Effects of amnion and live yeast cell derivative on second-intention healing in horses.
Abstract: Equine amnion and live yeast cell derivative were evaluated as a biological dressing and as a topical wound medicament respectively, in the treatment of granulating wounds of the distal portion of the limbs of horses. Wounds measuring 2.5 x 2.5 cm were created on the dorsomedial aspect of the metacarpal and metatarsal region of all 4 limbs of 9 horses. Each wound was assigned randomly to 1 of 3 treatment groups: group C, treated with a nonadherent bandage as a control; group A, treated with amnion beneath a nonadherent bandage; and group L, treated with live yeast cell derivative beneath a nonadherent bandage. Rates of contraction and epithelialization were not significantly different among groups. Percentage of epithelialization for group A was significantly greater throughout the study than for the other groups. Number of days to complete healing was significantly less in group A and significantly greater in group L than in group C. Incidence and severity of exuberant granulation tissue were significantly less in group A than in the other 2 groups. Group L had more frequent and severe cases of exuberant granulation tissue than the other 2 groups.
Publication Date: 1991-08-01 PubMed ID: 1928923
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research article investigates the effects of horse amnion and live yeast cell derivative on the healing process of wounds on horses’ lower limbs. The article reveals that using horse amnion showed sped up healing and a lower incidence of overgrowth of granulation tissue.
Experimental Design
- The researchers created controlled wounds of size 2.5 x 2.5 cm on the dorsomedial aspect of the legs of nine horses, ensuring that each horse had a wound on all four legs.
- These wounds were randomly assigned to three different treatment groups: Control group (C), Amnion group (A), and Live yeast cell derivative group (L).
- The control group got treated with a nonadherent bandage. The Amnion group had the amnion beneath a nonadherant bandage while the live yeast cell derivative group was bandaged with the yeast derivative beneath a nonadherent bandage.
Outcome Measures
- Key metrics: the percentage of closed wound surface (epithelialization), resultant scar and overall healing days, appearance, and severity of extra granulation tissue (growth of new tiny blood vessels).
- The comparisons were made among all treatment groups to figure out the best technique.
Findings
- The results showed no considerable difference in wound contraction and epithelialization rates among all groups; however, the percentage of epithelialization was significantly higher in the amnion group throughout the study.
- Amnion group had a significantly lower healing time as compared to the control group.
- Notably, the amnion treatment also led to lower instances and severities of exuberant granulation tissue (hypergranulation), which can impede the healing process. In contrast, the group treated with live yeast cell derivative experienced a higher rate of such tissue formation, which may have resulted in a comparatively more extended healing period.
- Overall, it indicates that the application of amnion as a biological dressing can potentially enhance the healing of wounds in horses.
Cite This Article
APA
Bigbie RB, Schumacher J, Swaim SF, Purohit RC, Wright JC.
(1991).
Effects of amnion and live yeast cell derivative on second-intention healing in horses.
Am J Vet Res, 52(8), 1376-1382.
Publication
Researcher Affiliations
- Department of Large Animal Surgery and Medicine, College of Veterinary Medicine, Auburn University, AL 36849-5522.
MeSH Terms
- Amnion
- Animals
- Biological Dressings / veterinary
- Biological Products
- Epithelium / physiology
- Female
- Granulation Tissue / physiology
- Horses / injuries
- Male
- Peptide Fragments
- Peptides
- Random Allocation
- Saccharomyces cerevisiae
- Wound Healing
Citations
This article has been cited 12 times.- Rosa MVD, Rosa M, Botteon PTL. Cryopreserved equine amniotic membrane and its use in cutaneous wounds of horses. Braz J Vet Med 2022;44:e003122.
- Kumar S, Kumar A, Kumar N, Singh P, Singh TU, Singh BR, Gupta PK, Thakur VK. In vivo therapeutic efficacy of Curcuma longa extract loaded ethosomes on wound healing. Vet Res Commun 2022 Dec;46(4):1033-1049.
- Ibrahim A, Hassan D, Kelany N, Kotb S, Soliman M. Validation of Three Different Sterilization Methods of Tilapia Skin Dressing: Impact on Microbiological Enumeration and Collagen Content. Front Vet Sci 2020;7:597751.
- Laurent A, Hirt-Burri N, Scaletta C, Michetti M, de Buys Roessingh AS, Raffoul W, Applegate LA. Holistic Approach of Swiss Fetal Progenitor Cell Banking: Optimizing Safe and Sustainable Substrates for Regenerative Medicine and Biotechnology. Front Bioeng Biotechnol 2020;8:557758.
- Dubuc V, Laverty S, Richard H, Doré M, Theoret C. Development of a computer-based quantification method for immunohistochemically-stained tissues and its application to study mast cells in equine wound healing (proof of concept). BMC Vet Res 2020 Jul 2;16(1):228.
- Tsang AS, Dart AJ, Biasutti SA, Jeffcott LB, Smith MM, Little CB. Effects of tendon injury on uninjured regional tendons in the distal limb: An in-vivo study using an ovine tendinopathy model. PLoS One 2019;14(4):e0215830.
- Kamus LJ, Theoret C, Costa MC. Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses. PLoS One 2018;13(11):e0206989.
- Spaas JH, Broeckx S, Van de Walle GR, Polettini M. The effects of equine peripheral blood stem cells on cutaneous wound healing: a clinical evaluation in four horses. Clin Exp Dermatol 2013 Apr;38(3):280-4.
- Akela A, Nandi SK, Das P, Banerjee D, Roy S, Datta U. Autologous bone marrow-derived cells with placental extract for healing excisional cutaneous wounds in animal model. Int Wound J 2013 Apr;10(2):167-76.
- Akela A, Nandi SK, Banerjee D, Das P, Roy S, Joardar SN, Mandal M, Das PK, Pradhan NR. Evaluation of autologous bone marrow in wound healing in animal model: a possible application of autologous stem cells. Int Wound J 2012 Oct;9(5):505-16.
- Borena BM, Pawde AM, Amarpal, Aithal HP, Kinjavdekar P, Singh R, Kumar D. Evaluation of autologous bone marrow-derived nucleated cells for healing of full-thickness skin wounds in rabbits. Int Wound J 2010 Aug;7(4):249-60.
- Knezevic V. Differentiation potential of rat amnion. J Anat 1996 Aug;189 ( Pt 1)(Pt 1):1-7.
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