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BMC veterinary research2013; 9; 29; doi: 10.1186/1746-6148-9-29

Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel.

Abstract: There is no information on the effects of the breed, gender and age on the cellular content and growth factor (GF) release from equine pure-platelet rich plasma (P-PRP) and pure-platelet rich gel (P-PRG). The objectives of this study were: 1) to compare the cellular composition of P-PRP with whole blood and platelet poor plasma (PPP); 2) to compare the concentration of transforming GF beta 1 (TGF-β1) and platelet derived GF isoform BB (PDGF-BB) between P-PRP treated with non-ionic detergent (P-PRP+NID), P-PRG (activated with calcium gluconate -CG-), PPP+NID, PPP gel (PPG), and plasma and; 3) to evaluate and to correlate the effect of the breed, gender and age on the cellular and GF concentration for each blood component. Forty adult horses, 20 Argentinean Creole Horses (ACH) and, 20 Colombian Creole Horses (CCH) were included. Data were analyzed by parametric (i.e.: t-test, one way ANOVA) and non parametric (Kruskal-Wallis test, Wilcoxon test) tests. Correlation analysis was also performed by using the Spearman and Pearson tests. A p ≤ 0.05 was set as significant for all tests. All the blood components were compared for platelet (PLT), leukocyte (WBC), TGF-β1 and PDGF-BB concentrations. The effect of the breed, gender and age on these variables was analyzed. A P ≤ 0.05 was accepted as significant for all the tests. Results: PLT counts were 1.8 and 0.6 times higher in P-PRP than in whole blood and PPP, respectively; WBC counts were 0.5 and 0.1 times lower in P-PRP, in comparison with whole blood and PPP, respectively. TGF-β1 and PDGF-BB concentrations were 2.3 and 262 times higher, respectively, in P-PRG than in plasma, and 0.59 and 0.48 times higher, respectively, in P-PRG than in PPG. P-PRG derived from CCH females or young horses presented significantly (P < 0.001) higher PDGF-BB concentrations than P-PRG derived from ACH males or older horses. Conclusions: Our results indicated that P-PRP obtained by a manual method was affected by intrinsic factors such as the breed, gender and age. Equine practitioners should be aware that cellular and GF release from P-PRP/P-PRG could change according with the intrinsic variables associated with a patient in particular.
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Publication Date: 2013-02-12 PubMed ID: 23402541PubMed Central: PMC3577464DOI: 10.1186/1746-6148-9-29Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research studies the impact of horse breed, sex, and age on the cell content and growth factor release from pure-platelet rich plasma and pure-platelet rich gel in horses. It found that these factors do influence the platelet and leukocyte counts, as well as growth factor concentrations, highlighting then need for tailored treatment strategies in equine medicine.

Research Objectives and Methodology

  • The primary aim of this study was to understand the influence of breed, gender, and age on the cellular composition, specifically platelet and leukocyte counts, and the release of growth factors (TGF-β1 and PDGF-BB) from platelet-rich plasma and gel in horses. The cellular composition of pure-platelet rich plasma (P-PRP) was compared with whole blood and platelet poor plasma (PPP).
  • The study involved 40 adult horses, including Argentinean Creole Horses and Colombian Creole Horses. The research conducted various statistical tests, including parametric tests like t-test and ANOVA, and non-parametric tests such as Kruskal-Wallis test and Wilcoxon test, to analyze the collected data. Correlation analysis was also done to draw relationships between the variables.

Results

  • The researchers found that platelet counts in P-PRP were 1.8 and 0.6 times higher compared to those in whole blood and PPP respectively. On the other hand, the leukocyte counts were comparatively lower in P-PRP than in whole blood and PPP.
  • Interestingly, the concentrations of growth factors, TGF-β1 and PDGF-BB, were found to be significantly higher in P-PRP and P-PRG compared to plasma and platelet-poor plasma gel (PPP gel). The concentrations of these growth factors were also higher in P-PRG derived from Colombian Creole Horse females and younger horses.

Conclusions

  • The research concludes that the method of obtaining P-PRP manually gets influenced by factors such as breed, gender, and age of the horse. This implies a notable impact of these factors on the cellular content and growth factor release from equine P-PRP and P-PRG.
  • This indicates that veterinarians and equine practitioners must consider these intrinsic variables when handling individual cases. It eventually points to the need for personalized treatment strategies in equine medicine for optimized outcomes.

Cite This Article

APA
Giraldo CE, López C, Álvarez ME, Samudio IJ, Prades M, Carmona JU. (2013). Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res, 9, 29. https://doi.org/10.1186/1746-6148-9-29

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 9
Pages: 29

Researcher Affiliations

Giraldo, Carlos E
  • Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, Colombia.
López, Catalina
    Álvarez, María E
      Samudio, Ismael J
        Prades, Marta
          Carmona, Jorge U

            MeSH Terms

            • Age Factors
            • Animals
            • Blood Platelets / metabolism
            • Blood Platelets / physiology
            • Female
            • Horses / blood
            • Horses / physiology
            • Leukocyte Count / veterinary
            • Male
            • Platelet Count / veterinary
            • Platelet-Derived Growth Factor / metabolism
            • Platelet-Derived Growth Factor / physiology
            • Platelet-Rich Plasma / cytology
            • Platelet-Rich Plasma / metabolism
            • Platelet-Rich Plasma / physiology
            • Protein Isoforms
            • Sex Factors
            • Species Specificity
            • Transforming Growth Factor beta1 / metabolism
            • Transforming Growth Factor beta1 / physiology

            References

            This article includes 23 references
            1. Carmona JU, Argüelles D, Climent F, Prades M. Autologous platelet concentrates as a treatment of horses with osteoarthritis: a preliminary pilot clinical study.. J Equine Vet Sci 2007;27(4):167–170.
              doi: 10.1016/j.jevs.2007.02.007google scholar: lookup
            2. Argüelles D, Carmona JU, Climent F, Munoz E, Prades M. Autologous platelet concentrates as a treatment for musculoskeletal lesions in five horses.. Vet Rec 2008;162(7):208–211.
              doi: 10.1136/vr.162.7.208pubmed: 18281627google scholar: lookup
            3. Castelijns G, Crawford A, Schaffer J, Ortolano GA, Beauregard T, Smith RKW. Evaluation of a filter-prepared platelet concentrate for the treatment of suspensory branch injuries in horses.. Vet Comp Orthop Traumatol 2011;24(5):363–369.
              doi: 10.3415/VCOT-11-01-0001pubmed: 21887455google scholar: lookup
            4. Waselau M, Sutter WW, Genovese RL, Bertone AL. Intralesional injection of platelet-rich plasma followed by controlled exercise for treatment of midbody suspensory ligament desmitis in Standardbred racehorses.. J Am Vet Med Assc 2008;232(10):1515–1520.
              doi: 10.2460/javma.232.10.1515pubmed: 18479242google scholar: lookup
            5. Monteiro SO, Lepage OM, Theoret CL. Effects of platelet-rich plasma on the repair of wounds on the distal aspect of the forelimb in horses.. Am J Vet Res 2009;70(2):277–282.
              doi: 10.2460/ajvr.70.2.277pubmed: 19231962google scholar: lookup
            6. Dohan Ehrenfest DM, Bielecki T, Mishra A, Borzini P, Inchingolo F, Sammartino G, Rasmusson L, Everts PA. In search of a consensus terminology in the field of platelet concentrates for surgical use: platelet-rich plasma (PRP), platelet-rich fibrin (PRF), fibrin gel polymerization and leukocytes.. Curr Pharm Biotechnol 2012;13:1131–1137.
              doi: 10.2174/138920112800624328pubmed: 21740379google scholar: lookup
            7. Carmona JU, López C, Sandoval JA. Review of the currently available systems to obtain platelet related products to treat equine musculoskeletal injuries.. Rec Pat Reg Med 2013;3:2.
              doi: 10.2174/2210212700122882965google scholar: lookup
            8. Argüelles D, Carmona JU, Pastor J, Iborra A, Viñals L, Martínez P, Bach E, Prades M. Evaluation of single and double centrifugation tube methods for concentrating equine platelets.. Res Vet Sci 2006;81(2):237–245.
              doi: 10.1016/j.rvsc.2005.12.008pubmed: 16969921google scholar: lookup
            9. Sutter WW, Kaneps AJ, Bertone AL. Comparison of hematologic values and transforming growth factor-β and isulinlike growth factor concentrations in platelets concentrates obtained by use of buffy coat and apheresis methods from equine blood.. Am J Vet Res 2004;65:924–930.
              doi: 10.2460/ajvr.2004.65.924pubmed: 15281650google scholar: lookup
            10. Textor JA, Norris JW, Tablin F. Effects of preparation method, shear force, and exposure to collagen on release of growth factors from equine platelet-rich plasma.. Am J Vet Res 2011;72(2):271–278.
              doi: 10.2460/ajvr.72.2.271pubmed: 21281204google scholar: lookup
            11. Fortier L, Barker J, Strauss E, McCarrel T, Cole B. The role of growth factors in cartilage repair.. Clin Orthop Relat Res 2011;469(10):2706–2715.
              doi: 10.1007/s11999-011-1857-3pmc: PMC3171543pubmed: 21403984google scholar: lookup
            12. Zimmermann R, Jakubietz R, Jakubietz M, Strasser E, Schlegel A, Wiltfang J, Eckstein R. Different preparation methods to obtain platelet components as a source of growth factors for local application.. Transfusion 2001;41(10):1217–1224.
              doi: 10.1046/j.1537-2995.2001.41101217.xpubmed: 11606819google scholar: lookup
            13. Vendruscolo CP, Carvalho AM, Moraes LF, Maia L, Queiroz DL, Watanabe MJ, Yamada ALM, Alves ALG. Avaliação da eficácia de diferentes protocolos de preparo do Plasma Rico em Plaquetas para uso em Medicina Equina.. Pesquisa Vet Brasil 2012;32(2):106–110.
              doi: 10.1590/S0100-736X2012000200002google scholar: lookup
            14. Schnabel LV, Mohammed HO, Jacobson MS, Fortier LA. Effects of platelet rich plasma and acellular bone marrow on gene expression patterns and DNA content of equine suspensory ligament explant cultures.. Equine Vet J 2008;40(3):260–265.
              doi: 10.2746/042516408X278030pubmed: 18267879google scholar: lookup
            15. Bosch G, van Schie HTA, de Groot MW, Cadby JA, van de Lest CHA, Barneveld A, van Weeren PR. Effects of platelet-rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: a placebo-controlled experimental study.. J Orthop Res 2010;28(2):211–217.
              pubmed: 19714688
            16. Carmona JU, López C. Questions statistical power of study on release of growth factors from platelet-rich plasma.. Am J Vet Res 2011;72(8):998–999.
              pubmed: 21801054
            17. Wilmink JM, van Weeren PR. Differences in wound healing between horses and ponies: Application of research results to the clinical approach of equine wounds.. Clin Tech Equine P 2004;3(2):123–133.
              doi: 10.1053/j.ctep.2004.08.011google scholar: lookup
            18. Nixon AJ, Brower-Toland BD, Sandell LJ. Primary nucleotide structure of predominant and alternate splice forms of equine insulin-like growth factor I and their gene expression patterns in tissues.. Am J Vet Res 1999;60(10):1234–1241.
              pubmed: 10791936
            19. Butkiewicz AM, Kemona H, Dymicka-Piekarska V, Matowicka-Karna J, Radziwon P, Lipska A. Platelet count, mean platelet volume and thrombocytopoietic indices in healthy women and men.. Thromb Res 2006;118(2):199–204.
              doi: 10.1016/j.thromres.2005.06.021pubmed: 16139337google scholar: lookup
            20. Oertelt-Prigione S. The influence of sex and gender on the immune response.. Autoimmun Rev 2012;11:A479–A485.
              doi: 10.1016/j.autrev.2011.11.022pubmed: 22155201google scholar: lookup
            21. Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction.. J BiolChem 1949;177(2):751–766.
              pubmed: 18110453
            22. Penha-goncalves MN, Onions DE, Nicolson L. Cloning and sequencing of equine transforming growth factor-beta 1 (TGF beta-1) cDNA.. DNA Seq 1997;7(6):375–378.
              pubmed: 9524819
            23. Donnelly BP, Nixon AJ, Haupt JL. Nucleotide structure of equine platelet-derived growth factor-A and –B and expression in horses with induced acute tendinitis.. Am J Vet Res 2006;67(7):1218–1225.
              doi: 10.2460/ajvr.67.7.1218pubmed: 16817746google scholar: lookup

            Citations

            This article has been cited 38 times.
            1. Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
              doi: 10.3390/ani15243586pubmed: 41463871google scholar: lookup
            2. Colomer-Selva R, Tvarijonavciute A, Franco-Martínez L, Hernández-Guerra ÁM, Carrillo JM, Rubio M, Sopena JJ, Satué K. Physiological factors affecting platelet-rich plasma variability in human and veterinary medicine. Front Vet Sci 2025;12:1571373.
              doi: 10.3389/fvets.2025.1571373pubmed: 40470272google scholar: lookup
            3. Vlasova TI, Brodovskaya EP, Madonov KS, Ageev VP, Abelova AP, Pinyaev SI, Vlasov AP. Regenerative Potential of Platelet-Rich Plasma in Diabetes Mellitus. Dokl Biol Sci 2025 Apr;521(1):123-133.
              doi: 10.1134/S0012496624600556pubmed: 40216681google scholar: lookup
            4. Liu R, Chen S, Wang P, Bi R. Using platelet concentrates to treat maxillofacial tissue lesions. Front Bioeng Biotechnol 2024;12:1523225.
              doi: 10.3389/fbioe.2024.1523225pubmed: 39845369google scholar: lookup
            5. Egli P, Boone L, Huber L, Higgins C, Gaonkar PP, Arrington J, Naskou MC, Peroni J, Gordon J, Lascola KM. Pilot study characterizing a single pooled preparation of equine platelet lysate for nebulization in the horse. Front Vet Sci 2024;11:1488942.
              doi: 10.3389/fvets.2024.1488942pubmed: 39726585google scholar: lookup
            6. Yaneselli K, Ávila G, Rossi A, Rial A, Castro S, Estradé MJ, Suárez G, Algorta A. Impact of different formulations of platelet lysate on proliferative and immune profile of equine mesenchymal stromal cells. Front Vet Sci 2024;11:1410855.
              doi: 10.3389/fvets.2024.1410855pubmed: 39161460google scholar: lookup
            7. Reis IL, Lopes B, Sousa P, Sousa AC, Caseiro AR, Mendonça CM, Santos JM, Atayde LM, Alvites RD, Maurício AC. Equine Musculoskeletal Pathologies: Clinical Approaches and Therapeutical Perspectives-A Review. Vet Sci 2024 Apr 26;11(5).
              doi: 10.3390/vetsci11050190pubmed: 38787162google scholar: lookup
            8. Torres LEC, Florez CO, Oliveira JG, Vieira GD, Ribeiro IS, Keller KM, Leme FOP, Fantini P, Maranhão RPA. Antimicrobial Activity of Plasma Rich in Platelets (PRP) on the Ocular Microbiota of Healthy Horses from Minas Gerais: In Vitro Study. Vet Med Int 2023;2023:2407768.
              doi: 10.1155/2023/2407768pubmed: 37622165google scholar: lookup
            9. Chi L, Wang Q, Zhong W, Pan X, Li Y. The usefulness of platelet-rich plasma to manage skin wounds: A meta-analysis. Int Wound J 2023 Oct;20(8):3123-3130.
              doi: 10.1111/iwj.14188pubmed: 37128184google scholar: lookup
            10. Frias-De-Diego A, Gilbertie JM, Scholle F, Dejarnette S, Crisci E. Effect of BIO-PLY(TM), a Platelet-Rich Plasma Derived Biologic on PRRSV-2-Infected Macrophages. Viruses 2022 Nov 28;14(12).
              doi: 10.3390/v14122666pubmed: 36560670google scholar: lookup
            11. Wu MJM, Sermer C, Kandel RA, Theodoropoulos JS. Characterization of Migratory Cells From Bioengineered Bovine Cartilage in a 3D Co-culture Model. Am J Sports Med 2022 Sep;50(11):3090-3101.
              doi: 10.1177/03635465221113325pubmed: 35983988google scholar: lookup
            12. Gilbertie JM, Schaer TP, Engiles JB, Seiler GS, Deddens BL, Schubert AG, Jacob ME, Stefanovski D, Ruthel G, Hickok NJ, Stowe DM, Frink A, Schnabel LV. A Platelet-Rich Plasma-Derived Biologic Clears Staphylococcus aureus Biofilms While Mitigating Cartilage Degeneration and Joint Inflammation in a Clinically Relevant Large Animal Infectious Arthritis Model. Front Cell Infect Microbiol 2022;12:895022.
              doi: 10.3389/fcimb.2022.895022pubmed: 35711655google scholar: lookup
            13. Hagen A, Holland H, Brandt VP, Doll CU, Häußler TC, Melzer M, Moellerberndt J, Lehmann H, Burk J. Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same. Animals (Basel) 2022 Jan 13;12(2).
              doi: 10.3390/ani12020189pubmed: 35049811google scholar: lookup
            14. Fantini P, Jiménez R, Vilés K, Iborra A, Palhares MS, Catalán J, Prades M, Miró J. Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis. Animals (Basel) 2021 Oct 9;11(10).
              doi: 10.3390/ani11102918pubmed: 34679937google scholar: lookup
            15. Camargo Garbin L, Lopez C, Carmona JU. A Critical Overview of the Use of Platelet-Rich Plasma in Equine Medicine Over the Last Decade. Front Vet Sci 2021;8:641818.
              doi: 10.3389/fvets.2021.641818pubmed: 33869321google scholar: lookup
            16. Montano C, Auletta L, Greco A, Costanza D, Coluccia P, Del Prete C, Meomartino L, Pasolini MP. The Use of Platelet-Rich Plasma for Treatment of Tenodesmic Lesions in Horses: A Systematic Review and Meta-Analysis of Clinical and Experimental Data. Animals (Basel) 2021 Mar 12;11(3).
              doi: 10.3390/ani11030793pubmed: 33809227google scholar: lookup
            17. Hagen A, Lehmann H, Aurich S, Bauer N, Melzer M, Moellerberndt J, Patané V, Schnabel CL, Burk J. Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture. Front Bioeng Biotechnol 2020;8:613621.
              doi: 10.3389/fbioe.2020.613621pubmed: 33553119google scholar: lookup
            18. Velloso Alvarez A, Boone LH, Braim AP, Taintor JS, Caldwell F, Wright JC, Wooldridge AA. A Survey of Clinical Usage of Non-steroidal Intra-Articular Therapeutics by Equine Practitioners. Front Vet Sci 2020;7:579967.
              doi: 10.3389/fvets.2020.579967pubmed: 33195592google scholar: lookup
            19. Perinelli DR, Bonacucina G, Pucciarelli S, Cespi M, Serri E, Polzonetti V, Tambella AM, Vincenzetti S. Rheological Properties and Growth Factors Content of Platelet-Rich Plasma: Relevance in Veterinary Biomedical Treatments. Biomedicines 2020 Oct 18;8(10).
              doi: 10.3390/biomedicines8100429pubmed: 33081006google scholar: lookup
            20. Perego R, Spada E, Baggiani L, Martino PA, Proverbio D. Efficacy of a Semi Automated Commercial Closed System for Autologous Leukocyte- and Platelet-Rich Plasma (l-prp) Production in Dogs: A Preliminary Study. Animals (Basel) 2020 Aug 4;10(8).
              doi: 10.3390/ani10081342pubmed: 32759643google scholar: lookup
            21. Gilbertie JM, Schaer TP, Schubert AG, Jacob ME, Menegatti S, Ashton Lavoie R, Schnabel LV. Platelet-rich plasma lysate displays antibiofilm properties and restores antimicrobial activity against synovial fluid biofilms in vitro. J Orthop Res 2020 Jun;38(6):1365-1374.
              doi: 10.1002/jor.24584pubmed: 31922274google scholar: lookup
            22. Tambella AM, Attili AR, Dupré G, Cantalamessa A, Martin S, Cuteri V, Marcazzan S, Del Fabbro M. Platelet-rich plasma to treat experimentally-induced skin wounds in animals: A systematic review and meta-analysis. PLoS One 2018;13(1):e0191093.
              doi: 10.1371/journal.pone.0191093pubmed: 29324848google scholar: lookup
            23. Carmona JU, Ríos DL, López C, Álvarez ME, Pérez JE. Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide. Vet Med Int 2017;2017:6059485.
              doi: 10.1155/2017/6059485pubmed: 28761774google scholar: lookup
            24. Franklin SP, Birdwhistell KE, Strelchik A, Garner BC, Brainard BM. Influence of Cellular Composition and Exogenous Activation on Growth Factor and Cytokine Concentrations in Canine Platelet-Rich Plasmas. Front Vet Sci 2017;4:40.
              doi: 10.3389/fvets.2017.00040pubmed: 28424777google scholar: lookup
            25. Hauschild G, Geburek F, Gosheger G, Eveslage M, Serrano D, Streitbürger A, Johannlükens S, Menzel D, Mischke R. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations. BMC Vet Res 2017 Jan 5;13(1):7.
              doi: 10.1186/s12917-016-0920-4pubmed: 28056978google scholar: lookup
            26. Geburek F, Gaus M, van Schie HT, Rohn K, Stadler PM. Effect of intralesional platelet-rich plasma (PRP) treatment on clinical and ultrasonographic parameters in equine naturally occurring superficial digital flexor tendinopathies - a randomized prospective controlled clinical trial. BMC Vet Res 2016 Sep 7;12(1):191.
              doi: 10.1186/s12917-016-0826-1pubmed: 27604193google scholar: lookup
            27. Rinnovati R, Romagnoli N, Gentilini F, Lambertini C, Spadari A. The influence of environmental variables on platelet concentration in horse platelet-rich plasma. Acta Vet Scand 2016 Jul 4;58(1):45.
              doi: 10.1186/s13028-016-0226-3pubmed: 27377748google scholar: lookup
            28. Carmona JU, Ríos DL, López C, Álvarez ME, Pérez JE, Bohórquez ME. In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide. BMC Vet Res 2016 Jul 1;12(1):135.
              doi: 10.1186/s12917-016-0759-8pubmed: 27369779google scholar: lookup
            29. de Souza MV, Silva MB, Pinto Jde O, Lima MB, Crepaldi J, Lopes GF, dos Santos HB, Ribeiro RI, Thomé RG. Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich Plasma. Biomed Res Int 2015;2015:893485.
              doi: 10.1155/2015/893485pubmed: 26236743google scholar: lookup
            30. Schnabel CL, Steinig P, Koy M, Schuberth HJ, Juhls C, Oswald D, Wittig B, Willenbrock S, Murua Escobar H, Pfarrer C, Wagner B, Jaehnig P, Moritz A, Feige K, Cavalleri JM. Immune response of healthy horses to DNA constructs formulated with a cationic lipid transfection reagent. BMC Vet Res 2015 Jun 23;11:140.
              doi: 10.1186/s12917-015-0452-3pubmed: 26100265google scholar: lookup
            31. Ríos DL, López C, Álvarez ME, Samudio IJ, Carmona JU. Effects over time of two platelet gel supernatants on growth factor, cytokine and hyaluronan concentrations in normal synovial membrane explants challenged with lipopolysaccharide. BMC Musculoskelet Disord 2015 Jun 20;16:153.
              doi: 10.1186/s12891-015-0605-3pubmed: 26092588google scholar: lookup
            32. Ríos DL, López C, Carmona JU. Platelet-Rich Gel Supernatants Stimulate the Release of Anti-Inflammatory Proteins on Culture Media of Normal Equine Synovial Membrane Explants. Vet Med Int 2015;2015:547052.
              doi: 10.1155/2015/547052pubmed: 26090267google scholar: lookup
            33. Di Ianni F, Merli E, Burtini F, Conti V, Pelizzone I, Di Lecce R, Parmigiani E, Squassino GP, Del Bue M, Lucarelli E, Ramoni R, Grolli S. Preparation and application of an innovative thrombocyte/leukocyte-enriched plasma to promote tissue repair in chelonians. PLoS One 2015;10(4):e0122595.
              doi: 10.1371/journal.pone.0122595pubmed: 25901960google scholar: lookup
            34. Brossi PM, Moreira JJ, Machado TS, Baccarin RY. Platelet-rich plasma in orthopedic therapy: a comparative systematic review of clinical and experimental data in equine and human musculoskeletal lesions. BMC Vet Res 2015 Apr 22;11:98.
              doi: 10.1186/s12917-015-0403-zpubmed: 25896610google scholar: lookup
            35. Giraldo CE, Álvarez ME, Carmona JU. Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res 2015 Mar 14;11:60.
              doi: 10.1186/s12917-015-0370-4pubmed: 25889052google scholar: lookup
            36. López C, Alvarez ME, Carmona JU. Temporal Bacteriostatic Effect and Growth Factor Loss in Equine Platelet Components and Plasma Cultured with Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus: A Comparative In Vitro Study. Vet Med Int 2014;2014:525826.
              doi: 10.1155/2014/525826pubmed: 25506468google scholar: lookup
            37. Fukaya M, Ito A. A New Economic Method for Preparing Platelet-rich Plasma. Plast Reconstr Surg Glob Open 2014 Jun;2(6):e162.
              doi: 10.1097/GOX.0000000000000109pubmed: 25289355google scholar: lookup
            38. Roffi A, Filardo G, Assirelli E, Cavallo C, Cenacchi A, Facchini A, Grigolo B, Kon E, Mariani E, Pratelli L, Pulsatelli L, Marcacci M. Does platelet-rich plasma freeze-thawing influence growth factor release and their effects on chondrocytes and synoviocytes?. Biomed Res Int 2014;2014:692913.
              doi: 10.1155/2014/692913pubmed: 25136613google scholar: lookup
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