Development of a multi-residue high-throughput UHPLC-MS/MS method for routine monitoring of SARM compounds in equine and bovine blood.
Abstract: Selective androgen receptor modulators (SARMs) are a group of anabolic enhancer drugs posing threats to the integrity of animal sports and the safety of animal-derived foods. The current research describes for the first time the development of a semi-quantitative assay for the monitoring of SARM family compounds in blood and establishes the relative stability of these analytes under various storage conditions prior to analysis. The presented screening method validation was performed in line with current EU legislation for the inspection of livestock and produce of animal origin, with detection capability (CCβ) values determined at 0.5 ng/mL (Ly2452473), 1 ng/mL (AC-262536 and PF-06260414), 2 ng/mL (bicalutamide, GLPG0492, LGD-2226, ostarine, S-1, S-6, and S-23), and 5 ng/mL (andarine, BMS-564929, LGD-4033, RAD140, and S-9), respectively. The applicability of the developed assay was demonstrated through the analysis of blood samples from racehorses and cattle. The developed method presents a high-throughput cost-effective tool for the routine screening for a range of SARM compounds in sport and livestock animals.
© 2020 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
Publication Date: 2020-06-24 PubMed ID: 32519780DOI: 10.1002/dta.2875Google Scholar: Lookup
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- Journal Article
- Validation Study
Summary
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The research article presents a new semi-quantitative method for detecting Selective androgen receptor modulators (SARMs)—a type of performance-enhancing drug—in the blood of racehorses and cattle. This method was validated in accordance with EU regulations and provides an efficient, cost-effective tool to maintain integrity in animal sports and food safety.
Development and Validation of New Method
- This research introduces a novel method for monitoring the presence of SARMs in animal blood. SARMs are drugs used to enhance physical performance and pose risks to the integrity of sports involving animals. Their presence in animals also threatens the safety of food derived from these animals.
- The new assay is semi-quantitative and has been developed specifically for the routine monitoring of various SARM compounds found in the blood.
Verification of the Method
- In this study, the effectiveness of the method was demonstrated by analyzing blood samples taken from racehorses and cattle. This provided practical evidence of the method’s applicability.
- The validity of the screening method was assessed according to the existing EU legislation set out for the inspection of livestock and produce of animal origin. This ensures that the method meets the required industry standards.
Assessment of Compound Stability
- In addition to developing the method, the study also examined the stability of the SARM compounds under various storage conditions prior to analysis. Understanding of the stability of these compounds is crucial to ensure accurate and reliable results.
Appearance and Usability of the New Method
- The developed method offers an efficient, cost-effective solution for routinely screening a range of SARM compounds in animals involved in sports and livestock.
- Detection capability values for the various compounds were determined at levels ranging from 0.5 ng/mL to 5 ng/mL. These figures indicate the sensitivity and precision of the developed method.
Cite This Article
APA
Ventura E, Gadaj A, Buckley T, Mooney MH.
(2020).
Development of a multi-residue high-throughput UHPLC-MS/MS method for routine monitoring of SARM compounds in equine and bovine blood.
Drug Test Anal, 12(9), 1373-1379.
https://doi.org/10.1002/dta.2875 Publication
Researcher Affiliations
- Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, UK.
- Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, UK.
- Irish Diagnostic Laboratory Services Ltd, Johnstown, Co. Kildare, Ireland.
- Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, UK.
MeSH Terms
- Anabolic Agents / analysis
- Anabolic Agents / blood
- Androgens / analysis
- Androgens / blood
- Animals
- Cattle
- Chromatography, High Pressure Liquid / economics
- Chromatography, High Pressure Liquid / methods
- Cost-Benefit Analysis
- Doping in Sports
- Drug Stability
- Drug Storage
- High-Throughput Screening Assays / economics
- Horses
- Substance Abuse Detection / economics
- Substance Abuse Detection / methods
- Tandem Mass Spectrometry / economics
- Tandem Mass Spectrometry / methods
Grant Funding
- 642380 / European Union's Horizon 2020 research and innovation programme under the Marie Sku0142odowska-Curie
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This article includes 20 references
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Citations
This article has been cited 1 times.- Benedetto A, Biasibetti E, Robotti E, Marengo E, Audino V, Bozzetta E, Pezzolato M. Transcriptional Biomarkers and Immunohistochemistry for Detection of Illicit Dexamethasone Administration in Veal Calves.. Foods 2022 Jun 20;11(12).
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