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Equine veterinary journal2020; 53(3); 618-627; doi: 10.1111/evj.13300

Effects of endurance racing on horse plasma extracellular particle miRNA.

Abstract: Physical exercise is an essential factor in preventing and treating metabolic diseases by promoting systemic benefits throughout the body. The molecular factors involved in this process are poorly understood. Micro RNAs (miRNAs) are small non-coding RNAs that inhibit mRNA transcription. MiRNAs, which can participate in the benefits of exercise to health, circulate in plasma in extracellular particles (EP). Horses that undergo endurance racing are an excellent model to study the impact of long-duration/low intensity exercise in plasma EP miRNAs. Objective: To evaluate the effects of 160 km endurance racing on horse plasma extracellular particles and their miRNA population. Methods: Cohort study. Methods: We collected plasma from five Arabian horses during five time-points of an endurance ride. Extracellular particles were purified from plasma and characterised by electron microscopy, resistive pulse sensing (qNano) and western blotting. Small RNAs were purified from horse plasma EP, and sequencing was performed. Results: Endurance racing increased EP concentration and average diameter compared to before the race. Western blotting showed a high concentration of extracellular vesicles proteins 2 hours after the race, which returned to baseline 15 hours after the race. MicroRNA differential expression analysis revealed increasing levels of eca-miR-486-5p during and after the race, and decreasing levels of eca-miR-9083 after the end. Conclusions: This study adds new data about the variation in plasma EP concentrations after long-distance exercise and brings new insights about the roles of exercise-derived EP miRNAs during low-intensity endurance exercise.
© 2020 EVJ Ltd.
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Publication Date: 2020-07-01 PubMed ID: 32484928DOI: 10.1111/evj.13300Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how long-distance, low-intensity exercise, specifically 160 km endurance racing in Arabian horses, affects both the quantity and characteristics of microscopic particles in horse plasma called extracellular particles (EPs) and their contained microRNAs (miRNAs). MiRNAs are small molecular components that perform a regulatory function in the body. The study suggests that endurance racing elevates EP concentration, influences their size, and alters the expression of certain miRNAs, providing new insights into the impacts of such exercise on horse health.

Objective and Methods

  • The primary objective of the research was to understand the effects of long-distance endurance racing on the plasma EP population, specifically the miRNA content, in horses.
  • A cohort of five Arabian horses was studied across five different phases of endurance riding.
  • Plasma samples were collected from the horses, from which extracellular particles were extracted and then analysed using techniques like electron microscopy, resistive pulse sensing (qNano), and western blotting.
  • Small miRNAs were isolated from horse plasma EPs and sequenced for further analysis.

Results

  • The study found that endurance racing led to an increase in the concentration and average size of EPs compared to their levels before the race.
  • Western blotting, a technique used to detect specific proteins, revealed a high concentration of extracellular vesicle proteins two hours post-race, which returned to normal approximately 15 hours after the race ended.
  • miRNA expression analysis showed an increase in eca-miR-486-5p, a specific kind of miRNA, during and after the race, while the levels of another miRNA, eca-miR-9083, went down after the race concluded.

Conclusion

  • The research presents valuable data on how long-distance exercise can impact the concentration of plasma EPs.
  • It also offers new insights on how the miRNAs contained within these EPs vary during low-intensity endurance exercise, potentially contributing to our broader understanding of exercise’s health benefits.

Cite This Article

APA
de Oliveira GP, Porto WF, Palu CC, Pereira LM, Reis AMM, Marçola TG, Teixeira-Neto AR, Franco OL, Pereira RW. (2020). Effects of endurance racing on horse plasma extracellular particle miRNA. Equine Vet J, 53(3), 618-627. https://doi.org/10.1111/evj.13300

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 53
Issue: 3
Pages: 618-627

Researcher Affiliations

de Oliveira, Getúlio P
  • Programa de pós-graduação em Patologia Molecular, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
  • Division of Allergy and Inflammation, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Porto, William F
  • Programa de pós-graduação em Biotecnologia, S-Inova Biotech, Universidade Católica Dom Bosco-UCDB, Campo Grande, MS, Brasil.
Palu, Cintia C
  • NSilico Life Science LTDA, Unit 1.23, Nova Center, Belfield Innovation Park, Dublin, Ireland.
  • University College Cork, Cork, Ireland.
Pereira, Lydyane M
  • Programa de pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil.
Reis, Alessandra M M
  • Programa de pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil.
Marçola, Tatiana G
  • Programa de pós-graduação em Saúde Animal, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
Teixeira-Neto, Antonio R
  • Programa de pós-graduação em Saúde Animal, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
Franco, Octavio L
  • Programa de pós-graduação em Patologia Molecular, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
  • Programa de pós-graduação em Biotecnologia, S-Inova Biotech, Universidade Católica Dom Bosco-UCDB, Campo Grande, MS, Brasil.
  • Programa de pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil.
  • Programa de pós-graduação em Educação Física, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil.
Pereira, Rinaldo W
  • Programa de pós-graduação em Patologia Molecular, Universidade de Brasília-UnB, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, DF, Brasil.
  • Programa de pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil.
  • Programa de pós-graduação em Educação Física, Universidade Católica de Brasília-UCB, Brasília, DF, Brasil.

MeSH Terms

  • Animals
  • Cohort Studies
  • Horses
  • MicroRNAs / genetics
  • Physical Conditioning, Animal
  • Physical Endurance
  • Plasma

Grant Funding

  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Fundação de Apoio à Pesquisa do Distrito Federal

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Citations

This article has been cited 9 times.
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