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Journal of anatomy2016; 229(3); 450-458; doi: 10.1111/joa.12485

Distribution of proteins within different compartments of tendon varies according to tendon type.

Abstract: Although the predominant function of all tendons is to transfer force from muscle to bone and position the limbs, some tendons additionally function as energy stores, reducing the energetic cost of locomotion. To maximise energy storage and return, energy-storing tendons need to be more extensible and elastic than tendons with a purely positional function. These properties are conferred in part by a specialisation of a specific compartment of the tendon, the interfascicular matrix, which enables sliding and recoil between adjacent fascicles. However, the composition of the interfascicular matrix is poorly characterised and we therefore tested the hypothesis that the distribution of elastin and proteoglycans differs between energy-storing and positional tendons, and that protein distribution varies between the fascicular matrix and the interfascicular matrix, with localisation of elastin and lubricin to the interfascicular matrix. Protein distribution in the energy-storing equine superficial digital flexor tendon and positional common digital extensor tendon was assessed using histology and immunohistochemistry. The results support the hypothesis, demonstrating enrichment of lubricin in the interfascicular matrix in both tendon types, where it is likely to facilitate interfascicular sliding. Elastin was also localised to the interfascicular matrix, specifically in the energy-storing superficial digital flexor tendon, which may account for the greater elasticity of the interfascicular matrix in this tendon. A differential distribution of proteoglycans was identified between tendon types and regions, which may indicate a distinct role for each of these proteins in tendon. These data provide important advances into fully characterising structure-function relationships within tendon.
© 2016 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.
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Publication Date: 2016-04-25 PubMed ID: 27113131PubMed Central: PMC4974547DOI: 10.1111/joa.12485Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the difference in protein distributions within different parts of positional and energy-storing tendons, which contributes to their respective functionalities in body movement and energy storage.

Objective of the Research

  • The aim of this research was to closely examine the composition of a specific tendon compartment, known as the interfascicular matrix, which is responsible for providing elasticity to the tendons. The researchers hypothesised that the distribution of elastin and proteoglycans, two types of protein, differ between energy-storing tendons and positional tendons. They further theorised that the distribution of such proteins varies even in the different regions of a tendon, in the fascicular matrix and the interfascicular matrix.

Methodology of the Research

  • The research was conducted by examining the protein distribution in two types of tendons in horses – the energy-storing superficial digital flexor tendon and the positional common digital extensor tendon. The researchers used histology and immunohistochemistry to assess the protein distribution.

Findings of the Research

  • The study demonstrated that both the energy-storing superficial digital flexor tendon and the positional common digital extensor tendon had an enrichment of lubricin, a type of protein, within the interfascicular matrix. This protein likely facilitates the sliding action between fascicles.
  • The energy-storing tendons were found to contain elastin proteins uniquely in the interfascicular matrix, which could be the reason for the increased elasticity in these tendons.
  • Proteoglycans were distributed differently between the two types of tendons and even within their respective regions. This could suggest a distinct role for each of these proteins in tendon functionality.

Significance of the Research

  • The findings provide a significant contribution to our understanding of structure-function relationships in tendons. By establishing the links between protein distribution, and tendon type and functionality, the research can influence future studies or treatments related to tendons.

Cite This Article

APA
Thorpe CT, Karunaseelan KJ, Ng Chieng Hin J, Riley GP, Birch HL, Clegg PD, Screen HR. (2016). Distribution of proteins within different compartments of tendon varies according to tendon type. J Anat, 229(3), 450-458. https://doi.org/10.1111/joa.12485

Publication

Journal of anatomy
ISSN: 1469-7580
NlmUniqueID: 0137162
Country: England
Language: English
Volume: 229
Issue: 3
Pages: 450-458

Researcher Affiliations

Thorpe, Chavaunne T
  • Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, UK.
Karunaseelan, Kabelan J
  • Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, UK.
Ng Chieng Hin, Jade
  • Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, UK.
Riley, Graham P
  • School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, UK.
Birch, Helen L
  • Institute of Orthopaedics and Musculoskeletal Science, University College London, Royal National Orthopaedic Hospital, Stanmore, UK.
Clegg, Peter D
  • Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Neston, UK.
Screen, Hazel R C
  • Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, UK.

MeSH Terms

  • Animals
  • Elastin
  • Glycoproteins
  • Horses
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • Tendons / metabolism

Grant Funding

  • MR/K006312/1 / Medical Research Council

References

This article includes 42 references
  1. Batson EL, Paramour RJ, Smith TJ, Birch HL, Patterson-Kane JC, Goodship AE. Are the material properties and matrix composition of equine flexor and extensor tendons determined by their functions?. Equine Vet J 2003 May;35(3):314-8.
    pubmed: 12755437doi: 10.2746/042516403776148327google scholar: lookup
  2. Biewener AA. Muscle-tendon stresses and elastic energy storage during locomotion in the horse.. Comp Biochem Physiol B Biochem Mol Biol 1998 May;120(1):73-87.
    pubmed: 9787779doi: 10.1016/s0305-0491(98)00024-8google scholar: lookup
  3. Birch HL. Tendon matrix composition and turnover in relation to functional requirements.. Int J Exp Pathol 2007 Aug;88(4):241-8.
    pmc: PMC2517317pubmed: 17696905doi: 10.1111/j.1365-2613.2007.00552.xgoogle scholar: lookup
  4. Birch HL, Smith TJ, Draper ER. Collagen crosslink profile relates to tendon material properties.. Matrix Biol 2006 25, S74–S74.
  5. Birk DE, Nurminskaya MV, Zycband EI. Collagen fibrillogenesis in situ: fibril segments undergo post-depositional modifications resulting in linear and lateral growth during matrix development.. Dev Dyn 1995 Mar;202(3):229-43.
    pubmed: 7780173doi: 10.1002/aja.1002020303google scholar: lookup
  6. Dourte LM, Pathmanathan L, Jawad AF, Iozzo RV, Mienaltowski MJ, Birk DE, Soslowsky LJ. Influence of decorin on the mechanical, compositional, and structural properties of the mouse patellar tendon.. J Biomech Eng 2012 Mar;134(3):031005.
    pmc: PMC3705828pubmed: 22482685doi: 10.1115/1.4006200google scholar: lookup
  7. Ezura Y, Chakravarti S, Oldberg A, Chervoneva I, Birk DE. Differential expression of lumican and fibromodulin regulate collagen fibrillogenesis in developing mouse tendons.. J Cell Biol 2000 Nov 13;151(4):779-88.
    pmc: PMC2169450pubmed: 11076963doi: 10.1083/jcb.151.4.779google scholar: lookup
  8. Flannery CR, Hughes CE, Schumacher BL, Tudor D, Aydelotte MB, Kuettner KE, Caterson B. Articular cartilage superficial zone protein (SZP) is homologous to megakaryocyte stimulating factor precursor and Is a multifunctional proteoglycan with potential growth-promoting, cytoprotective, and lubricating properties in cartilage metabolism.. Biochem Biophys Res Commun 1999 Jan 27;254(3):535-41.
    pubmed: 9920774doi: 10.1006/bbrc.1998.0104google scholar: lookup
  9. Funakoshi T, Schmid T, Hsu HP, Spector M. Lubricin distribution in the goat infraspinatus tendon: a basis for interfascicular lubrication.. J Bone Joint Surg Am 2008 Apr;90(4):803-14.
    pubmed: 18381319doi: 10.2106/jbjs.g.00627google scholar: lookup
  10. Gosline J, Lillie M, Carrington E, Guerette P, Ortlepp C, Savage K. Elastic proteins: biological roles and mechanical properties.. Philos Trans R Soc Lond B Biol Sci 2002 Feb 28;357(1418):121-32.
    pmc: PMC1692928pubmed: 11911769doi: 10.1098/rstb.2001.1022google scholar: lookup
  11. Grant TM, Thompson MS, Urban J, Yu J. Elastic fibres are broadly distributed in tendon and highly localized around tenocytes.. J Anat 2013 Jun;222(6):573-9.
    pmc: PMC3666236pubmed: 23587025doi: 10.1111/joa.12048google scholar: lookup
  12. Innes JF, Clegg P. Comparative rheumatology: what can be learnt from naturally occurring musculoskeletal disorders in domestic animals?. Rheumatology (Oxford) 2010 Jun;49(6):1030-9.
    pubmed: 20176567doi: 10.1093/rheumatology/kep465google scholar: lookup
  13. Kalamajski S, Liu C, Tillgren V, Rubin K, Oldberg Å, Rai J, Weis M, Eyre DR. Increased C-telopeptide cross-linking of tendon type I collagen in fibromodulin-deficient mice.. J Biol Chem 2014 Jul 4;289(27):18873-9.
    pmc: PMC4081928pubmed: 24849606doi: 10.1074/jbc.m114.572941google scholar: lookup
  14. Kastelic J, Galeski A, Baer E. The multicomposite structure of tendon.. Connect Tissue Res 1978;6(1):11-23.
    pubmed: 149646doi: 10.3109/03008207809152283google scholar: lookup
  15. Kim B, Yoon JH, Zhang J, Eric Mueller PO, Halper J. Glycan profiling of a defect in decorin glycosylation in equine systemic proteoglycan accumulation, a potential model of progeroid form of Ehlers-Danlos syndrome.. Arch Biochem Biophys 2010 Sep 15;501(2):221-31.
    pubmed: 20599673doi: 10.1016/j.abb.2010.06.017google scholar: lookup
  16. Kohrs RT, Zhao C, Sun YL, Jay GD, Zhang L, Warman ML, An KN, Amadio PC. Tendon fascicle gliding in wild type, heterozygous, and lubricin knockout mice.. J Orthop Res 2011 Mar;29(3):384-9.
    pubmed: 20886657doi: 10.1002/jor.21247google scholar: lookup
  17. Lichtwark GA, Wilson AM. In vivo mechanical properties of the human Achilles tendon during one-legged hopping.. J Exp Biol 2005 Dec;208(Pt 24):4715-25.
    pubmed: 16326953doi: 10.1242/jeb.01950google scholar: lookup
  18. Lillie MA, Gosline JM. The viscoelastic basis for the tensile strength of elastin.. Int J Biol Macromol 2002 Apr 8;30(2):119-27.
    pubmed: 11911903doi: 10.1016/s0141-8130(02)00008-9google scholar: lookup
  19. Lord MS, Estrella RP, Chuang CY, Youssef P, Karlsson NG, Flannery CR, Whitelock JM. Not all lubricin isoforms are substituted with a glycosaminoglycan chain.. Connect Tissue Res 2012;53(2):132-41.
    pubmed: 21966936doi: 10.3109/03008207.2011.614364google scholar: lookup
  20. Lui PP, Maffulli N, Rolf C, Smith RK. What are the validated animal models for tendinopathy?. Scand J Med Sci Sports 2011 Feb;21(1):3-17.
    pubmed: 20673247doi: 10.1111/j.1600-0838.2010.01164.xgoogle scholar: lookup
  21. Maganaris CN, Paul JP. In vivo human tendon mechanical properties.. J Physiol 1999 Nov 15;521 Pt 1(Pt 1):307-13.
    pmc: PMC2269645pubmed: 10562354doi: 10.1111/j.1469-7793.1999.00307.xgoogle scholar: lookup
  22. Malliaras P, Cook J, Purdam C, Rio E. Patellar Tendinopathy: Clinical Diagnosis, Load Management, and Advice for Challenging Case Presentations.. J Orthop Sports Phys Ther 2015 Nov;45(11):887-98.
    pubmed: 26390269doi: 10.2519/jospt.2015.5987google scholar: lookup
  23. Rees SG, Flannery CR, Little CB, Hughes CE, Caterson B, Dent CM. Catabolism of aggrecan, decorin and biglycan in tendon.. Biochem J 2000 Aug 15;350 Pt 1(Pt 1):181-8.
    pmc: PMC1221240pubmed: 10926842
  24. Reuvers J, Thoreson AR, Zhao C, Zhang L, Jay GD, An KN, Warman ML, Amadio PC. The mechanical properties of tail tendon fascicles from lubricin knockout, wild type and heterozygous mice.. J Struct Biol 2011 Oct;176(1):41-5.
    pmc: PMC3910539pubmed: 21821131doi: 10.1016/j.jsb.2011.07.013google scholar: lookup
  25. Rigozzi S, Müller R, Stemmer A, Snedeker JG. Tendon glycosaminoglycan proteoglycan sidechains promote collagen fibril sliding-AFM observations at the nanoscale.. J Biomech 2013 Feb 22;46(4):813-8.
    pubmed: 23219277doi: 10.1016/j.jbiomech.2012.11.017google scholar: lookup
  26. Robinson PS, Huang TF, Kazam E, Iozzo RV, Birk DE, Soslowsky LJ. Influence of decorin and biglycan on mechanical properties of multiple tendons in knockout mice.. J Biomech Eng 2005 Feb;127(1):181-5.
    pubmed: 15868800doi: 10.1115/1.1835363google scholar: lookup
  27. Roughley PJ, White RJ, Cs-Szabó G, Mort JS. Changes with age in the structure of fibromodulin in human articular cartilage.. Osteoarthritis Cartilage 1996 Sep;4(3):153-61.
    pubmed: 8895216doi: 10.1016/s1063-4584(96)80011-2google scholar: lookup
  28. Schumacher BL, Hughes CE, Kuettner KE, Caterson B, Aydelotte MB. Immunodetection and partial cDNA sequence of the proteoglycan, superficial zone protein, synthesized by cells lining synovial joints.. J Orthop Res 1999 Jan;17(1):110-20.
    pubmed: 10073655doi: 10.1002/jor.1100170117google scholar: lookup
  29. Shine KM, Spector M. The presence and distribution of lubricin in the caprine intervertebral disc.. J Orthop Res 2008 Oct;26(10):1398-406.
    pubmed: 18464265doi: 10.1002/jor.20614google scholar: lookup
  30. Smith KD, Vaughan-Thomas A, Spiller DG, Innes JF, Clegg PD, Comerford EJ. The organisation of elastin and fibrillins 1 and 2 in the cruciate ligament complex.. J Anat 2011 Jun;218(6):600-7.
    pmc: PMC3125893pubmed: 21466551doi: 10.1111/j.1469-7580.2011.01374.xgoogle scholar: lookup
  31. Sun YL, Wei Z, Zhao C, Jay GD, Schmid TM, Amadio PC, An KN. Lubricin in human achilles tendon: The evidence of intratendinous sliding motion and shear force in achilles tendon.. J Orthop Res 2015 Jun;33(6):932-7.
    pubmed: 25864860doi: 10.1002/jor.22897google scholar: lookup
  32. Taguchi M, Sun YL, Zhao C, Zobitz ME, Cha CJ, Jay GD, An KN, Amadio PC. Lubricin surface modification improves tendon gliding after tendon repair in a canine model in vitro.. J Orthop Res 2009 Feb;27(2):257-63.
    pmc: PMC3329928pubmed: 18683890doi: 10.1002/jor.20731google scholar: lookup
  33. Thorpe CT, Streeter I, Pinchbeck GL, Goodship AE, Clegg PD, Birch HL. Aspartic acid racemization and collagen degradation markers reveal an accumulation of damage in tendon collagen that is enhanced with aging.. J Biol Chem 2010 May 21;285(21):15674-81.
    pmc: PMC2871433pubmed: 20308077doi: 10.1074/jbc.m109.077503google scholar: lookup
  34. Thorpe CT, Udeze CP, Birch HL, Clegg PD, Screen HR. Specialization of tendon mechanical properties results from interfascicular differences.. J R Soc Interface 2012 Nov 7;9(76):3108-17.
    pmc: PMC3479922pubmed: 22764132doi: 10.1098/rsif.2012.0362google scholar: lookup
  35. Thorpe CT, Birch HL, Clegg PD, Screen HR. The role of the non-collagenous matrix in tendon function.. Int J Exp Pathol 2013 Aug;94(4):248-59.
    pmc: PMC3721456pubmed: 23718692doi: 10.1111/iep.12027google scholar: lookup
  36. Thorpe CT, Klemt C, Riley GP, Birch HL, Clegg PD, Screen HR. Helical sub-structures in energy-storing tendons provide a possible mechanism for efficient energy storage and return.. Acta Biomater 2013 Aug;9(8):7948-56.
    pubmed: 23669621doi: 10.1016/j.actbio.2013.05.004google scholar: lookup
  37. Thorpe CT, Godinho MSC, Riley GP, Birch HL, Clegg PD, Screen HRC. The interfascicular matrix enables fascicle sliding and recovery in tendon, and behaves more elastically in energy storing tendons.. J Mech Behav Biomed Mater 2015 Dec;52:85-94.
    pmc: PMC4655227pubmed: 25958330doi: 10.1016/j.jmbbm.2015.04.009google scholar: lookup
  38. Thorpe CT, Chaudhry S, Lei II, Varone A, Riley GP, Birch HL, Clegg PD, Screen HR. Tendon overload results in alterations in cell shape and increased markers of inflammation and matrix degradation.. Scand J Med Sci Sports 2015 Aug;25(4):e381-91.
    pubmed: 25639911doi: 10.1111/sms.12333google scholar: lookup
  39. Thorpe CT, Peffers MJ, Simpson D, Halliwell E, Screen HR, Clegg PD. Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition.. Sci Rep 2016 Feb 4;6:20455.
    pmc: PMC4740843pubmed: 26842662doi: 10.1038/srep20455google scholar: lookup
  40. Viera AJ, Garrett JM. Understanding interobserver agreement: the kappa statistic.. Fam Med 2005 May;37(5):360-3.
    pubmed: 15883903
  41. Yoon JH, Halper J. Tendon proteoglycans: biochemistry and function.. J Musculoskelet Neuronal Interact 2005 Mar;5(1):22-34.
    pubmed: 15788868
  42. Zhang G, Ezura Y, Chervoneva I, Robinson PS, Beason DP, Carine ET, Soslowsky LJ, Iozzo RV, Birk DE. Decorin regulates assembly of collagen fibrils and acquisition of biomechanical properties during tendon development.. J Cell Biochem 2006 Aug 15;98(6):1436-49.
    pubmed: 16518859doi: 10.1002/jcb.20776google scholar: lookup

Citations

This article has been cited 32 times.
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