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Home / Equine Research Bank / Vet Sci / Does Double Centrifugation Lead to Premature Platelet Aggreg...
Veterinary sciences2019; 6(3); doi: 10.3390/vetsci6030068

Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma?

Abstract: Blood-derived autologous products are frequently used in both human and equine medicine to treat musculoskeletal disorders. These products, especially the platelet-rich plasma (PRP), may contain high concentrations of growth factors (GFs), and thus improve healing in several tissues. Nevertheless, the procedures for preparation of PRP are currently non-standardized. Several protocols, which are based on distinct centrifugation patterns (rotation speed and time), result in PRPs with different characteristics, concerning platelet and GFs concentrations, as well as platelet activation. The aim of the present study was to compare two different protocols for PRP preparation: protocol (A) that is based on a single-centrifugation step; protocol (B), which included two sequential centrifugation steps (double-centrifugation). The results here reported show that the double-centrifugation protocol resulted in higher platelet concentration, while leukocytes were not concentrated by this procedure. Although platelet activation and aggregation were increased in this protocol in comparison to the single-centrifugation one, the TGF-β1 concentration was also higher. Pearson's correlation coefficients gave a significant, positive correlation between the platelet counts and TGF-β1 concentration. In conclusion, although the double-centrifugation protocol caused premature platelet aggregation, it seems to be an effective method for preparation of PRP with high platelet and TGF-β1 concentrations.
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Publication Date: 2019-08-21 PubMed ID: 31438534PubMed Central: PMC6789863DOI: 10.3390/vetsci6030068Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the effectiveness of two different preparation methods for platelet-rich plasma (PRP), a treatment used in human and equine medicine for musculoskeletal disorders. It indicates that the double-centrifugation protocol, although leading to premature platelet aggregation, resulted in a higher concentration of platelets and TGF-β1 compared to the single-centrifugation method.

Introduction and Aim

  • The researchers aimed to compare two different protocols for preparing PRP: a single-centrifugation step (protocol A) and a double-centrifugation step (protocol B).
  • PRP, frequently used in human and equine medicine to treat musculoskeletal disorders, is important for its high concentration of growth factors which improves tissue healing.
  • The researchers note a lack of standardization in PRP preparation procedures, with different centrifugation patterns leading to varying results in platelet activation and growth factor concentration.

Results and Analysis

  • The study findings indicated that protocol B, the double-centrifugation method, produced a higher platelet concentration than protocol A.
  • This protocol did not result in a concentration of leukocytes, white cells involved in protecting the body against infectious disease and foreign invaders.
  • Despite causing increased platelet activation and aggregation, this method also resulted in a higher TGF-β1 concentration, a type of growth factor.
  • The correlation analysis (Pearson’s correlation) showed a significant positive correlation between platelet counts and TGF-β1 concentration, suggesting that the more platelets, the higher the TGF-β1 concentration.

Conclusion

  • While acknowledging that the double-centrifugation method does influence premature platelet aggregation, the researchers concluded it as an effective method for creating PRP with high concentrations of platelets and TGF-β1.
  • This could have implications for the efficiency of PRP preparation and its effectiveness in treating patients, given the importance of platelets and growth factors in tissue healing.

Cite This Article

APA
Seidel SRT, Vendruscolo CP, Moreira JJ, Fülber J, Ottaiano TF, Oliva MLV, Michelacci YM, Baccarin RYA. (2019). Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma? Vet Sci, 6(3). https://doi.org/10.3390/vetsci6030068

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 6
Issue: 3

Researcher Affiliations

Seidel, Sarah R T
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil. sarahseidel@usp.br.
Vendruscolo, Cynthia P
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Moreira, Juliana J
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Fülber, Joice
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Ottaiano, Tatiana F
  • Departamento de Bioquímica, Escola Paulista de Medicina, UNIFESP, São Paulo 04023-062, Brazil.
Oliva, Maria L V
  • Departamento de Bioquímica, Escola Paulista de Medicina, UNIFESP, São Paulo 04023-062, Brazil.
Michelacci, Yara M
  • Departamento de Bioquímica, Escola Paulista de Medicina, UNIFESP, São Paulo 04023-062, Brazil.
Baccarin, Raquel Y A
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil. baccarin@usp.br.

Conflict of Interest Statement

The authors declare that have no conflict of interest.

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