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Home / Equine Research Bank / Histochem Cell Biol / Differing in vitro biology of equine, ovine, porcine and hum...
Histochemistry and cell biology2008; 131(2); 219-229; doi: 10.1007/s00418-008-0516-6

Differing in vitro biology of equine, ovine, porcine and human articular chondrocytes derived from the knee joint: an immunomorphological study.

Abstract: For lack of sufficient human cartilage donors, chondrocytes isolated from various animal species are used for cartilage tissue engineering. The present study was undertaken to compare key features of cultured large animal and human articular chondrocytes of the knee joint. Primary chondrocytes were isolated from human, porcine, ovine and equine full thickness knee joint cartilage and investigated flow cytometrically for their proliferation rate. Synthesis of extracellular matrix proteins collagen type II, cartilage proteoglycans, collagen type I, fibronectin and cytoskeletal organization were studied in freshly isolated or passaged chondrocytes using immunohistochemistry and western blotting. Chondrocytes morphology, proliferation, extracellular matrix synthesis and cytoskeleton assembly differed substantially between these species. Proliferation was higher in animal derived compared with human chondrocytes. All chondrocytes expressed a cartilage-specific extracellular matrix. However, after monolayer expansion, cartilage proteoglycan expression was barely detectable in equine chondrocytes whereby fibronectin and collagen type I deposition increased compared with porcine and human chondrocytes. Animal-derived chondrocytes developed more F-actin fibers during culturing than human chondrocytes. With respect to proliferation and extracellular matrix synthesis, human chondrocytes shared more similarity with porcine than with ovine or equine chondrocytes. These interspecies differences in chondrocytes in vitro biology should be considered when using animal models.
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Publication Date: 2008-10-07 PubMed ID: 18839203DOI: 10.1007/s00418-008-0516-6Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the differences in characteristics of knee joint chondrocytes (cells that make up cartilage) taken from humans and various animals such as pigs, sheep, and horses. The findings reveal that though all chondrocytes produced cartilage-specific external matrix, there were notable variations in proliferation rate, morphological appearance and the synthesis of extracellular matrix proteins between the species.

Research Methodology

  • The research involved the isolation of primary chondrocytes from full thickness knee joint cartilage of humans, pigs, sheep, and horses.
  • The proliferation rate of these chondrocytes, or their rate of growth and reproduction, were then studied using flow cytometry.
  • The study further investigated the synthesis of extracellular matrix proteins such as collagen type II, cartilage proteoglycans, collagen type I, and fibronectin. This was done both in freshly isolated or passaged chondrocytes using immunohistochemistry and western blotting methods.
  • The morphology or the form and structure of chondrocytes and the cytoskeleton assembly were also carefully observed and analyzed.

Main Findings

  • The study found that the cell replication or proliferation rate was higher in animal derived chondrocytes than in human chondrocytes.
  • Although all chondrocytes expressed a cartilage-specific extracellular matrix, there were differences noted after monolayer expansion. Specifically, the study observed that the expression of cartilage proteoglycan was barely detectable in chondrocytes derived from horses.
  • Furthermore, the researchers found that there was an enhanced deposition of fibronectin and collagen type I in horse chondrocytes when compared with those derived from pigs and humans.
  • The study also observed that the animal-derived chondrocytes developed more F-actin fibers during the culture process compared to human chondrocytes.
  • In terms of proliferation and extracellular matrix synthesis, human chondrocytes were found to have more resemblances with pig chondrocytes than with sheep or horse chondrocytes.

Implications of the Study

  • The research suggests that these significant interspecies differences in chondrocytes’ in vitro biology should be taken into account when using animal models in cartilage tissue engineering or related studies.
  • In particular, the lack of sufficient human cartilage donors makes this study significant as it provides insights into the feasibility and challenges of using chondrocytes isolated from different animal species.

Cite This Article

APA
Schulze-Tanzil G, Müller RD, Kohl B, Schneider N, Ertel W, Ipaktchi K, Hünigen H, Gemeinhardt O, Stark R, John T. (2008). Differing in vitro biology of equine, ovine, porcine and human articular chondrocytes derived from the knee joint: an immunomorphological study. Histochem Cell Biol, 131(2), 219-229. https://doi.org/10.1007/s00418-008-0516-6

Publication

Histochemistry and cell biology
ISSN: 1432-119X
NlmUniqueID: 9506663
Country: Germany
Language: English
Volume: 131
Issue: 2
Pages: 219-229

Researcher Affiliations

Schulze-Tanzil, G
  • Department of Trauma and Reconstructive Surgery, Charité-Campus Benjamin Franklin, FEM, Krahmerstrasse 6-10, 12207, Berlin, Germany. gundula.schulze-tanzil@charite.de
Müller, R D
    Kohl, B
      Schneider, N
        Ertel, W
          Ipaktchi, K
            Hünigen, H
              Gemeinhardt, O
                Stark, R
                  John, T

                    MeSH Terms

                    • Actins / analysis
                    • Animals
                    • Cartilage, Articular / cytology
                    • Cell Proliferation
                    • Cells, Cultured
                    • Chondrocytes / chemistry
                    • Chondrocytes / cytology
                    • Chondrocytes / metabolism
                    • Cytoskeleton
                    • Extracellular Matrix Proteins / analysis
                    • Horses
                    • Humans
                    • Knee Joint
                    • Sheep
                    • Species Specificity
                    • Swine

                    References

                    This article includes 23 references
                    1. Martin I, Vunjak-Novakovic G, Yang J, Langer R, Freed LE. Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue.. Exp Cell Res 1999 Dec 15;253(2):681-8.
                      pubmed: 10585291doi: 10.1006/excr.1999.4708google scholar: lookup
                    2. Little CB, Ghosh P. Variation in proteoglycan metabolism by articular chondrocytes in different joint regions is determined by post-natal mechanical loading.. Osteoarthritis Cartilage 1997 Jan;5(1):49-62.
                      pubmed: 9010878doi: 10.1016/s1063-4584(97)80031-3google scholar: lookup
                    3. Benya PD, Shaffer JD. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels.. Cell 1982 Aug;30(1):215-24.
                      pubmed: 7127471doi: 10.1016/0092-8674(82)90027-7google scholar: lookup
                    4. Archer CW, Francis-West P. The chondrocyte.. Int J Biochem Cell Biol 2003 Apr;35(4):401-4.
                      pubmed: 12565700doi: 10.1016/s1357-2725(02)00301-1google scholar: lookup
                    5. Hamilton DW, Riehle MO, Monaghan W, Curtis AS. Articular chondrocyte passage number: influence on adhesion, migration, cytoskeletal organisation and phenotype in response to nano- and micro-metric topography.. Cell Biol Int 2005 Jun;29(6):408-21.
                      pubmed: 15979350doi: 10.1016/j.cellbi.2004.12.008google scholar: lookup
                    6. Schulze-Tanzil G, de Souza P, Villegas Castrejon H, John T, Merker HJ, Scheid A, Shakibaei M. Redifferentiation of dedifferentiated human chondrocytes in high-density cultures.. Cell Tissue Res 2002 Jun;308(3):371-9.
                      pubmed: 12107430doi: 10.1007/s00441-002-0562-7google scholar: lookup
                    7. Knudson W, Loeser RF. CD44 and integrin matrix receptors participate in cartilage homeostasis.. Cell Mol Life Sci 2002 Jan;59(1):36-44.
                      pubmed: 11846031doi: 10.1007/s00018-002-8403-0google scholar: lookup
                    8. Thomas CM, Fuller CJ, Whittles CE, Sharif M. Chondrocyte death by apoptosis is associated with cartilage matrix degradation.. Osteoarthritis Cartilage 2007 Jan;15(1):27-34.
                      pubmed: 16859932doi: 10.1016/j.joca.2006.06.012google scholar: lookup
                    9. Schulze-Tanzil G, Mobasheri A, de Souza P, John T, Shakibaei M. Loss of chondrogenic potential in dedifferentiated chondrocytes correlates with deficient Shc-Erk interaction and apoptosis.. Osteoarthritis Cartilage 2004 Jun;12(6):448-58.
                      pubmed: 15135141doi: 10.1016/j.joca.2004.02.007google scholar: lookup
                    10. Peretti GM, Xu JW, Bonassar LJ, Kirchhoff CH, Yaremchuk MJ, Randolph MA. Review of injectable cartilage engineering using fibrin gel in mice and swine models.. Tissue Eng 2006 May;12(5):1151-68.
                      pubmed: 16771631doi: 10.1089/ten.2006.12.1151google scholar: lookup
                    11. Tran-Khanh N, Hoemann CD, McKee MD, Henderson JE, Buschmann MD. Aged bovine chondrocytes display a diminished capacity to produce a collagen-rich, mechanically functional cartilage extracellular matrix.. J Orthop Res 2005 Nov;23(6):1354-62.
                      pubmed: 16048738doi: 10.1016/j.orthres.2005.05.009.1100230617google scholar: lookup
                    12. Zaidel-Bar R, Cohen M, Addadi L, Geiger B. Hierarchical assembly of cell-matrix adhesion complexes.. Biochem Soc Trans 2004 Jun;32(Pt3):416-20.
                      pubmed: 15157150doi: 10.1042/BST0320416google scholar: lookup
                    13. Vittur F, Grandolfo M, Fragonas E, Godeas C, Paoletti S, Pollesello P, Kvam BJ, Ruzzier F, Starc T, Mozrzymas JW. Energy metabolism, replicative ability, intracellular calcium concentration, and ionic channels of horse articular chondrocytes.. Exp Cell Res 1994 Jan;210(1):130-6.
                      pubmed: 8269989doi: 10.1006/excr.1994.1019google scholar: lookup
                    14. Murray RC, Zhu CF, Goodship AE, Lakhani KH, Agrawal CM, Athanasiou KA. Exercise affects the mechanical properties and histological appearance of equine articular cartilage.. J Orthop Res 1999 Sep;17(5):725-31.
                      pubmed: 10569483doi: 10.1002/jor.1100170516google scholar: lookup
                    15. Akens MK, Hurtig MB. Influence of species and anatomical location on chondrocyte expansion.. BMC Musculoskelet Disord 2005 May 17;6:23.
                      pubmed: 15904515doi: 10.1186/1471-2474-6-23google scholar: lookup
                    16. Cao L, Lee V, Adams ME, Kiani C, Zhang Y, Hu W, Yang BB. beta-Integrin-collagen interaction reduces chondrocyte apoptosis.. Matrix Biol 1999 Aug;18(4):343-55.
                      pubmed: 10517181doi: 10.1016/s0945-053x(99)00027-xgoogle scholar: lookup
                    17. Giannoni P, Crovace A, Malpeli M, Maggi E, Arbicò R, Cancedda R, Dozin B. Species variability in the differentiation potential of in vitro-expanded articular chondrocytes restricts predictive studies on cartilage repair using animal models.. Tissue Eng 2005 Jan-Feb;11(1-2):237-48.
                      pubmed: 15738678doi: 10.1089/ten.2005.11.237google scholar: lookup
                    18. Yang C, Li SW, Helminen HJ, Khillan JS, Bao Y, Prockop DJ. Apoptosis of chondrocytes in transgenic mice lacking collagen II.. Exp Cell Res 1997 Sep 15;235(2):370-3.
                      pubmed: 9299161doi: 10.1006/excr.1997.3692google scholar: lookup
                    19. Kim HJ, Park SR, Park HJ, Choi BH, Min BH. Potential predictive markers for proliferative capacity of cultured human articular chondrocytes: PCNA and p21.. Artif Organs 2005 May;29(5):393-8.
                      pubmed: 15854215doi: 10.1111/j.1525-1594.2005.29066.xgoogle scholar: lookup
                    20. Todd Allen R, Robertson CM, Harwood FL, Sasho T, Williams SK, Pomerleau AC, Amiel D. Characterization of mature vs aged rabbit articular cartilage: analysis of cell density, apoptosis-related gene expression and mechanisms controlling chondrocyte apoptosis.. Osteoarthritis Cartilage 2004 Nov;12(11):917-23.
                      pubmed: 15501408doi: 10.1016/j.joca.2004.08.003google scholar: lookup
                    21. Zwicky R, Baici A. Cytoskeletal architecture and cathepsin B trafficking in human articular chondrocytes.. Histochem Cell Biol 2000 Nov;114(5):363-72.
                      pubmed: 11151406doi: 10.1007/s004180000199google scholar: lookup
                    22. Hirsch MS, Lunsford LE, Trinkaus-Randall V, Svoboda KK. Chondrocyte survival and differentiation in situ are integrin mediated.. Dev Dyn 1997 Nov;210(3):249-63.
                      pubmed: 9389451doi: 10.1002/(SICI)1097-0177(199711)210:3<249::AID-AJA6>3.0.CO;2-Ggoogle scholar: lookup
                    23. Dye SF. An evolutionary perspective of the knee.. J Bone Joint Surg Am 1987 Sep;69(7):976-83.
                      pubmed: 3654710

                    Citations

                    This article has been cited 11 times.
                    1. Damerau A, Gaber T. Modeling Rheumatoid Arthritis In Vitro: From Experimental Feasibility to Physiological Proximity.. Int J Mol Sci 2020 Oct 25;21(21).
                      doi: 10.3390/ijms21217916pubmed: 33113770google scholar: lookup
                    2. Žigon-Branc S, Barlič A, Knežević M, Jeras M, Vunjak-Novakovic G. Testing the potency of anti-TNF-α and anti-IL-1β drugs using spheroid cultures of human osteoarthritic chondrocytes and donor-matched chondrogenically differentiated mesenchymal stem cells.. Biotechnol Prog 2018 Jul;34(4):1045-1058.
                      doi: 10.1002/btpr.2629pubmed: 29536646google scholar: lookup
                    3. Badendick J, Godkin O, Kohl B, Meier C, Jagielski M, Huang Z, Arens S, Schneider T, Schulze-Tanzil G. Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage.. Clin Med Insights Arthritis Musculoskelet Disord 2016;9:65-74.
                      doi: 10.4137/CMAMD.S29844pubmed: 27158224google scholar: lookup
                    4. DuRaine GD, Brown WE, Hu JC, Athanasiou KA. Emergence of scaffold-free approaches for tissue engineering musculoskeletal cartilages.. Ann Biomed Eng 2015 Mar;43(3):543-54.
                      doi: 10.1007/s10439-014-1161-ypubmed: 25331099google scholar: lookup
                    5. Lehmann M, Martin F, Mannigel K, Kaltschmidt K, Sack U, Anderer U. Three-dimensional scaffold-free fusion culture: the way to enhance chondrogenesis of in vitro propagated human articular chondrocytes.. Eur J Histochem 2013 Nov 5;57(4):e31.
                      doi: 10.4081/ejh.2013.e31pubmed: 24441184google scholar: lookup
                    6. Zilkens C, Miese FR, Crumbiegel C, Kim YJ, Herten M, Antoch G, Krauspe R, Bittersohl B. Magnetic resonance imaging and histology of ovine hip joint cartilage in two age populations: a sheep model with assumed healthy cartilage.. Skeletal Radiol 2013 May;42(5):699-705.
                      doi: 10.1007/s00256-012-1554-7pubmed: 23275026google scholar: lookup
                    7. Nakamura T, Sekiya I, Muneta T, Hatsushika D, Horie M, Tsuji K, Kawarasaki T, Watanabe A, Hishikawa S, Fujimoto Y, Tanaka H, Kobayashi E. Arthroscopic, histological and MRI analyses of cartilage repair after a minimally invasive method of transplantation of allogeneic synovial mesenchymal stromal cells into cartilage defects in pigs.. Cytotherapy 2012 Mar;14(3):327-38.
                      doi: 10.3109/14653249.2011.638912pubmed: 22309371google scholar: lookup
                    8. Lohan A, Marzahn U, El Sayed K, Haisch A, Kohl B, Müller RD, Ertel W, Schulze-Tanzil G, John T. In vitro and in vivo neo-cartilage formation by heterotopic chondrocytes seeded on PGA scaffolds.. Histochem Cell Biol 2011 Jul;136(1):57-69.
                      doi: 10.1007/s00418-011-0822-2pubmed: 21656225google scholar: lookup
                    9. Cheng NC, Estes BT, Young TH, Guilak F. Engineered cartilage using primary chondrocytes cultured in a porous cartilage-derived matrix.. Regen Med 2011 Jan;6(1):81-93.
                      doi: 10.2217/rme.10.87pubmed: 21175289google scholar: lookup
                    10. Mancuso L, Liuzzo MI, Fadda S, Pisu M, Cincotti A, Arras M, La Nasa G, Concas A, Cao G. In vitro ovine articular chondrocyte proliferation: experiments and modelling.. Cell Prolif 2010 Jun;43(3):310-20.
                      doi: 10.1111/j.1365-2184.2010.00676.xpubmed: 20412130google scholar: lookup
                    11. Heupel WM, Drenckhahn D. Extending the knowledge in histochemistry and cell biology.. Histochem Cell Biol 2010 Jan;133(1):1-40.
                      doi: 10.1007/s00418-009-0665-2pubmed: 19946696google scholar: lookup
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