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Frontiers in physiology2023; 14; 1256217; doi: 10.3389/fphys.2023.1256217

Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses.

Abstract: Important changes in glucose transporter (GLUT) expression should be expected if the glucose influx plays a pivotal role in fuelling or connecting metabolic pathways that are upregulated in response to exercise. The aim was to assess GLUT4, 8, and 12 dynamics in response to training and acute exercise. Sixteen untrained Standardbred mares (3-4 year) performed an incremental SET at the start and end of 8 weeks harness training. M. pectoralis (PM) and M. vastus lateralis (VL) muscle biopsies were taken before and after each SET, allowing for comparing rest and acute samples in untrained (UT) and trained (T) condition using Western Blot for GLUT quantification and Image Pro v.10 for Blot analysis. Data were normalized against GAPDH. Basal GLUT-levels of PM VL were analysed with the Wilcoxon matched-pairs signed rank test. The effect of acute exercise or training was assessed using the Friedman test with a Dunn's. Basal GLUT4 and GLUT12 protein expression were significantly higher in the VL compared to the PM (P = 0.031 and P = 0.002). Training had no effect on basal GLUT4 expression, neither in the VL ( > 0.9999), nor the PM ( > 0.9999). However, acute exercise in trained condition significantly decreased GLUT4 expression in the VL ( = 0.0148). Neither training nor acute exercise significantly changed total GLUT8 protein expression. Training significantly decreased total GLUT12 protein expression in rest biopsies, only visible in the VL ( = 0.0359). This decrease was even more prominent in the VL after acute exercise in trained condition (P = 0.0025). The important changes seen in GLUT12 expression downregulation, both in response to training and acute exercise in the horse, the downregulation of GLUT4 expression after acute exercise in trained condition and the lack of differential shifts in GLUT8 expression in any of the studied conditions, questions the importance of glucose as substrate to fuel training and exercise in healthy horses. These findings encourage to further explore alternative fuels for their involvement in equine muscular energetics.
Copyright © 2023 Vidal Moreno de Vega, Lemmens, de Meeûs d’Argenteuil, Boshuizen, de Maré, Leybaert, Goethals, de Oliveira, Hosotani, Deforce, Van Nieuwerburgh, Devisscher and Delesalle.
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Publication Date: 2023-08-16 PubMed ID: 37654675PubMed Central: PMC10466803DOI: 10.3389/fphys.2023.1256217Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aims to understand the changes in expression of glucose transporters (GLUT 4, 8, 12) in horses’ muscles, due to both training and acute exercise, and questions the importance of glucose as a fuel source during horse training and exercise. Using Standardbred mares, the researchers found significant changes specifically in the expression of GLUT 4 and 12, leading to the suggestion of exploring alternative fuels for equine muscular energetics.

Study Design

  • The researches utilized 16 untrained Standardbred mares, aged between 3 to 4 years, subjecting them to an incremental Standardized Exercise Test (SET) at the start and end of an 8-week harness training regime.
  • Muscle biopsies of the M. pectoralis (PM) and M. vastus lateralis (VL) were taken before and after each SET, for comparison between resting and acute exercise samples in both untrained (UT) and trained (T) conditions.
  • Quantification of the GLUT expression was performed using Western Blot, analyzed with Image Pro v.10. Data normalization was done against GAPDH.
  • Statistical analyses involved the Wilcoxon matched-pairs signed rank test for basal GLUT levels, and the Friedman test with a Dunn’s for examining the effect of acute exercise or training.

Findings

  • In resting conditions, GLUT4 and GLUT12 protein expression was found to be significantly higher in the VL as compared to the PM.
  • Training was found to have no effect on the basal expression of GLUT4 in either the VL or PM muscles.
  • However, acute exercise in trained horses was found to decrease GLUT4 expression significantly in the VL muscle.
  • There were no significant changes in total GLUT8 protein expression due to either acute exercise or training.
  • Training led to a significant decrease in total GLUT12 protein expression in resting biopsies, more prominently visible in the VL muscles.
  • This decrease in GLUT12 expression was more pronounced in the VL muscles after acute exercise in trained conditions.

Implications

  • The observed changes in GLUT12 expression – both in response to training and acute exercise in horses, downregulation of GLUT4 expression post acute exercise in trained conditions, and no significant shifts in GLUT8 expression – call for questioning the importance of glucose as a substrate to fuel training and exercise in horses.
  • The findings of this study hint at the potential need to explore alternate fuels, beyond glucose, for their involvement in equine muscular energetics.

Cite This Article

APA
Vidal Moreno de Vega C, Lemmens D, de Meeûs d'Argenteuil C, Boshuizen B, de Maré L, Leybaert L, Goethals K, de Oliveira JE, Hosotani G, Deforce D, Van Nieuwerburgh F, Devisscher L, Delesalle C. (2023). Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses. Front Physiol, 14, 1256217. https://doi.org/10.3389/fphys.2023.1256217

Publication

Frontiers in physiology
ISSN: 1664-042X
NlmUniqueID: 101549006
Country: Switzerland
Language: English
Volume: 14
Pages: 1256217
PII: 1256217

Researcher Affiliations

Vidal Moreno de Vega, Carmen
  • Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Lemmens, Diete
  • Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
de Meeûs d'Argenteuil, Constance
  • Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Boshuizen, Berit
  • Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
  • Wolvega Equine Hospital, Oldeholtpade, Netherlands.
de Maré, Lorie
  • Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Leybaert, Luc
  • Department of Basic and Applied Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.
Goethals, Klara
  • Biometrics Research Center, Ghent University, Ghent, Belgium.
de Oliveira, Jean Eduardo
  • Cargill R&D Centre Europe, Vilvoorde, Belgium.
Hosotani, Guilherme
  • Cargill R&D Centre Europe, Vilvoorde, Belgium.
Deforce, Dieter
  • Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
Van Nieuwerburgh, Filip
  • Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
Devisscher, Lindsey
  • Gut-Liver Immunopharmacology Unit, Department of Basic and Applied Medical Sciences, Liver Research Center Ghent, Ghent University, Ghent, Belgium.
Delesalle, Cathérine
  • Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Conflict of Interest Statement

JO and GH were employed by the Cargill R&D Centre Europe. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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