Effect of sodium hyaluronate in collagenase-induced superficial digital flexor tendinitis in horses.
Abstract: Superficial digital flexor tendinitis was induced in each forelimb of 8 horses by injecting 4,000 U of collagenase into the midmetacarpal region of the tendon. In each horse, each tendon was treated 24 and 96 hours after the collagenase injection with SC injections of sodium hyaluronate (treated limbs) or an equal volume of 0.9% NaCl solution (control limbs). Exercise was restricted for the first 3 weeks of the study, and a controlled exercise program was instituted for the remainder of the study. Horses were evaluated clinically for lameness, tendon swelling, and midmetacarpal limb circumference. Ultrasonographic examinations were performed regularly (11 examinations/horse) throughout the study, and all horses were euthanatized 12 weeks after collagenase injections. Tendons from 4 horses were harvested for biomechanical testing, and samples were obtained from tendons from the remaining 4 horses for biochemical analysis of collagen. Samples were obtained from all tendons for microscopic evaluation. Significant differences between treated and control tendons were not noticed in any of the variables examined in live horses, although trends toward less lameness in treated limbs and toward better healing on ultrasonographic examination in control limbs were recorded. Significant differences were not noticed in biomechanical or biochemical evaluations, and the only significant (P < 0.05) microscopic finding was more severe inflammation in tendons from treated limbs. This study did not reveal significant benefits of treatment with sodium hyaluronate outside a synovial sheath on tendon repair in collagenase-induced tendinitis.
Publication Date: 1992-12-01 PubMed ID: 1476324
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research was aimed at discerning the impact of sodium hyaluronate on collagenase-induced tendinitis in horses’s superficial digital flexor tendons. However, the study found no significant difference in terms of healing or improvements due to sodium hyaluronate treatment.
Objective and Methodology
- The research studied the impact of sodium hyaluronate on collagenase-induced superficial digital flexor tendinitis in horses. Eight horses’ forelimbs were subjected to this tendinitis by injecting collagenase into the midmetacarpal area of their tendons.
- Following this, each horse received either a treatment of sodium hyaluronate or a control solution 24 and 96 hours post the collagenase injection. Exercise was kept under control for the first three weeks, after which a regulated workout program was started.
Observations and Evaluations
- Horses’ clinical evaluations included checking for lameness, swelling, and the circumference of the midmetacrpel limb. Frequent Ultrasonographic exams were also conducted during the study, after which all horses were euthanized 12 weeks post collagenase injections.
- Post euthanization, tendons from four horses were chosen for biomechanical testing and four for biochemical analysis. All tendons underwent microscopic evaluations.
Findings
- There were no significant differences noted between the treated horses and the control group when considering variables tested on live horses. Meanwhile, there seemed to be a trend of lesser lameness in the treated horses and better healing outcomes using ultrasonographic analysis in the control horses.
- Similarly, there were no noticeable differences in biomechanical or biochemical evaluations. The only significant microscopic finding was the presence of severer inflammation in tendons from treated limbs.
- The findings suggest that treating with sodium hyaluronate outside a synovial sheath does not show considerable benefits on tendon repair in collagenase-induced tendinitis.
Cite This Article
APA
Foland JW, Trotter GW, Powers BE, Wrigley RH, Smith FW.
(1992).
Effect of sodium hyaluronate in collagenase-induced superficial digital flexor tendinitis in horses.
Am J Vet Res, 53(12), 2371-2376.
Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
MeSH Terms
- Animals
- Biomechanical Phenomena
- Collagen / drug effects
- Collagen / metabolism
- Collagenases
- Horse Diseases / chemically induced
- Horse Diseases / drug therapy
- Horse Diseases / pathology
- Horses
- Hyaluronic Acid / therapeutic use
- Tendinopathy / chemically induced
- Tendinopathy / drug therapy
- Tendinopathy / pathology
- Tendinopathy / veterinary
- Tendons / diagnostic imaging
- Tendons / drug effects
- Tendons / metabolism
- Tendons / pathology
- Ultrasonography
Citations
This article has been cited 11 times.- Marr N, Meeson R, Kelly EF, Fang Y, Peffers MJ, Pitsillides AA, Dudhia J, Thorpe CT. CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4.. Int J Mol Sci 2021 Sep 8;22(18).
- Dincel YM, Adanir O, Arikan Y, Caglar AK, Dogru SC, Arslan YZ. EFFECTS OF HIGH-DOSE VITAMIN C AND HYALURONIC ACID ON TENDON HEALING.. Acta Ortop Bras 2018 Mar-Apr;26(2):82-85.
- Vandenberghe A, Broeckx SY, Beerts C, Seys B, Zimmerman M, Verweire I, Suls M, Spaas JH. Tenogenically Induced Allogeneic Mesenchymal Stem Cells for the Treatment of Proximal Suspensory Ligament Desmitis in a Horse.. Front Vet Sci 2015;2:49.
- Abate M, Schiavone C, Salini V. The use of hyaluronic acid after tendon surgery and in tendinopathies.. Biomed Res Int 2014;2014:783632.
- Shepherd JH, Screen HR. Fatigue loading of tendon.. Int J Exp Pathol 2013 Aug;94(4):260-70.
- O'Brien EJ, Frank CB, Shrive NG, Hallgrímsson B, Hart DA. Heterotopic mineralization (ossification or calcification) in tendinopathy or following surgical tendon trauma.. Int J Exp Pathol 2012 Oct;93(5):319-31.
- Spaas JH, Guest DJ, Van de Walle GR. Tendon regeneration in human and equine athletes: Ubi Sumus-Quo Vadimus (where are we and where are we going to)?. Sports Med 2012 Oct 1;42(10):871-90.
- Fu SC, Rolf C, Cheuk YC, Lui PP, Chan KM. Deciphering the pathogenesis of tendinopathy: a three-stages process.. Sports Med Arthrosc Rehabil Ther Technol 2010 Dec 13;2:30.
- Ozgen M, Fırat S, Sarsan A, Topuz O, Ardıç F, Baydemir C. Short- and long-term results of clinical effectiveness of sodium hyaluronate injection in supraspinatus tendinitis.. Rheumatol Int 2012 Jan;32(1):137-44.
- Taguchi M, Zhao C, Sun YL, Jay GD, An KN, Amadio PC. The effect of surface treatment using hyaluronic acid and lubricin on the gliding resistance of human extrasynovial tendons in vitro.. J Hand Surg Am 2009 Sep;34(7):1276-81.
- Zhao C, Sun YL, Amadio PC, Tanaka T, Ettema AM, An KN. Surface treatment of flexor tendon autografts with carbodiimide-derivatized hyaluronic Acid. An in vivo canine model.. J Bone Joint Surg Am 2006 Oct;88(10):2181-91.
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