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Home / Equine Research Bank / Saudi J Biol Sci / Edible bird’s nest supplementation in chilled and cryo...
Saudi journal of biological sciences2021; 29(3); 1577-1584; doi: 10.1016/j.sjbs.2021.11.009

Edible bird’s nest supplementation in chilled and cryopreserved Arabian stallion semen.

Abstract: Diluents and various biological products have been used in different animal species, with promising outcomes in post-thaw sperm quality. Nevertheless, only a few reports are available for the semen of Arabian horses. Edible bird's nest (EBN) - a product of the salivary secretions of swiftlet species is widely known to have both antioxidant and anti-inflammatory properties. Presently, there is no data available on the role of EBN supplemented in different extenders and its effect on semen quality in stallion semen. Two in vitro experiments were conducted to examine the effects of edible bird's nest (EBN) on the quality of chilled and post-thawed cryopreserved Arabian stallion spermatozoa. In experiment one, 10 ejaculates were collected, divided into two equal parts, diluted using EquiPlus® and INRA 96® and supplemented with 0 % (control), 0.12 %, 0.24 % EBN concentrations. The semen samples were stored at 5 ℃ and observed at 0, 24, and 48 h. Sperm kinetics variables (% total motility [TM] and progressive motility [PM], curvilinear velocity; VCL, straightness; VSL, average path velocity; VAP) were analyzed using computerized assisted sperm analysis. For chilled semen, there was no significant difference in any of the sperm quality parameters between control (0 %), 0.12 %, and 0.24 % EBN supplementation either in INRA96® or EquiPlus®. In experiment two, nine ejaculates were diluted and cryopreserved using EquiPlus Freeze® and INRA Freeze® containing 0 %, 2.4 %, and 4.8 % EBN, and evaluated after thawing. Sperm kinetics, DNA integrity and antioxidant capacity - Biological Anti-oxidant Potential (BAP) and Reactive Oxygen Metabolites (d-ROMs) test were evaluated. In chilled semen, there was no significant difference in any of the sperm quality parameters between control (0 %), 0.12 %, and 0.24 % EBN supplementation either in INRA96® or EquiPlus®. For frozen semen supplemented with 2.4 % and 4.8 % EBN had higher sperm motility parameters compared to control in INRA Freeze® and EquiPlus Freeze®, but the values were not statistically significant (P > 0.05). Also, EBN supplementation had no significant effects on the DNA integrity, biological antioxidant potential, and reactive oxygen metabolites. EBN supplementation had no significant effects on sperm quality and antioxidant status in chilled and frozen Arabian Stallion semen. Future studies might consider different methods of EBN preparation and concentrations to elucidate the potential biological impact of EBN in Arabian stallion semen.
© 2021 The Author(s).
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Publication Date: 2021-11-12 PubMed ID: 35280569PubMed Central: PMC8913401DOI: 10.1016/j.sjbs.2021.11.009Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the effects of supplementing chilled and frozen Arabian stallion semen with edible bird’s nest (EBN), a bioproduct known for its antioxidant properties. It found that EBN didn’t significantly improve the quality or antioxidant status of the semen.

Objective and Methodology

  • The researchers wanted to understand the effect of EBN, known for its antioxidants and anti-inflammatory properties, on the quality of chilled and post-thawed cryopreserved Arabian stallion semen.
  • Two experiments were carried out in vitro to measure the impact of EBN supplementation in two types of diluents – EquiPlus® and INRA 96® – on horse spermatozoa.
  • Three concentration levels of EBN (0 %, 0.12 %, 0.24 %) were used to treat the semen which were then stored at 5 ℃ and checked at 0, 24, and 48 hours.
  • Various semen quality parameters were assessed, such as total motility [TM], progressive motility [PM], curvilinear velocity [VCL], straightness [VSL] and average path velocity [VAP].

Experiment Findings

  • The first experiment, conducted for chilled semen, didn’t show any significant difference in any of the observed sperm quality parameters between the control and the EBN supplemented versions.
  • The second experiment involved frozen semen samples supplemented with 0 %, 2.4 % and 4.8 % EBN and was observed post-thawing. The sperm quality, DNA integrity and antioxidant capacity were evaluated.
  • For the frozen semen, the EBN supplement resulted insignificant improvement in sperm motility parameters, but the differences were statistically insignificant.
  • There was also no significant difference in the DNA integrity, biological antioxidant potential, and reactive oxygen metabolites between the control and the EBN supplemented versions of the semen.

Conclusion

  • The results of the experiments indicated that adding EBN had no significant impact on the quality and antioxidant status of both chilled and frozen Arabian stallion semen.
  • The researchers suggest carrying out future studies, where different preparation methods and concentration of EBN could be used, to further explore its potential biological impact on Arabian stallion semen.

Cite This Article

APA
Al-Khaldi K, Yimer N, Sadiq MB, Firdaus Jesse Bin Abdullah F, Salam Babji A, Al-Bulushi S. (2021). Edible bird’s nest supplementation in chilled and cryopreserved Arabian stallion semen. Saudi J Biol Sci, 29(3), 1577-1584. https://doi.org/10.1016/j.sjbs.2021.11.009

Publication

Saudi journal of biological sciences
ISSN: 1319-562X
NlmUniqueID: 101543796
Country: Saudi Arabia
Language: English
Volume: 29
Issue: 3
Pages: 1577-1584

Researcher Affiliations

Al-Khaldi, Khalid
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Breeding Branch, Veterinary Department, Royal Oman Police, Mounted Police Division, Muscat, Sultanate of Oman.
Yimer, Nurhusien
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Sadiq, Mohammed B
  • Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Firdaus Jesse Bin Abdullah, Faez
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Salam Babji, Abdul
  • School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Al-Bulushi, Samir
  • Laboratories and Animal Research Centre, Directorate General of Veterinary Services, Royal Court Affairs, Muscat, Sultanate of Oman.

Conflict of Interest Statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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