[Distribution of Borna disease virus in naturally infected animals with clinical disease].
Abstract: Borna disease (BD) is a naturally occurring enzootic encephalomyelitis of horses and sheep. The aetiological agent, Borna disease virus (BDV) is an unclassified, neurotropic, negative stranded RNA virus. The study aimed at providing further information on BD of naturally infected animals. Samples obtained from 20 animals (18 horses, 1 donkey, 1 sheep) were investigated by a series of virological and molecular biological tests. The highly sensitive reverse transcription-polymerase chain reaction (RT-PCR) method was used to analyze the tissue distribution of BDV-specific RNA. BDV-specific RNA was detected in bulbus olfactorius, nucleus caudatus, hippocampus and cerebral cortex of all infected animals. BDV-RNA was also present in the spinal cord, eye, nasal mucosa, parotide gland, lung, heart, liver, kidney, bladder and ovaries. In addition, BV-specific RNA was also detected in conjunctival fluid, nasal secretions and saliva of two infected animals. By Western Blot assays the highest amounts of BDV antigens were demonstrated in bulbus olfactorius, nucleus caudatus, hippocampus and cerebral cortex.
Publication Date: 1996-05-01 PubMed ID: 8694747
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- English Abstract
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study is about distribution of Borna disease Virus (BDV), a neurotropic, negative RNA virus affecting mainly horses and sheep. The researchers used highly sensitive techniques like RT-PCR to probe the distribution of BDV in various body tissues and fluids of infected animals.
Objective of the Study
- The study aimed to provide in-depth information about Borna Disease (BD) in naturally infected animals like horses and sheep and locating where the virus is situated.
Methods Used
- Specimens from twenty animals (18 horses, 1 donkey, 1 sheep) were gathered and analyzed using virological and molecular biological tests.
- Reverse Transcription Polymerase Chain Reaction (RT-PCR), a highly sensitive method, was employed for the investigation of the tissue distribution of virus-specific RNA.
Findings
- BDV-specific RNA was detected in different areas of the brain, such as the olfactory bulb, caudate nucleus, hippocampus, and cerebral cortex in all infected animals.
- The virus was also found in multiple body organ tissues such as the spinal cord, eyes, nasal mucosa, parotid gland, lung, heart, liver, kidney, bladder, and ovaries.
- Notably, BV-specific RNA was discovered in conjunctival fluid, nasal secretions, and saliva in some of the infected animals.
- Through Western Blot assays, another testing method, they were able to confirm the presence of the most BDV antigens – proteins that trigger an immune response – in the olfactory bulb, caudate nucleus, hippocampus, and cerebral cortex areas of the brain.
Significance of the Study
- The findings of this research provide valuable insights into the distribution and behavior of BDV in various tissues of infected animals.
- It will guide future research on the pathogenicity, mechanism of infection, and treatment methods for diseases caused by BDV.
Cite This Article
APA
Lebelt J, Hagenau K.
(1996).
[Distribution of Borna disease virus in naturally infected animals with clinical disease].
Berl Munch Tierarztl Wochenschr, 109(5), 178-183.
Publication
Researcher Affiliations
- Institut für Virologie, Veterinärmedizinische Fakultät, Universität Leipzig.
MeSH Terms
- Animals
- Borna Disease / pathology
- Borna disease virus / isolation & purification
- Brain / pathology
- DNA Probes
- Horse Diseases / pathology
- Horses
- Polymerase Chain Reaction
- RNA, Viral / analysis
- Sheep
- Sheep Diseases / pathology
- Tissue Distribution
Citations
This article has been cited 6 times.- More S, Bøtner A, Butterworth A, Calistri P, Depner K, Edwards S, Garin-Bastuji B, Good M, Gortázar Schmidt C, Michel V, Miranda MA, Nielsen SS, Raj M, Sihvonen L, Spoolder H, Stegeman JA, Thulke HH, Velarde A, Willeberg P, Winckler C, Baldinelli F, Broglia A, Dhollander S, Beltrán-Beck B, Kohnle L, Bicout D. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): Borna disease.. EFSA J 2017 Jul;15(7):e04951.
- van der Kolk JH. The equine species as Trojan horse for Borna Disease Virus-1?. Vet Q 2018 Dec;38(1):126-128.
- Kinnunen PM, Inkeroinen H, Ilander M, Kallio ER, Heikkilä HP, Koskela E, Mappes T, Palva A, Vaheri A, Kipar A, Vapalahti O. Intracerebral Borna disease virus infection of bank voles leading to peripheral spread and reverse transcription of viral RNA.. PLoS One 2011;6(8):e23622.
- Schindler AR, Vögtlin A, Hilbe M, Puorger M, Zlinszky K, Ackermann M, Ehrensperger F. Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts.. Mol Cell Probes 2007 Feb;21(1):47-55.
- Sauder C, Staeheli P. Rat model of borna disease virus transmission: epidemiological implications.. J Virol 2003 Dec;77(23):12886-90.
- Vahlenkamp TW, Konrath A, Weber M, Müller H. Persistence of Borna disease virus in naturally infected sheep.. J Virol 2002 Oct;76(19):9735-43.
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