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Home / Equine Research Bank / Sci Rep / Elastin is Localised to the Interfascicular Matrix of Energy...
Scientific reports2017; 7(1); 9713; doi: 10.1038/s41598-017-09995-4

Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing.

Abstract: Tendon is composed of fascicles bound together by the interfascicular matrix (IFM). Energy storing tendons are more elastic and extensible than positional tendons; behaviour provided by specialisation of the IFM to enable repeated interfascicular sliding and recoil. With ageing, the IFM becomes stiffer and less fatigue resistant, potentially explaining why older tendons become more injury-prone. Recent data indicates enrichment of elastin within the IFM, but this has yet to be quantified. We hypothesised that elastin is more prevalent in energy storing than positional tendons, and is mainly localised to the IFM. Further, we hypothesised that elastin becomes disorganised and fragmented, and decreases in amount with ageing, especially in energy storing tendons. Biochemical analyses and immunohistochemical techniques were used to determine elastin content and organisation, in young and old equine energy storing and positional tendons. Supporting the hypothesis, elastin localises to the IFM of energy storing tendons, reducing in quantity and becoming more disorganised with ageing. These changes may contribute to the increased injury risk in aged energy storing tendons. Full understanding of the processes leading to loss of elastin and its disorganisation with ageing may aid in the development of treatments to prevent age related tendinopathy.
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Publication Date: 2017-08-30 PubMed ID: 28855560PubMed Central: PMC5577209DOI: 10.1038/s41598-017-09995-4Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the role of Elastin in the inter-facial matrix (IFM) of energy-storing tendons and investigated changes in its amount and organization as tendons age. Findings suggest that elastin present in the IFM of energy-storing tendons decrease and become disorganized with aging, leading to increased injury risk in old age.

Study Rationale

  • The study aimed to explore the composition of tendons, specifically focussing on the interfascicular matrix (IFM). Tendons are made up of fascicles, or bundles of fibers, bound together by this IFM.
  • The research was based on the premise that energy storing tendons, due to their elasticity and extensibility, are more susceptible to repeated interfascicular sliding and recoil. This behaviour of energy storing tendons steers their propensity to grow stiffer with age, thereby increasing the risk of injuries in older tendons.
  • The study aimed to provide a quantifiable perspective on the content of elastin in the IFM, given that recent data suggested an abundance of this protein in the IFM.

Hypotheses and Methods

  • The study put forth two hypotheses: the presence of elastin is more pronounced in energy storing tendons compared to positional tendons and primarily localised within the IFM. Also, the researchers hypothesised that elastin becomes disorganised and decreases in quantity with ageing, especially in the energy storing tendons.
  • They employed biochemical analyses and immunohistochemical techniques to understand both the organization and content of elastin in young and old equine energy storing and positional tendons.

Findings and Implications

  • The research findings supported the proposed hypotheses. It confirmed that elastin primarily localises to the IFM in energy storing tendons, and diminishes in quantity and becomes disorganized as ageing progresses.
  • This change in the elastin content and its organization with ageing may contribute to the increased risk of injuries in energy storing tendons. This understanding might help develop treatments to prevent age-related tendinopathy.

Cite This Article

APA
Godinho MSC, Thorpe CT, Greenwald SE, Screen HRC. (2017). Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing. Sci Rep, 7(1), 9713. https://doi.org/10.1038/s41598-017-09995-4

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 7
Issue: 1
Pages: 9713
PII: 9713

Researcher Affiliations

Godinho, Marta S C
  • Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, United Kingdom.
Thorpe, Chavaunne T
  • Comparative Biomedical Sciences, The Royal Veterinary College, Royal College Street, London, NW1 0TU, United Kingdom.
Greenwald, Steve E
  • Blizard Institute, Barts and London School of Medicine and Dentistry, Turner Street, London, E1 11BB, United Kingdom.
Screen, Hazel R C
  • Institute of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, United Kingdom. h.r.c.screen@qmul.ac.uk.

MeSH Terms

  • Aging / metabolism
  • Animals
  • Desmosine / metabolism
  • Elastin / metabolism
  • Extracellular Matrix / metabolism
  • Fascia / metabolism
  • Fluorescent Antibody Technique
  • Horses
  • Tendinopathy / etiology
  • Tendinopathy / metabolism
  • Tendinopathy / pathology
  • Tendons / metabolism
  • Tendons / pathology

Grant Funding

  • BB/K008412/1 / Biotechnology and Biological Sciences Research Council

Conflict of Interest Statement

The authors declare that they have no competing interests.

References

This article includes 53 references
  1. Alexander RM. Energy-saving mechanisms in walking and running.. J Exp Biol 1991 Oct;160:55-69.
    pubmed: 1960518doi: 10.1242/jeb.160.1.55google scholar: lookup
  2. Benjamin M, Ralphs JR. Tendons and ligaments--an overview.. Histol Histopathol 1997 Oct;12(4):1135-44.
    pubmed: 9302572
  3. Kubo K, Kawakami Y, Kanehisa H, Fukunaga T. Measurement of viscoelastic properties of tendon structures in vivo.. Scand J Med Sci Sports 2002 Feb;12(1):3-8.
    doi: 10.1034/j.1600-0838.2002.120102.xpubmed: 11985759google scholar: lookup
  4. Ker R. Mechanics of tendon, from an engineering perspective.. Int. J. Fatigue 2007;29:1001–1009.
    doi: 10.1016/j.ijfatigue.2006.09.020google scholar: lookup
  5. Thorpe CT, Clegg PD, Birch HL. A review of tendon injury: why is the equine superficial digital flexor tendon most at risk?. Equine Vet J 2010 Mar;42(2):174-80.
    doi: 10.2746/042516409X480395pubmed: 20156256google scholar: lookup
  6. Shepherd JH, Legerlotz K, Demirci T, Klemt C, Riley GP, Screen HR. Functionally distinct tendon fascicles exhibit different creep and stress relaxation behaviour.. Proc Inst Mech Eng H 2014 Jan;228(1):49-59.
    pmc: PMC4361498pubmed: 24285289doi: 10.1177/0954411913509977google scholar: lookup
  7. Lui PP, Maffulli N, Rolf C, Smith RK. What are the validated animal models for tendinopathy?. Scand J Med Sci Sports 2011 Feb;21(1):3-17.
    doi: 10.1111/j.1600-0838.2010.01164.xpubmed: 20673247google scholar: lookup
  8. Innes JF, Clegg P. Comparative rheumatology: what can be learnt from naturally occurring musculoskeletal disorders in domestic animals?. Rheumatology (Oxford) 2010 Jun;49(6):1030-9.
    doi: 10.1093/rheumatology/kep465pubmed: 20176567google scholar: lookup
  9. Birch HL, Bailey JV, Bailey AJ, Goodship AE. Age-related changes to the molecular and cellular components of equine flexor tendons.. Equine Vet J 1999 Sep;31(5):391-6.
    doi: 10.1111/j.2042-3306.1999.tb03838.xpubmed: 10505954google scholar: lookup
  10. Thorpe CT, Udeze CP, Birch HL, Clegg PD, Screen HR. Specialization of tendon mechanical properties results from interfascicular differences.. J R Soc Interface 2012 Nov 7;9(76):3108-17.
    doi: 10.1098/rsif.2012.0362pmc: PMC3479922pubmed: 22764132google scholar: lookup
  11. Thorpe CT, Udeze CP, Birch HL, Clegg PD, Screen HR. Capacity for sliding between tendon fascicles decreases with ageing in injury prone equine tendons: a possible mechanism for age-related tendinopathy?. Eur Cell Mater 2013 Jan 8;25:48-60.
    doi: 10.22203/eCM.v025a04pubmed: 23300032google scholar: lookup
  12. Thorpe CT, Peffers MJ, Simpson D, Halliwell E, Screen HR, Clegg PD. Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition.. Sci Rep 2016 Feb 4;6:20455.
    doi: 10.1038/srep20455pmc: PMC4740843pubmed: 26842662google scholar: lookup
  13. Kannus P. Structure of the tendon connective tissue.. Scand J Med Sci Sports 2000 Dec;10(6):312-20.
    doi: 10.1034/j.1600-0838.2000.010006312.xpubmed: 11085557google scholar: lookup
  14. Fallon J, Blevins FT, Vogel K, Trotter J. Functional morphology of the supraspinatus tendon.. J Orthop Res 2002 Sep;20(5):920-6.
    doi: 10.1016/S0736-0266(02)00032-2pubmed: 12382954google scholar: lookup
  15. Grant TM, Thompson MS, Urban J, Yu J. Elastic fibres are broadly distributed in tendon and highly localized around tenocytes.. J Anat 2013 Jun;222(6):573-9.
    doi: 10.1111/joa.12048pmc: PMC3666236pubmed: 23587025google scholar: lookup
  16. Thorpe CT, Karunaseelan KJ, Ng Chieng Hin J, Riley GP, Birch HL, Clegg PD, Screen HR. Distribution of proteins within different compartments of tendon varies according to tendon type.. J Anat 2016 Sep;229(3):450-8.
    doi: 10.1111/joa.12485pmc: PMC4974547pubmed: 27113131google scholar: lookup
  17. Smith KD, Vaughan-Thomas A, Spiller DG, Innes JF, Clegg PD, Comerford EJ. The organisation of elastin and fibrillins 1 and 2 in the cruciate ligament complex.. J Anat 2011 Jun;218(6):600-7.
    doi: 10.1111/j.1469-7580.2011.01374.xpmc: PMC3125893pubmed: 21466551google scholar: lookup
  18. Wagenseil JE, Mecham RP. Elastin in large artery stiffness and hypertension.. J Cardiovasc Transl Res 2012 Jun;5(3):264-73.
    doi: 10.1007/s12265-012-9349-8pmc: PMC3383658pubmed: 22290157google scholar: lookup
  19. PIERCE JA, HOCOTT JB. Studies on the collagen and elastin content of the human lung.. J Clin Invest 1960 Jan;39(1):8-14.
    doi: 10.1172/JCI104030pmc: PMC290656pubmed: 14432825google scholar: lookup
  20. Braverman IM, Fonferko E. Studies in cutaneous aging: I. The elastic fiber network.. J Invest Dermatol 1982 May;78(5):434-43.
    doi: 10.1111/1523-1747.ep12507866pubmed: 7069221google scholar: lookup
  21. Oxlund H, Manschot J, Viidik A. The role of elastin in the mechanical properties of skin.. J Biomech 1988;21(3):213-8.
    doi: 10.1016/0021-9290(88)90172-8pubmed: 3379082google scholar: lookup
  22. Foster JA, Bruenger E, Gray WR, Sandberg LB. Isolation and amino acid sequences of tropoelastin peptides.. J Biol Chem 1973 Apr 25;248(8):2876-9.
    pubmed: 4697396
  23. Foster JA, Rubin L, Kagan HM, Franzblau C, Bruenger E, Sandberg LB. Isolation and characterization of cross-linked peptides from elastin.. J Biol Chem 1974 Oct 10;249(19):6191-6.
    pubmed: 4472530
  24. Ramirez F, Pereira L. The fibrillins.. Int J Biochem Cell Biol 1999 Feb;31(2):255-9.
    doi: 10.1016/S1357-2725(98)00109-5pubmed: 10216958google scholar: lookup
  25. Vrhovski B, Weiss AS. Biochemistry of tropoelastin.. Eur J Biochem 1998 Nov 15;258(1):1-18.
    doi: 10.1046/j.1432-1327.1998.2580001.xpubmed: 9851686google scholar: lookup
  26. Bedell-Hogan D, Trackman P, Abrams W, Rosenbloom J, Kagan H. Oxidation, cross-linking, and insolubilization of recombinant tropoelastin by purified lysyl oxidase.. J Biol Chem 1993 May 15;268(14):10345-50.
    pubmed: 8098038
  27. Akagawa M, Suyama K. Mechanism of formation of elastin crosslinks.. Connect Tissue Res 2000;41(2):131-41.
    doi: 10.3109/03008200009067665pubmed: 10992159google scholar: lookup
  28. Robert L, Robert AM, Fülöp T. Rapid increase in human life expectancy: will it soon be limited by the aging of elastin?. Biogerontology 2008 Apr;9(2):119-33.
    doi: 10.1007/s10522-007-9122-6pubmed: 18175202google scholar: lookup
  29. Luisetti M, Ma S, Iadarola P, Stone PJ, Viglio S, Casado B, Lin YY, Snider GL, Turino GM. Desmosine as a biomarker of elastin degradation in COPD: current status and future directions.. Eur Respir J 2008 Nov;32(5):1146-57.
    doi: 10.1183/09031936.00174807pubmed: 18978133google scholar: lookup
  30. Sherratt MJ. Tissue elasticity and the ageing elastic fibre.. Age (Dordr) 2009 Dec;31(4):305-25.
    doi: 10.1007/s11357-009-9103-6pmc: PMC2813052pubmed: 19588272google scholar: lookup
  31. Lee TC, Midura RJ, Hascall VC, Vesely I. The effect of elastin damage on the mechanics of the aortic valve.. J Biomech 2001 Feb;34(2):203-10.
    doi: 10.1016/S0021-9290(00)00187-1pubmed: 11165284google scholar: lookup
  32. Heinz A, Taddese S, Sippl W, Neubert RH, Schmelzer CE. Insights into the degradation of human elastin by matrilysin-1.. Biochimie 2011 Feb;93(2):187-94.
    doi: 10.1016/j.biochi.2010.09.011pubmed: 20884320google scholar: lookup
  33. Viera AJ, Garrett JM. Understanding interobserver agreement: the kappa statistic.. Fam Med 2005 May;37(5):360-3.
    pubmed: 15883903
  34. Thorpe CT, Chaudhry S, Lei II, Varone A, Riley GP, Birch HL, Clegg PD, Screen HR. Tendon overload results in alterations in cell shape and increased markers of inflammation and matrix degradation.. Scand J Med Sci Sports 2015 Aug;25(4):e381-91.
    pubmed: 25639911doi: 10.1111/sms.12333google scholar: lookup
  35. Tempfer H, Traweger A. Tendon Vasculature in Health and Disease.. Front Physiol 2015;6:330.
    doi: 10.3389/fphys.2015.00330pmc: PMC4650849pubmed: 26635616google scholar: lookup
  36. Fenwick SA, Hazleman BL, Riley GP. The vasculature and its role in the damaged and healing tendon.. Arthritis Res 2002;4(4):252-60.
    doi: 10.1186/ar416pmc: PMC128932pubmed: 12106496google scholar: lookup
  37. Liu ZQ. Scale space approach to directional analysis of images.. Appl Opt 1991 Apr 10;30(11):1369-73.
    doi: 10.1364/AO.30.001369pubmed: 20700292google scholar: lookup
  38. Berens P. CircStat: A MATLAB Toolbox for Circular Statistics.. J. Stat. Softw. 2009;31:1–21.
    doi: 10.18637/jss.v031.i10google scholar: lookup
  39. Dakin SG, Smith RK, Heinegård D, Önnerfjord P, Khabut A, Dudhia J. Proteomic analysis of tendon extracellular matrix reveals disease stage-specific fragmentation and differential cleavage of COMP (cartilage oligomeric matrix protein).. J Biol Chem 2014 Feb 21;289(8):4919-27.
    doi: 10.1074/jbc.M113.511972pmc: PMC3931053pubmed: 24398684google scholar: lookup
  40. Robert L, Robert AM, Fülöp T. Rapid increase in human life expectancy: will it soon be limited by the aging of elastin?. Biogerontology 2008 Apr;9(2):119-33.
    doi: 10.1007/s10522-007-9122-6pubmed: 18175202google scholar: lookup
  41. Greenwald SE. Ageing of the conduit arteries.. J Pathol 2007 Jan;211(2):157-72.
    doi: 10.1002/path.2101pubmed: 17200940google scholar: lookup
  42. Thorpe CT, Riley GP, Birch HL, Clegg PD, Screen HRC. Fascicles and the interfascicular matrix show adaptation for fatigue resistance in energy storing tendons.. Acta Biomater 2016 Sep 15;42:308-315.
    doi: 10.1016/j.actbio.2016.06.012pmc: PMC5015572pubmed: 27286677google scholar: lookup
  43. Thorpe CT, Godinho MSC, Riley GP, Birch HL, Clegg PD, Screen HRC. The interfascicular matrix enables fascicle sliding and recovery in tendon, and behaves more elastically in energy storing tendons.. J Mech Behav Biomed Mater 2015 Dec;52:85-94.
    doi: 10.1016/j.jmbbm.2015.04.009pmc: PMC4655227pubmed: 25958330google scholar: lookup
  44. Thorpe CT, Riley GP, Birch HL, Clegg PD, Screen HRC. Fascicles and the interfascicular matrix show adaptation for fatigue resistance in energy storing tendons.. Acta Biomater 2016 Sep 15;42:308-315.
    doi: 10.1016/j.actbio.2016.06.012pmc: PMC5015572pubmed: 27286677google scholar: lookup
  45. Anwar, R. A. In Biochemical Education18, 162–166 (1990).
  46. Batson EL, Paramour RJ, Smith TJ, Birch HL, Patterson-Kane JC, Goodship AE. Are the material properties and matrix composition of equine flexor and extensor tendons determined by their functions?. Equine Vet J 2003 May;35(3):314-8.
    doi: 10.2746/042516403776148327pubmed: 12755437google scholar: lookup
  47. Grant TM, Yapp C, Chen Q, Czernuszka JT, Thompson MS. The Mechanical, Structural, and Compositional Changes of Tendon Exposed to Elastase.. Ann Biomed Eng 2015 Oct;43(10):2477-86.
    doi: 10.1007/s10439-015-1308-5pubmed: 25808209google scholar: lookup
  48. Nimeskern L, Utomo L, Lehtoviita I, Fessel G, Snedeker JG, van Osch GJ, Müller R, Stok KS. Tissue composition regulates distinct viscoelastic responses in auricular and articular cartilage.. J Biomech 2016 Feb 8;49(3):344-52.
    doi: 10.1016/j.jbiomech.2015.12.032pubmed: 26772799google scholar: lookup
  49. Birch HL, Worboys S, Eissa S, Jackson B, Strassburg S, Clegg PD. Matrix metabolism rate differs in functionally distinct tendons.. Matrix Biol 2008 Apr;27(3):182-9.
    pubmed: 18032005doi: 10.1016/j.matbio.2007.10.004google scholar: lookup
  50. Gunja-Smith Z. An enzyme-linked immunosorbent assay to quantitate the elastin crosslink desmosine in tissue and urine samples.. Anal Biochem 1985 May 15;147(1):258-64.
    doi: 10.1016/0003-2697(85)90036-3pubmed: 4025822google scholar: lookup
  51. Watanabe T, Ishimori K, Verplanke AJ, Matsuki H, Kasuga H. An enzyme-linked immunosorbent assay (ELISA) for the quantitation of urinary desmosine.. Tokai J Exp Clin Med 1989 Sep;14(4):347-56.
    pubmed: 2487974
  52. Csóka TB, Frost GI, Stern R. Hyaluronidases in tissue invasion.. Invasion Metastasis 1997;17(6):297-311.
    pubmed: 9949289
  53. Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez JY, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A. Fiji: an open-source platform for biological-image analysis.. Nat Methods 2012 Jun 28;9(7):676-82.
    pmc: PMC3855844pubmed: 22743772doi: 10.1038/nmeth.2019google scholar: lookup

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