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Home / Equine Research Bank / BMC Vet Res / Effects of the breed, sex and age on cellular content and gr...
BMC veterinary research2013; 9; 29; doi: 10.1186/1746-6148-9-29

Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.

Abstract: There is no information on the effects of the breed, gender and age on the cellular content and growth factor (GF) release from equine pure-platelet rich plasma (P-PRP) and pure-platelet rich gel (P-PRG). The objectives of this study were: 1) to compare the cellular composition of P-PRP with whole blood and platelet poor plasma (PPP); 2) to compare the concentration of transforming GF beta 1 (TGF-β1) and platelet derived GF isoform BB (PDGF-BB) between P-PRP treated with non-ionic detergent (P-PRP+NID), P-PRG (activated with calcium gluconate -CG-), PPP+NID, PPP gel (PPG), and plasma and; 3) to evaluate and to correlate the effect of the breed, gender and age on the cellular and GF concentration for each blood component. Forty adult horses, 20 Argentinean Creole Horses (ACH) and, 20 Colombian Creole Horses (CCH) were included. Data were analyzed by parametric (i.e.: t-test, one way ANOVA) and non parametric (Kruskal-Wallis test, Wilcoxon test) tests. Correlation analysis was also performed by using the Spearman and Pearson tests. A p ≤ 0.05 was set as significant for all tests. All the blood components were compared for platelet (PLT), leukocyte (WBC), TGF-β1 and PDGF-BB concentrations. The effect of the breed, gender and age on these variables was analyzed. A P ≤ 0.05 was accepted as significant for all the tests. Results: PLT counts were 1.8 and 0.6 times higher in P-PRP than in whole blood and PPP, respectively; WBC counts were 0.5 and 0.1 times lower in P-PRP, in comparison with whole blood and PPP, respectively. TGF-β1 and PDGF-BB concentrations were 2.3 and 262 times higher, respectively, in P-PRG than in plasma, and 0.59 and 0.48 times higher, respectively, in P-PRG than in PPG. P-PRG derived from CCH females or young horses presented significantly (P < 0.001) higher PDGF-BB concentrations than P-PRG derived from ACH males or older horses. Conclusions: Our results indicated that P-PRP obtained by a manual method was affected by intrinsic factors such as the breed, gender and age. Equine practitioners should be aware that cellular and GF release from P-PRP/P-PRG could change according with the intrinsic variables associated with a patient in particular.
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Publication Date: 2013-02-12 PubMed ID: 23402541PubMed Central: PMC3577464DOI: 10.1186/1746-6148-9-29Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research studies the impact of horse breed, sex, and age on the cell content and growth factor release from pure-platelet rich plasma and pure-platelet rich gel in horses. It found that these factors do influence the platelet and leukocyte counts, as well as growth factor concentrations, highlighting then need for tailored treatment strategies in equine medicine.

Research Objectives and Methodology

  • The primary aim of this study was to understand the influence of breed, gender, and age on the cellular composition, specifically platelet and leukocyte counts, and the release of growth factors (TGF-β1 and PDGF-BB) from platelet-rich plasma and gel in horses. The cellular composition of pure-platelet rich plasma (P-PRP) was compared with whole blood and platelet poor plasma (PPP).
  • The study involved 40 adult horses, including Argentinean Creole Horses and Colombian Creole Horses. The research conducted various statistical tests, including parametric tests like t-test and ANOVA, and non-parametric tests such as Kruskal-Wallis test and Wilcoxon test, to analyze the collected data. Correlation analysis was also done to draw relationships between the variables.

Results

  • The researchers found that platelet counts in P-PRP were 1.8 and 0.6 times higher compared to those in whole blood and PPP respectively. On the other hand, the leukocyte counts were comparatively lower in P-PRP than in whole blood and PPP.
  • Interestingly, the concentrations of growth factors, TGF-β1 and PDGF-BB, were found to be significantly higher in P-PRP and P-PRG compared to plasma and platelet-poor plasma gel (PPP gel). The concentrations of these growth factors were also higher in P-PRG derived from Colombian Creole Horse females and younger horses.

Conclusions

  • The research concludes that the method of obtaining P-PRP manually gets influenced by factors such as breed, gender, and age of the horse. This implies a notable impact of these factors on the cellular content and growth factor release from equine P-PRP and P-PRG.
  • This indicates that veterinarians and equine practitioners must consider these intrinsic variables when handling individual cases. It eventually points to the need for personalized treatment strategies in equine medicine for optimized outcomes.

Cite This Article

APA
Giraldo CE, López C, Álvarez ME, Samudio IJ, Prades M, Carmona JU. (2013). Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res, 9, 29. https://doi.org/10.1186/1746-6148-9-29

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 9
Pages: 29

Researcher Affiliations

Giraldo, Carlos E
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
López, Catalina
    Álvarez, María E
      Samudio, Ismael J
        Prades, Marta
          Carmona, Jorge U

            MeSH Terms

            • Age Factors
            • Animals
            • Blood Platelets / metabolism
            • Blood Platelets / physiology
            • Female
            • Horses / blood
            • Horses / physiology
            • Leukocyte Count / veterinary
            • Male
            • Platelet Count / veterinary
            • Platelet-Derived Growth Factor / metabolism
            • Platelet-Derived Growth Factor / physiology
            • Platelet-Rich Plasma / cytology
            • Platelet-Rich Plasma / metabolism
            • Platelet-Rich Plasma / physiology
            • Protein Isoforms
            • Sex Factors
            • Species Specificity
            • Transforming Growth Factor beta1 / metabolism
            • Transforming Growth Factor beta1 / physiology

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