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Zygote (Cambridge, England)2021; 29(4); 325-330; doi: 10.1017/S0967199420000751

DNA fragmentation and morphometric studies in sperm of stallions supplemented with maca (Lepidium meyenii).

Abstract: The reproductive performances of livestock play an essential role in the economic management of the farm. The improvement of semen quantity and quality through the use of food supplements that lack substances which are forbidden in animal feeding, or that may have detrimental effects, is an important goal. Maca (Lepidium meyenii) is a plant that has been used for centuries in the Andes for nutrition and fertility enhancement in humans and animals. The aim of this study was to evaluate the effects of food supplementation of stallions with maca during the breeding season on spermatozoa parameters such as DNA fragmentation and shape, which are two predictive indexes of spermatozoa functionality. For this purpose, ejaculate volume, semen gel-free volume, sperm concentration and motility, total sperm count, sperm DNA fragmentation and sperm head parameters (length, width, perimeter, area, shape factor, roughness) were measured in four stallions. Maca food supplementation in stallions during breeding reduced the percentage of spermatozoa with fragmented DNA, increased significantly sperm concentration and exerted an elongation of the spermatozoa head, a condition that is believed to improve spermatozoa functionality, suggesting that food supplementation of maca could be useful in horse breeding during the breeding season.
Publication Date: 2021-02-10 PubMed ID: 33563356DOI: 10.1017/S0967199420000751Google Scholar: Lookup
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  • Journal Article

Summary

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The study explores the impact of maca (a plant native to the Andes, used for nutritional and fertility enhancement) supplementation in stallion’s diet on the quality and quantity of their sperm. The findings suggest that supplementing maca during the breeding season plays a role in reducing sperm DNA fragmentation, improving sperm concentration and changing sperm head shape, potentially improving overall reproduction performance in horse breeding.

Explaining the Research

The research paper revolves around the importance of optimizing reproductive performance in livestock, particularly horses, considering its influence on farm economics.

  • The researchers are interested in utilizing food supplements to enhance semen quality and quantity in stallions. The primary supplement under investigation in this case is maca (Lepidium meyenii), a plant already known to boost fertility in humans and animals.
  • The goal is to use maca supplementation in the feeding of stallions during breeding seasons, with the hope of causing positive adjustments in sperm parameters. It focuses specifically on characteristics like DNA fragmentation and sperm head shape, which are believed to be indicative of sperm functionality.

Methodology of the Study

  • This study involved monitoring four stallions that were offered maca-supplemented feed during the breeding season.
  • Various aspects of their semen were measured, including ejaculate volume, semen gel-free volume, sperm concentration and motility, total sperm count, and the extent of sperm DNA fragmentation. Additional sperm head parameters such as length, width, perimeter, area, shape, and roughness factor were also recorded.

Key Findings of the Study

The conclusion drawn from this study lies in the potential benefits of maca supplementation in stallions.

  • The feeding regimen appeared to decrease the percentage of sperm with fragmented DNA, signifying enhanced genetic integrity of the sperm.
  • Also, there was an increase in sperm concentration, which indicates an overall improvement in the fertility condition of the stallions.
  • Interestingly, researchers noted an elongation of the sperm heads, an alteration that could positively contribute to sperm functionality and therefore, the reproduction rate.

All these outcomes point towards the value of maca supplementation for stallions during breeding season as a potential strategy to secure better reproductive results within horse breeding practices.

Cite This Article

APA
D'Anza E, Albarella S, Galdiero G, Tafuri S, Del Prete C, Cocchia N, Ciani F, Mastellone V, Pasolini MP, Carotenuto D, Selvaggi M, Ciotola F, Peretti V. (2021). DNA fragmentation and morphometric studies in sperm of stallions supplemented with maca (Lepidium meyenii). Zygote, 29(4), 325-330. https://doi.org/10.1017/S0967199420000751

Publication

ISSN: 1469-8730
NlmUniqueID: 9309124
Country: England
Language: English
Volume: 29
Issue: 4
Pages: 325-330

Researcher Affiliations

D'Anza, Emanuele
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Albarella, Sara
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Galdiero, Giacomo
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Tafuri, Simona
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Del Prete, Chiara
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Cocchia, Natascia
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Ciani, Francesca
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Mastellone, Vincenzo
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Pasolini, Maria Pia
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Carotenuto, Domenico
  • UNMSM, Universidad Nacional Mayor San Marcos, San Borja, Lima, Peru.
Selvaggi, Maria
  • Department of Agricultural and Environmental Science, University of Bari Aldo Moro, Bari, Italy.
Ciotola, Francesca
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Peretti, Vincenzo
  • Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.

MeSH Terms

  • Animals
  • DNA Fragmentation
  • Dietary Supplements
  • Horses
  • Lepidium
  • Male
  • Plant Breeding
  • Spermatozoa

Citations

This article has been cited 3 times.
  1. Lee HW, Lee MS, Qu F, Lee JW, Kim E. Maca (Lepidium meyenii Walp.) on semen quality parameters: A systematic review and meta-analysis.. Front Pharmacol 2022;13:934740.
    doi: 10.3389/fphar.2022.934740pubmed: 36110519google scholar: lookup
  2. Del Prete C, Calabria A, Longobardi V, Palumbo V, Merlo B, Iacono E, Tafuri S, Carotenuto D, Ciani F, Damiano S, Ciarcia R, Cocchia N. Effect of Aqueous Extract of Maca Addition to an Extender for Chilled Canine Semen.. Animals (Basel) 2022 Jun 26;12(13).
    doi: 10.3390/ani12131638pubmed: 35804537google scholar: lookup
  3. Galdiero G, D'Anza E, de Angelis C, Albarella S, Peretti V, Pivonello R, Ciotola F. Sperm Global DNA Methylation (SGDM) in Semen of Healthy Dogs.. Vet Sci 2021 Mar 17;8(3).
    doi: 10.3390/vetsci8030050pubmed: 33802963google scholar: lookup