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Home / Equine Research Bank / Acta Cir Bras / Effects of platelet-rich plasma gel on skin healing in surgi...
Acta cirurgica brasileira2009; 24(4); 276-281; doi: 10.1590/s0102-86502009000400006

Effects of platelet-rich plasma gel on skin healing in surgical wound in horses.

Abstract: To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine. Methods: To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair. Results: The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation. Conclusions: Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine.
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Publication Date: 2009-08-26 PubMed ID: 19705027DOI: 10.1590/s0102-86502009000400006Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates a cost-effective method for creating a platelet-rich plasma (PRP) gel and examines its impact on surgical wound healing in horses.

Research Methodology

The researchers employed methods to produce a PRP gel, which they later used to evaluate wound healing effectiveness. Specific procedures involved:

  • Adding calcium gluconate and autologous thrombin to platelet-rich plasma in order to create a PRP gel.
  • Using six saddle horses for testing, making two surgical cuts on each animal.
  • Performing tests on wounds treated with PRP gel and untreated wounds for comparison.
  • Collecting wound biopsy samples at two treatment intervals: days 5 and 30 (Treatment 1), and days 15 and 45 post-wounding (Treatment 2).

Results

The results of the study showed that optimal platelet enrichment was achieved by two stages of centrifugation: first at 300 g for 10 minutes, and then at 640 g for 10 minutes. This provides valuable information for the preparation of the PRP gel as it illustrates the centrifugation process necessary for optimal platelet concentration.

Conclusion

The research concluded that wounds treated with PRP gel in horses showed improved healing compared to untreated wounds. The improvements were seen in two main areas:

  • Increased speed in epithelial differentiation. Epithelialization, or the process of forming a new surface layer on the wound to prevent infection and fluid loss, is a key part of wound healing.
  • Enhanced organization of dermal collagen. Collagen, the main structural protein in connective tissues, was better organized in wounds treated with PRP. Proper collagen formation and organization is crucial in wound healing as it lends strength and structure to the healing wound.

In conclusion, the study demonstrates that the PRP gel, a cost-effective wound treatment option in horses, can enhance the healing process when compared to untreated wounds.

Cite This Article

APA
DeRossi R, Coelho AC, Mello GS, Frazílio FO, Leal CR, Facco GG, Brum KB. (2009). Effects of platelet-rich plasma gel on skin healing in surgical wound in horses. Acta Cir Bras, 24(4), 276-281. https://doi.org/10.1590/s0102-86502009000400006

Publication

Acta cirurgica brasileira
ISSN: 1678-2674
NlmUniqueID: 9103983
Country: Brazil
Language: English
Volume: 24
Issue: 4
Pages: 276-281

Researcher Affiliations

DeRossi, Rafael
  • Department of Veterinary Medicine, Surgery and Anesthesiology, Faculty of Veterinary Medicine and Animal Science, UFMS, Mato Grosso do Sul, Brazil. derossi@nin.ufms.br
Coelho, Anna Carolina Anciliero de Oliveira
    Mello, Gisele Silveira de
      Frazílio, Fabrício Oliveira
        Leal, Cássia Rejane Brito
          Facco, Gilberto Gonçalves
            Brum, Karine Bonucielli

              MeSH Terms

              • Animals
              • Biopsy
              • Calcium Gluconate / therapeutic use
              • Dermatologic Surgical Procedures
              • Disease Models, Animal
              • Female
              • Gels
              • Hemostatics / therapeutic use
              • Horses
              • Male
              • Platelet-Rich Plasma
              • Skin / pathology
              • Skin / physiopathology
              • Thrombin / therapeutic use
              • Wound Healing / drug effects
              • Wound Healing / physiology
              • Wounds and Injuries / drug therapy
              • Wounds and Injuries / pathology
              • Wounds and Injuries / physiopathology

              Citations

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