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Theriogenology2000; 54(1); 129-136; doi: 10.1016/S0093-691X(00)00331-9

Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage.

Abstract: The objective of this study was to determine if centrifugation and partial removal of seminal plasma would improve spermatozoal motility in semen from stallions whose whole ejaculates have poor tolerance to cooling and storage. Stallions were divided into two groups (n = 5/group) based on the ability of their extended semen to maintain spermatozoal motility after cooling and storage. Group 1 stallions ("good coolers") produced semen in which progressive spermatozoal motility after 24 h of cooling and storage was reduced by < or = 30% of progressive motility prior to storage. Group 2 stallions ("poor coolers") produced semen in which progressive spermatozoal motility after 24 h of cooling and storage was reduced by > or = 40% of progressive motility prior to storage. The sperm-rich portion of each ejaculate was divided into 4 aliquots. Two aliquots underwent standard processing for cooled transported semen and were examined after 24 and 48 h of cooling and storage in an Equitainer. The remaining two aliquots were diluted 1:1 with semen extender, then centrifuged at 400 x g for 12 min at room temperature. After centrifugation, approximately 90% of the seminal plasma was removed, and the sperm pellet was resuspended in extender to a final concentration of 25 to 50 x 10(6) sperm/mL. These aliquots were then packaged as for the non-centrifuged aliquots and examined after 24 and 48 h of storage. The spermatozoal motion characteristics in fresh semen and after 24 and 48 h of cooling and storage was determined via computer-assisted semen analysis. Centrifugation and partial removal of seminal plasma increased the percentage of progressively motile spermatozoa and limited the reduction in progressive spermatozoal motility of "poor cooling" stallions after 48 h of cooling and storage. Results of this study indicate that centrifugation and partial removal of seminal plasma is beneficial for stallions whose ejaculates have poor tolerance to cooling and storage with routine semen dilution and packaging techniques, especially if the semen is stored for > 24 h.
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Publication Date: 2000-09-16 PubMed ID: 10990354DOI: 10.1016/S0093-691X(00)00331-9Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates whether centrifugation and partial removal of seminal plasma would enhance sperm motility in stallion semen that does not cope well with cooling and storage. The results showed that these techniques increase the percentage of motile sperm and limit the reduction in sperm movement for stallions that typically have poor outcomes after cooling and storage.

Research Objectives and Methodology

  • The primary goal of the study was to ascertain if centrifugation and partial removal of seminal plasma could improve the motility of the sperm in the semen of stallions that generally exhibit poor tolerance to cooling and storage.
  • For the study, stallions were segregated into two groups of five, with classification depending on how their extended (processed and preserved) semen maintained sperm motility after cooling and extended storage.
  • “Good coolers” were those stallions whose semen, after 24 hours of cooling and storage, demonstrated a decrease in progressive sperm motility by 30% or less. “Poor coolers,” conversely, produced semen that registered a drop in progressive sperm motility by 40% or more after the same duration of cooling and storage.

Procedures and Analysis

  • The sperm-intensive portion of each ejaculate was split into four aliquots. Two of these underwent a standard processing method designed for cooled transported semen and were then examined after 24 and 48 hours of cooling and storage.
  • The remaining two aliquots were diluted and centrifuged at room temperature. After this process, nearly 90% of the seminal plasma was removed, and the remaining sperm was resuspended in an extender to achieve a specific concentration. Post this, these aliquots were packaged and assessed after 24 and 48 hours of storage.
  • With the aid of computer-assisted semen analysis, sperm motion characteristics were determined both in fresh semen and after the designated cooling and storage durations.

Findings and Conclusion

  • The study revealed that centrifugation and the partial removal of seminal plasma caused an increase in the percentage of progressively motile spermatozoa.
  • This treatment also minimized the decrease in progressive sperm motility in the semen of “poor cooling” stallions after 48 hours of cooling and storage.
  • Consequently, the research concluded that the process of centrifugation and partial removal of seminal plasma is beneficial, particularly for stallions whose ejaculates generally exhibit poor tolerance to cooling and storage.
  • This is especially applicable when such semen needs to be stored for more than 24 hours using typical semen dilution and packaging techniques.

Cite This Article

APA
Brinsko SP, Crockett EC, Squires EL. (2000). Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage. Theriogenology, 54(1), 129-136. https://doi.org/10.1016/S0093-691X(00)00331-9

Publication

Theriogenology
ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 54
Issue: 1
Pages: 129-136

Researcher Affiliations

Brinsko, S P
  • Department of Clinical Sciences, Colorado State University, Fort Collins 80523, USA.
Crockett, E C
    Squires, E L

      MeSH Terms

      • Animals
      • Centrifugation / veterinary
      • Cold Temperature
      • Horses / physiology
      • Male
      • Semen
      • Semen Preservation / methods
      • Semen Preservation / veterinary
      • Sperm Motility

      Citations

      This article has been cited 15 times.
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