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Veterinary parasitology2007; 147(1-2); 155-160; doi: 10.1016/j.vetpar.2007.02.037

Efficacy of 2,6-dichlorophenol lure to control Dermacentor nitens.

Abstract: This study was carried out with the objective of evaluating the efficacy of a 2,6-dichlorophenol (2,6-DCP) lure to control Dermacentor nitens (Acari: Ixodidae). Slow-release formulations of the pheromone formulated with and without cypermethrin were prepared. Olfactometer bioassays were used to define the best dose of the pheromone and to evaluate the effect of cypermethrin with 2,6-DCP attractiveness. Sexually active males were released 15 cm from 2 cmx1 cm pieces of polypropylene treated with different odors: 2,6-DCP in a liposphere system (1.5, 30 and 300 microg--without cypermethrin and 30 microg--with cypermethrin) and 2,6-DCP in hexane (30 microg). The tests were conducted 7 and 15 days after the preparation of the odor sources. The percentages of males that oriented, or showed directional movement toward the stimulus, and their tracks were recorded. Lures (10 cmx2 cm pieces of polypropylene) treated with the best dose of the liposphere formulation (30 microg) were prepared. The lures were evaluated in horses that had been artificially infested with D. nitens. The horses' ears were infested with 3000 D. nitens larvae per ear, once weekly for 4 weeks. The animals were divided into three groups: control, 2,6-DCP and 2,6-DCP+cypermethrin. On day 0, the lures of their respective treatments were attached to the horses' napes. From days 6 to 20 after attachment, female ticks of 4 mm or over in length were counted on the ears, every 2 or 3 days. Olfactometer analysis showed higher orientation to 30 microg dose and more prolonged release of the pheromone in the liposphere formulation than in hexane; cypermethrin did not interfere with the attractive effect of the pheromone. The lures were efficient in the first 10 days after attachment, when the mean number of females was higher in the control group (24.9) than in 2,6-DCP and cypermethrin (5.4), and 2,6-DCP (9.2) groups. After that period the number of females was similar in the control and treated groups. These results indicate that 2,6-DCP lures used in a liposphere formulation can control D. nitens for at least 10 days by preventing its copulation. However, further evaluation of longer-term pheromone release under natural conditions is needed in order to validate this kind of control. In addition, the use of extra lures on the horse's tail may help to control populations on the hindquarters.
Publication Date: 2007-04-24 PubMed ID: 17459588DOI: 10.1016/j.vetpar.2007.02.037Google Scholar: Lookup
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  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This research article examines the effectiveness of 2,6-dichlorophenol (2,6-DCP) as a lure to control Dermacentor nitens, a species of tick. The study found that the 2,6-DCP lure in a specific formulation could control D. nitens for at least 10 days, and suggested further evaluation for long-term effectiveness.

Objectives and Methods of the Research

  • The study aimed to evaluate the efficacy of a 2,6-DCP lure in controlling Dermacentor nitens (D. nitens), a tick species. Slow-release formulations of the pheromone, both with and without the insecticide cypermethrin, were prepared.
  • Olfactometer bioassays were used to determine the most effective dose of the pheromone and to evaluate whether cypermethrin influences the attractiveness of 2,6-DCP to the ticks.
  • Sexually active male ticks were exposed to polypropylene pieces treated with different concentrations of 2,6-DCP, either in a liposphere system or in hexane. The attracted ticks were recorded.

Results from the Laboratory Tests

  • The 30 microg dose of 2,6-DCP in the liposphere formulation showed the highest rate of tick attraction and more prolonged pheromone release than the hexane formulation.
  • The addition of cypermethrin did not appear to interfere with the attractiveness of the 2,6-DCP lure.

Field Tests and Findings

  • After identifying the most effective pheromone formulation and dose, field tests were conducted on horses artificially infested with D. nitens. The lures were attached to the horses’ napes.
  • In the first ten days of testing, the number of female ticks on the horses that received the 2,6-DCP treatments was significantly lower than that in the control group, indicating that the lures were effective in controlling the ticks.
  • After ten days, however, the number of female ticks was similar in both the control group and the treated groups, suggesting that the effectiveness of the treatment diminished over time.

Conclusions and Future Suggestions

  • The results suggest that 2,6-DCP lures, specifically in a liposphere formulation, can control D. nitens for at least ten days by preventing copulation.
  • The study indicates the need for further evaluation of the long-term efficacy of the pheromone under natural conditions to validate this control method.
  • The researchers also suggest exploring the use of additional lures, such as on a horse’s tail, as an extra measure to control tick populations on the hindquarters of the animal.

Cite This Article

APA
Borges LM, Ferreira LA, da Silva LS, de Oliveira RA, Mussi SV, Faria KA, Melo LS, Abud LJ, Costa GL, Soares SF. (2007). Efficacy of 2,6-dichlorophenol lure to control Dermacentor nitens. Vet Parasitol, 147(1-2), 155-160. https://doi.org/10.1016/j.vetpar.2007.02.037

Publication

ISSN: 0304-4017
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 147
Issue: 1-2
Pages: 155-160

Researcher Affiliations

Borges, Lígia Miranda Ferreira
  • DMIPP, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235, s/n, Setor Universitário, CEP 74001-970, Goiânia, GO, Brazil. ligia@iptsp.ufg.br
Ferreira, Lucas Antônio Miranda
    da Silva, Luciano Schneider
      de Oliveira, Rodrigo Arruda
        Mussi, Samuel Vidal
          Faria, Karina Alves
            Melo, Lorena Socorre
              Abud, Lucas Jacomin
                Costa, Gustavo Lage
                  Soares, Sara Fernandes

                    MeSH Terms

                    • Animals
                    • Chlorophenols / pharmacology
                    • Dermacentor
                    • Female
                    • Horse Diseases / parasitology
                    • Horse Diseases / prevention & control
                    • Horses
                    • Insecticides
                    • Male
                    • Pheromones / pharmacology
                    • Pyrethrins
                    • Tick Control / methods
                    • Tick Control / standards
                    • Tick Infestations / prevention & control
                    • Tick Infestations / veterinary
                    • Time Factors

                    Citations

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