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Home / Equine Research Bank / Vet Sci / Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induce...
Veterinary sciences2020; 7(2); 57; doi: 10.3390/vetsci7020057

Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses-Clinical and Laboratorial Aspects.

Abstract: Several studies in human and equine medicine have produced controversial results regarding the role of dimethylsulfoxide (DMSO) as a therapeutic agent. This study aimed to evaluate the effect of joint lavage with different DMSO concentrations on biomarkers of synovial fluid inflammation and cartilage degradation in joints with lipopolysaccharide (LPS)-induced synovitis. Twenty-six tibiotarsal joints of 13 horses were randomly distributed into four groups (lactated Ringer's solution; 5% DMSO in lactated Ringer's; 10% DMSO in lactated Ringer's; and sham). All animals were evaluated for the presence of lameness, and synovial fluid analyses were performed at 0 h, 1 h, 8 h, 24 h, and 48 h (T0, T1, T8, T24, and T48, respectively). The white blood cell counts (WBC), total protein (TP), urea, prostaglandin E2 (PGE2), interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor-α (TNF-α), hyaluronic acid (HA), and chondroitin sulfate (CS) concentrations were measured. The WBC counts and PGE2, IL-1β, IL-6, and TP concentrations increased in all groups at T8 compared to baseline values (<0.05). At T48, only the 5% DMSO and 10% DMSO groups showed a significant decrease in WBC counts (<0.05). Furthermore, the 10% DMSO group had lower concentrations of PGE2 and IL-1β at T48 than at T8 (<0.05) and presented lower IL-6 levels than the5% DMSO and lactated Ringer's groups at T24. All groups showed an increase in CS concentration after LPS-induced synovitis. Joint lavage with 10% DMSO in lactated Ringer´s has anti-inflammatory but not chondroprotective effects.
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Publication Date: 2020-04-30 PubMed ID: 32365982PubMed Central: PMC7356314DOI: 10.3390/vetsci7020057Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the impacts of using different concentrations of dimethylsulfoxide (DMSO) in joint lavage treatments on horses with synthetically stimulated inflammation. The study found that lavage with a 10% DMSO solution reduced inflammation but did not demonstrate any significant protective effect on joint cartilage.

Objective and Methodology

  • This study explores the role of DMSO, a chemical compound with potential therapeutic attributes, in treating synovitis in horses. This is inflammation of the synovial fluid in joints which can cause severe discomfort and impaired movement.
  • Researchers induced synovitis in the joints of horses using lipopolysaccharide (LPS) and then treated them with varying concentrations of DMSO in a joint lavage procedure.
  • The horses were divided into four groups, each receiving a different treatment: lactated Ringer’s solution, 5% DMSO in lactated Ringer’s, 10% DMSO in lactated Ringer’s, and a sham treatment.
  • Throughout the study, the horses were consistently assessed for inflammation and cartilage health with key indicators being measured at several intervals.

Findings

  • The results demonstrated that all treatments resulted in increased levels of inflammation indicators including white blood cell counts and protein concentrations at 8 hours post-treatment.
  • However, only the groups treated with DMSO solutions showed a significant decrease in white blood cells at 48 hours post-treatment, indicating a successful reduction of inflammation.
  • The 10% DMSO solution was further linked to lower levels of other inflammation indicators, namely prostaglandin E2 and interleukin-1β, at 48 hours post-treatment, and lower interleukin-6 levels than the 5% DMSO and lactated Ringer’s solution groups at 24 hours post-treatment.
  • All groups, regardless of the treatment, displayed an increase in chondroitin sulfate concentration, a common marker of cartilage degeneration.

Conclusion

  • The research concludes that joint lavage treatment using a 10% DMSO concentration effectively reduces inflammation in synovitis-stricken horse joints.
  • However, this study found no evidence of a chondroprotective effect, meaning that while inflammation could be reduced, damage to the joint cartilage was not prevented.

Cite This Article

APA
Sotelo EDP, Vendruscolo CP, Fülber J, Seidel SRT, Jaramillo FM, Agreste FR, Silva LCLCD, Baccarin RYA. (2020). Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses-Clinical and Laboratorial Aspects. Vet Sci, 7(2), 57. https://doi.org/10.3390/vetsci7020057

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 7
Issue: 2
PII: 57

Researcher Affiliations

Sotelo, Eric Danilo Pauls
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Vendruscolo, Cynthia Prado
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Fülber, Joice
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Seidel, Sarah Raphaela Torquato
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Jaramillo, Fernando Mosquera
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Agreste, Fernanda Rodrigues
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Silva, Luís Cláudio Lopes Correia da
  • Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.
Baccarin, Raquel Yvonne Arantes
  • Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.

Grant Funding

  • 001 / Coordenau00e7u00e3o de Aperfeiu00e7oamento de Pessoal de Nu00edvel Superior

Conflict of Interest Statement

The authors declare that they have no conflict of interest.

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Citations

This article has been cited 2 times.
  1. Baccarin RYA, Seidel SRT, Michelacci YM, Tokawa PKA, Oliveira TM. Osteoarthritis: a common disease that should be avoided in the athletic horse's life.. Anim Front 2022 Jun;12(3):25-36.
    doi: 10.1093/af/vfac026pubmed: 35711506google scholar: lookup
  2. Watkins A, Fasanello D, Stefanovski D, Schurer S, Caracappa K, D'Agostino A, Costello E, Freer H, Rollins A, Read C, Su J, Colville M, Paszek M, Wagner B, Reesink H. Investigation of synovial fluid lubricants and inflammatory cytokines in the horse: a comparison of recombinant equine interleukin 1 beta-induced synovitis and joint lavage models.. BMC Vet Res 2021 May 12;17(1):189.
    doi: 10.1186/s12917-021-02873-2pubmed: 33980227google scholar: lookup
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