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Home / Equine Research Bank / J Orthop Res / Dual-contrast computed tomography enables detection of equin...
Journal of orthopaedic research : official publication of the Orthopaedic Research Society2021; 40(3); 703-711; doi: 10.1002/jor.25066

Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro.

Abstract: To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning. Posttraumatic changes include proteoglycan (PG) loss and elevated water content. Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes. Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture. Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies. Control samples were collected from three nonoperated ponies. The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points. As a reference, equilibrium moduli, dynamic moduli, and PG content were measured. Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min. Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content. QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
© 2021 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.
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Publication Date: 2021-05-12 PubMed ID: 33982283DOI: 10.1002/jor.25066Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper investigates the use of dual-energy computed tomography (QDECT) to detect posttraumatic osteoarthritis in horses. The study posits that QDECT can provide quantitative information related to proteoglycan loss and water content in the tissue surrounding cartilage injuries, thereby assisting in the diagnosis and treatment planning of the condition.

Objectives of the Study

The researchers of the study aimed to:

  • Determine the potential of QDECT in evaluating tissue quality surrounding cartilage lesions in horses.
  • Test the hypothesis that QDECT can detect posttraumatic degeneration by providing quantitative information of proteoglycan (PG) and water contents using a mixture of cationic and nonionic contrast agents.

Methodology

  • Chondral defects, or cartilage damage, were surgically created in both stifles (joint in the hind leg) of seven Shetland ponies.
  • Control samples were collected from three nonoperated ponies.
  • The experimental samples (14 in total) and the control samples (6 in total) were immersed in a mixture of contrast agents.
  • The distributions of the contrast agents were determined at various diffusion time points.
  • The equilibrium moduli, dynamic moduli, and PG content were measured to serve as a reference.

Findings

  • There were significant differences in partitions between the experimental and control samples demonstrated with the cationic contrast agent at 30 minutes, 60 minutes and 20 hours, and with the nonionic agent at 60 minutes and 120 minutes.
  • There were significant correlations obtained between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content at 20 and 24 hours.

Conclusion

  • The researchers concluded that QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of proteoglycan content.
  • Therefore, this technique could potentially advance the diagnostics of the extent and severity of cartilage injuries and assist in preventing the progression of posttraumatic osteoarthritis.

Cite This Article

APA
Saukko AEA, Nykänen O, Sarin JK, Nissi MJ, Te Moller NCR, Weinans H, Mancini IAD, Visser J, Brommer H, van Weeren PR, Malda J, Grinstaff MW, Töyräs J. (2021). Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro. J Orthop Res, 40(3), 703-711. https://doi.org/10.1002/jor.25066

Publication

Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
NlmUniqueID: 8404726
Country: United States
Language: English
Volume: 40
Issue: 3
Pages: 703-711

Researcher Affiliations

Saukko, Annina E A
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
  • Department of Medical Physics, Turku University Hospital, Turku, Finland.
Nykänen, Olli
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
  • Research Unit of Medical Imaging Physics and Technology, University of Oulu, Oulu, Finland.
Sarin, Jaakko K
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
  • Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland.
Nissi, Mikko J
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
  • Research Unit of Medical Imaging Physics and Technology, University of Oulu, Oulu, Finland.
Te Moller, Nikae C R
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Weinans, Harrie
  • Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Delft, The Netherlands.
  • Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands.
Mancini, Irina A D
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Visser, Jetze
  • Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands.
Brommer, Harold
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
van Weeren, P Réné
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Malda, Jos
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
  • Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands.
Grinstaff, Mark W
  • Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, Boston, Massachusetts, USA.
Töyräs, Juha
  • Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
  • Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland.
  • School of Information Technology and Electrical Engineering, The University of Queensland, Brisbane, Australia.
  • Science Service Center, Kuopio University Hospital, Kuopio, Finland.

MeSH Terms

  • Animals
  • Cartilage, Articular / pathology
  • Cations
  • Contrast Media
  • Horses
  • Osteoarthritis / diagnostic imaging
  • Osteoarthritis / etiology
  • Osteoarthritis / pathology
  • Proteoglycans
  • Tomography, X-Ray Computed
  • Water

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