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Home / Equine Research Bank / Probiotics Antimicrob Proteins / Enterocin M and its Beneficial Effects in Horses-a Pilot Exp...
Probiotics and antimicrobial proteins2018; 10(3); 420-426; doi: 10.1007/s12602-018-9390-2

Enterocin M and its Beneficial Effects in Horses-a Pilot Experiment.

Abstract: Probiotic bacteria or their antimicrobial proteinaceous substances called bacteriocins (enterocins) hold promising prophylactic potential for animal breeding. This study present the results achieved after application of Enterocin M in horses. Enterocin M has never been applied to horses before. Clinically healthy horses (10) were involved in this pilot experiment. They were placed in the stables of the University of Veterinary Medicine and Pharmacy, Košice, Slovakia, with the approval of the University Ethics Committee. The animals were fed twice a day with hay and oats, or alternatively grazed with access to water ad libitum. The experiment lasted 6 weeks. Sampling was performed at the start of the experiment, at day 0-1, at day 21 (3 weeks of Enterocin M application), and at day 42 (3 weeks of cessation). Feces were sampled directly from the rectum and blood from the vena jugularis; the samples were immediately treated and/or stored for analyses. Each horse itself represented a control animal (compared to its status at the start of the experiment, day 0-1). After initial sampling, the horses were administered 100 μl of Ent M (precipitate, 12,800 AU/ml) in a small feed bolus to ensure it was consumed; Ent M was applied for 3 weeks (21 days). Fecal samples were treated using the standard microbial dilution method; phagocytic activity was assessed with standard and flow cytometry; biochemistry and metabolic profiles were tested using commercial kits and standard methods. Administration of Ent M led to mathematical reduction of coliforms, campylobacters (P < 0.05), and significant reduction of Clostridium spp. (P < 0.001, P < 0.001); increase of PA values was noted (P < 0.05, P < 0.0001); no negative influence on hydrolytic enzyme profile or biochemical blood parameters was noted.
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Publication Date: 2018-02-09 PubMed ID: 29417475DOI: 10.1007/s12602-018-9390-2Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study examines the preventive potential of the antimicrobial proteinaceous substance, Enterocin M, in horses and presents initial positive impacts on the animal’s gastrointestinal microbes.

Objective of the Research

  • The primary focus of this research was to investigate the potential benefits of Administering Enterocin M, an antimicrobial proteinaceous substance known as bacteriocin, in horses. The experiment aimed to explore the prophylactic potential of this substance for animal breeding, particularly examining its effects on horses which were clinically healthy. Enterocin M had not been previously applied to horses, making this study pioneering in its field.

Methodology

  • This study was a pilot experiment involving 10 clinically healthy horses, which were kept at the University of Veterinary Medicine and Pharmacy, Košice, Slovakia, after obtaining the necessary ethical approvals.
  • The horses were provided a regulated diet of hay and oats, or alternatively allowed to graze, with unrestricted access to water.
  • The experiment followed a timeline of 6 weeks, during which sampling was carried out at the beginning of the study (day 0-1), three weeks after the application of Enterocin M (day 21), and three weeks following the cessation (day 42).
  • Fecal samples were collected directly from the rectum while blood samples were taken from the jugular vein. Each horse served as a control animal, compared to its status at the onset of the experiment (day 0-1).
  • Prior to sample collection, every horse was administered 100 μl of Enterocin M (Ent M), in a small feed bolus, to guarantee that it was consumed.

Scientific Analysis

  • The fecal samples that were collected were evaluated using the standard microbial dilution method. In addition, the metabolic properties and biochemistry were tested employing commercial kits and routine methods. Phagocytic (PA) activity was assessed with standard and flow cytometry.

Results and Conclusion

  • The administration of Ent M led to a substantial reduction in coliforms, campylobacters, and Clostridium spp. in the fecal matter of the horses – highlighting its potential to significantly influence the gastrointestinal bacteria of the animals.
  • In addition, an increase in phagocytic activity (PA) values was observed, indicative of possibly enhanced immune response in the horses.
  • The researchers also noted that Ent M showed no negative impact on the hydrolytic enzyme profile or biochemical blood parameters of the animals.
  • The overall findings suggest that Enterocin M holds potential for preventative applications and beneficial impacts on gut health in horses. However, more extensive studies would be necessary for a comprehensive understanding of its effects and benefits.

Cite This Article

APA
Lauková A, Styková E, Kubašová I, Gancarčíková S, Plachá I, Mudroňová D, Kandričáková A, Miltko R, Belzecki G, Valocký I, Strompfová V. (2018). Enterocin M and its Beneficial Effects in Horses-a Pilot Experiment. Probiotics Antimicrob Proteins, 10(3), 420-426. https://doi.org/10.1007/s12602-018-9390-2

Publication

Probiotics and antimicrobial proteins
ISSN: 1867-1314
NlmUniqueID: 101484100
Country: United States
Language: English
Volume: 10
Issue: 3
Pages: 420-426

Researcher Affiliations

Lauková, Andrea
  • Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia. laukova@saske.sk.
Styková, Eva
  • University of Veterinary Medicine and Pharmacy, Komenského 73, 048 03, Košice, Slovakia.
Kubašová, Ivana
  • Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Gancarčíková, Soňa
  • University of Veterinary Medicine and Pharmacy, Komenského 73, 048 03, Košice, Slovakia.
Plachá, Iveta
  • Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Mudroňová, Dagmar
  • University of Veterinary Medicine and Pharmacy, Komenského 73, 048 03, Košice, Slovakia.
Kandričáková, Anna
  • Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Miltko, Renata
  • The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Instytucka 3, 05-110, Jablonna, Poland.
Belzecki, Grzegorz
  • The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Instytucka 3, 05-110, Jablonna, Poland.
Valocký, Igor
  • University of Veterinary Medicine and Pharmacy, Komenského 73, 048 03, Košice, Slovakia.
Strompfová, Viola
  • Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / metabolism
  • Bridged-Ring Compounds / administration & dosage
  • Bridged-Ring Compounds / metabolism
  • Enterococcus faecium / chemistry
  • Enterococcus faecium / metabolism
  • Feces / microbiology
  • Female
  • Horse Diseases / microbiology
  • Horse Diseases / prevention & control
  • Horses
  • Male
  • Pilot Projects
  • Probiotics / administration & dosage
  • Probiotics / metabolism
  • Pseudomonas / drug effects
  • Pseudomonas / growth & development
  • Pseudomonas Infections / microbiology
  • Pseudomonas Infections / prevention & control
  • Pseudomonas Infections / veterinary
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / prevention & control
  • Staphylococcal Infections / veterinary
  • Staphylococcus / drug effects
  • Staphylococcus / growth & development

Grant Funding

  • vega 2/0012/16 / Slovak Scientific Agency Vega

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Citations

This article has been cited 10 times.
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