Effect of high intensity training on anaerobic capacity of middle gluteal muscle in Thoroughbred horses.
Abstract: We hypothesize that high intensity training for Thoroughbred horses that have been subjected to conventional training could further improve the metabolic properties of the middle gluteal muscle. Nine well-trained horses were subjected to high intensity (80-100% Vdot;O(2)max, 5 minx2) training for 12 weeks. Biopsy samples were obtained from the muscle before and after 4 and 12 weeks of training. Three of the 9 horses did not complete the training programme. In the remaining 6 horses, activities of succinic dehydrogenase (SDH), phosphofructokinase (PFK) and 3-hydroxy acyl CoA dehydrogenase (HAD), and the composition of myosin heavy chain isoforms were analyzed by biochemical techniques. After 12 weeks of training, a significant increase was found in PFK activity but not in the SDH and HAD activities. There were no significant changes in the composition of myosin heavy chain isoforms. The high intensity training in this study was effective at increasing glycolytic enzyme activity, indicating the possibility to improve anaerobic capacity, which potentially could contribute greatly to performance in Thoroughbred horses. This study also highlighted a fact that high intensity training should be given with the great care to prevent the skeletal muscle injuries.
Publication Date: 2003-12-16 PubMed ID: 14672857DOI: 10.1016/j.rvsc.2003.08.010Google Scholar: Lookup
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- Journal Article
- Aerobic Capacity
- Anaerobic Metabolism
- Animal Studies
- Athletic Performance
- Biochemistry
- Enzymes
- Equine Health
- Equine Science
- Equine Studies
- Exercise
- Exercise Physiology
- Glycolysis
- High-performance Liquid Chromatography (HPLC)
- Horse Training
- Horses
- Metabolism
- Muscle Fiber Types
- Musculoskeletal System
- Thoroughbreds
- Veterinary Science
Summary
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The research looks at how high intensity training could enhance the metabolic properties of a specific muscle in Thoroughbred horses. The results suggest that high intensity training can increase anaerobic capacity, potentially improving performance, though it must be carefully managed to avoid muscle injuries.
Research Methodology
- The study involved nine Thoroughbred horses that had already been subjected to conventional training.
- These horses were put through high intensity training for 12 weeks. The training intensity was between 80-100% Vdot;O(2)max, for 5 minutes of exercise, repeated twice.
- Before and after 4 and 12 weeks of training, muscle biopsy samples were taken from the horses. These samples came from the middle gluteal muscle, a large muscle in the hip region of the horse.
- However, three of the nine horses were unable to complete the training program.
Analysis and Results
- The researchers used biochemical techniques to analyze the activities of three enzymes in the muscle samples – succinic dehydrogenase (SDH), phosphofructokinase (PFK) and 3-hydroxy acyl CoA dehydrogenase (HAD) – as well as the composition of myosin heavy chain isoforms (different types of a protein that plays a major role in muscle contraction).
- After 12 weeks of high intensity training, PFK activity significantly increased in the muscle samples. However, there were no significant changes in SDH and HAD activity levels.
- The composition of myosin heavy chain isoforms in the muscle didn’t significantly change either.
- The increased activity of the PFK enzyme implies that the high intensity training was effective at boosting glycolytic enzyme activity. This boost indicates an improvement in anaerobic capacity, the ability of the muscle to work hard in the absence of oxygen, which can greatly enhance performance in racehorses.
Cautionary Note
- Despite these promising results, the study states that such high intensity training must be implemented with great care to prevent skeletal muscle injuries. This is likely because the intense nature of the training puts significant strain on the horse’s muscles, which can lead to injury if not properly managed.
Conclusions
- The study concludes that high intensity training can be beneficial for Thoroughbred horses’ performance by enhancing the metabolic properties of the middle gluteal muscle. However, it also draws attention to the need for careful monitoring and management of the training intensity to prevent skeletal muscle injuries.
Cite This Article
APA
Eto D, Yamano S, Mukai K, Sugiura T, Nasu T, Tokuriki M, Miyata H.
(2003).
Effect of high intensity training on anaerobic capacity of middle gluteal muscle in Thoroughbred horses.
Res Vet Sci, 76(2), 139-144.
https://doi.org/10.1016/j.rvsc.2003.08.010 Publication
Researcher Affiliations
- Equine Research Institute, Japan Racing Association, 321-4 Tokami-cho, Utsunomiya, 320-0856, Tochigi, Japan.
MeSH Terms
- 3-Hydroxyacyl CoA Dehydrogenases / metabolism
- Animals
- Biopsy / veterinary
- Female
- Horses / physiology
- Lactic Acid / blood
- Male
- Muscle, Skeletal / enzymology
- Muscle, Skeletal / physiology
- Myosin Heavy Chains / metabolism
- Phosphofructokinases / metabolism
- Physical Conditioning, Animal / physiology
- Protein Isoforms
- Succinate Dehydrogenase / metabolism
Citations
This article has been cited 10 times.- Darbandi H, Munsters C, Parmentier J, Havinga P. Detecting fatigue of sport horses with biomechanical gait features using inertial sensors.. PLoS One 2023;18(4):e0284554.
- Mukai K, Kitaoka Y, Takahashi Y, Takahashi T, Takahashi K, Ohmura H. Moderate-intensity training in hypoxia improves exercise performance and glycolytic capacity of skeletal muscle in horses.. Physiol Rep 2021 Dec;9(23):e15145.
- de Meeûs d'Argenteuil C, Boshuizen B, Oosterlinck M, van de Winkel D, De Spiegelaere W, de Bruijn CM, Goethals K, Vanderperren K, Delesalle CJG. Flexibility of equine bioenergetics and muscle plasticity in response to different types of training: An integrative approach, questioning existing paradigms.. PLoS One 2021;16(4):e0249922.
- Wang W, Mukai K, Takahashi K, Ohmura H, Takahashi T, Hatta H, Kitaoka Y. Short-term hypoxic training increases monocarboxylate transporter 4 and phosphofructokinase activity in Thoroughbreds.. Physiol Rep 2020 Jun;8(11):e14473.
- Best R, Standing R. The Spatiotemporal Characteristics of 0-24-Goal Polo.. Animals (Basel) 2019 Jul 16;9(7).
- Bryan K, McGivney BA, Farries G, McGettigan PA, McGivney CL, Gough KF, MacHugh DE, Katz LM, Hill EW. Equine skeletal muscle adaptations to exercise and training: evidence of differential regulation of autophagosomal and mitochondrial components.. BMC Genomics 2017 Aug 9;18(1):595.
- Kang OD, Park YS. Effect of age on heart rate, blood lactate concentration, packed cell volume and hemoglobin to exercise in Jeju crossbreed horses.. J Anim Sci Technol 2017;59:2.
- Chanda M, Srikuea R, Cherdchutam W, Chairoungdua A, Piyachaturawat P. Modulating effects of exercise training regimen on skeletal muscle properties in female polo ponies.. BMC Vet Res 2016 Nov 4;12(1):245.
- Yamano S, Kawai M, Minami Y, Hiraga A, Miyata H. Differences in Muscle Fiber Recruitment Patterns between Continuous and Interval Exercises.. J Equine Sci 2010;21(4):59-65.
- McGivney BA, McGettigan PA, Browne JA, Evans AC, Fonseca RG, Loftus BJ, Lohan A, MacHugh DE, Murphy BA, Katz LM, Hill EW. Characterization of the equine skeletal muscle transcriptome identifies novel functional responses to exercise training.. BMC Genomics 2010 Jun 23;11:398.
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