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Journal of animal science2019; 97(12); 4951-4956; doi: 10.1093/jas/skz330

Dietary tributyrin supplementation and submaximal exercise promote activation of equine satellite cells.

Abstract: Postexercise skeletal muscle repair is dependent on the actions of satellite cells (SCs). The signal(s) responsible for activation of these normally quiescent cells in the horse remain unknown. The objective of the experiment was to determine whether submaximal exercise or tributyrin (TB) supplementation is sufficient to stimulate SC activation. Adult geldings were fed a control diet (n = 6) or a diet containing 0.45% TB (n = 6). After 30 d, the geldings performed a single bout of submaximal exercise. Middle gluteal muscle biopsies and blood were collected on days -1, 1, 3, and 5 relative to exercise. Diet had no effect on any parameter of physical performance. Total RNA isolated from the gluteal muscle of TB fed geldings contained greater (P < 0.05) amounts of myogenin mRNA than controls. Satellite cell isolates from TB supplemented horses had a greater (P = 0.02) percentage of proliferating cell nuclear antigen immunopositive (PCNA+) SC than controls after 48 h in culture. Submaximal exercise was sufficient to increase (P < 0.05) the percentage of PCNA(+) cells in all isolates obtained during recovery period. No change in the amount of gluteal muscle Pax7 mRNA, a lineage marker of SCs, occurred in response to either diet or exercise. Our results indicate that both submaximal exercise and TB prime SCs for activation and cell cycle reentry but are insufficient to cause an increase in Pax7 expression during the recovery period.
© The Author(s) 2019. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
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Publication Date: 2019-10-21 PubMed ID: 31630180PubMed Central: PMC6915229DOI: 10.1093/jas/skz330Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores how a dietary additive, Tributyrin (TB), and submaximal exercise severity can influence the activation of satellite cells (SCs) – crucial entities in skeletal muscle repair – in horses.

Objective and Methodology

The research was aimed to investigate if submaximal exercise and TB supplementation can stimulate the activation of SCs, which are typically inactive in horses. In the matter of methodology, adult geldings (male horses) were divided into two groups. One group was fed a control diet whereas the other received a diet containing 0.45% TB. After a period of 30 days, the horses performed a single bout of submaximal exercise. Muscle biopsies and blood samples were collected from the horses on day -1, 1, 3, and 5 relative to exercise.

  • A total RNA was isolated from the gluteal muscle of horses supplemented with TB.
  • SC isolates from horses supplemented with TB were tested for percentage of proliferating cell nuclear antigen immunopositive (PCNA+),a marker of dividing cells.
  • Submaximal exercise’s effects on the percentage of PCNA(+) cells were evaluated.
  • They also checked for any changes in the quantity of Pax7 mRNA, an identifier of SCs, in response to diet or exercise.

Findings

The research discovered that feeding horses with a diet supplemented with TB or exposing them to submaximal exercise does not impact their physical performance parameters. However, there were significantly higher levels of myogenin mRNA in the total RNA isolated from the gluteal muscle of TB-fed horses compared to the control group.

  • The SC isolates obtained from TB-supplemented horses displayed a greater percentage of PCNA+ SCs after 48 hours in culture when compared to those from control diet-fed horses.
  • Submaximal exercise was sufficient to augment the percentage of PCNA(+) cells in all the isolates obtained during the recovery phase.
  • Neither the TB dietary supplement nor the submaximal exercise resulted in any change in the levels of Pax7 mRNA, which is a lineage marker of SCs.

Conclusion

In conclusion, both submaximal exercise and a diet supplemented with TB were found to facilitate the activation and cell cycle re-entry of SCs, though these interventions were not sufficient to cause an increase in Pax7 expression during the recovery period.

Cite This Article

APA
Gonzalez ML, Jacobs RD, Ely KM, Johnson SE. (2019). Dietary tributyrin supplementation and submaximal exercise promote activation of equine satellite cells. J Anim Sci, 97(12), 4951-4956. https://doi.org/10.1093/jas/skz330

Publication

Journal of animal science
ISSN: 1525-3163
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 97
Issue: 12
Pages: 4951-4956

Researcher Affiliations

Gonzalez, Madison L
  • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA.
Jacobs, Robert D
  • Purina Animal Nutrition, Gray Summit, MO.
Ely, Kristine M
  • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA.
Johnson, Sally E
  • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA.

MeSH Terms

  • Animal Feed
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Diet / veterinary
  • Dietary Supplements
  • Horses / physiology
  • Male
  • Muscle, Skeletal / physiology
  • Myogenin
  • PAX7 Transcription Factor / genetics
  • PAX7 Transcription Factor / metabolism
  • Physical Conditioning, Animal / physiology
  • Proliferating Cell Nuclear Antigen / metabolism
  • RNA, Messenger / metabolism
  • Satellite Cells, Skeletal Muscle / physiology
  • Triglycerides / administration & dosage
  • Triglycerides / pharmacology

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Citations

This article has been cited 3 times.
  1. Dang X, Lee H, Lee SJ, Song JH, Mun S, Lee KY, Han K, Kim IH. Tributyrin and anise mixture supplementation improves growth performance, nutrient digestibility, jejunal villus height, and fecal microbiota in weaned pigs. Front Vet Sci 2023;10:1107149.
    doi: 10.3389/fvets.2023.1107149pubmed: 36777676google scholar: lookup
  2. Busse NI, Gonzalez ML, Wagner AL, Johnson SE. Short Communication: Supplementation with calcium butyrate causes an increase in the percentage of oxidative fibers in equine gluteus medius muscle. J Anim Sci 2022 Aug 1;100(8).
    doi: 10.1093/jas/skac108pubmed: 35908781google scholar: lookup
  3. Gregg SR, Barshick MR, Johnson SE. Intravenous Injection of Sodium Hyaluronate Diminishes Basal Inflammatory Gene Expression in Equine Skeletal Muscle. Animals (Basel) 2023 Sep 27;13(19).
    doi: 10.3390/ani13193030pubmed: 37835636google scholar: lookup
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