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Home / Equine Research Bank / Vet World / Effects of four extenders on the quality of frozen semen in ...
Veterinary world2019; 12(1); 34-40; doi: 10.14202/vetworld.2019.34-40

Effects of four extenders on the quality of frozen semen in Arabian stallions.

Abstract: Different types of extenders have a variety of components which show the tolerance effect on sperm protection during freezing procedures. In the present study, we have examined the impact of the extenders HF-20 and Tris, which were locally manufactured, and they are competing with commercial extenders INRA Freeze® (IMV Technologies, France) and EquiPlus Freeze® (Minitube, Germany) on the quality of horses frozen semen. Methods: A total of 15 ejaculates from three healthy stallions were collected and cryopreserved in the same environment. Each semen sample collected was divided into four equal parts and processed. All samples were analyzed before and after freezing for motility, viability, plasma membrane integrity, and morphology. Furthermore, twenty mares were inseminated using post-thawed semen. Results: There were no differences observed among all extenders in all the parameters before freezing. Sperm cryopreserved using HF-20 showed better motility, viability, and plasma membrane integrity than Tris extender. The Tris extender showed the most inferior quality of post-thawed semen between all the extenders. HF-20, INRA Freeze®, and EquiPlus Freeze® extenders revealed the same capacity of semen preservation in vitro and in vivo. Conclusions: HF-20 extender has the same quality as INRA Freeze® and EquiPlus Freeze® that can be considered as one of the best extenders for the semen cryopreservation in horses. In contrast, Tris extender needs some degree of improvement.
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Publication Date: 2019-01-07 PubMed ID: 30936651PubMed Central: PMC6431823DOI: 10.14202/vetworld.2019.34-40Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the effectiveness of four different extenders (substances that aid in sperm preservation during freezing) on the quality of frozen horse semen. The best results were shown with the locally produced HF-20 extender, which performed as well as commercial extenders INRA Freeze and EquiPlus Freeze, while the Tris extender lagged behind.

Study Approach and Methodology

  • The researchers conducted this study to examine the impact of four different extenders on the quality of frozen semen in Arabian stallions. The selected extenders were HF-20 and Tris – locally produced extenders, alongside two commercial extenders: INRA Freeze (from IMV Technologies, France) and EquiPlus Freeze (from Minitube, Germany).
  • They collected a total of 15 ejaculates from three healthy stallions, all of which were cryopreserved in a consistent environment. Each collected semen sample was split into four equal parts and processed separately using each of the four extenders. This facilitated the comparison of the impact of each extender on the semen quality.
  • The team analyzed all samples before and after freezing. This allowed them to evaluate the extenders’ effectiveness at preserving sperm quality during the freezing process. The variables they observed included motility (the sperm’s ability to move), viability (the sperm’s capacity to live and grow), plasma membrane integrity (the outer shell health of the sperm), and morphology (the sperm’s shape and structure).

Research Findings and Results

  • Before the freezing procedure, no differences among all extenders in all the parameters were noted. This confirms that the ejaculates didn’t differ in quality prior to the application of extenders.
  • Post-thawed semen analysis showed differences among the extenders. The use of HF-20 extender resulted in better sperm motility, improved viability, and enhanced plasma membrane integrity as compared to the Tris extender.
  • Among all the extenders tested, the Tris extender’s results were the most inferior in preserving the quality of post-thawed semen.
  • On a comparative analysis, the HF-20, INRA Freeze, and EquiPlus Freeze extenders showed similar efficacy in preserving semen quality post-thaw.

Conclusions

  • According to the obtained results, the researchers concluded that the HF-20 extender proved to be of the same quality as the INRA Freeze and EquiPlus Freeze. This suggests that it could be considered as one of the best extenders for semen cryopreservation in horses.
  • In contrast, they suggested that the Tris extender needs some improvement due to its comparatively inferior performance in preserving post-thawed semen quality.

Cite This Article

APA
Alamaary MS, Haron AW, Ali M, Hiew MWH, Adamu L, Peter ID. (2019). Effects of four extenders on the quality of frozen semen in Arabian stallions. Vet World, 12(1), 34-40. https://doi.org/10.14202/vetworld.2019.34-40

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 12
Issue: 1
Pages: 34-40

Researcher Affiliations

Alamaary, Mohaammed Saad
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
  • King Abdulaziz Arabian Horses Center, Ministry of Agriculture, Riyadh, Saudi Arabia.
Haron, Abd Wahid
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
Ali, Mohamed
  • Department of Animal Production and Breeding, Faculty of Agriculture and Veterinary Medicine, AL Qassim University, AL Qassim State, Saudi Arabia.
Hiew, Mark Wen Han
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
Adamu, Lawan
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
  • Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria.
Peter, Innocent Damudu
  • Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
  • Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria.

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Citations

This article has been cited 9 times.
  1. Ullah A, Chen W, Shi L, Wang M, Geng M, Na J, Akhtar MF, Khan MZ, Wang C. Challenges and Enhancing Strategies of Equine Semen Preservation: Nutritional and Genetic Perspectives. Vet Sci 2025 Aug 25;12(9).
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  3. Zhang L, Wang X, Jiang C, Sohail T, Sun Y, Sun X, Wang J, Li Y. Effects of Different Diluents and Freezing Methods on Cryopreservation of Hu Ram Semen. Vet Sci 2024 Jun 3;11(6).
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  8. Wurlina W, Hariadi M, Safitri E, Susilowati S, Meles DK. The effect of crude guava leaf tannins on motility, viability, and intact plasma membrane of stored spermatozoa of Etawa crossbred goats. Vet World 2020 Mar;13(3):530-537.
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