FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Parasitol Res / Enzyme-linked immunosorbent assays for the detection of equi...
Parasitology research2011; 110(2); 1001-1007; doi: 10.1007/s00436-011-2587-x

Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to a recombinant Fasciola hepatica surface antigen in an endemic area.

Abstract: The utility of an enzyme-linked immunosorbent assay to determine the sensitization against the trematode Fasciola hepatica in horses from an endemic area (NW Spain) was assessed. Blood samples were collected from 536 horses and tested against a 2.9-kDa recombinant surface protein (FhrAPS) to estimate the presence of IgG antibodies. Data were analysed regarding several intrinsic (age, gender and breed) and extrinsic factors (aptitude and housing). The farm size (number of horses/farm) was also considered. Sixty percent (95% CI 56, 64) of the horses were positive to the FhrAPS-ELISA, with a significantly higher seroprevalence in the mares (67%). Foals reached the lowest percentage of sensitization against the trematode (12%), and a significant positive correlation between the seroprevalence of fasciolosis and the age of the horses was established. When considering all the factors together, the seroprevalence of fasciolosis was initially classified into two groups (nodes) regarding the age of the horses. The node composed of the horses older than 1 year was then divided into two other clusters according to their gender. The mares were finally classified and grouped into two nodes regarding their breed. We concluded that the FhrAPS-ELISA is very useful for the demonstration of specific equine IgG antibodies against F. hepatica. An elevated risk of exposition to this trematode in horses maintained in endemic areas was proven. The possible role of horses as reservoirs for F. hepatica infections is discussed.
Get Full Text
Publication Date: 2011-08-17 PubMed ID: 21847600DOI: 10.1007/s00436-011-2587-xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Evaluation Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research assesses the effectiveness of a specific enzyme-linked immunosorbent assay to detect antibodies in horses, specific to a surface antigen of Fasciola hepatica, a liver fluke parasite common in an endemic area in Spain.

Introduction and Objectives

  • The researchers aimed to assess the utility of an enzyme-linked immunosorbent assay (ELISA), a common laboratory technique used to measure antibodies or antigens in a sample, for determining sensitization against Fasciola hepatica in horses. Fasciola hepatica, commonly known as the liver fluke, is a parasitic trematode affecting many species worldwide.

Methodology

  • 536 horses from an endemic area in Northwest Spain were checked for sensitization against Fasciola hepatica using an ELISA test that detects specific immune responses to a 2.9-kDa recombinant surface protein (FhrAPS) of the parasite.
  • Several factors were taken into account for data analysis, such as age, gender, breed, housing conditions, and farm size.

Results

  • About 60% of the horses tested positive for FhrAPS-ELISA, indicating exposure to Fasciola hepatica.
  • There were variations within this percentage based on age, gender and breed – with a 67% prevalence among mares, and the lowest (12%) among foals.
  • The study found a significant positive correlation between the prevalence of fasciolosis and the age of the horses.

Sub-Group Analysis

  • When considering all the factors together, the overall data could be divided into subgroups, or “nodes”, with findings revealing age to be the primary factor.
  • The group or node composed of horses older than 1 year could be further divided based on gender, and then mares could also be classified further based on breed.

Conclusion

  • The FhrAPS-ELISA was deemed very useful for demonstrating the presence of specific equine IgG antibodies against Fasciola hepatica. This indicates a high risk of exposure to this parasite in horses maintained in endemic areas.
  • The study also discusses the possible implication of the horses serving as reservoirs for F. hepatica infections.

Cite This Article

APA
Arias MS, Piñeiro P, Hillyer GV, Francisco I, Cazapal-Monteiro CF, Suárez JL, Morrondo P, Sánchez-Andrade R, Paz-Silva A. (2011). Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to a recombinant Fasciola hepatica surface antigen in an endemic area. Parasitol Res, 110(2), 1001-1007. https://doi.org/10.1007/s00436-011-2587-x

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 110
Issue: 2
Pages: 1001-1007

Researcher Affiliations

Arias, María Sol
  • Equine Diseases Study Group (Epidemiology, Parasitology and Zoonoses), Animal Pathology Department, Veterinary Faculty, Santiago de Compostela University, 27002 Lugo, Spain.
Piñeiro, Pablo
    Hillyer, George V
      Francisco, Iván
        Cazapal-Monteiro, Cristiana Filipa
          Suárez, José Luis
            Morrondo, Patrocinio
              Sánchez-Andrade, Rita
                Paz-Silva, Adolfo

                  MeSH Terms

                  • Animals
                  • Antibodies, Helminth / blood
                  • Antigens, Helminth
                  • Clinical Laboratory Techniques / methods
                  • Enzyme-Linked Immunosorbent Assay / methods
                  • Fasciola hepatica / immunology
                  • Fasciola hepatica / isolation & purification
                  • Fascioliasis / diagnosis
                  • Fascioliasis / parasitology
                  • Fascioliasis / veterinary
                  • Female
                  • Horse Diseases / diagnosis
                  • Horse Diseases / parasitology
                  • Horses
                  • Immunoglobulin G / blood
                  • Male
                  • Recombinant Proteins
                  • Seroepidemiologic Studies
                  • Sex Factors
                  • Spain
                  • Veterinary Medicine / methods

                  References

                  This article includes 26 references
                  1. Phiri IK, Phiri AM, Harrison LJ. Serum antibody isotype responses of Fasciola-infected sheep and cattle to excretory and secretory products of Fasciola species.. Vet Parasitol 2006 Nov 5;141(3-4):234-42.
                    pubmed: 16797844doi: 10.1016/j.vetpar.2006.05.019google scholar: lookup
                  2. Arias M, Lomba C, Dacal V, Vázquez L, Pedreira J, Francisco I, Piñeiro P, Cazapal-Monteiro C, Suárez JL, Díez-Baños P, Morrondo P, Sánchez-Andrade R, Paz-Silva A. Prevalence of mixed trematode infections in an abattoir receiving cattle from northern Portugal and north-west Spain.. Vet Rec 2011 Apr 16;168(15):408.
                    pubmed: 21493444doi: 10.1136/vr.d85google scholar: lookup
                  3. Gajewska A, Smaga-Kozłowska K, Wiśniewski M. [Pathological changes of liver in infection of Fasciola hepatica].. Wiad Parazytol 2005;51(2):115-23.
                    pubmed: 16838620
                  4. Sánchez-Andrade R, Paz-Silva A, Suárez J, Panadero R, Díez-Baños P, Morrondo P. Use of a sandwich-enzyme-linked immunosorbent assay (SEA) for the diagnosis of natural Fasciola hepatica infection in cattle from Galicia (NW Spain).. Vet Parasitol 2000 Nov 1;93(1):39-46.
                    pubmed: 11027859doi: 10.1016/s0304-4017(00)00326-5google scholar: lookup
                  5. Ross JG. A five-year study of the epidemiology of fascioliasis in the North, East and West of Scotland.. Br Vet J 1977 May-Jun;133(3):263-72.
                    pubmed: 608065doi: 10.1016/s0007-1935(17)34088-5google scholar: lookup
                  6. Morsy TA, Salem HS, Haridy FM, Rifaat MM, Abo-Zenadah NY, Adel el-Kadi M. Farm animals' fascioliasis in Ezbet El-Bakly (Tamyia Center) Al-Fayoum Governorate.. J Egypt Soc Parasitol 2005 Dec;35(3):825-32.
                    pubmed: 16333892
                  7. Gorman T, Aballay J, Fredes F, Silva M, Aguillón JC, Alcaíno HA. Immunodiagnosis of fasciolosis in horses and pigs using western blots.. Int J Parasitol 1997 Nov;27(11):1429-32.
                    pubmed: 9421735doi: 10.1016/s0020-7519(97)00090-8google scholar: lookup
                  8. Rodríguez-Pérez J, Hillyer GV. Detection of excretory-secretory circulating antigens in sheep infected with Fasciola hepatica and with Schistosoma mansoni and F. hepatica.. Vet Parasitol 1995 Jan;56(1-3):57-66.
                    pubmed: 7632228doi: 10.1016/0304-4017(94)00666-zgoogle scholar: lookup
                  9. Soulé C, Boulard C, Levieux D, Barnouin J, Plateau E. [Experimental equine fascioliasis: evolution of serologic, enzymatic and parasitic parameters].. Ann Rech Vet 1989;20(3):295-307.
                    pubmed: 2573310
                  10. Arias M, Piñeiro P, Hillyer GV, Suárez JL, Francisco I, Cortiñas FJ, Díez-Baños P, Morrondo P, Sánchez-Andrade R, Paz-Silva A. An approach of the laboratory to the field: assessment of the influence of cattle management on the seroprevalence of fascioliasis by using polyclonal- and recombinant-based ELISAs.. J Parasitol 2010 Jun;96(3):626-31.
                    pubmed: 19925039doi: 10.1645/GE-2333.1google scholar: lookup
                  11. Arias MS, Suárez JL, Hillyer GV, Francisco I, Calvo E, Sánchez-Andrade R, Díaz P, Francisco R, Díez-Baños P, Morrondo P, Paz-Silva A. A recombinant-based ELISA evaluating the efficacy of netobimin and albendazole in ruminants with naturally acquired fascioliasis.. Vet J 2009 Oct;182(1):73-8.
                    pubmed: 19524206doi: 10.1016/j.tvjl.2008.05.015google scholar: lookup
                  12. Perrucci S, Pinello E, Fichi G, Ciardi E, Bàrberi P, Moonen C, Ragaglini G, Bibbiani C. Parasitic infections in an organic grazing cattle herd in Tuscany using geographic information systems to determine risk factors.. Vet Ital 2007 Jul-Sep;43(3):415-24.
                    pubmed: 20422517
                  13. Haridy FM, Morsy TA, Gawish NI, Antonios TN, Abdel Gawad AG. The potential reservoir role of donkeys and horses in zoonotic fascioliasis in Gharbia Governorate, Egypt.. J Egypt Soc Parasitol 2002 Aug;32(2):561-70, 1 p following 570.
                    pubmed: 12214933
                  14. Espino AM, Díaz A, Pérez A, Finlay CM. Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak.. J Clin Microbiol 1998 Sep;36(9):2723-6.
                    pubmed: 9705421doi: 10.1128/JCM.36.9.2723-2726.1998google scholar: lookup
                  15. Lean I, Westwood C, Playford M. Livestock disease threats associated with intensification of pastoral dairy farming.. N Z Vet J 2008 Dec;56(6):261-9.
                    pubmed: 19043462doi: 10.1080/00480169.2008.36845google scholar: lookup
                  16. Paz-Silva A, Sánchez-Andrade R, Suárez JL, Pedreira J, Arias M, López C, Panadero R, Díaz P, Díez-Baños P, Morrondo P. Prevalence of natural ovine fasciolosis shown by demonstrating the presence of serum circulating antigens.. Parasitol Res 2003 Oct;91(4):328-31.
                    pubmed: 14574565doi: 10.1007/s00436-003-0961-zgoogle scholar: lookup
                  17. Sánchez-Andrade R, Paz-Silva A, Suárez JL, Panadero R, Pedreira J, López C, Díez-Baños P, Morrondo P. Influence of age and breed on natural bovine fasciolosis in an endemic area (Galicia, NW Spain).. Vet Res Commun 2002 Jul;26(5):361-70.
                    pubmed: 12212726doi: 10.1023/a:1016290727793google scholar: lookup
                  18. Apt W, Aguilera X, Vega F, Alcaíno H, Zulantay I, Apt P, González V, Retamal C, Rodríguez J, Sandoval J. [Prevalence of fascioliasis in humans, horses, pigs, and wild rabbits in 3 Chilean provinces].. Bol Oficina Sanit Panam 1993 Nov;115(5):405-14.
                    pubmed: 8274227
                  19. Arias M, Morrondo P, Hillyer GV, Sánchez-Andrade R, Suárez JL, Lomba C, Pedreira J, Díaz P, Díez-Baños P, Paz-Silva A. Immunodiagnosis of current fasciolosis in sheep naturally exposed to Fasciola hepatica by using a 2.9 kDa recombinant protein.. Vet Parasitol 2007 May 15;146(1-2):46-9.
                    pubmed: 17350764doi: 10.1016/j.vetpar.2007.02.007google scholar: lookup
                  20. Carnevale S, Rodríguez MI, Guarnera EA, Carmona C, Tanos T, Angel SO. Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase.. Diagn Microbiol Infect Dis 2001 Sep-Oct;41(1-2):43-9.
                    pubmed: 11687313doi: 10.1016/s0732-8893(01)00288-7google scholar: lookup
                  21. Knubben-Schweizer G, Deplazes P, Torgerson PR, Rapsch C, Meli ML, Braun U. [Bovine fasciolosis in Switzerland: relevance and control].. Schweiz Arch Tierheilkd 2010 May;152(5):223-9.
                    pubmed: 20464681doi: 10.1024/0036-7281/a000053google scholar: lookup
                  22. Getachew M, Innocent GT, Trawford AF, Reid SW, Love S. Epidemiological features of fasciolosis in working donkeys in Ethiopia.. Vet Parasitol 2010 May 11;169(3-4):335-9.
                    pubmed: 20138432doi: 10.1016/j.vetpar.2010.01.007google scholar: lookup
                  23. Paz-Silva A, Hillyer GV, Sánchez-Andrade R, Rodríguez-Medina JR, Arias M, Morrondo P, Díez-Baños P. Isolation, identification and expression of a Fasciola hepatica cDNA encoding a 2.9-kDa recombinant protein for the diagnosis of ovine fasciolosis.. Parasitol Res 2005 Jan;95(2):129-35.
                    pubmed: 15609063doi: 10.1007/s00436-004-1202-9google scholar: lookup
                  24. Rogan WJ, Gladen B. Estimating prevalence from the results of a screening test.. Am J Epidemiol 1978 Jan;107(1):71-6.
                    pubmed: 623091doi: 10.1093/oxfordjournals.aje.a112510google scholar: lookup
                  25. Khan MK, Sajid MS, Khan MN, Iqbal Z, Iqbal MU. Bovine fasciolosis: prevalence, effects of treatment on productivity and cost benefit analysis in five districts of Punjab, Pakistan.. Res Vet Sci 2009 Aug;87(1):70-5.
                    pubmed: 19181352doi: 10.1016/j.rvsc.2008.12.013google scholar: lookup
                  26. Sánchez-Andrade R, Paz-Silva A, Suárez JL, Panadero R, Pedreira J, Díez-Baños P, Morrondo P. Effect of fasciolicides on the antigenaemia in sheep naturally infected with Fasciola hepatica.. Parasitol Res 2001 Aug;87(8):609-14.
                    pubmed: 11510995doi: 10.1007/s004360100425google scholar: lookup

                  Citations

                  This article has been cited 1 times.
                  1. Villa-Mancera A, Reynoso-Palomar A. The prevalence and risk factors of liver fluke infection in Mexican horses, donkeys and mules in tropical and temperate regions.. Parasitol Res 2020 Nov;119(11):3699-3703.
                    doi: 10.1007/s00436-020-06910-1pubmed: 33006039google scholar: lookup
                  Subscribe to our newsletter
                  Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.