and Cd analysis, but for As better results were obtained without an internal standard. The method allowed As, Cd and Pb determination with a 3.3% HNO(3) solution for the calibration curves ranging from 0 to 40 µg l(-1) for As and from 0 to 20 µg l(-1) for Cd and Pb. The recovery values obtained showed averages of 100%, 106% and 96% for As, Cd and Pb, respectively. Limits of quantification obtained were 85 µg kg(-1) for As, 6.5 µg kg(-1) for Cd and 12.5 µg kg(-1) for Pb. Repeatability and within-laboratory reproducibility were evaluated through the indicators HORRAT(r) and HORRAT(R), and the results were less than 0.30. The method is simple, fast and showed adequate precision and accuracy for the determination of As, Cd and Pb in bovine, equine and poultry liver. The precision, recovery, uncertainties, and limits of detection and quantification for each analyte were in accordance to European Union Commission Regulation 2007/333/EC.
Publication Date: 2011-10-14 PubMed ID: 21995839DOI: 10.1080/19440049.2011.608381Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Validation Study
Summary
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The research article outlines the development and validation of a method using inductively coupled plasma mass spectrometry (ICP-MS) to measure the levels of arsenic (As), cadmium (Cd), and lead (Pb) in the liver tissue of various animals, including cattle, horses, and poultry.
Method Development
- The study introduced a new method to measure levels of As, Cd, and Pb in the livers of bovines, equines, and poultry using inductively coupled plasma mass spectrometry (ICP-MS).
- The liver samples were digested in a microwave oven using a 10% nitric acid (HNO3) solution to prepare for analysis.
- The researchers then optimized various parameters of the ICP-MS process. These adjustments were based on a central composite design (CCD) and include plasma argon flow, nebulizer argon flow, and the radio frequency power applied to the plasma.
Method Validation
- The method was validated by evaluating Rhodium (Rh) and Ruthenium (Ru) as internal standards for the analysis.
- Rhodium proved to be the best for Pb and Cd analysis, but for As, better results were achieved without an internal standard.
- The method allowed determination of As, Cd, and Pb with a 3.3% HNO3 solution permitting calibration curves ranging from 0 to 40 µg l(-1) for As and from 0 to 20 µg l(-1) for Cd and Pb.
Testing and Results
- The recovery values for As, Cd, and Pb were 100%, 106%, and 96% respectively, showing that the method effectively recovers these elements in the tested samples.
- The limits of quantification (the smallest concentration of analyte that can be reliably measured) for As, Cd, and Pb were 85 µg kg(-1), 6.5 µg kg(-1), and 12.5 µg kg(-1) respectively.
- Repeatability and within-laboratory reproducibility of the method were also tested. The metrics HORRAT(r) and HORRAT(R) were used for this, with values less than 0.30 indicating good repeatability and reproducibility.
Conclusion
- The developed method is simple, fast, and has adequate precision and accuracy for the determination of As, Cd, and Pb in bovine, equine, and poultry liver.
- The method aligns with the European Union Commission Regulation 2007/333/EC concerning the precision, recovery, uncertainties, and limits of detection and quantification for each analyte.
Cite This Article
APA
Lara PC, Fabrino HJ, Germano A, Silva JB.
(2011).
Development and validation of a method for Cd, Pb and As analysis in bovine, equine and poultry liver by inductively coupled plasma mass spectrometry.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 29(4), 609-616.
https://doi.org/10.1080/19440049.2011.608381 Publication
Researcher Affiliations
- Ministry of Agriculture, Livestock and Supply - National Agricultural Laboratory, Pedro Leopoldo, MG, Brazil. paulocpl@gmail.com
MeSH Terms
- Animals
- Arsenic / analysis
- Brazil
- Cadmium / analysis
- Cattle
- Food Contamination / analysis
- Food Contamination / legislation & jurisprudence
- Food Contamination / prevention & control
- Food Contamination / statistics & numerical data
- Horses
- Lead / analysis
- Limit of Detection
- Liver / chemistry
- Mass Spectrometry / methods
- Meat / analysis
- Poultry
Citations
This article has been cited 1 times.- Barrasso R, Ceci E, Stinga L, Tantillo G, Bozzo G. Presence of cadmium residues in muscle, liver and kidney of Bubalus bubalis and histological evidence.. Ital J Food Saf 2018 Sep 26;7(3):7684.
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