Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens.
Abstract: The prevalence of equine piroplasmosis in Xinjiang province, China, was examined by enzyme-linked immunosorbent assays (ELISAs). A total of 70 serum samples were taken from horses pastured on three farms in western Xinjiang, and examined for diagnosis of equine Babesia equi (B. equi) infection and B. caballi infection by ELISAs using recombinant equi merozoite antigen 1 (EMA-1) and recombinant P48 antigen, respectively. Of the 70 samples, 28 (40.0%) and 17 (24.3%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 11 (15.7%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in western Xinjiang. To our knowledge, this is the first report describing a survey on equine piroplasmosis in Xinjiang province, China.
Publication Date: 2002-09-05 PubMed ID: 12208045DOI: 10.1016/s0304-4017(02)00193-0Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Diagnosis
- Disease Diagnosis
- Disease Management
- Disease Prevalence
- Disease Surveillance
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Horses
- Infection
- Infectious Disease
- Piroplasmosis
- Public Health
- Serodiagnosis
- Seroprevalence
- Theileria equi
- Veterinary Medicine
- Veterinary Research
- Veterinary Science
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study examines the prevalence of equine piroplasmosis, a disease in horses transmitted by ticks, in the Xinjiang province of China, using enzyme-linked immunosorbent assays (ELISAs). The study finds that the disease is widespread in the region.
Objective and Methods
- The research aimed to understand the prevalence of equine piroplasmosis in the Xinjiang province of China.
- The researchers used the enzyme-linked immunosorbent assays (ELISAs) technique which is a popular lab test used to measure the concentration of antibodies, antigens, proteins, and glycoproteins in biological samples.
- ELISAs were implemented using recombinant equi merozoite antigen 1 (EMA-1) and recombinant P48 antigen to diagnose equine Babesia equi (B. equi) infection and B. caballi infection respectively.
- Recombinant antigens are proteins produced through recombinant DNA technology, which manipulate organism’s gene order and spaces.
- A total of 70 serum samples were collected from horses pastured on three different farms in the western part of Xinjiang province.
Findings
- Out of the 70 samples collected, 28 samples (40.0%) tested positive for B. equi infection, and 17 samples (24.3%) tested positive for B. caballi infection.
- Moreover, there were 11 samples (15.7%) that tested positive for both B. equi and B. caballi infections.
- The results suggested a widespread prevalence of equine piroplasmosis in the studied region, making it a serious concern.
- This study is significant and novel as it represents the first report describing a survey of equine piroplasmosis prevalence in Xinjiang province, China.
Cite This Article
APA
Xuan X, Chahan B, Huang X, Yokoyama N, Makala LH, Igarashi I, Fujisaki K, Maruyama S, Sakai T, Mikami T.
(2002).
Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens.
Vet Parasitol, 108(2), 179-182.
https://doi.org/10.1016/s0304-4017(02)00193-0 Publication
Researcher Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. gen@obihiro.ac.jp
MeSH Terms
- Animals
- Antibodies, Protozoan / blood
- Antigens, Protozoan
- Babesia / isolation & purification
- Babesiosis / diagnosis
- Babesiosis / epidemiology
- Babesiosis / parasitology
- Babesiosis / veterinary
- China / epidemiology
- Enzyme-Linked Immunosorbent Assay / methods
- Enzyme-Linked Immunosorbent Assay / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / epidemiology
- Horse Diseases / parasitology
- Horses
- Prevalence
- Recombinant Proteins
Citations
This article has been cited 5 times.- Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny.. Pathogens 2020 Nov 8;9(11).
- Montes Cortés MG, Fernández-García JL, Habela Martínez-Estéllez MÁ. Seroprevalence of Theileria equi and Babesia caballi in horses in Spain.. Parasite 2017;24:14.
- Chen Z, Liu Q, Jiao FC, Xu BL, Zhou XN. Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China.. Infect Dis Poverty 2014;3:18.
- Wang M, Guo W, Igarashi I, Xuan X, Wang X, Xiang W, Jia H. Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays.. J Vet Med Sci 2014 Apr;76(4):549-52.
- Jaffer O, Abdishakur F, Hakimuddin F, Riya A, Wernery U, Schuster RK. A comparative study of serological tests and PCR for the diagnosis of equine piroplasmosis.. Parasitol Res 2010 Feb;106(3):709-13.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
