Effects of hay intake and feeding sequence on variables in faeces and faecal water (dry matter, pH value, organic acids, ammonia, buffering capacity) of horses.
Abstract: To investigate effects of hay intake and feeding sequence on indicators of the microbial activity within the hindgut, six horses were fed 1.00 kg oats plus 0.50, 0.67, 0.83 or 1.00 kg hay/100 kg body weight (BW) x day, each for 14 days. Oats was offered either 30 min prior to hay (OA) or in the reversed sequence (HA) in a 2 x 8-week crossover design. Because typically exercised horses should be subjected to the study, faeces was used as substrate. Faecal dry matter (DM), the faecal waters' short-chain fatty acids (SCFA, in mmol/1) and molar percentages (mol%) of propionate and iso-butyrate were highest with OA (p < 0.01). Acetate mol%, acetate-propionate quotient (A/P) and buffering capacities 1 and 2 (BC1: current pH to pH 6; BC2: pH 6 to 5) of the faecal water were highest with HA (p < 0.01). While the hay intake rose, faecal pH, acetate mol%, A/P, BC1 and BC2 (the latter with HA only) increased (p < 0.05), but DM, SCFA and propionate mol% declined (p < 0.05). The hay-induced rise in A/P and BC1 was much higher with HA than with OA. L-Lactate and ammonia were unaffected by the feeding sequence and hay intake. In conclusion, hay intake and feeding sequence influence the microbial activity within the hindgut, although the concentrate level remains consistent. In horse rations with 1.00 kg oats/100 kg BW x day amounts of at least 0.83 kg hay/100 kg BW x day and offering the hay first seem to have the potency to protect the hindgut content from acidification. Behavioural abnormality was not observed any longer first with 1.00 kg hay/100 kg BW x day.
Publication Date: 2004-02-01 PubMed ID: 19774758DOI: 10.1111/j.1439-0396.2004.00447.xGoogle Scholar: Lookup
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- Clinical Trial
- Journal Article
Summary
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This research demonstrates that both the quantity of hay consumed by a horse and the sequence in which the horse is fed can influence the microbial activity in its hindgut, which in turn can affect the acidity of the horse’s fecal matter. Specifically, feeding a horse at least 0.83 kg of hay per 100 kg of bodyweight each day, before feeding it oats, can help protect the hindgut from becoming overly acidic.
Research Objective and Methodology
- The purpose of this research was to study the effects of the quantity of hay ingested and the sequence of feeding on indicators of microbial activity within a horse’s hindgut.
- Six horses were fed varying amounts of oats and hay on a daily basis for 14 days. The ratio was 1.00 kg oats plus between 0.50 kg to 1.00 kg of hay per 100 kg of bodyweight.
- The oats were either fed 30 minutes prior to the hay or the sequence was reversed.
Findings
- Faecal dry matter, short-chain fatty acids (SCFA) and proportions of propionate and iso-butyrate were highest when oats were fed first.
- The buffering capacities, acetate proportions and a measure called the acetate-propionate quotient (A/P) were highest when hay was fed first.
- As the hay intake increased, there were rises in faecal pH, acetate proportions, A/P, and buffering capacities, but declines in dry matter, SCFA, and propionate proportions.
- The rise in A/P and buffering capacity induced by hay was much higher when hay was fed before oats.
- Levels of L-lactate and ammonia remained unaffected by the feeding sequence and hay intake.
Implications and Conclusions
- The research concludes that both hay intake and feeding sequence can influence the microbial activity within the horse’s hindgut, even when the concentrate level remains the same.
- It is suggested that in horse diets consisting of 1.00 kg oats per 100 kg bodyweight per day, amounts of at least 0.83 kg hay per 100 kg bodyweight per day should be offered first to protect the hindgut from acidification.
- With such a feeding sequence and hay quantity, behavioural abnormalities, not specified in this summary, were no longer observed.
Cite This Article
APA
Zeyner A, Geissler C, Dittrich A.
(2004).
Effects of hay intake and feeding sequence on variables in faeces and faecal water (dry matter, pH value, organic acids, ammonia, buffering capacity) of horses.
J Anim Physiol Anim Nutr (Berl), 88(1-2), 7-19.
https://doi.org/10.1111/j.1439-0396.2004.00447.x Publication
Researcher Affiliations
- Institute of Animal Nutrition, Nutritional Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany. zeyner@vetmed.uni-leipzig.de
MeSH Terms
- Acids
- Ammonia / analysis
- Animal Feed / analysis
- Animal Husbandry
- Animal Nutritional Physiological Phenomena
- Animals
- Body Weight
- Cross-Over Studies
- Diet
- Feces / chemistry
- Feeding Behavior / physiology
- Female
- Horses / physiology
- Hydrogen-Ion Concentration
- Male
- Poaceae
- Water / analysis
Citations
This article has been cited 6 times.- Muhonen S, Sadet-Bourgeteau S, Julliand V. Effects of Differences in Fibre Composition and Maturity of Forage-Based Diets on the Microbial Ecosystem and Its Activity in Equine Caecum and Colon Digesta and Faeces. Animals (Basel) 2021 Aug 8;11(8).
- Zeyner A, Romanowski K, Vernunft A, Harris P, Müller AM, Wolf C, Kienzle E. Effects of Different Oral Doses of Sodium Chloride on the Basal Acid-Base and Mineral Status of Exercising Horses Fed Low Amounts of Hay. PLoS One 2017;12(1):e0168325.
- Franzan BC, da Silva Coelho I, Ramos EM, de Souza ARP, de Almeida FQ, Silva VP. Complete Extruded Diet: How Does Equine Fecal Microbiota Change During Intake Adaptation?. Anim Sci J 2026 Jan-Dec;97(1):e70147.
- Brandi LA, Nunes AT, Faleiros CA, Poleti MD, Oliveira ECM, Schmidt NT, Sousa RLM, Fukumasu H, Balieiro JCC, Brandi RA. Dietary Energy Sources Affect Cecal and Fecal Microbiota of Healthy Horses. Animals (Basel) 2024 Dec 3;14(23).
- Jensen RB, Walslag IH, Marcussen C, Thorringer NW, Junghans P, Nyquist NF. The effect of feeding order of forage and oats on metabolic and digestive responses related to gastric emptying in horses. J Anim Sci 2025 Jan 4;103.
- Yano R, Moriyama T, Fujimori M, Nishida T, Hanada M, Fukuma N. Effects of concentrate levels on intestinal fermentation and the microbial profile in Japanese draft horses. J Equine Sci 2023 Dec;34(4):101-109.
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