Effects of preparation method, shear force, and exposure to collagen on release of growth factors from equine platelet-rich plasma.
Abstract: To test the hypotheses that preparation method, exposure to shear force, and exposure to collagen affect the release of growth factors from equine platelet-rich plasma (PRP). Methods: PRP obtained from 6 horses. Methods: PRP was prepared via 2 preparation methods (tube and automated) and subjected to 6 treatment conditions (resting, detergent, exposure to shear via 21- and 25-gauge needles, and exposure to collagen [10 and 20 μg/mL]). Concentrations of platelet-derived growth factor, isoform BB (PDGF-BB); transforming growth factor β, isoform 1 (TGFβ₁); and insulin-like growth factor, isoform 1 (IGF-1) were quantified by use of ELISAs. Statistical analysis was conducted via repeated-measures ANOVA. Results: Platelet numbers were significantly higher in tube-prepared PRP than in automated-prepared PRP Growth factor concentrations did not differ significantly between preparation methods. Mean PDGF-BB concentration ranged from 134 to 7,157 pg/mL, mean TGFβ₁ concentration ranged from 1,153 to 22,677 pg/mL, and mean IGF-1 concentration ranged from 150 to 280 ng/mL. Shear force did not affect growth factor concentrations. Dose-dependent increases in PDGF-BB and TGFβ₁ were detected in response to collagen, but equalled only 10% of the estimated total platelet content. Concentrations of IGF-1 were not significantly different among treatments and negative or positive control treatments. Serum concentrations of PDGF-BB and TGFβ₁ exceeded concentrations in PRP for most treatment conditions. Conclusions: Release of growth factors from equine PRP was negligible as a result of the injection process alone. Investigation of platelet-activation protocols is warranted to potentially enhance PRP treatment efficacy in horses.
Publication Date: 2011-02-02 PubMed ID: 21281204DOI: 10.2460/ajvr.72.2.271Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper investigates how the preparation method, exposure to shear force, and exposure to collagen can affect the release of growth factors from horse Platelet-Rich Plasma (PRP). The study found that while preparation methods have no significant impact on growth factor concentrations, collagen exposure does result in dose-dependent increases for two growth factors.
Sample and Preparation
- The researchers obtained PRP from six horses. This was done using two different preparation methods known as the tube and automated methods.
Test Conditions
- The PRP was subjected to six treatment conditions including rest, detergent application, exposure to shear force via two different gauge needles, and exposure to two concentrations of collagen.
Growth Factor Analysis
- The research evaluated the concentration of three key growth factors: platelet-derived growth-factor, isoform BB (PDGF-BB); transforming growth factor-β, isoform 1 (TGFβ₁); and insulin-like growth factor, isoform 1 (IGF-1).
- The concentrations of these growth factors were quantified using enzyme-linked immunosorbent assays (ELISAs).
Findings
- The findings showed a significantly higher number of platelets in PRP prepared via the tube method, as compared to the automated method.
- However, the research did not find any significant difference in the concentration of growth factors between the two preparation methods.
- Interestingly, shear force exposure had no noticeable effect on the concentration of growth factors within the PRP.
- Exposure to collagen did result in dose-dependent increases in PDGF-BB and TGFβ₁ growth factors, equaling about 10% of the total estimated platelet content.
- The concentrations of IGF-1 remained largely the same across all treatment conditions.
- The research team found that serum concentrations of PDGF-BB and TGFβ₁ exceeded concentrations in PRP for most treatment conditions.
Conclusions
- From the results, the researchers concluded that the release of growth factors from equine PRP due to the injection process itself was negligible.
- They suggested further investigation into platelet-activation protocols to potentially enhance PRP treatment efficacy in horses.
Cite This Article
APA
Textor JA, Norris JW, Tablin F.
(2011).
Effects of preparation method, shear force, and exposure to collagen on release of growth factors from equine platelet-rich plasma.
Am J Vet Res, 72(2), 271-278.
https://doi.org/10.2460/ajvr.72.2.271 Publication
Researcher Affiliations
- Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA. jamietextor@gmail.com
MeSH Terms
- Animals
- Collagen / chemistry
- Horses / blood
- Intercellular Signaling Peptides and Proteins / metabolism
- Platelet-Rich Plasma / physiology
Citations
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