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Home / Equine Research Bank / Am J Vet Res / Effects of platelet-rich plasma on the repair of wounds on t...
American journal of veterinary research2009; 70(2); 277-282; doi: 10.2460/ajvr.70.2.277

Effects of platelet-rich plasma on the repair of wounds on the distal aspect of the forelimb in horses.

Abstract: To evaluate the effect of platelet-rich plasma on wounds on the distal aspect of the forelimb in horses. Methods: 6 mixed-breed 10- to 15-year-old mares. Methods: 3 wounds were created on metacarpal regions in each of 6 horses (n = 36 wounds total). Eighteen wounds were treated with platelet-rich plasma and bandaged, whereas 18 control wounds were similarly bandaged with no prior topical treatment. Decrease in wound surface area and the required number of excisions of exuberant granulation tissue were recorded until complete healing. Tissue specimens were taken from wounds at 1 week for histologic examination and measurement of transforming growth factor-beta1 concentrations and at closure for histologic examination, biomechanical evaluation, and measurement of collagen type I and type III mRNA. Results: Platelet-rich plasma favored excessive development of granulation tissue and significantly slowed wound healing at 1, 2, and 3 weeks after surgery. Transforming growth factor-beta1 had a 1.6-fold higher concentration in treated wounds, compared with untreated wounds. Histologic, biomechanical, and gene expression data did not differ significantly between treated and control wounds. Conclusions: Topical application of autologous platelet-rich plasma did not accelerate or improve the quality of repair of small granulating wounds on limbs of horses. This treatment may better suit wounds with massive tissue loss or, alternatively, chronic wounds that would benefit from a fresh source of mediators to accelerate the healing process.
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Publication Date: 2009-02-24 PubMed ID: 19231962DOI: 10.2460/ajvr.70.2.277Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper studies the impact of using platelet-rich plasma (PRP) on healing wounds on horse limbs. It concludes that PRP didn’t significantly speed up or enhance the quality of small granulating wounds healing, but suggests it may be more effective for larger or chronic wounds.

Methods

  • The researchers conducted an experiment on six mixed-breed mares aged between 10 to 15 years.
  • Three wounds were deliberatley made on the metacarpal regions of each horse, amounting to a total of 36 wounds.
  • Half of these wounds (18) were treated with PRP and then bandaged, while the remaining 18 wounds were bandaged without receiving any topical treatment, serving as a control group.
  • The experiment monitored changes in wound surface area and took note of necessary exuberant granulation tissue excisions until the wounds were completely healed.
  • Tissue samples were collected from the wounds after one week for histological examination and measurement of transforming growth factor-beta1 (an essential protein involved in healing), and again at closure for further histological evaluation, biomechanical analysis, and measurement of collagen type I and type III mRNA (key components of wound healing).

Results

  • The research found that PRP encouraged the excessive development of granulation tissue which significantly slowed wound healing at 1, 2, and 3 weeks after surgery.
  • The concentration of transforming growth factor-beta1 was found to be 1.6 times higher in wounds treated with PRP compared to untreated ones, hinting at the role PRP could play in modulating healing.
  • No significant differences were observed in histologic, biomechanical, and gene expression data between PRP-treated and untreated wounds.

Conclusions

  • The use of autologous PRP (PRP procured from the patient’s own blood) did not speed up or improve the quality of repairing small wounds on the limbs of the horses.
  • The researchers suggest that this treatment could potentially be more effective for wounds involving substantial tissue loss or perhaps chronic wounds that could benefit from a supply of fresh healing mediators.

Cite This Article

APA
Monteiro SO, Lepage OM, Theoret CL. (2009). Effects of platelet-rich plasma on the repair of wounds on the distal aspect of the forelimb in horses. Am J Vet Res, 70(2), 277-282. https://doi.org/10.2460/ajvr.70.2.277

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 70
Issue: 2
Pages: 277-282

Researcher Affiliations

Monteiro, Susana O
  • Ecole Nationale Vétérinaire de Lyon, Département Hippique, Marcy L'Etoile, F-69280, France.
Lepage, Olivier M
    Theoret, Christine L

      MeSH Terms

      • Animals
      • Biomechanical Phenomena
      • Biopsy / veterinary
      • Collagen Type I / metabolism
      • Collagen Type III / metabolism
      • Forelimb / injuries
      • Horses / injuries
      • Platelet-Rich Plasma / physiology
      • RNA, Messenger / metabolism
      • Transforming Growth Factor beta / analysis
      • Wound Healing / drug effects
      • Wound Healing / physiology

      Citations

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