FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Stem cells international2015; 2016; 9364974; doi: 10.1155/2016/9364974

Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis.

Abstract: Mesenchymal stem cells have been identified in the synovial fluid of several species. This study was conducted to characterize chondroprogenitor (CP) cells in equine synovial fluid (SF) and to determine the effect of fibroblast growth factor 2 (FGF-2) on SF-CP monolayer proliferation and subsequent chondrogenesis. We hypothesized that FGF-2 would stimulate SF-CP proliferation and postexpansion chondrogenesis. SF aspirates were collected from adult equine joints. Colony-forming unit (CFU) assays were performed during primary cultures. At first passage, SF-cells were seeded at low density, with or without FGF-2. Following monolayer expansion and serial immunophenotyping, cells were transferred to chondrogenic pellet cultures. Pellets were analyzed for chondrogenic mRNA expression and cartilage matrix secretion. There was a mean of 59.2 CFU/mL of SF. FGF-2 increased the number of population doublings during two monolayer passages and halved the population doubling times. FGF-2 did not alter the immunophenotype of SF-CPs during monolayer expansion, nor did FGF-2 compromise chondrogenesis. Hypertrophic phenotypic markers were not expressed in control or FGF-2 groups. FGF-2 did prevent the development of a "fibroblastic" cell layer around pellet periphery. FGF-2 significantly accelerates in vitro SF-CP expansion, the major hurdle to clinical application of this cell population, without detrimentally affecting subsequent chondrogenic capacity.
Get Full Text
Publication Date: 2015-12-29 PubMed ID: 26839571PubMed Central: PMC4709790DOI: 10.1155/2016/9364974Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the role of fibroblast growth factor 2 (FGF-2) in the development of chondroprogenitor (CP) cells in horse joint fluid. The study found that FGF-2 effectively stimulated the growth of these cells without altering their characteristics or affecting their ability to form cartilage.

Study Design and Methodology

  • The research was carried out by collecting synovial fluid, the clear fluid that lubricates joints, from adult horse joints.
  • This fluid was then cultivated in labs to form colony-forming unit (CFU) in primary cultures, to understand the behavior and growth rate of CP cells.
  • At the first passage, the fluid cells were seeded at a low density, with some of them subjected to the effects of FGF-2, and some left without.
  • After the cells had gone through expansion and serial immunophenotyping (process of defining cells), they were moved to form chondrogenic (cartilage-forming) pellet cultures.

Results of the Study

  • The study found that the Fibroblast growth factor 2 (FGF-2) positively influenced the growth rate of the synovial fluid chondroprogenitor (SF-CP) cells, resulting in an increased number of cell divisions.
  • The results also showed that FGF-2 halved the time it took for the cell population to double, signifying a significant acceleration in growth.
  • It was noted that FGF-2 did not alter the immune characteristics of SF-CP cells during growth nor did it detrimentally affect the cells’ ability to form into cartilage.
  • Furthermore, FGF-2 also prevented the development of a “fibroblastic” cell layer around the pellet periphery. This is significant as fibroblast cells are responsible for the production of extracellular matrix and collagen, key components of connective tissues.
  • Lastly, hypertrophic phenotypic markers, commonly associated with the enlarged growth of an organ, were not expressed in either the control or FGF-2 groups, further supporting the hypothesis.

Conclusions Drawn from the Research

  • The results of this study demonstrate the potential application of FGF-2 in stimulating the growth of CP cells, crucial for the repair of cartilage tissues.
  • The findings suggest that FGF-2 could significantly accelerate the in vitro expansion of these cells, which is currently one of the major obstacles standing in the way of using this cell population in clinical applications.

Cite This Article

APA
Bianchessi M, Chen Y, Durgam S, Pondenis H, Stewart M. (2015). Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis. Stem Cells Int, 2016, 9364974. https://doi.org/10.1155/2016/9364974

Publication

Stem cells international
ISSN: 1687-966X
NlmUniqueID: 101535822
Country: United States
Language: English
Volume: 2016
Pages: 9364974
PII: 9364974

Researcher Affiliations

Bianchessi, Marta
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USA.
Chen, Yuwen
  • QPS Taiwan, Center of Toxicology and Preclinical Sciences, No. 103, Lane 169, Kangning Street, Xizhi District, New Taipei City 221, Taiwan.
Durgam, Sushmitha
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USA.
Pondenis, Holly
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USA.
Stewart, Matthew
  • Department of Veterinary Clinical Medicine, University of Illinois, Urbana, IL 61802, USA.

References

This article includes 57 references
  1. Aigner T, McKenna L. Molecular pathology and pathobiology of osteoarthritic cartilage.. Cell Mol Life Sci 2002 Jan;59(1):5-18.
    doi: 10.1007/s00018-002-8400-3pubmed: 11846033google scholar: lookup
  2. Gardner OF, Archer CW, Alini M, Stoddart MJ. Chondrogenesis of mesenchymal stem cells for cartilage tissue engineering.. Histol Histopathol 2013 Jan;28(1):23-42.
    pubmed: 23233057doi: 10.14670/hh-28.23google scholar: lookup
  3. Mobasheri A, Kalamegam G, Musumeci G, Batt ME. Chondrocyte and mesenchymal stem cell-based therapies for cartilage repair in osteoarthritis and related orthopaedic conditions.. Maturitas 2014 Jul;78(3):188-98.
    doi: 10.1016/j.maturitas.2014.04.017pubmed: 24855933google scholar: lookup
  4. Makris EA, Gomoll AH, Malizos KN, Hu JC, Athanasiou KA. Repair and tissue engineering techniques for articular cartilage.. Nat Rev Rheumatol 2015 Jan;11(1):21-34.
    doi: 10.1038/nrrheum.2014.157pmc: PMC4629810pubmed: 25247412google scholar: lookup
  5. Kang SW, Yoo SP, Kim BS. Effect of chondrocyte passage number on histological aspects of tissue-engineered cartilage.. Biomed Mater Eng 2007;17(5):269-76.
    pubmed: 17851169
  6. Stewart MC, Saunders KM, Burton-Wurster N, Macleod JN. Phenotypic stability of articular chondrocytes in vitro: the effects of culture models, bone morphogenetic protein 2, and serum supplementation.. J Bone Miner Res 2000 Jan;15(1):166-74.
    doi: 10.1359/jbmr.2000.15.1.166pubmed: 10646126google scholar: lookup
  7. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow.. Tissue Eng 1998 Winter;4(4):415-28.
    doi: 10.1089/ten.1998.4.415pubmed: 9916173google scholar: lookup
  8. Yoo JU, Barthel TS, Nishimura K, Solchaga L, Caplan AI, Goldberg VM, Johnstone B. The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells.. J Bone Joint Surg Am 1998 Dec;80(12):1745-57.
    pubmed: 9875932doi: 10.2106/00004623-199812000-00004google scholar: lookup
  9. Al-Nbaheen M, Vishnubalaji R, Ali D, Bouslimi A, Al-Jassir F, Megges M, Prigione A, Adjaye J, Kassem M, Aldahmash A. Human stromal (mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential.. Stem Cell Rev Rep 2013 Feb;9(1):32-43.
    doi: 10.1007/s12015-012-9365-8pmc: PMC3563956pubmed: 22529014google scholar: lookup
  10. Sakaguchi Y, Sekiya I, Yagishita K, Muneta T. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source.. Arthritis Rheum 2005 Aug;52(8):2521-9.
    doi: 10.1002/art.21212pubmed: 16052568google scholar: lookup
  11. Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, Moore RM, Gimble JM. Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow.. Vet Surg 2008 Dec;37(8):713-24.
    doi: 10.1111/j.1532-950x.2008.00462.xpmc: PMC2746327pubmed: 19121166google scholar: lookup
  12. Wegmeyer H, Bröske AM, Leddin M, Kuentzer K, Nisslbeck AK, Hupfeld J, Wiechmann K, Kuhlen J, von Schwerin C, Stein C, Knothe S, Funk J, Huss R, Neubauer M. Mesenchymal stromal cell characteristics vary depending on their origin.. Stem Cells Dev 2013 Oct 1;22(19):2606-18.
    doi: 10.1089/scd.2013.0016pmc: PMC3780294pubmed: 23676112google scholar: lookup
  13. Wickham MQ, Erickson GR, Gimble JM, Vail TP, Guilak F. Multipotent stromal cells derived from the infrapatellar fat pad of the knee.. Clin Orthop Relat Res 2003 Jul;(412):196-212.
    pubmed: 12838072doi: 10.1097/01.blo.0000072467.53786.cagoogle scholar: lookup
  14. Yoshimura H, Muneta T, Nimura A, Yokoyama A, Koga H, Sekiya I. Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle.. Cell Tissue Res 2007 Mar;327(3):449-62.
    doi: 10.1007/s00441-006-0308-zpubmed: 17053900google scholar: lookup
  15. Barry F, Boynton RE, Liu B, Murphy JM. Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components.. Exp Cell Res 2001 Aug 15;268(2):189-200.
    doi: 10.1006/excr.2001.5278pubmed: 11478845google scholar: lookup
  16. De Ugarte DA, Morizono K, Elbarbary A, Alfonso Z, Zuk PA, Zhu M, Dragoo JL, Ashjian P, Thomas B, Benhaim P, Chen I, Fraser J, Hedrick MH. Comparison of multi-lineage cells from human adipose tissue and bone marrow.. Cells Tissues Organs 2003;174(3):101-9.
    doi: 10.1159/000071150pubmed: 12835573google scholar: lookup
  17. Pelttari K, Winter A, Steck E, Goetzke K, Hennig T, Ochs BG, Aigner T, Richter W. Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice.. Arthritis Rheum 2006 Oct;54(10):3254-66.
    doi: 10.1002/art.22136pubmed: 17009260google scholar: lookup
  18. Jones EA, English A, Henshaw K, Kinsey SE, Markham AF, Emery P, McGonagle D. Enumeration and phenotypic characterization of synovial fluid multipotential mesenchymal progenitor cells in inflammatory and degenerative arthritis.. Arthritis Rheum 2004 Mar;50(3):817-27.
    doi: 10.1002/art.20203pubmed: 15022324google scholar: lookup
  19. Jones EA, Crawford A, English A, Henshaw K, Mundy J, Corscadden D, Chapman T, Emery P, Hatton P, McGonagle D. Synovial fluid mesenchymal stem cells in health and early osteoarthritis: detection and functional evaluation at the single-cell level.. Arthritis Rheum 2008 Jun;58(6):1731-40.
    doi: 10.1002/art.23485pubmed: 18512779google scholar: lookup
  20. Kurose R, Ichinohe S, Tajima G, Horiuchi S, Kurose A, Sawai T, Shimamura T. Characterization of human synovial fluid cells of 26 patients with osteoarthritis knee for cartilage repair therapy.. Int J Rheum Dis 2010 Feb 1;13(1):68-74.
    doi: 10.1111/j.1756-185x.2009.01456.xpubmed: 20374387google scholar: lookup
  21. Morito T, Muneta T, Hara K, Ju YJ, Mochizuki T, Makino H, Umezawa A, Sekiya I. Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans.. Rheumatology (Oxford) 2008 Aug;47(8):1137-43.
    doi: 10.1093/rheumatology/ken114pubmed: 18390894google scholar: lookup
  22. Sekiya I, Ojima M, Suzuki S, Yamaga M, Horie M, Koga H, Tsuji K, Miyaguchi K, Ogishima S, Tanaka H, Muneta T. Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis.. J Orthop Res 2012 Jun;30(6):943-9.
    doi: 10.1002/jor.22029pubmed: 22147634google scholar: lookup
  23. Ando W, Kutcher JJ, Krawetz R, Sen A, Nakamura N, Frank CB, Hart DA. Clonal analysis of synovial fluid stem cells to characterize and identify stable mesenchymal stromal cell/mesenchymal progenitor cell phenotypes in a porcine model: a cell source with enhanced commitment to the chondrogenic lineage.. Cytotherapy 2014 Jun;16(6):776-88.
    doi: 10.1016/j.jcyt.2013.12.003pubmed: 24529553google scholar: lookup
  24. Jones BA, Pei M. Synovium-derived stem cells: a tissue-specific stem cell for cartilage engineering and regeneration.. Tissue Eng Part B Rev 2012 Aug;18(4):301-11.
    doi: 10.1089/ten.teb.2012.0002pubmed: 22429320google scholar: lookup
  25. Krawetz RJ, Wu YE, Martin L, Rattner JB, Matyas JR, Hart DA. Synovial fluid progenitors expressing CD90+ from normal but not osteoarthritic joints undergo chondrogenic differentiation without micro-mass culture.. PLoS One 2012;7(8):e43616.
    doi: 10.1371/journal.pone.0043616pmc: PMC3430696pubmed: 22952721google scholar: lookup
  26. Stewart M, Chen Y, Caporali E, Stewart A. Isolation and chondrogenic differentiation of cells isolated from synovial fluid. Regenerative Medicine 2009;4(6):S27–S28.
  27. Alegre-Aguarón E, Desportes P, García-Álvarez F, Castiella T, Larrad L, Martínez-Lorenzo MJ. Differences in surface marker expression and chondrogenic potential among various tissue-derived mesenchymal cells from elderly patients with osteoarthritis.. Cells Tissues Organs 2012;196(3):231-40.
    doi: 10.1159/000334400pubmed: 22947769google scholar: lookup
  28. Lee WJ, Hah YS, Ock SA, Lee JH, Jeon RH, Park JS, Lee SI, Rho NY, Rho GJ, Lee SL. Cell source-dependent in vivo immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs.. Exp Cell Res 2015 May 1;333(2):273-288.
    doi: 10.1016/j.yexcr.2015.03.015pubmed: 25819273google scholar: lookup
  29. Tang HC, Chen WC, Chiang CW, Chen LY, Chang YC, Chen CH. Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel.. Int J Mol Sci 2015 Aug 7;16(8):18507-21.
    doi: 10.3390/ijms160818507pmc: PMC4581257pubmed: 26262616google scholar: lookup
  30. Solchaga LA, Penick K, Porter JD, Goldberg VM, Caplan AI, Welter JF. FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells.. J Cell Physiol 2005 May;203(2):398-409.
    doi: 10.1002/jcp.20238pubmed: 15521064google scholar: lookup
  31. Hiraki Y, Shukunami C, Iyama K, Mizuta H. Differentiation of chondrogenic precursor cells during the regeneration of articular cartilage.. Osteoarthritis Cartilage 2001;9 Suppl A:S102-8.
    doi: 10.1016/S1063-4584(01)94436-Xpubmed: 11680673google scholar: lookup
  32. Stewart AA, Byron CR, Pondenis H, Stewart MC. Effect of fibroblast growth factor-2 on equine mesenchymal stem cell monolayer expansion and chondrogenesis.. Am J Vet Res 2007 Sep;68(9):941-5.
    doi: 10.2460/ajvr.68.9.941pubmed: 17764407google scholar: lookup
  33. De Schauwer C, van de Walle GR, Piepers S, Hoogewijs MK, Govaere JL, Meyer E, van Soom A. Successful isolation of equine mesenchymal stromal cells from cryopreserved umbilical cord blood-derived mononuclear cell fractions.. Equine Vet J 2013 Jul;45(4):518-22.
    doi: 10.1111/evj.12003pubmed: 23206252google scholar: lookup
  34. De Schauwer C, Piepers S, Van de Walle GR, Demeyere K, Hoogewijs MK, Govaere JL, Braeckmans K, Van Soom A, Meyer E. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.. Cytometry A 2012 Apr;81(4):312-23.
    doi: 10.1002/cyto.a.22026pubmed: 22411893google scholar: lookup
  35. Kim YJ, Sah RL, Doong JY, Grodzinsky AJ. Fluorometric assay of DNA in cartilage explants using Hoechst 33258.. Anal Biochem 1988 Oct;174(1):168-76.
    doi: 10.1016/0003-2697(88)90532-5pubmed: 2464289google scholar: lookup
  36. Farndale RW, Sayers CA, Barrett AJ. A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.. Connect Tissue Res 1982;9(4):247-8.
    doi: 10.3109/03008208209160269pubmed: 6215207google scholar: lookup
  37. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.. Methods 2001 Dec;25(4):402-8.
    doi: 10.1006/meth.2001.1262pubmed: 11846609google scholar: lookup
  38. Inada M, Yasui T, Nomura S, Miyake S, Deguchi K, Himeno M, Sato M, Yamagiwa H, Kimura T, Yasui N, Ochi T, Endo N, Kitamura Y, Kishimoto T, Komori T. Maturational disturbance of chondrocytes in Cbfa1-deficient mice.. Dev Dyn 1999 Apr;214(4):279-90.
    doi: 10.1002/(SICI)1097-0177(199904)214:4<279::AID-AJA1>3.0.CO;2-Wpubmed: 10213384google scholar: lookup
  39. Kim IS, Otto F, Zabel B, Mundlos S. Regulation of chondrocyte differentiation by Cbfa1.. Mech Dev 1999 Feb;80(2):159-70.
    doi: 10.1016/s0925-4773(98)00210-xpubmed: 10072783google scholar: lookup
  40. Enomoto H, Enomoto-Iwamoto M, Iwamoto M, Nomura S, Himeno M, Kitamura Y, Kishimoto T, Komori T. Cbfa1 is a positive regulatory factor in chondrocyte maturation.. J Biol Chem 2000 Mar 24;275(12):8695-702.
    doi: 10.1074/jbc.275.12.8695pubmed: 10722711google scholar: lookup
  41. Arnold MA, Kim Y, Czubryt MP, Phan D, McAnally J, Qi X, Shelton JM, Richardson JA, Bassel-Duby R, Olson EN. MEF2C transcription factor controls chondrocyte hypertrophy and bone development.. Dev Cell 2007 Mar;12(3):377-89.
    doi: 10.1016/j.devcel.2007.02.004pubmed: 17336904google scholar: lookup
  42. Solchaga LA, Penick K, Goldberg VM, Caplan AI, Welter JF. Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cells.. Tissue Eng Part A 2010 Mar;16(3):1009-19.
    doi: 10.1089/ten.tea.2009.0100pmc: PMC2862658pubmed: 19842915google scholar: lookup
  43. Handorf AM, Li WJ. Fibroblast growth factor-2 primes human mesenchymal stem cells for enhanced chondrogenesis.. PLoS One 2011;6(7):e22887.
    doi: 10.1371/journal.pone.0022887pmc: PMC3144950pubmed: 21818404google scholar: lookup
  44. Jakobsen RB, Østrup E, Zhang X, Mikkelsen TS, Brinchmann JE. Analysis of the effects of five factors relevant to in vitro chondrogenesis of human mesenchymal stem cells using factorial design and high throughput mRNA-profiling.. PLoS One 2014;9(5):e96615.
    doi: 10.1371/journal.pone.0096615pmc: PMC4015996pubmed: 24816923google scholar: lookup
  45. Correa D, Somoza RA, Lin P, Greenberg S, Rom E, Duesler L, Welter JF, Yayon A, Caplan AI. Sequential exposure to fibroblast growth factors (FGF) 2, 9 and 18 enhances hMSC chondrogenic differentiation.. Osteoarthritis Cartilage 2015 Mar;23(3):443-53.
    doi: 10.1016/j.joca.2014.11.013pmc: PMC4692467pubmed: 25464167google scholar: lookup
  46. Goetz R, Mohammadi M. Exploring mechanisms of FGF signalling through the lens of structural biology.. Nat Rev Mol Cell Biol 2013 Mar;14(3):166-80.
    doi: 10.1038/nrm3528pmc: PMC3695728pubmed: 23403721google scholar: lookup
  47. Gospodarowicz D, Vlodavsky I, Savion N. The extracellular matrix and the control of proliferation of vascular endothelial and vascular smooth muscle cells.. J Supramol Struct 1980;13(3):339-72.
    doi: 10.1002/jss.400130307pubmed: 6451772google scholar: lookup
  48. Bianchi G, Banfi A, Mastrogiacomo M, Notaro R, Luzzatto L, Cancedda R, Quarto R. Ex vivo enrichment of mesenchymal cell progenitors by fibroblast growth factor 2.. Exp Cell Res 2003 Jul 1;287(1):98-105.
    doi: 10.1016/S0014-4827(03)00138-1pubmed: 12799186google scholar: lookup
  49. Gharibi B, Hughes FJ. Effects of medium supplements on proliferation, differentiation potential, and in vitro expansion of mesenchymal stem cells.. Stem Cells Transl Med 2012 Nov;1(11):771-82.
    doi: 10.5966/sctm.2010-0031pmc: PMC3659663pubmed: 23197689google scholar: lookup
  50. Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E, Takagishi K, Kato Y. Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF.. Biochem Biophys Res Commun 2001 Oct 26;288(2):413-9.
    doi: 10.1006/bbrc.2001.5777pubmed: 11606058google scholar: lookup
  51. Kurth TB, Dell'accio F, Crouch V, Augello A, Sharpe PT, De Bari C. Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo.. Arthritis Rheum 2011 May;63(5):1289-300.
    doi: 10.1002/art.30234pubmed: 21538315google scholar: lookup
  52. Stewart AA, Byron CR, Pondenis HC, Stewart MC. Effect of dexamethasone supplementation on chondrogenesis of equine mesenchymal stem cells.. Am J Vet Res 2008 Aug;69(8):1013-21.
    doi: 10.2460/ajvr.69.8.1013pubmed: 18672964google scholar: lookup
  53. Fujioka R, Aoyama T, Takakuwa T. The layered structure of the articular surface.. Osteoarthritis Cartilage 2013 Aug;21(8):1092-8.
    doi: 10.1016/j.joca.2013.04.021pubmed: 23680879google scholar: lookup
  54. Koelling S, Kruegel J, Irmer M, Path JR, Sadowski B, Miro X, Miosge N. Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis.. Cell Stem Cell 2009 Apr 3;4(4):324-35.
    doi: 10.1016/j.stem.2009.01.015pubmed: 19341622google scholar: lookup
  55. Zhang S, Muneta T, Morito T, Mochizuki T, Sekiya I. Autologous synovial fluid enhances migration of mesenchymal stem cells from synovium of osteoarthritis patients in tissue culture system.. J Orthop Res 2008 Oct;26(10):1413-8.
    doi: 10.1002/jor.20659pubmed: 18418888google scholar: lookup
  56. Williams R, Khan IM, Richardson K, Nelson L, McCarthy HE, Analbelsi T, Singhrao SK, Dowthwaite GP, Jones RE, Baird DM, Lewis H, Roberts S, Shaw HM, Dudhia J, Fairclough J, Briggs T, Archer CW. Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage.. PLoS One 2010 Oct 14;5(10):e13246.
    doi: 10.1371/journal.pone.0013246pmc: PMC2954799pubmed: 20976230google scholar: lookup
  57. Harvanová D, Tóthová T, Sarišský M, Amrichová J, Rosocha J. Isolation and characterization of synovial mesenchymal stem cells.. Folia Biol (Praha) 2011;57(3):119-24.
    pubmed: 21888835

Citations

This article has been cited 8 times.
  1. Wong CC, Ou KL, Lin YH, Lin MF, Yang TL, Chen CH, Chan WP. Platelet-Rich Fibrin Facilitates One-Stage Cartilage Repair by Promoting Chondrocytes Viability, Migration, and Matrix Synthesis. Int J Mol Sci 2020 Jan 16;21(2).
    doi: 10.3390/ijms21020577pubmed: 31963217google scholar: lookup
  2. Zhou S, Chen S, Jiang Q, Pei M. Determinants of stem cell lineage differentiation toward chondrogenesis versus adipogenesis. Cell Mol Life Sci 2019 May;76(9):1653-1680.
    doi: 10.1007/s00018-019-03017-4pubmed: 30689010google scholar: lookup
  3. He R, Wang B, Cui M, Xiong Z, Lin H, Zhao L, Li Z, Wang Z, Peggrem S, Xia Z, Shao Z. Link Protein N-Terminal Peptide as a Potential Stimulating Factor for Stem Cell-Based Cartilage Regeneration. Stem Cells Int 2018;2018:3217895.
    doi: 10.1155/2018/3217895pubmed: 29531532google scholar: lookup
  4. Wong CC, Kuo TF, Yang TL, Tsuang YH, Lin MF, Chang CH, Lin YH, Chan WP. Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis. Int J Mol Sci 2017 Aug 7;18(8).
    doi: 10.3390/ijms18081722pubmed: 28783120google scholar: lookup
  5. Branly T, Bertoni L, Contentin R, Rakic R, Gomez-Leduc T, Desancé M, Hervieu M, Legendre F, Jacquet S, Audigié F, Denoix JM, Demoor M, Galéra P. Characterization and use of Equine Bone Marrow Mesenchymal Stem Cells in Equine Cartilage Engineering. Study of their Hyaline Cartilage Forming Potential when Cultured under Hypoxia within a Biomaterial in the Presence of BMP-2 and TGF-ß1. Stem Cell Rev Rep 2017 Oct;13(5):611-630.
    doi: 10.1007/s12015-017-9748-ypubmed: 28597211google scholar: lookup
  6. Herzog K, Nguyen-Edquilang J, Stewart M. Comparative In Vitro Osteogenic Capacities of Bone Marrow- and Periosteal-Derived Progenitor Cells. Biology (Basel) 2025 Oct 2;14(10).
    doi: 10.3390/biology14101354pubmed: 41154757google scholar: lookup
  7. King EM, Wilson JM, Hostnik ET, Bapodra P, Junge RE, Niehaus AJ, Durgam SS, Schreeg ME. Chronic osteoarthritis caused by Propionibacterium australiense infection in a captive sand gazelle. J Vet Diagn Invest 2024 Nov;36(6):816-822.
    doi: 10.1177/10406387241263329pubmed: 39101552google scholar: lookup
  8. Jia Z, Liang Y, Li X, Xu X, Xiong J, Wang D, Duan L. Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model. J Vis Exp 2018 Aug 10;(138).
    doi: 10.3791/57466pubmed: 30148486google scholar: lookup
Subscribe to our newsletter
Join 99,911 horse owners like you who receive our email updates on horse health and nutrition.