FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
American journal of veterinary research2009; 70(2); 277-282; doi: 10.2460/ajvr.70.2.277

Effects of platelet-rich plasma on the repair of wounds on the distal aspect of the forelimb in horses.

Abstract: To evaluate the effect of platelet-rich plasma on wounds on the distal aspect of the forelimb in horses. Methods: 6 mixed-breed 10- to 15-year-old mares. Methods: 3 wounds were created on metacarpal regions in each of 6 horses (n = 36 wounds total). Eighteen wounds were treated with platelet-rich plasma and bandaged, whereas 18 control wounds were similarly bandaged with no prior topical treatment. Decrease in wound surface area and the required number of excisions of exuberant granulation tissue were recorded until complete healing. Tissue specimens were taken from wounds at 1 week for histologic examination and measurement of transforming growth factor-beta1 concentrations and at closure for histologic examination, biomechanical evaluation, and measurement of collagen type I and type III mRNA. Results: Platelet-rich plasma favored excessive development of granulation tissue and significantly slowed wound healing at 1, 2, and 3 weeks after surgery. Transforming growth factor-beta1 had a 1.6-fold higher concentration in treated wounds, compared with untreated wounds. Histologic, biomechanical, and gene expression data did not differ significantly between treated and control wounds. Conclusions: Topical application of autologous platelet-rich plasma did not accelerate or improve the quality of repair of small granulating wounds on limbs of horses. This treatment may better suit wounds with massive tissue loss or, alternatively, chronic wounds that would benefit from a fresh source of mediators to accelerate the healing process.
Get Full Text
Publication Date: 2009-02-24 PubMed ID: 19231962DOI: 10.2460/ajvr.70.2.277Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper studies the impact of using platelet-rich plasma (PRP) on healing wounds on horse limbs. It concludes that PRP didn’t significantly speed up or enhance the quality of small granulating wounds healing, but suggests it may be more effective for larger or chronic wounds.

Methods

  • The researchers conducted an experiment on six mixed-breed mares aged between 10 to 15 years.
  • Three wounds were deliberatley made on the metacarpal regions of each horse, amounting to a total of 36 wounds.
  • Half of these wounds (18) were treated with PRP and then bandaged, while the remaining 18 wounds were bandaged without receiving any topical treatment, serving as a control group.
  • The experiment monitored changes in wound surface area and took note of necessary exuberant granulation tissue excisions until the wounds were completely healed.
  • Tissue samples were collected from the wounds after one week for histological examination and measurement of transforming growth factor-beta1 (an essential protein involved in healing), and again at closure for further histological evaluation, biomechanical analysis, and measurement of collagen type I and type III mRNA (key components of wound healing).

Results

  • The research found that PRP encouraged the excessive development of granulation tissue which significantly slowed wound healing at 1, 2, and 3 weeks after surgery.
  • The concentration of transforming growth factor-beta1 was found to be 1.6 times higher in wounds treated with PRP compared to untreated ones, hinting at the role PRP could play in modulating healing.
  • No significant differences were observed in histologic, biomechanical, and gene expression data between PRP-treated and untreated wounds.

Conclusions

  • The use of autologous PRP (PRP procured from the patient’s own blood) did not speed up or improve the quality of repairing small wounds on the limbs of the horses.
  • The researchers suggest that this treatment could potentially be more effective for wounds involving substantial tissue loss or perhaps chronic wounds that could benefit from a supply of fresh healing mediators.

Cite This Article

APA
Monteiro SO, Lepage OM, Theoret CL. (2009). Effects of platelet-rich plasma on the repair of wounds on the distal aspect of the forelimb in horses. Am J Vet Res, 70(2), 277-282. https://doi.org/10.2460/ajvr.70.2.277

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 70
Issue: 2
Pages: 277-282

Researcher Affiliations

Monteiro, Susana O
  • Ecole Nationale Vétérinaire de Lyon, Département Hippique, Marcy L'Etoile, F-69280, France.
Lepage, Olivier M
    Theoret, Christine L

      MeSH Terms

      • Animals
      • Biomechanical Phenomena
      • Biopsy / veterinary
      • Collagen Type I / metabolism
      • Collagen Type III / metabolism
      • Forelimb / injuries
      • Horses / injuries
      • Platelet-Rich Plasma / physiology
      • RNA, Messenger / metabolism
      • Transforming Growth Factor beta / analysis
      • Wound Healing / drug effects
      • Wound Healing / physiology

      Citations

      This article has been cited 22 times.
      1. Egli P, Boone L, Huber L, Higgins C, Gaonkar PP, Arrington J, Naskou MC, Peroni J, Gordon J, Lascola KM. Pilot study characterizing a single pooled preparation of equine platelet lysate for nebulization in the horse. Front Vet Sci 2024;11:1488942.
        doi: 10.3389/fvets.2024.1488942pubmed: 39726585google scholar: lookup
      2. Ribeiro G, Carvalho L, Borges J, Prazeres J. The Best Protocol to Treat Equine Skin Wounds by Second Intention Healing: A Scoping Review of the Literature. Animals (Basel) 2024 May 18;14(10).
        doi: 10.3390/ani14101500pubmed: 38791717google scholar: lookup
      3. Charlotte C P, Benoit B, Olivier M L. The effects of a synthetic epidermis spray on secondary intention wound healing in adult horses. PLoS One 2024;19(3):e0299990.
        doi: 10.1371/journal.pone.0299990pubmed: 38451976google scholar: lookup
      4. Chi L, Wang Q, Zhong W, Pan X, Li Y. The usefulness of platelet-rich plasma to manage skin wounds: A meta-analysis. Int Wound J 2023 Oct;20(8):3123-3130.
        doi: 10.1111/iwj.14188pubmed: 37128184google scholar: lookup
      5. Troha K, Vozel D, Arko M, Bedina Zavec A, Dolinar D, Hočevar M, Jan Z, Kisovec M, Kocjančič B, Pađen L, Pajnič M, Penič S, Romolo A, Repar N, Spasovski V, Steiner N, Šuštar V, Iglič A, Drobne D, Kogej K, Battelino S, Kralj-Iglič V. Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review. Int J Mol Sci 2023 Feb 8;24(4).
        doi: 10.3390/ijms24043420pubmed: 36834843google scholar: lookup
      6. Caruso M, Shuttle S, Amelse L, Elkhenany H, Schumacher J, Dhar MS. A pilot study to demonstrate the paracrine effect of equine, adult allogenic mesenchymal stem cells in vitro, with a potential for healing of experimentally-created, equine thoracic wounds in vivo. Front Vet Sci 2022;9:1011905.
        doi: 10.3389/fvets.2022.1011905pubmed: 36452146google scholar: lookup
      7. Mund SJK, MacPhee DJ, Campbell J, Honaramooz A, Wobeser B, Barber SM. Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses. Cells 2021 Nov 1;10(11).
        doi: 10.3390/cells10112972pubmed: 34831196google scholar: lookup
      8. Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front Vet Sci 2021;8:641818.
        doi: 10.3389/fvets.2021.641818pubmed: 33869321google scholar: lookup
      9. Iacopetti I, Patruno M, Melotti L, Martinello T, Bedin S, Badon T, Righetto EM, Perazzi A. Autologous Platelet-Rich Plasma Enhances the Healing of Large Cutaneous Wounds in Dogs. Front Vet Sci 2020;7:575449.
        doi: 10.3389/fvets.2020.575449pubmed: 33195571google scholar: lookup
      10. Tsai HC, Chang GR, Fan HC, Ou-Yang H, Huang LC, Wu SC, Chen CM. A mini-pig model for evaluating the efficacy of autologous platelet patches on induced acute full thickness wound healing. BMC Vet Res 2019 Jun 7;15(1):191.
        doi: 10.1186/s12917-019-1932-7pubmed: 31174527google scholar: lookup
      11. Sills ES, Wood SH. Autologous activated platelet-rich plasma injection into adult human ovary tissue: molecular mechanism, analysis, and discussion of reproductive response. Biosci Rep 2019 Jun 28;39(6).
        doi: 10.1042/BSR20190805pubmed: 31092698google scholar: lookup
      12. Tambella AM, Attili AR, Dupré G, Cantalamessa A, Martin S, Cuteri V, Marcazzan S, Del Fabbro M. Platelet-rich plasma to treat experimentally-induced skin wounds in animals: A systematic review and meta-analysis. PLoS One 2018;13(1):e0191093.
        doi: 10.1371/journal.pone.0191093pubmed: 29324848google scholar: lookup
      13. Shin HS, Woo HM, Kang BJ. Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma. BMC Vet Res 2017 Jun 26;13(1):198.
        doi: 10.1186/s12917-017-1123-3pubmed: 28651609google scholar: lookup
      14. Jee CH, Eom NY, Jang HM, Jung HW, Choi ES, Won JH, Hong IH, Kang BT, Jeong DW, Jung DI. Effect of autologous platelet-rich plasma application on cutaneous wound healing in dogs. J Vet Sci 2016 Mar;17(1):79-87.
        doi: 10.4142/jvs.2016.17.1.79pubmed: 27051343google scholar: lookup
      15. João De Masi EC, Campos AC, João De Masi FD, Ratti MA, Ike IS, João De Masi RD. The influence of growth factors on skin wound healing in rats. Braz J Otorhinolaryngol 2016 Sep-Oct;82(5):512-21.
        doi: 10.1016/j.bjorl.2015.09.011pubmed: 26832633google scholar: lookup
      16. de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG. Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma. Biomed Res Int 2015;2015:893485.
        doi: 10.1155/2015/893485pubmed: 26236743google scholar: lookup
      17. Tracey AK, Alcott CJ, Schleining JA, Safayi S, Zaback PC, Hostetter JM, Reinertson EL. The effects of topical oxygen therapy on equine distal limb dermal wound healing. Can Vet J 2014 Dec;55(12):1146-52.
        pubmed: 25477541
      18. Perazzi A, Busetto R, Martinello T, Drigo M, Pasotto D, Cian F, Patruno M, Iacopetti I. Description of a double centrifugation tube method for concentrating canine platelets. BMC Vet Res 2013 Jul 22;9:146.
        doi: 10.1186/1746-6148-9-146pubmed: 23876182google scholar: lookup
      19. Fontenot RL, Sink CA, Werre SR, Weinstein NM, Dahlgren LA. Simple tube centrifugation for processing platelet-rich plasma in the horse. Can Vet J 2012 Dec;53(12):1266-72.
        pubmed: 23729823
      20. Silva RF, Carmona JU, Rezende CM. Ultrastructural characteristics of fibrin clots from canine and feline platelet concentrates activated with calcium gluconate or calcium gluconate plus batroxobin. BMC Vet Res 2013 Apr 15;9:77.
        doi: 10.1186/1746-6148-9-77pubmed: 23587176google scholar: lookup
      21. Giraldo CE, López C, Álvarez ME, Samudio IJ, Prades M, Carmona JU. Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res 2013 Feb 12;9:29.
        doi: 10.1186/1746-6148-9-29pubmed: 23402541google scholar: lookup
      22. Silva RF, Alvarez ME, Ríos DL, López C, Carmona JU, Rezende CM. Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates. BMC Vet Res 2012 Nov 6;8:212.
        doi: 10.1186/1746-6148-8-212pubmed: 23131192google scholar: lookup
      Subscribe to our newsletter
      Join 99,727 horse owners like you who receive our email updates on horse health and nutrition.