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Home / Equine Research Bank / Can Vet J / Effect of furosemide and furosemide-carbazochrome combinatio...
The Canadian veterinary journal = La revue veterinaire canadienne2009; 50(8); 821-827;

Effect of furosemide and furosemide-carbazochrome combination on exercise-induced pulmonary hemorrhage in Standardbred racehorses.

Abstract: The objective was to quantify the effect of furosemide and carbazochrome on exercise-induced pulmonary hemorrhage (EIPH) in Standardbred horses using red blood cell count and hemoglobin concentration in bronchoalveolar lavage (BAL) fluid. Six healthy Standardbred horses with prior evidence of EIPH performed a standardized treadmill test 4 h after administration of placebo, furosemide, or furosemide-carbazochrome combination. Red blood cell (RBC) counts and hemoglobin concentrations were determined on the BAL fluid. The RBC count in BAL ranges were (2903-26,025 cells/microL), (45-24,060 cells/microL), and (905-3045 cells/microL) for placebo, furosemide, and furosemide-carbazochrome, respectively. Hemoglobin concentration ranges were (0.03-0.59 mg/mL), (0.01-0.55 mg/mL), and (0.007-0.16 mg/mL) for placebo, furosemide, and furosemide-carbazochrome groups, respectively. No significant differences were detected among treatments. However, there was great variability among horses, suggesting that a larger sample size or better selection of horses was needed. L’objectif était de quantifier l’effet du furosémide et du carbazochrome sur l’hémorragie pulmonaire induite par l’exercice (HPIE) chez les chevaux Standardbred en utilisant la numération des globules rouges et la concentration d’hémoglobine dans le liquide de lavage bronchoalvéolaire (LBA). Six chevaux Standardbred en santé avec des signes antérieurs d’HPIE ont effectué un test normalisé sur tapis roulant 4 heures après l’administration du placebo, du furosémide ou de la combinaison furosémide–carbazochrome. Les numérations des globules rouges (GR) et les concentrations d’hémoglobine ont été déterminées à partir du liquide du LBA. Les intervalles de numération des GR dans le LBA était de [2903–26 025 cellules/μL], [445–24 060] et [905–3045] pour les groupes placebo, furosémide et furosémide–carbazochrome, respectivement. Les intervalles de concentration de l’hémoglobine étaient de [0,03–0,59 mg/mL], [0,01–0,55] et de [0,007–0,16] pour les groupes placebo, furosémide et furosémide — carbazochrome, respectivement. Aucune différence significative n’a été détectée parmi les traitements. Cependant, la variabilité entre les chevaux était très grande, suggérant qu’une taille d’échantillon plus importante ou une meilleure sélection des chevaux était requise. (Traduit par Isabelle Vallières)
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Publication Date: 2009-11-03 PubMed ID: 19881919PubMed Central: PMC2711466
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examined the impact of furosemide and a furosemide-carbazochrome combo on exercise-induced pulmonary hemorrhage in Standardbred racehorses, measured through red blood cell count and hemoglobin concentration. No significant difference was found among the treatments, yet substantial variability among horses indicated the need for a larger sample size or improved selection of horses.

Objective of the Study

  • The aim of the research was to assess the effect of two treatments – furosemide and a combination of furosemide and carbazochrome, on the condition of exercise-induced pulmonary hemorrhage (EIPH) in Standardbred racehorses.
  • To measure the effect, researchers used the red blood cell (RBC) count and hemoglobin concentration in bronchoalveolar lavage (BAL) fluid. This fluid is collected from the lungs of horses and analyzed to determine the severity of EIPH.

Methods and Trial Procedure

  • Six healthy Standardbred horses with previous evidence of EIPH were selected.
  • They underwent a standard treadmill test four hours after the administration of either a placebo, furosemide, or a combination of furosemide and carbazochrome.
  • RBC counts and hemoglobin concentrations were determined using the collected BAL fluid.

Results of the Study

  • Despite differing ranges recorded for both measures in placebo, furosemide, and furosemide-carbazochrome groups, no significant differences were found among treatments.
  • The variability was high among horses, which suggested a larger sample size or better selection of horses may produce more accurate results in further studies.

Implications of the Research

  • While this research didn’t conclusively endorse the use of furosemide or its combination with carbazochrome for managing EIPH in Standardbred racehorses, it shed light on the need for more comprehensive studies.
  • The significant variability among individual horses underlines that a larger sample size could help attain better statistical validity and reliability. Additionally, refining the selection criteria for horses could contribute to more valuable results.

Cite This Article

APA
Perez-Moreno CI, Couëtil LL, Pratt SM, Ochoa-Acuña HG, Raskin RE, Russell MA. (2009). Effect of furosemide and furosemide-carbazochrome combination on exercise-induced pulmonary hemorrhage in Standardbred racehorses. Can Vet J, 50(8), 821-827.

Publication

The Canadian veterinary journal = La revue veterinaire canadienne
ISSN: 0008-5286
NlmUniqueID: 0004653
Country: Canada
Language: English
Volume: 50
Issue: 8
Pages: 821-827

Researcher Affiliations

Perez-Moreno, Cecilia I
  • Animal Science, Purdue University, West Lafayette, Indiana 47906, USA.
Couëtil, Laurent L
    Pratt, Suzanne M
      Ochoa-Acuña, Hugo G
        Raskin, Rose E
          Russell, Mark A

            MeSH Terms

            • Adrenochrome / analogs & derivatives
            • Adrenochrome / therapeutic use
            • Animals
            • Bronchoalveolar Lavage Fluid / cytology
            • Cross-Over Studies
            • Diuretics / therapeutic use
            • Drug Therapy, Combination
            • Exercise Test / veterinary
            • Female
            • Furosemide / therapeutic use
            • Hemorrhage / drug therapy
            • Hemorrhage / veterinary
            • Horse Diseases / drug therapy
            • Horses
            • Lung Diseases / drug therapy
            • Lung Diseases / veterinary
            • Male
            • Physical Conditioning, Animal / adverse effects
            • Random Allocation
            • Treatment Outcome

            References

            This article includes 45 references
            1. Lapointe JM, Vrins A, McCarvill E. A survey of exercise-induced pulmonary haemorrhage in Q standardbred racehorses.. Equine Vet J 1994 Nov;26(6):482-5.
              pubmed: 7889923doi: 10.1111/j.2042-3306.1994.tb04054.xgoogle scholar: lookup
            2. Birks EK, Shuler KM, Soma LR, Martin BB, Marconato L, Del Piero F, Teleis DC, Schar D, Hessinger AE, Uboh CE. EIPH: postrace endoscopic evaluation of Standardbreds and Thoroughbreds.. Equine Vet J Suppl 2002 Sep;(34):375-8.
              pubmed: 12405719doi: 10.1111/j.2042-3306.2002.tb05451.xgoogle scholar: lookup
            3. Kindig CA, McDonough P, Fenton G, Poole DC, Erickson HH. Efficacy of nasal strip and furosemide in mitigating EIPH in Thoroughbred horses.. J Appl Physiol (1985) 2001 Sep;91(3):1396-400.
              pubmed: 11509541doi: 10.1152/jappl.2001.91.3.1396google scholar: lookup
            4. Hinchcliff KW, Jackson MA, Morley PS, Brown JA, Dredge AE, O'Callaghan PA, McCaffrey JP, Slocombe RE, Clarke AE. Association between exercise-induced pulmonary hemorrhage and performance in Thoroughbred racehorses.. J Am Vet Med Assoc 2005 Sep 1;227(5):768-74.
              pubmed: 16178399doi: 10.2460/javma.2005.227.768google scholar: lookup
            5. West JB, Mathieu-Costello O. Stress failure of pulmonary capillaries as a mechanism for exercise induced pulmonary haemorrhage in the horse.. Equine Vet J 1994 Nov;26(6):441-7.
              pubmed: 7889916doi: 10.1111/j.2042-3306.1994.tb04047.xgoogle scholar: lookup
            6. West JB, Mathieu-Costello O, Jones JH, Birks EK, Logemann RB, Pascoe JR, Tyler WS. Stress failure of pulmonary capillaries in racehorses with exercise-induced pulmonary hemorrhage.. J Appl Physiol (1985) 1993 Sep;75(3):1097-109.
              pubmed: 8226517doi: 10.1152/jappl.1993.75.3.1097google scholar: lookup
            7. McKeever KH, Hinchcliff KW, Kociba GJ, Reed SM, Muir WW 3rd. Changes in coagulation and fibrinolysis in horses during exercise.. Am J Vet Res 1990 Sep;51(9):1335-9.
              pubmed: 2396779
            8. Johnstone IB, Viel L, Crane S, Whiting T. Hemostatic studies in racing standardbred horses with exercise-induced pulmonary hemorrhage. Hemostatic parameters at rest and after moderate exercise.. Can J Vet Res 1991 Apr;55(2):101-6.
              pmc: PMC1263427pubmed: 1909208
            9. Smith JM, Meyers KM, Barbee DD, Schott H, Bayly WM. Plasma von Willebrand factor in thoroughbreds in response to high-intensity treadmill exercise.. Am J Vet Res 1997 Jan;58(1):71-6.
              pubmed: 8989500
            10. Weiss DJ, McClay CB, Smith CM, Rao GH, White JG. Platelet function in the racing thoroughbred: implication for exercise-induced pulmonary hemorrhage.. Vet Clin Pathol 1990;19(2):35-39.
              pubmed: 12684935doi: 10.1111/j.1939-165x.1990.tb00540.xgoogle scholar: lookup
            11. Lester G, Clark C, Rice B, Steible-Hartless C, Vetro-Widenhouse T. Effect of timing and route of administration of furosemide on pulmonary hemorrhage and pulmonary arterial pressure in exercising thoroughbred racehorses.. Am J Vet Res 1999 Jan;60(1):22-8.
              pubmed: 9918144
            12. Goetz TE, Manohar M, Magid JH. Repeated administration of frusemide does not offer an advantage over single dosing in attenuating exercise-induced pulmonary hypertension in thoroughbred horses.. Equine Vet J Suppl 1999 Jul;(30):539-45.
              pubmed: 10659314doi: 10.1111/j.2042-3306.1999.tb05280.xgoogle scholar: lookup
            13. Pascoe JR, McCabe AE, Franti CE, Arthur RM. Efficacy of furosemide in the treatment of exercise-induced pulmonary hemorrhage in Thoroughbred racehorses.. Am J Vet Res 1985 Sep;46(9):2000-3.
              pubmed: 4051304
            14. Speirs VC, van Veenendaal JC, Harrison IW, Smyth GB, Anderson GA, Wilson DV, Gilbo B. Pulmonary haemorrhage in standardbred horses after racing.. Aust Vet J 1982 Aug;59(2):38-40.
              pubmed: 6890804doi: 10.1111/j.1751-0813.1982.tb02712.xgoogle scholar: lookup
            15. Sendo T, Goromaru T, Aki K, Sakai N, Itoh Y, Oishi R. Carbazochrome attenuates pulmonary dysfunction induced by a radiographic contrast medium in rats.. Eur J Pharmacol 2002 Aug 23;450(2):203-8.
              pubmed: 12206859doi: 10.1016/s0014-2999(02)02120-9google scholar: lookup
            16. Sendo T, Itoh Y, Aki K, Oka M, Oishi R. Carbazochrome sodium sulfonate (AC-17) reverses endothelial barrier dysfunction through inhibition of phosphatidylinositol hydrolysis in cultured porcine endothelial cells.. Naunyn Schmiedebergs Arch Pharmacol 2003 Sep;368(3):175-80.
              pubmed: 12928765doi: 10.1007/s00210-003-0785-5google scholar: lookup
            17. Couëtil LL, Denicola DB. Blood gas, plasma lactate and bronchoalveolar lavage cytology analyses in racehorses with respiratory disease.. Equine Vet J Suppl 1999 Jul;(30):77-82.
              pubmed: 10659227doi: 10.1111/j.2042-3306.1999.tb05193.xgoogle scholar: lookup
            18. Sánchez A, Couëtil LL, Ward MP, Clark SP. Effect of airway disease on blood gas exchange in racehorses.. J Vet Intern Med 2005 Jan-Feb;19(1):87-92.
              pubmed: 15715053doi: 10.1892/0891-6640(2005)19<87:eoadob>2.0.co;2google scholar: lookup
            19. Couroucé-Malblanc A, Pronost S, Fortier G, Corde R, Rossignol F. Physiological measurements and upper and lower respiratory tract evaluation in French Standardbred Trotters during a standardised exercise test on the treadmill.. Equine Vet J Suppl 2002 Sep;(34):402-7.
              pubmed: 12405724doi: 10.1111/j.2042-3306.2002.tb05456.xgoogle scholar: lookup
            20. Hinchcliff KW, Jackson MA, Brown JA, Dredge AF, O'Callaghan PA, McCaffrey JP, Morley PS, Slocombe RE, Clarke AF. Tracheobronchoscopic assessment of exercise-induced pulmonary hemorrhage in horses.. Am J Vet Res 2005 Apr;66(4):596-8.
              pubmed: 15900938doi: 10.2460/ajvr.2005.66.596google scholar: lookup
            21. Gerber V, Lindberg A, Berney C, Robinson NE. Airway mucus in recurrent airway obstruction--short-term response to environmental challenge.. J Vet Intern Med 2004 Jan-Feb;18(1):92-7.
              pubmed: 14765737doi: 10.1892/0891-6640(2004)18<92:amirao>2.0.co;2google scholar: lookup
            22. Couroucé A. Field exercise testing for assessing fitness in French standardbred trotters.. Vet J 1999 Mar;157(2):112-22.
              pubmed: 10204407doi: 10.1053/tvjl.1998.0302google scholar: lookup
            23. Burrell MH. Endoscopic and virological observations on respiratory disease in a group of young Thoroughbred horses in training.. Equine Vet J 1985 Mar;17(2):99-103.
              pubmed: 2985380doi: 10.1111/j.2042-3306.1985.tb02059.xgoogle scholar: lookup
            24. Moore BR, Krakowka S, Robertson JT, Cummins JM. Cytologic evaluation of bronchoalveolar lavage fluid obtained from standardbred racehorses with inflammatory airway disease.. Am J Vet Res 1995 May;56(5):562-7.
              pubmed: 7661448
            25. Sweeney CR, Rossier Y, Ziemer EL, Lindborg SR. Effect of prior lavage on bronchoalveolar lavage fluid cell population of lavaged and unlavaged lung segments in horses.. Am J Vet Res 1994 Nov;55(11):1501-4.
              pubmed: 7879970
            26. Gerber V, Robinson NE, Luethi S, Marti E, Wampfler B, Straub R. Airway inflammation and mucus in two age groups of asymptomatic well-performing sport horses.. Equine Vet J 2003 Jul;35(5):491-5.
              pubmed: 12875328doi: 10.2746/042516403775600424google scholar: lookup
            27. Newton JR, Wood JL. Evidence of an association between inflammatory airway disease and EIPH in young Thoroughbreds during training.. Equine Vet J Suppl 2002 Sep;(34):417-24.
              pubmed: 12405727doi: 10.1111/j.2042-3306.2002.tb05459.xgoogle scholar: lookup
            28. McKane SA, Slocombe RF. Alveolar fibrosis and changes in equine lung morphometry in response to intrapulmonary blood.. Equine Vet J Suppl 2002 Sep;(34):451-8.
              pubmed: 12405733doi: 10.1111/j.2042-3306.2002.tb05465.xgoogle scholar: lookup
            29. McKane SA, Canfield PJ, Rose RJ. Equine bronchoalveolar lavage cytology: survey of thoroughbred racehorses in training.. Aust Vet J 1993 Nov;70(11):401-4.
              pubmed: 8280021doi: 10.1111/j.1751-0813.1993.tb06072.xgoogle scholar: lookup
            30. Meyer TS, Fedde MR, Gaughan EM, Langsetmo I, Erickson HH. Quantification of exercise-induced pulmonary haemorrhage with bronchoalveolar lavage.. Equine Vet J 1998 Jul;30(4):284-8.
              pubmed: 9705109doi: 10.1111/j.2042-3306.1998.tb04098.xgoogle scholar: lookup
            31. Geor RJ, Ommundson L, Fenton G, Pagan JD. Effects of an external nasal strip and frusemide on pulmonary haemorrhage in Thoroughbreds following high-intensity exercise.. Equine Vet J 2001 Nov;33(6):577-84.
              pubmed: 11720029doi: 10.2746/042516401776563490google scholar: lookup
            32. Weiss C, Seitel G, Bärtsch P. Coagulation and fibrinolysis after moderate and very heavy exercise in healthy male subjects.. Med Sci Sports Exerc 1998 Feb;30(2):246-51.
              pubmed: 9502353doi: 10.1097/00005768-199802000-00012google scholar: lookup
            33. Weiss DJ, Smith CM 2nd. Haemorrheological alterations associated with competitive racing activity in horses: implications for exercise-induced pulmonary haemorrhage (EIPH).. Equine Vet J 1998 Jan;30(1):7-12.
              pubmed: 9458393doi: 10.1111/j.2042-3306.1998.tb04082.xgoogle scholar: lookup
            34. Rawlings CA, Byars TD, Van Noy MK, Bisgard GE. Activated coagulation test in normal and heparinized ponies and horses.. Am J Vet Res 1975 May;36(5):711-3.
              pubmed: 1137216
            35. Kociba GJ, Bayly WM, Milne DW, Wigton DH, Gabel AA, Muir WW. Furosemide: effects on the hemostatic mechanism of resting and exercised standardbred horses.. Am J Vet Res 1984 Dec;45(12):2603-6.
              pubmed: 6524753
            36. Smith JE, Garbutt G, Lopes P, Tunstall Pedoe D. Effects of prolonged strenuous exercise (marathon running) on biochemical and haematological markers used in the investigation of patients in the emergency department.. Br J Sports Med 2004 Jun;38(3):292-4.
              pmc: PMC1724837pubmed: 15155430doi: 10.1136/bjsm.2002.002873google scholar: lookup
            37. Ferguson EW, Bernier LL, Banta GR, Yu-Yahiro J, Schoomaker EB. Effects of exercise and conditioning on clotting and fibrinolytic activity in men.. J Appl Physiol (1985) 1987 Apr;62(4):1416-21.
              pubmed: 3597218doi: 10.1152/jappl.1987.62.4.1416google scholar: lookup
            38. Matsumoto Y, Hayashi T, Hayakawa Y, Shinbo M, Niiya K, Sakuragawa N. Carbazochrome sodium sulphonate (AC-17) decreases the accumulation of tissue-type plasminogen activator in culture medium of human umbilical vein endothelial cells.. Blood Coagul Fibrinolysis 1995 May;6(3):233-8.
              pubmed: 7654937doi: 10.1097/00001721-199505000-00006google scholar: lookup
            39. Wagner PD, Gillespie JR, Landgren GL, Fedde MR, Jones BW, DeBowes RM, Pieschl RL, Erickson HH. Mechanism of exercise-induced hypoxemia in horses.. J Appl Physiol (1985) 1989 Mar;66(3):1227-33.
              pubmed: 2496088doi: 10.1152/jappl.1989.66.3.1227google scholar: lookup
            40. Vengust M, Staempfli H, Viel L, Heigenhauser G. Transvascular fluid flux from the pulmonary vasculature at rest and during exercise in horses.. J Physiol 2006 Jan 15;570(Pt 2):397-405.
              pmc: PMC1464305pubmed: 16269434doi: 10.1113/jphysiol.2005.098723google scholar: lookup
            41. Evans DL, Rose RJ. Cardiovascular and respiratory responses in Thoroughbred horses during treadmill exercise.. J Exp Biol 1988 Jan;134:397-408.
              pubmed: 3356965doi: 10.1242/jeb.134.1.397google scholar: lookup
            42. Bayly WM, Hodgson DR, Schulz DA, Dempsey JA, Gollnick PD. Exercise-induced hypercapnia in the horse.. J Appl Physiol (1985) 1989 Nov;67(5):1958-66.
              pubmed: 2513313doi: 10.1152/jappl.1989.67.5.1958google scholar: lookup
            43. Art T, Anderson L, Woakes AJ, Roberts C, Butler PJ, Snow DH, Lekeux P. Mechanics of breathing during strenuous exercise in Thoroughbred horses.. Respir Physiol 1990 Dec;82(3):279-94.
              pubmed: 2080318doi: 10.1016/0034-5687(90)90098-jgoogle scholar: lookup
            44. Christley RM, Hodgson DR, Evans DL, Rose RJ. Effects of training on the development of exercise-induced arterial hypoxemia in horses.. Am J Vet Res 1997 Jun;58(6):653-7.
              pubmed: 9185975
            45. Harkins JD, Hackett RP, Ducharme NG. Effect of furosemide on physiologic variables in exercising horses.. Am J Vet Res 1993 Dec;54(12):2104-9.
              pubmed: 8116946

            Citations

            This article has been cited 6 times.
            1. Bertram CA, Marzahl C, Bartel A, Stayt J, Bonsembiante F, Beeler-Marfisi J, Barton AK, Brocca G, Gelain ME, Gläsel A, Preez KD, Weiler K, Weissenbacher-Lang C, Breininger K, Aubreville M, Maier A, Klopfleisch R, Hill J. Cytologic scoring of equine exercise-induced pulmonary hemorrhage: Performance of human experts and a deep learning-based algorithm. Vet Pathol 2023 Jan;60(1):75-85.
              doi: 10.1177/03009858221137582pubmed: 36384369google scholar: lookup
            2. Dahlgren AR, Tablin F, Finno CJ. Genetics of equine bleeding disorders. Equine Vet J 2021 Jan;53(1):30-37.
              doi: 10.1111/evj.13290pubmed: 32463964google scholar: lookup
            3. Cacciani N, Salah H, Li M, Akkad H, Backeus A, Hedstrom Y, Jena BP, Bergquist J, Larsson L. Chaperone co-inducer BGP-15 mitigates early contractile dysfunction of the soleus muscle in a rat ICU model. Acta Physiol (Oxf) 2020 May;229(1):e13425.
              doi: 10.1111/apha.13425pubmed: 31799784google scholar: lookup
            4. Andreassen SM, Vinther AML, Nielsen SS, Andersen PH, Tnibar A, Kristensen AT, Jacobsen S. Changes in concentrations of haemostatic and inflammatory biomarkers in synovial fluid after intra-articular injection of lipopolysaccharide in horses. BMC Vet Res 2017 Jun 19;13(1):182.
              doi: 10.1186/s12917-017-1089-1pubmed: 28629364google scholar: lookup
            5. Hinchcliff KW, Couetil LL, Knight PK, Morley PS, Robinson NE, Sweeney CR, van Erck E. Exercise induced pulmonary hemorrhage in horses: American College of Veterinary Internal Medicine consensus statement. J Vet Intern Med 2015 May-Jun;29(3):743-58.
              doi: 10.1111/jvim.12593pubmed: 25996660google scholar: lookup
            6. Nagasawa H, Omori K, Ota S, Muramatsu KI, Takeuchi I, Ohsaka H, Yanagawa Y. Carbazochrome sodium sulfonate and tranexamic acid combination therapy to reduce blood transfusions after 24 h of injury: A retrospective study. Acute Med Surg 2024 Jan-Dec;11(1):e961.
              doi: 10.1002/ams2.961pubmed: 38715930google scholar: lookup
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