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Animals : an open access journal from MDPI2021; 11(9); doi: 10.3390/ani11092504

Effect of Overfeeding Shetland Pony Mares on Embryonic Glucose and Lipid Accumulation, and Expression of Imprinted Genes.

Abstract: Maternal overfeeding is associated with disturbances in early embryonic epigenetic reprogramming, leading to altered expression of imprinted genes and nutrient transporters, which can affect both fetal and placental development and have lasting effects on the health of resulting offspring. To examine how maternal overfeeding affects the equine embryo, Shetland pony mares were fed either a high-energy (HE: 200% of net energy requirements) or maintenance (control) diet. Mares from both groups were inseminated, and day-seven embryos were recovered and transferred to recipients from the same or the alternate group. The expression of a panel of imprinted genes, glucose and amino acid transporters, and DNA methyltransferases (DNMTs) were determined in conceptus membranes after recovery on day 28 of gestation (late pre-implantation phase). The expression of nutrient transporters was also assessed in endometrium recovered from recipient mares immediately after conceptus removal. In addition, glucose uptake by day-28 extra-embryonic membranes, and lipid droplet accumulation in day-seven blastocysts were assessed. Maternal overfeeding resulted in elevated expression of imprinted genes (IGF2, IGF2R, H19, GRB10, PEG10 and SNRPN), DNMTs (DNMT1 and DNMT3B), glucose (SLC2A1), fructose (SLC2A5) and amino acid (SLC7A2) transporters following ET from an HE to a control mare. Expression of amino acid transporters (SLC1A5 and SLC7A1) was also elevated in the endometrium after ET from HE to control. Maternal overfeeding did not affect lipid droplet accumulation in blastocysts, or glucose uptake by day-28 membranes. It remains to be seen whether the alterations in gene expression are maintained throughout gestation and into postnatal life.
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Publication Date: 2021-08-26 PubMed ID: 34573470PubMed Central: PMC8470267DOI: 10.3390/ani11092504Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied how overfeeding Shetland pony mares affects the expression of genetic material and nutrient accumulation in early-stage embryos. The results showed that overfeeding led to elevated expression of certain genes and nutrient transporters, but it did not affect the accumulation of fat in the embryos or glucose uptake by the embryos’ membranes.

Research Objective and Methodology

  • The objective of this study was to understand how maternal overfeeding influences the equine embryo, especially in terms of epigenetic reprogramming – the process by which gene expression is altered without changing the DNA sequence.
  • To achieve this, Shetland pony mares were split into two dietary groups: those receiving a high-energy (200% of net energy requirements) diet, and those receiving a maintenance (control) diet.
  • Mares from both groups were inseminated, and embryos from the seventh day were collected and transferred to recipients within the same or different dietary group.

Data Collection

  • On the 28th day of gestation, the expression of a set of imprinted genes, glucose and amino acid transporters, and DNA methyltransferases (enzymes that transfer methyl groups to DNA molecules) were measured in the conceptuses’ membranes.
  • The study team noted nutrient transporters expression in the endometrium (inner lining of the uterus) after the conceptus was removed.
  • They also measured glucose uptake by the extra-embryonic membranes on day 28, and fat accumulation in the blastocysts (early-stage embryos) on day seven.

Findings

  • Overfeeding led to increased expression of imprinted genes, DNA methyltransferases, and glucose, fructose, and amino acid transporters when embryos were transferred from a high-energy diet mare to a control diet mare.
  • No noticeable effects were found on fat accumulation in day seven blastocysts or glucose uptake by the membranes on day 28 due to overfeeding.

Conclusion

  • Maternal overfeeding impacts the genetic and nutrient transport expressions in horse embryos, potentially influencing their development and the health of the resulting offspring.
  • The study doesn’t confirm if these changes in genetic expression persist throughout gestation or into postnatal life, indicating a direction for future research.

Cite This Article

APA
D' Fonseca NMM, Gibson CME, van Doorn DA, Roelfsema E, de Ruijter-Villani M, Stout TAE. (2021). Effect of Overfeeding Shetland Pony Mares on Embryonic Glucose and Lipid Accumulation, and Expression of Imprinted Genes. Animals (Basel), 11(9). https://doi.org/10.3390/ani11092504

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 9

Researcher Affiliations

D' Fonseca, Nicky M M
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
Gibson, Charlotte M E
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
van Doorn, David A
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
  • Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands.
Roelfsema, Ellen
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
de Ruijter-Villani, Marta
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.
Stout, Tom A E
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands.

Grant Funding

  • 317146 / FP7 People: Marie-Curie Actions

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 2 times.
  1. Li T, Jin Y, Wu J, Ren Z. Beyond energy provider: multifunction of lipid droplets in embryonic development. Biol Res 2023 Jul 12;56(1):38.
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