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ISRN veterinary science2013; 2013; 280143; doi: 10.1155/2013/280143

Effect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm.

Abstract: Cholesterol and Equex-STM are frequently added to different commercial and experimental extenders improving postthawing sperm quality. Doses of 125-150 mM of cholesterol from pig liver and 0.5-0.7% of Equex-STM were evaluated in a standard eggyolk extender (Martin et al., 1979). Six ejaculates per stallion from six pure Spanish stallions (6-8 years old) were collected in Martin's extender (B) and different mixtures of 125 mM-0.5% (I), 125 mM-0.7% (II), 150 mM-0.5% (III), and 150 mM-0.7% (IV) were added to original Martin's extender. Samples were frozen in 0.5 mL straws (100 × 10(6) spermatozoa) and thawed (21 s., 37°C water bath). After thawing the following parameters were evaluated: viability (V), motility (computer assisted sperm analysis, CASA; % nonprogressive NP; % progressive MP), hipoosmotic swelling test (HOST), acrosome integrity (A), fluorescence test (FL), and resistance test (RT). Sperm quality was significantly affected by stallion (in the parameters V, VI, NP, MP, HOST, A, FL, and RT), extraction (VI, NP, MP, HOST, A, and FL), and the different combinations of Equex-STM-cholesterol (FL). We concluded that 0.5% of Equex-STM mixed with 125 mM of cholesterol has obtained better sperm quality results than those of original Martin's extender, showing a simple and economic improvement of this home-made practical seminal extender.
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Publication Date: 2013-12-12 PubMed ID: 24416597PubMed Central: PMC3874953DOI: 10.1155/2013/280143Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the impact of adding cholesterol and Equex-STM to a standard egg yolk extender on the quality of cryopreserved sperm from pure Spanish stallions. The study concluded that a combination of 0.5% Equex-STM with 125 mM of cholesterol resulted in better sperm quality than the original extender.

Research Conduct and Methodology

  • The study examined the usage of cholesterol and Equex-STM in both commercial and experimental extenders used to improve the quality of sperm post-thawing.
  • The experiment involved six pure Spanish stallions aged between 6 and 8 years with six ejaculates taken from each.
  • A standard egg yolk extender, known as Martin’s extender, was used as the base for the experiment.
  • Four different mixtures of cholesterol (125 mM or 150 mM) from pig liver and Equex-STM (0.5% or 0.7%) were applied to the Martin’s extender.
  • The samples were frozen in 0.5 mL straws, each containing 100 × 10(6) spermatozoa, and later thawed in a 37°C water bath for 21 seconds.

Data Analysis and Findings

  • Post-thawing, various parameters were evaluated to assess the quality of sperm, including viability, motility (analyzed by computer-assisted sperm analysis), acrosome integrity, and response to several tests – hipoosmotic swelling test, fluorescence test, and resistance test.
  • The data showed that the quality of sperm was significantly influenced by the stallion, extraction method, and different combinations of Equex-STM and cholesterol, particularly with regards to the fluorescence test.
  • The combination that delivered superior sperm quality in contrast to the original Martin’s extender was 0.5% of Equex-STM mixed with 125 mM of cholesterol.

Conclusion and Significance

  • The research concluded that adding a mixture of 0.5% Equex-STM and 125 mM of cholesterol to a standard egg yolk extender improves the quality of cryopreserved sperm.
  • This presents a simple and cost-effective way of enhancing a home-made semen extender, which could have broader applications in the field of animal breeding and reproductive technologies.

Cite This Article

APA
Gil L, Galindo-Cardiel I, Malo C, González N, Alvarez C. (2013). Effect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm. ISRN Vet Sci, 2013, 280143. https://doi.org/10.1155/2013/280143

Publication

ISRN veterinary science
ISSN: 2090-4452
NlmUniqueID: 101568322
Country: Egypt
Language: English
Volume: 2013
Pages: 280143
PII: 280143

Researcher Affiliations

Gil, Lidia
  • Reproduction and Obstetrics Area, Department of Animal Pathology, Faculty of Veterinary, University of Zaragoza, Spain.
Galindo-Cardiel, Iván
  • WorldPathol Ltd. Co., Zaragoza, Spain.
Malo, C
  • Reproduction and Obstetrics Area, Department of Animal Pathology, Faculty of Veterinary, University of Zaragoza, Spain.
González, N
  • Reproduction and Obstetrics Area, Department of Animal Pathology, Faculty of Veterinary, University of Zaragoza, Spain.
Alvarez, C
  • Equine Reproduction Center, Torre Abejar, Garrapinillos, Zaragoza, Spain.

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