Dopamine receptors in equine ovarian tissues.
Abstract: Dopamine (DA) agonist and antagonist treatments can affect ovarian reproductive events in the mare. To support our theory that DA produces these effects by acting directly on the ovary, we analyzed equine ovarian tissues for the presence of dopamine receptor-1 (D1r) and dopamine receptor-2 (D2r) mRNA by reverse transcription polymerase chain reaction (RT-PCR) and D1r and D2r proteins by Western blot and immunohistochemistry (IHC). RT-PCR was performed on RNA isolated from ovarian cortex, medulla, granulosa/theca or corpus luteum (CL) tissues and from pituitary (D2r control) and renal artery (D1r control). D1r and D2r specific primers were designed from partial DNA sequences known for the horse (D2r) or conserved sequences from other species (D1r). Western blot analyses were conducted on CL, cortex and granulosa/theca samples and IHC was performed on CL tissues using D1r or D2r specific antibodies. The incidence of positive D2r mRNA was high in CL and ovarian cortex, low in granulosa/theca, and not detectable in ovarian medulla. Dopamine D1r mRNA incidence was high (50%) only in CL tissues. D1r and D2r antibody staining was positive for each tissue type analyzed by Western blot procedures. All CL tissues prepared by IHC showed positive staining for D1r and D2r proteins. Both DA receptor proteins appeared uniformly distributed throughout the CL tissue. These results indicate that equine ovarian tissues do possess D1r and D2r, and suggests that DA can act directly on ovarian tissues through its interaction with DA receptors.
Publication Date: 2005-04-14 PubMed ID: 15826775DOI: 10.1016/j.domaniend.2005.02.001Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigates the presence of dopamine receptors D1r and D2r in different parts of a horse’s ovary, and suggests that dopamine (DA) functions directly on these tissues by interacting with these receptors.
Methodology
- The researchers conducted their study on RNA isolated from various parts of the horse’s ovarian tissues, such as the cortex, the medulla, the granulosa cells and theca cells, as well as the corpus luteum (CL) tissues. RNA samples from pituitary and renal artery served as control groups for D2r and D1r, respectively.
- They designed D1r and D2r specific primers based on known partial DNA sequences from the horse or conserved sequences from other species.
- They employed reverse transcription polymerase chain reaction (RT-PCR), a method to convert an RNA sample into complementary DNA (cDNA) and amplify it, to detect the presence of D1r and D2r mRNA in these tissues.
- Moreover, they used Western blot, a technique to detect specific proteins in a sample, and immunohistochemistry (IHC), a method to visualize the location of proteins in tissue, to ensure the presence of D1r and D2r proteins.
Findings
- RT-PCR revealed a high incidence of D2r mRNA in the corpus luteum (CL) and ovarian cortex, low incidence in granulosa/thea cells, and no detectability in the ovarian medulla.
- The presence of D1r mRNA was high only in CL tissues.
- Western blot analysis and immunohistochemistry showed positive results for D1r and D2r protein in all distinctions analyzed.
- Both D1r and D2r proteins appeared to be uniformly dispersed across the CL tissue.
Conclusions
- The researchers concluded that the horse’s ovarian tissues carry dopamine receptors D1r and D2r.
- This discovery suggests that dopamine might exert its roles in reproductive events by interacting directly with these receptors in the ovary.
Cite This Article
APA
King SS, Campbell AG, Dille EA, Roser JF, Murphy LL, Jones KL.
(2005).
Dopamine receptors in equine ovarian tissues.
Domest Anim Endocrinol, 28(4), 405-415.
https://doi.org/10.1016/j.domaniend.2005.02.001 Publication
Researcher Affiliations
- Department of Animal Science, Food and Nutrition, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL 62901, USA.
MeSH Terms
- Animals
- Blotting, Western / veterinary
- Corpus Luteum / metabolism
- Female
- Gene Expression
- Granulosa Cells / metabolism
- Horses / metabolism
- Immunohistochemistry / veterinary
- RNA, Messenger / biosynthesis
- RNA, Messenger / genetics
- Receptors, Dopamine D1 / biosynthesis
- Receptors, Dopamine D1 / genetics
- Receptors, Dopamine D2 / biosynthesis
- Receptors, Dopamine D2 / genetics
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Theca Cells / metabolism
Citations
This article has been cited 5 times.- Zerani M, Polisca A, Boiti C, Maranesi M. Current Knowledge on the Multifactorial Regulation of Corpora Lutea Lifespan: The Rabbit Model. Animals (Basel) 2021 Jan 25;11(2).
- Kunii H, Nambo Y, Okano A, Matsui A, Ishimaru M, Asai Y, Sato F, Fujii K, Nagaoka K, Watanabe G, Taya K. Effects of an extended photoperiod on gonadal function and condition of hair coats in Thoroughbred colts and fillies. J Equine Sci 2015;26(2):57-66.
- Venegas-Meneses B, Padilla JF, Juárez CE, Morán JL, Morán C, Rosas-Murrieta NH, Handal A, Domínguez R. Effects of ovarian dopaminergic receptors on ovulation. Endocrine 2015 Dec;50(3):783-96.
- Parillo F, Maranesi M, Mignini F, Marinelli L, Di Stefano A, Boiti C, Zerani M. Evidence for a dopamine intrinsic direct role in the regulation of the ovary reproductive function: in vitro study on rabbit corpora lutea. PLoS One 2014;9(8):e104797.
- Rey-Ares V, Lazarov N, Berg D, Berg U, Kunz L, Mayerhofer A. Dopamine receptor repertoire of human granulosa cells. Reprod Biol Endocrinol 2007 Oct 25;5:40.
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