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Home / Equine Research Bank / PLoS One / Endobronchial Ultrasound Reliably Quantifies Airway Smooth M...
PloS one2015; 10(9); e0136284; doi: 10.1371/journal.pone.0136284

Endobronchial Ultrasound Reliably Quantifies Airway Smooth Muscle Remodeling in an Equine Asthma Model.

Abstract: Endobronchial ultrasonography (EBUS) revealed differences in the thickness of the layer representing subepithelial tissues (L2) between human asthmatics and controls, but whether this measurement correlates with airway smooth muscle (ASM) remodeling in asthma is unknown. In this study, we sought to determine the ability of EBUS to predict histological ASM remodeling in normal and equine asthmatic airways. We studied 109 isolated bronchi from the lungs of 13 horses. They underwent EBUS examination using a 30 MHz radial probe before being processed for histology. ASM remodeling parameters were evaluated in EBUS images (L2 thickness, L2 area, L2 area/internal perimeter [Pi] and L2 area/Pi2) and histological cuts (ASM area/Pi2), and compared. EBUS was then performed ex vivo on the lungs of 4 horses with heaves, an asthma-like condition of horses, and 7 controls to determine whether central bronchial remodeling could be detected with this technique. An optimized approach was developed based on data variability within airways, subjects, and groups, and then validated in 7 horses (3 controls, 4 with heaves) that underwent EBUS in vivo. L2 area was significantly associated to ASM area in isolated lungs (p<0.0001), in the absence of significant bias related to the airway size. Bronchial size significantly affected EBUS ASM-related parameters, except for L2 area/Pi2. L2 area/Pi2 was increased in the airways of asthmatic horses compared to controls, both ex vivo and in vivo (p<0.05). Bronchial histology confirmed our findings (AASM/Pi2 was increased in asthmatic horses compared to controls, p<0.05). In both horses with heaves and controls, L2 was composed of ASM for the outer 75% of its thickness and by ECM for the remaining inner 25%. In conclusion, EBUS reliably allows assessment of asthma-associated ASM remodeling of central airways in a non-invasive way.
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Publication Date: 2015-09-08 PubMed ID: 26348727PubMed Central: PMC4562526DOI: 10.1371/journal.pone.0136284Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study that evaluates the effectiveness of Endobronchial Ultrasonography (EBUS) for predicting airway smooth muscle (ASM) remodeling in equine asthmatic airways.

Objective of the Research

  • The main goal of the research was to assess whether EBUS could provide an accurate indication of ASM remodeling. This phenomenon, which involves changes in the structure of the airway smooth muscle, is associated with asthma.

Research Method

  • To achieve this, the researchers examined 109 isolated bronchi from the lungs of 13 horses, using EBUS with a 30 MHz radial probe before proceeding to histology.
  • ASM remodeling parameters were assessed in both EBUS images and histological cuts, then compared.
  • Further testing with EBUS was performed ex vivo on the lungs of four horses with heaves (a condition similar to asthma) and seven control horses.

Findings

  • The study discovered that there was a significant correlation between L2 area and ASM area in the isolated lungs.
  • The study also found that while bronchial size significantly impacted EBUS ASM-related parameters, it did not significantly affect the L2 area/Pi2.
  • Moreover, the L2 area/Pi2 was found to be increased in the airways of asthmatic horses compared to control horses, both ex vivo and in vivo.
  • A histological examination of bronchial tissue confirmed these findings.
  • Finally, in both control horses and those with heaves, ASM made up about 75% of the L2 thickness, while the extracellular matrix (ECM) comprised the remaining 25%.

Conclusion

  • The researchers concluded that EBUS can reliably assess ASM remodeling of central airways in a non-invasive way, which is associated with asthma.

Cite This Article

APA
Bullone M, Beauchamp G, Godbout M, Martin JG, Lavoie JP. (2015). Endobronchial Ultrasound Reliably Quantifies Airway Smooth Muscle Remodeling in an Equine Asthma Model. PLoS One, 10(9), e0136284. https://doi.org/10.1371/journal.pone.0136284

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 10
Issue: 9
Pages: e0136284
PII: e0136284

Researcher Affiliations

Bullone, Michela
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Q, Canada.
Beauchamp, Guy
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Q, Canada.
Godbout, Mireille
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Q, Canada.
Martin, James G
  • Meakins-Christie Laboratories, Department of Medicine, McGill University, Montreal, Q, Canada.
Lavoie, Jean-Pierre
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Q, Canada.

MeSH Terms

  • Airway Remodeling
  • Animals
  • Asthma / diagnostic imaging
  • Asthma / pathology
  • Bronchi / diagnostic imaging
  • Cell Proliferation / physiology
  • Diagnostic Imaging
  • Disease Models, Animal
  • Horses
  • Humans
  • Lung / diagnostic imaging
  • Lung / pathology
  • Muscle, Smooth / diagnostic imaging
  • Ultrasonics
  • Ultrasonography

Grant Funding

  • R0017988 / Canadian Institutes of Health Research

Conflict of Interest Statement

The authors have declared that no competing interests exist.

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This article has been cited 13 times.
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