Effects of bilayer gelatin/β-tricalcium phosphate sponges loaded with mesenchymal stem cells, chondrocytes, bone morphogenetic protein-2, and platelet rich plasma on osteochondral defects of the talus in horses.
Abstract: Osteochondrosis (OC) is a common and clinically important joint disorder in horses. However, repair of the OC region is difficult because of the avascular nature of cartilage. This study aimed to evaluate the efficacy of bilayer gelatin/β-tricalcium phosphate (GT) sponges loaded with mesenchymal stem cells (MSCs), chondrocytes, bone morphogenetic protein-2 (BMP-2), and platelet rich plasma (PRP) for the repair of osteochondral defects of the talus in horses. Full-thickness osteochondral defects were created on both the lateral trochlear ridges of the talus (n = 6). In the test group, a basic GT sponge loaded with MSCs and BMP-2 (MSC/BMP2/GT) was inserted into the lower part of the defect, and an acidic GT sponge loaded with chondrocyte, MSCs, and PRP (Ch/MSC/PRP/GT) was inserted into the upper part of the defect. In the control group, the defect was treated only with bilayer GT sponges. Repair of osteochondral defects was assessed by radiography, quantitative computed tomography (QCT), and macroscopic and histological evaluation. The test group showed significantly higher radiographic, QCT, macroscopic, and histological scores than the control group. This study demonstrated that the bilayer scaffolds consisting of Ch/MSC/PRP/GT for the chondrogenic layer and MSC/BMP2/GT for the osteogenic layer promoted osteochondral regeneration in an equine model. The bilayer scaffolds described here may be useful for treating horses with OC.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication Date: 2013-09-05 PubMed ID: 24054973DOI: 10.1016/j.rvsc.2013.08.016Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study aimed to assess the efficacy of using bi-layer gelatin and β-tricalcium phosphate sponges loaded with mesenchymal stem cells, chondrocytes, bone morphogenetic protein-2, and platelet rich plasma in healing joint defects in horses. The research showed that these loaded sponges significantly improved repair over using sponges alone, suggesting a new method for treating a common joint disorder in horses.
Research Objectives and Methodology
The research aimed to determine the efficacy of a bilayer gelatin/β-tricalcium phosphate sponge, loaded with mesenchymal stem cells (MSCs), chondrocytes, bone morphogenetic protein-2 (BMP-2), and platelet rich plasma (PRP), in repairing osteochondral defects in horses.
- The study involves a comparison between a test group and a control group.
- Osteochondral defects were manually created on the test group horses’ taluses.
- In the test group, the sponge was filled with MSCs and BMP-2 (MSC/BMP2/GT) and inserted into the lower part of the defect.
- An acidic GT sponge filled with chondrocyte, MSCs, and PRP (Ch/MSC/PRP/GT) was inserted into the upper part of the defect.
- In contrast, the control group’s defects were only treated with bilayer GT sponges.
Results and Assessments
The researchers assessed the repair of the osteochondral defects through various techniques.
- These evaluation methods included radiography, quantitative computed tomography (QCT), macroscopic, and histological evaluation.
- The research found that the test group displayed significantly higher success rates in the radiographic, QCT, macroscopic, and histological scores compared to the control group.
- These results suggest that the osteochondral regeneration in the test group was promoted by using the bilayer scaffolds.
- The bilayer scaffolds consisted of one layer for chondrocyte promotion (Ch/MSC/PRP/GT) and another for osteogenic (bone formation) promotion (MSC/BMP2/GT).
Conclusion
From the findings, the researchers concluded that the bilayer scaffolds method could be useful in treating horses with osteochondrosis. The study demonstrates that the loaded sponges resulted in a significantly improved repair of the osteochondral defects over using the sponges alone. Thus, this innovation could present a new treatment option for this common and important joint disorder in horses.
Cite This Article
APA
Seo JP, Tanabe T, Tsuzuki N, Haneda S, Yamada K, Furuoka H, Tabata Y, Sasaki N.
(2013).
Effects of bilayer gelatin/β-tricalcium phosphate sponges loaded with mesenchymal stem cells, chondrocytes, bone morphogenetic protein-2, and platelet rich plasma on osteochondral defects of the talus in horses.
Res Vet Sci, 95(3), 1210-1216.
https://doi.org/10.1016/j.rvsc.2013.08.016 Publication
Researcher Affiliations
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido 080-8555, Japan.
MeSH Terms
- Animals
- Bone Morphogenetic Protein 2 / therapeutic use
- Calcium Phosphates / therapeutic use
- Chondrocytes / transplantation
- Female
- Gelatin Sponge, Absorbable / therapeutic use
- Horse Diseases / pathology
- Horse Diseases / therapy
- Horses / surgery
- Mesenchymal Stem Cell Transplantation / methods
- Mesenchymal Stem Cell Transplantation / veterinary
- Osteochondrosis / pathology
- Osteochondrosis / therapy
- Osteochondrosis / veterinary
- Platelet-Rich Plasma / physiology
- Surgical Sponges / veterinary
- Talus / pathology
- Talus / surgery
Citations
This article has been cited 10 times.- Wu S, Guo W, Li R, Zhang X, Qu W. Progress of Platelet Derivatives for Cartilage Tissue Engineering. Front Bioeng Biotechnol 2022;10:907356.
- Fu JN, Wang X, Yang M, Chen YR, Zhang JY, Deng RH, Zhang ZN, Yu JK, Yuan FZ. Scaffold-Based Tissue Engineering Strategies for Osteochondral Repair. Front Bioeng Biotechnol 2021;9:812383.
- Hashemi S, Amirabad LM, Nazhvani FD, Zarrintaj P, Namazi H, Saadatfar A, Golchin A. Bilayer Scaffolds for Interface Tissue Engineering and Regenerative Medicine: A Systematic Reviews. Adv Exp Med Biol 2021;1347:83-113.
- Chung MJ, Son JY, Park S, Park SS, Hur K, Lee SH, Lee EJ, Park JK, Hong IH, Kim TH, Jeong KS. Mesenchymal Stem Cell and MicroRNA Therapy of Musculoskeletal Diseases. Int J Stem Cells 2021 May 30;14(2):150-167.
- Scarfì S. Use of bone morphogenetic proteins in mesenchymal stem cell stimulation of cartilage and bone repair. World J Stem Cells 2016 Jan 26;8(1):1-12.
- Li X, Ding J, Wang J, Zhuang X, Chen X. Biomimetic biphasic scaffolds for osteochondral defect repair. Regen Biomater 2015 Sep;2(3):221-8.
- Ham O, Lee CY, Kim R, Lee J, Oh S, Lee MY, Kim J, Hwang KC, Maeng LS, Chang W. Therapeutic Potential of Differentiated Mesenchymal Stem Cells for Treatment of Osteoarthritis. Int J Mol Sci 2015 Jul 2;16(7):14961-78.
- Qu D, Mosher CZ, Boushell MK, Lu HH. Engineering complex orthopaedic tissues via strategic biomimicry. Ann Biomed Eng 2015 Mar;43(3):697-717.
- Samorezov JE, Alsberg E. Spatial regulation of controlled bioactive factor delivery for bone tissue engineering. Adv Drug Deliv Rev 2015 Apr;84:45-67.
- Grässel S, Lorenz J. Tissue-engineering strategies to repair chondral and osteochondral tissue in osteoarthritis: use of mesenchymal stem cells. Curr Rheumatol Rep 2014 Oct;16(10):452.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
